YOL025W / LAG2

Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL025W / LAG2

Click on Significant Values for Screen Details ID:SGTC_11|Compound:0438-0307|FD-Score:-5.15|P-value:1.18E-7 ID:SGTC_23|Compound:0213-0011|FD-Score:4.86|P-value:6.72E-7 ID:SGTC_27|Compound:0485-0001|FD-Score:3.18|P-value:7.94E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:-3.11|P-value:8.88E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.33|P-value:4.81E-4 ID:SGTC_271|Compound:rotenone|FD-Score:-3.19|P-value:6.56E-4 ID:SGTC_433|Compound:pimozide|FD-Score:-5.91|P-value:1.54E-9 ID:SGTC_523|Compound:1934-0222|FD-Score:3.60|P-value:1.75E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:3.24|P-value:6.57E-4 ID:SGTC_583|Compound:1488-1091|FD-Score:4.82|P-value:8.19E-7 ID:SGTC_588|Compound:3970-1144|FD-Score:-3.38|P-value:3.36E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:-3.73|P-value:8.75E-5 ID:SGTC_745|Compound:0469-0705|FD-Score:-3.13|P-value:8.28E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:-3.28|P-value:4.83E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.27|P-value:5.81E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:-3.60|P-value:1.49E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:3.14|P-value:9.16E-4 ID:SGTC_1724|Compound:st037281|FD-Score:-3.34|P-value:3.92E-4 ID:SGTC_1828|Compound:st055811|FD-Score:3.46|P-value:2.95E-4 ID:SGTC_1854|Compound:st056233|FD-Score:-3.50|P-value:2.15E-4 ID:SGTC_1868|Compound:st057644|FD-Score:8.38|P-value:3.35E-17 ID:SGTC_2009|Compound:4012297|FD-Score:3.61|P-value:1.72E-4 ID:SGTC_2083|Compound:5306046|FD-Score:3.13|P-value:9.41E-4 ID:SGTC_2138|Compound:5575593|FD-Score:-4.13|P-value:1.71E-5 ID:SGTC_2147|Compound:5545932|FD-Score:3.31|P-value:5.16E-4 ID:SGTC_2150|Compound:5556613|FD-Score:4.80|P-value:9.03E-7 ID:SGTC_2152|Compound:5568786|FD-Score:3.74|P-value:1.04E-4 ID:SGTC_2259|Compound:7964009|FD-Score:3.41|P-value:3.55E-4 ID:SGTC_2329|Compound:6138490|FD-Score:4.15|P-value:1.89E-5 ID:SGTC_2332|Compound:7982454|FD-Score:3.41|P-value:3.63E-4 ID:SGTC_2436|Compound:5633522|FD-Score:-3.20|P-value:6.43E-4 ID:SGTC_2526|Compound:patulin|FD-Score:-3.13|P-value:8.27E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:4.07|P-value:2.58E-5 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.52|P-value:2.40E-4 ID:SGTC_2678|Compound:equilin|FD-Score:-3.16|P-value:7.37E-4 ID:SGTC_2917|Compound:7979103|FD-Score:4.55|P-value:3.03E-6 ID:SGTC_2952|Compound:9073731|FD-Score:3.15|P-value:8.92E-4 ID:SGTC_3030|Compound:9087619|FD-Score:-3.31|P-value:4.34E-4 ID:SGTC_3032|Compound:9089427|FD-Score:3.96|P-value:4.12E-5 ID:SGTC_3132|Compound:9126706|FD-Score:-3.99|P-value:3.09E-5 ID:SGTC_3133|Compound:9127736|FD-Score:3.49|P-value:2.71E-4 ID:SGTC_3140|Compound:9094243|FD-Score:3.73|P-value:1.05E-4 ID:SGTC_3220|Compound:9131269|FD-Score:-6.32|P-value:1.21E-10 ID:SGTC_3246|Compound:9135460|FD-Score:3.26|P-value:6.13E-4 ID:SGTC_3284|Compound:9108171|FD-Score:4.54|P-value:3.26E-6 ID:SGTC_3306|Compound:9127676|FD-Score:3.69|P-value:1.25E-4 ID:SGTC_11|Compound:0438-0307|FD-Score:-5.15|P-value:1.18E-7 ID:SGTC_23|Compound:0213-0011|FD-Score:4.86|P-value:6.72E-7 ID:SGTC_27|Compound:0485-0001|FD-Score:3.18|P-value:7.94E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:-3.11|P-value:8.88E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.33|P-value:4.81E-4 ID:SGTC_271|Compound:rotenone|FD-Score:-3.19|P-value:6.56E-4 ID:SGTC_433|Compound:pimozide|FD-Score:-5.91|P-value:1.54E-9 ID:SGTC_523|Compound:1934-0222|FD-Score:3.60|P-value:1.75E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:3.24|P-value:6.57E-4 ID:SGTC_583|Compound:1488-1091|FD-Score:4.82|P-value:8.19E-7 ID:SGTC_588|Compound:3970-1144|FD-Score:-3.38|P-value:3.36E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:-3.73|P-value:8.75E-5 ID:SGTC_745|Compound:0469-0705|FD-Score:-3.13|P-value:8.28E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:-3.28|P-value:4.83E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.27|P-value:5.81E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:-3.60|P-value:1.49E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:3.14|P-value:9.16E-4 ID:SGTC_1724|Compound:st037281|FD-Score:-3.34|P-value:3.92E-4 ID:SGTC_1828|Compound:st055811|FD-Score:3.46|P-value:2.95E-4 ID:SGTC_1854|Compound:st056233|FD-Score:-3.50|P-value:2.15E-4 ID:SGTC_1868|Compound:st057644|FD-Score:8.38|P-value:3.35E-17 ID:SGTC_2009|Compound:4012297|FD-Score:3.61|P-value:1.72E-4 ID:SGTC_2083|Compound:5306046|FD-Score:3.13|P-value:9.41E-4 ID:SGTC_2138|Compound:5575593|FD-Score:-4.13|P-value:1.71E-5 ID:SGTC_2147|Compound:5545932|FD-Score:3.31|P-value:5.16E-4 ID:SGTC_2150|Compound:5556613|FD-Score:4.80|P-value:9.03E-7 ID:SGTC_2152|Compound:5568786|FD-Score:3.74|P-value:1.04E-4 ID:SGTC_2259|Compound:7964009|FD-Score:3.41|P-value:3.55E-4 ID:SGTC_2329|Compound:6138490|FD-Score:4.15|P-value:1.89E-5 ID:SGTC_2332|Compound:7982454|FD-Score:3.41|P-value:3.63E-4 ID:SGTC_2436|Compound:5633522|FD-Score:-3.20|P-value:6.43E-4 ID:SGTC_2526|Compound:patulin|FD-Score:-3.13|P-value:8.27E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:4.07|P-value:2.58E-5 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.52|P-value:2.40E-4 ID:SGTC_2678|Compound:equilin|FD-Score:-3.16|P-value:7.37E-4 ID:SGTC_2917|Compound:7979103|FD-Score:4.55|P-value:3.03E-6 ID:SGTC_2952|Compound:9073731|FD-Score:3.15|P-value:8.92E-4 ID:SGTC_3030|Compound:9087619|FD-Score:-3.31|P-value:4.34E-4 ID:SGTC_3032|Compound:9089427|FD-Score:3.96|P-value:4.12E-5 ID:SGTC_3132|Compound:9126706|FD-Score:-3.99|P-value:3.09E-5 ID:SGTC_3133|Compound:9127736|FD-Score:3.49|P-value:2.71E-4 ID:SGTC_3140|Compound:9094243|FD-Score:3.73|P-value:1.05E-4 ID:SGTC_3220|Compound:9131269|FD-Score:-6.32|P-value:1.21E-10 ID:SGTC_3246|Compound:9135460|FD-Score:3.26|P-value:6.13E-4 ID:SGTC_3284|Compound:9108171|FD-Score:4.54|P-value:3.26E-6 ID:SGTC_3306|Compound:9127676|FD-Score:3.69|P-value:1.25E-4

Top fitness defect scores for YOL025W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1868 st057644 8.38 42.7 µM 3.35E-17 2 2.56E-17
2 SGTC_23 0213-0011 4.86 40.0 µM 6.72E-7 copper-dependent oxidative stress 13 5.84E-7
3 SGTC_583 1488-1091 4.82 88.9 µM 8.19E-7 9 7.12E-7
4 SGTC_2150 5556613 4.80 200.0 µM 9.03E-7 6 7.86E-7
5 SGTC_2917 7979103 4.55 53.8 µM 3.03E-6 16 2.65E-6
6 SGTC_3284 9108171 4.54 64.9 µM 3.26E-6 17 2.86E-6
7 SGTC_2329 6138490 4.15 200.0 µM 1.89E-5 25 1.68E-5
8 SGTC_2539 epi(13)torulosol 4.07 80.0 µM 2.58E-5 23 2.30E-5
9 SGTC_3032 9089427 3.96 49.5 µM 4.12E-5 60S ribosome export 16 3.68E-5
10 SGTC_2152 5568786 3.74 200.0 µM 1.04E-4 14 9.38E-5
11 SGTC_3140 9094243 3.73 49.5 µM 1.05E-4 29 9.43E-5
12 SGTC_3306 9127676 3.69 59.8 µM 1.25E-4 29 1.12E-4
13 SGTC_2009 4012297 3.61 12.1 µM 1.72E-4 cell wall signaling 76 1.56E-4
14 SGTC_523 1934-0222 3.60 13.7 µM 1.75E-4 40 1.58E-4
15 SGTC_2611 pyromycin 3.52 23.9 µM 2.40E-4 50 2.17E-4
16 SGTC_3133 9127736 3.49 49.5 µM 2.71E-4 36 2.46E-4
17 SGTC_1828 st055811 3.46 49.0 µM 2.95E-4 16 2.67E-4
18 SGTC_2259 7964009 3.41 200.0 µM 3.55E-4 superoxide 51 3.23E-4
19 SGTC_2332 7982454 3.41 138.9 µM 3.63E-4 79 3.30E-4
20 SGTC_268 camptothecin 3.33 39.6 µM 4.81E-4 DNA damage response 113 4.38E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.112 6.75E-11 YGL232W TAN1 hom Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress
0.083 1.32E-6 YKL069W YKL069W hom Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress
0.078 5.64E-6 YGL009C LEU1 hom Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway
0.077 7.21E-6 YKL005C BYE1 hom Negative regulator of transcription elongation, contains a TFIIS-like domain and a PHD finger, multicopy suppressor of temperature-sensitive ess1 mutations, probably binds RNA polymerase II large subunit
0.076 1.00E-5 YOR040W GLO4 hom Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate
0.074 1.60E-5 YBR254C TRS20 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder
0.074 1.94E-5 YHR033W_p YHR033W_p hom Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YHR033W has a paralog, PRO1, that arose from the whole genome duplication
0.072 3.12E-5 YBR084C-A RPL19A hom Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication
0.069 5.69E-5 YOL066C RIB2 het Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs
0.069 7.19E-5 YGL025C PGD1 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
0.068 7.72E-5 YMR317W_p YMR317W_p hom Putative protein of unknown function with some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene
0.068 8.82E-5 YGL157W ARI1 hom NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily
0.067 9.26E-5 YJR140C HIR3 hom Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein
0.067 1.02E-4 YDL039C PRM7 hom Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements
0.066 1.39E-4 YLR327C TMA10 hom Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress