YOL022C / TSR4

Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOL022C / TSR4

Click on Significant Values for Screen Details ID:SGTC_166|Compound:k072-0058|FD-Score:-2.85|P-value:5.31E-4 ID:SGTC_182|Compound:3448-4371|FD-Score:2.88|P-value:4.26E-4 ID:SGTC_186|Compound:3455-3465|FD-Score:2.72|P-value:8.22E-4 ID:SGTC_295|Compound:k015-0027|FD-Score:-3.61|P-value:1.66E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:-2.77|P-value:7.35E-4 ID:SGTC_333|Compound:3486-0131|FD-Score:2.80|P-value:5.98E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:2.74|P-value:7.55E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:-2.92|P-value:3.96E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:3.22|P-value:9.44E-5 ID:SGTC_386|Compound:1509-0011|FD-Score:3.34|P-value:5.49E-5 ID:SGTC_441|Compound:propafenone|FD-Score:-2.80|P-value:6.46E-4 ID:SGTC_459|Compound:nigericin|FD-Score:4.59|P-value:5.40E-8 ID:SGTC_469|Compound:w7|FD-Score:-4.07|P-value:1.41E-6 ID:SGTC_500|Compound:h-89|FD-Score:2.84|P-value:5.10E-4 ID:SGTC_725|Compound:1497-0963|FD-Score:3.21|P-value:1.00E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:5.30|P-value:4.37E-10 ID:SGTC_1017|Compound:4239-0030|FD-Score:-2.81|P-value:6.23E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.70|P-value:1.05E-5 ID:SGTC_1236|Compound:0330-0013|FD-Score:3.39|P-value:4.27E-5 ID:SGTC_1244|Compound:0416-0015|FD-Score:4.99|P-value:3.82E-9 ID:SGTC_1249|Compound:0481-0015|FD-Score:-2.72|P-value:8.82E-4 ID:SGTC_1287|Compound:0971-0001|FD-Score:2.86|P-value:4.70E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.03|P-value:2.44E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:2.73|P-value:7.72E-4 ID:SGTC_1626|Compound:st003712|FD-Score:-2.85|P-value:5.17E-4 ID:SGTC_1735|Compound:st034310|FD-Score:3.72|P-value:8.30E-6 ID:SGTC_1740|Compound:st038286|FD-Score:-3.03|P-value:2.42E-4 ID:SGTC_2126|Compound:5251174|FD-Score:2.78|P-value:6.30E-4 ID:SGTC_2269|Compound:7935389|FD-Score:-3.78|P-value:6.70E-6 ID:SGTC_2271|Compound:7849410|FD-Score:3.25|P-value:8.29E-5 ID:SGTC_2280|Compound:7785178|FD-Score:-6.33|P-value:1.52E-13 ID:SGTC_2289|Compound:7946404|FD-Score:-4.34|P-value:2.85E-7 ID:SGTC_2290|Compound:7904846|FD-Score:-3.81|P-value:5.73E-6 ID:SGTC_2357|Compound:9067248|FD-Score:-5.24|P-value:8.21E-10 ID:SGTC_2365|Compound:9042475|FD-Score:-3.23|P-value:1.03E-4 ID:SGTC_2379|Compound:9035213|FD-Score:2.75|P-value:7.15E-4 ID:SGTC_2431|Compound:5490079|FD-Score:2.75|P-value:7.21E-4 ID:SGTC_2479|Compound:5617353|FD-Score:3.07|P-value:1.91E-4 ID:SGTC_2521|Compound:angolensin|FD-Score:-2.86|P-value:5.04E-4 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:-3.00|P-value:2.82E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.70|P-value:9.24E-6 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:3.82|P-value:4.86E-6 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-5.63|P-value:4.29E-11 ID:SGTC_2695|Compound:st077686|FD-Score:-2.75|P-value:7.78E-4 ID:SGTC_2698|Compound:st077697|FD-Score:-3.89|P-value:3.87E-6 ID:SGTC_2725|Compound:haloperidol|FD-Score:-2.78|P-value:6.93E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:-4.80|P-value:1.60E-8 ID:SGTC_2756|Compound:carbadox|FD-Score:-3.31|P-value:7.01E-5 ID:SGTC_2785|Compound:7756463|FD-Score:-2.77|P-value:7.24E-4 ID:SGTC_2804|Compound:7954240|FD-Score:-3.73|P-value:9.06E-6 ID:SGTC_2808|Compound:7946468|FD-Score:3.59|P-value:1.59E-5 ID:SGTC_2810|Compound:7959871|FD-Score:3.16|P-value:1.28E-4 ID:SGTC_2811|Compound:7967822|FD-Score:-5.32|P-value:4.59E-10 ID:SGTC_2817|Compound:7995342|FD-Score:3.25|P-value:8.28E-5 ID:SGTC_2851|Compound:9015922|FD-Score:4.32|P-value:2.89E-7 ID:SGTC_2913|Compound:7966556|FD-Score:3.06|P-value:1.94E-4 ID:SGTC_2993|Compound:9054257|FD-Score:-2.97|P-value:3.12E-4 ID:SGTC_2996|Compound:9066192|FD-Score:-3.59|P-value:1.80E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-5.75|P-value:1.81E-11 ID:SGTC_3067|Compound:9117431|FD-Score:4.16|P-value:7.47E-7 ID:SGTC_3072|Compound:9116419|FD-Score:3.67|P-value:1.06E-5 ID:SGTC_3103|Compound:9120822|FD-Score:4.71|P-value:2.58E-8 ID:SGTC_3107|Compound:9121808|FD-Score:3.02|P-value:2.32E-4 ID:SGTC_3114|Compound:9122766|FD-Score:-3.42|P-value:4.17E-5 ID:SGTC_3116|Compound:9124943|FD-Score:-5.38|P-value:2.83E-10 ID:SGTC_3120|Compound:9124077|FD-Score:-2.73|P-value:8.40E-4 ID:SGTC_3192|Compound:9110362|FD-Score:2.72|P-value:8.02E-4 ID:SGTC_3211|Compound:9128781|FD-Score:2.86|P-value:4.53E-4 ID:SGTC_3223|Compound:9129970|FD-Score:3.34|P-value:5.46E-5 ID:SGTC_3227|Compound:9130768|FD-Score:3.08|P-value:1.79E-4 ID:SGTC_3228|Compound:9131799|FD-Score:2.67|P-value:9.94E-4 ID:SGTC_3347|Compound:9188721|FD-Score:2.90|P-value:3.89E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-2.85|P-value:5.31E-4 ID:SGTC_182|Compound:3448-4371|FD-Score:2.88|P-value:4.26E-4 ID:SGTC_186|Compound:3455-3465|FD-Score:2.72|P-value:8.22E-4 ID:SGTC_295|Compound:k015-0027|FD-Score:-3.61|P-value:1.66E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:-2.77|P-value:7.35E-4 ID:SGTC_333|Compound:3486-0131|FD-Score:2.80|P-value:5.98E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:2.74|P-value:7.55E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:-2.92|P-value:3.96E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:3.22|P-value:9.44E-5 ID:SGTC_386|Compound:1509-0011|FD-Score:3.34|P-value:5.49E-5 ID:SGTC_441|Compound:propafenone|FD-Score:-2.80|P-value:6.46E-4 ID:SGTC_459|Compound:nigericin|FD-Score:4.59|P-value:5.40E-8 ID:SGTC_469|Compound:w7|FD-Score:-4.07|P-value:1.41E-6 ID:SGTC_500|Compound:h-89|FD-Score:2.84|P-value:5.10E-4 ID:SGTC_725|Compound:1497-0963|FD-Score:3.21|P-value:1.00E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:5.30|P-value:4.37E-10 ID:SGTC_1017|Compound:4239-0030|FD-Score:-2.81|P-value:6.23E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.70|P-value:1.05E-5 ID:SGTC_1236|Compound:0330-0013|FD-Score:3.39|P-value:4.27E-5 ID:SGTC_1244|Compound:0416-0015|FD-Score:4.99|P-value:3.82E-9 ID:SGTC_1249|Compound:0481-0015|FD-Score:-2.72|P-value:8.82E-4 ID:SGTC_1287|Compound:0971-0001|FD-Score:2.86|P-value:4.70E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.03|P-value:2.44E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:2.73|P-value:7.72E-4 ID:SGTC_1626|Compound:st003712|FD-Score:-2.85|P-value:5.17E-4 ID:SGTC_1735|Compound:st034310|FD-Score:3.72|P-value:8.30E-6 ID:SGTC_1740|Compound:st038286|FD-Score:-3.03|P-value:2.42E-4 ID:SGTC_2126|Compound:5251174|FD-Score:2.78|P-value:6.30E-4 ID:SGTC_2269|Compound:7935389|FD-Score:-3.78|P-value:6.70E-6 ID:SGTC_2271|Compound:7849410|FD-Score:3.25|P-value:8.29E-5 ID:SGTC_2280|Compound:7785178|FD-Score:-6.33|P-value:1.52E-13 ID:SGTC_2289|Compound:7946404|FD-Score:-4.34|P-value:2.85E-7 ID:SGTC_2290|Compound:7904846|FD-Score:-3.81|P-value:5.73E-6 ID:SGTC_2357|Compound:9067248|FD-Score:-5.24|P-value:8.21E-10 ID:SGTC_2365|Compound:9042475|FD-Score:-3.23|P-value:1.03E-4 ID:SGTC_2379|Compound:9035213|FD-Score:2.75|P-value:7.15E-4 ID:SGTC_2431|Compound:5490079|FD-Score:2.75|P-value:7.21E-4 ID:SGTC_2479|Compound:5617353|FD-Score:3.07|P-value:1.91E-4 ID:SGTC_2521|Compound:angolensin|FD-Score:-2.86|P-value:5.04E-4 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:-3.00|P-value:2.82E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.70|P-value:9.24E-6 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:3.82|P-value:4.86E-6 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-5.63|P-value:4.29E-11 ID:SGTC_2695|Compound:st077686|FD-Score:-2.75|P-value:7.78E-4 ID:SGTC_2698|Compound:st077697|FD-Score:-3.89|P-value:3.87E-6 ID:SGTC_2725|Compound:haloperidol|FD-Score:-2.78|P-value:6.93E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:-4.80|P-value:1.60E-8 ID:SGTC_2756|Compound:carbadox|FD-Score:-3.31|P-value:7.01E-5 ID:SGTC_2785|Compound:7756463|FD-Score:-2.77|P-value:7.24E-4 ID:SGTC_2804|Compound:7954240|FD-Score:-3.73|P-value:9.06E-6 ID:SGTC_2808|Compound:7946468|FD-Score:3.59|P-value:1.59E-5 ID:SGTC_2810|Compound:7959871|FD-Score:3.16|P-value:1.28E-4 ID:SGTC_2811|Compound:7967822|FD-Score:-5.32|P-value:4.59E-10 ID:SGTC_2817|Compound:7995342|FD-Score:3.25|P-value:8.28E-5 ID:SGTC_2851|Compound:9015922|FD-Score:4.32|P-value:2.89E-7 ID:SGTC_2913|Compound:7966556|FD-Score:3.06|P-value:1.94E-4 ID:SGTC_2993|Compound:9054257|FD-Score:-2.97|P-value:3.12E-4 ID:SGTC_2996|Compound:9066192|FD-Score:-3.59|P-value:1.80E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-5.75|P-value:1.81E-11 ID:SGTC_3067|Compound:9117431|FD-Score:4.16|P-value:7.47E-7 ID:SGTC_3072|Compound:9116419|FD-Score:3.67|P-value:1.06E-5 ID:SGTC_3103|Compound:9120822|FD-Score:4.71|P-value:2.58E-8 ID:SGTC_3107|Compound:9121808|FD-Score:3.02|P-value:2.32E-4 ID:SGTC_3114|Compound:9122766|FD-Score:-3.42|P-value:4.17E-5 ID:SGTC_3116|Compound:9124943|FD-Score:-5.38|P-value:2.83E-10 ID:SGTC_3120|Compound:9124077|FD-Score:-2.73|P-value:8.40E-4 ID:SGTC_3192|Compound:9110362|FD-Score:2.72|P-value:8.02E-4 ID:SGTC_3211|Compound:9128781|FD-Score:2.86|P-value:4.53E-4 ID:SGTC_3223|Compound:9129970|FD-Score:3.34|P-value:5.46E-5 ID:SGTC_3227|Compound:9130768|FD-Score:3.08|P-value:1.79E-4 ID:SGTC_3228|Compound:9131799|FD-Score:2.67|P-value:9.94E-4 ID:SGTC_3347|Compound:9188721|FD-Score:2.90|P-value:3.89E-4

Top fitness defect scores for YOL022C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_777 1315-0167 5.30 517.0 µM 4.37E-10 Golgi 1 5.74E-8 1.41
2 SGTC_1244 0416-0015 4.99 52.5 µM 3.82E-9 ERG2 2 2.95E-7 1.12
3 SGTC_3103 9120822 4.71 49.5 µM 2.58E-8 4 1.25E-6 0.38
4 SGTC_459 nigericin 4.59 15.3 µM 5.40E-8 8 2.19E-6 0.46
5 SGTC_2851 9015922 4.32 71.4 µM 2.89E-7 8 7.78E-6 0.29
6 SGTC_3067 9117431 4.16 49.5 µM 7.47E-7 5 1.60E-5 0.03
7 SGTC_2597 laccaic acid a 3.82 100.0 µM 4.86E-6 7 6.62E-5 0.27
8 SGTC_1735 st034310 3.72 56.8 µM 8.30E-6 Golgi 13 9.94E-5 0.27
9 SGTC_2594 a-mangostin 3.70 100.0 µM 9.24E-6 9 1.08E-4 0.02
10 SGTC_3072 9116419 3.67 49.5 µM 1.06E-5 ERG2 6 1.19E-4 0.04
11 SGTC_2808 7946468 3.59 45.5 µM 1.59E-5 11 1.63E-4 0.01
12 SGTC_1236 0330-0013 3.39 258.0 µM 4.27E-5 6 3.45E-4 0.53
13 SGTC_3223 9129970 3.34 49.5 µM 5.46E-5 11 4.16E-4 0.10
14 SGTC_386 1509-0011 3.34 3.1 µM 5.49E-5 12 4.18E-4 0.30
15 SGTC_2817 7995342 3.25 71.4 µM 8.28E-5 14 5.71E-4 0.13
16 SGTC_2271 7849410 3.25 118.6 µM 8.29E-5 4 5.72E-4 0.04
17 SGTC_380 3970-0968 3.22 6.0 µM 9.44E-5 8 6.31E-4 0.04
18 SGTC_725 1497-0963 3.21 19.0 µM 1.00E-4 copper-dependent oxidative stress 7 6.61E-4 0.07
19 SGTC_2810 7959871 3.16 45.5 µM 1.28E-4 copper-dependent oxidative stress 24 7.98E-4 0.09
20 SGTC_3227 9130768 3.08 49.5 µM 1.79E-4 3 0.00103 0.15

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.133 8.88E-15 YAR008W SEN34 het Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
0.121 1.72E-12 YER172C BRR2 het RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
0.115 2.09E-11 YEL004W YEA4 hom Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER
0.113 4.84E-11 YKL088W CAB3 het Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC
0.109 2.82E-10 YKL083W_d YKL083W_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
0.104 1.62E-9 YGR236C SPG1 hom Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.102 3.76E-9 YMR150C IMP1 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.099 9.06E-9 YBR262C AIM5 hom Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
0.094 5.53E-8 YJR147W HMS2 hom Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication
0.093 6.08E-8 YBR118W TEF2 hom Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication
0.091 1.13E-7 YGR207C CIR1 hom Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response
0.091 1.43E-7 YKL107W_p YKL107W_p hom Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein
0.087 4.00E-7 YBL020W RFT1 het Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein
0.087 4.86E-7 YLL012W YEH1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.087 5.03E-7 YOR227W HER1 hom Protein of unknown function required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication