YOL031C / SIL1

Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL031C / SIL1

Click on Significant Values for Screen Details ID:SGTC_229|Compound:benomyl|FD-Score:-3.43|P-value:1.71E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:2.95|P-value:9.77E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.49|P-value:1.25E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:4.08|P-value:9.54E-6 ID:SGTC_323|Compound:4013-0906|FD-Score:3.54|P-value:1.05E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:-3.44|P-value:1.68E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:6.74|P-value:8.37E-13 ID:SGTC_427|Compound:1354-0053|FD-Score:-3.00|P-value:8.82E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:4.39|P-value:2.15E-6 ID:SGTC_505|Compound:gw-5074|FD-Score:3.18|P-value:4.28E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:4.87|P-value:1.65E-7 ID:SGTC_520|Compound:3584-0023|FD-Score:3.18|P-value:4.19E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:4.75|P-value:3.26E-7 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:3.18|P-value:4.31E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:3.58|P-value:8.67E-5 ID:SGTC_686|Compound:1319-0111|FD-Score:3.52|P-value:1.11E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:4.48|P-value:1.33E-6 ID:SGTC_814|Compound:3342-0569|FD-Score:3.25|P-value:3.28E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:-3.39|P-value:2.00E-4 ID:SGTC_868|Compound:0527-0199|FD-Score:4.21|P-value:4.98E-6 ID:SGTC_874|Compound:0599-0792|FD-Score:-3.09|P-value:6.22E-4 ID:SGTC_1064|Compound:honokiol|FD-Score:2.98|P-value:8.71E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:5.04|P-value:6.29E-8 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.11|P-value:5.50E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:3.81|P-value:3.26E-5 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.22|P-value:3.63E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:2.97|P-value:9.31E-4 ID:SGTC_1649|Compound:st011737|FD-Score:3.17|P-value:4.38E-4 ID:SGTC_1674|Compound:st016616|FD-Score:3.60|P-value:7.91E-5 ID:SGTC_1683|Compound:st010134|FD-Score:-3.19|P-value:4.28E-4 ID:SGTC_1705|Compound:st027868|FD-Score:3.05|P-value:6.86E-4 ID:SGTC_1929|Compound:genistein trimethyl ether|FD-Score:5.56|P-value:2.80E-9 ID:SGTC_1932|Compound:st074806|FD-Score:-4.50|P-value:1.31E-6 ID:SGTC_2019|Compound:4016004|FD-Score:4.42|P-value:1.84E-6 ID:SGTC_2055|Compound:5235184|FD-Score:3.49|P-value:1.26E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.19|P-value:4.38E-4 ID:SGTC_2302|Compound:7771753|FD-Score:-3.06|P-value:7.13E-4 ID:SGTC_2318|Compound:6029776|FD-Score:3.19|P-value:4.08E-4 ID:SGTC_2336|Compound:9001819|FD-Score:4.50|P-value:1.22E-6 ID:SGTC_2373|Compound:9071805|FD-Score:4.15|P-value:6.65E-6 ID:SGTC_2428|Compound:5615643|FD-Score:3.55|P-value:9.85E-5 ID:SGTC_2434|Compound:5670830|FD-Score:-3.08|P-value:6.48E-4 ID:SGTC_2471|Compound:5466956|FD-Score:-3.40|P-value:1.91E-4 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:-3.29|P-value:2.95E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:3.07|P-value:6.50E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:3.14|P-value:4.91E-4 ID:SGTC_2792|Compound:7535235|FD-Score:5.30|P-value:1.39E-8 ID:SGTC_2795|Compound:7780010|FD-Score:-3.69|P-value:5.87E-5 ID:SGTC_2799|Compound:7812892|FD-Score:3.28|P-value:2.87E-4 ID:SGTC_2833|Compound:9001315|FD-Score:3.74|P-value:4.42E-5 ID:SGTC_2921|Compound:7974240|FD-Score:5.10|P-value:4.66E-8 ID:SGTC_2943|Compound:9068529|FD-Score:3.13|P-value:5.09E-4 ID:SGTC_3074|Compound:9116018|FD-Score:-4.34|P-value:2.88E-6 ID:SGTC_3077|Compound:9117319|FD-Score:-4.84|P-value:2.12E-7 ID:SGTC_3080|Compound:9117754|FD-Score:-3.77|P-value:4.10E-5 ID:SGTC_3088|Compound:9117578|FD-Score:3.43|P-value:1.63E-4 ID:SGTC_3108|Compound:9121785|FD-Score:3.10|P-value:5.81E-4 ID:SGTC_3131|Compound:9127911|FD-Score:4.14|P-value:7.24E-6 ID:SGTC_3276|Compound:9139060|FD-Score:3.10|P-value:5.76E-4 ID:SGTC_3343|Compound:9148379|FD-Score:-3.00|P-value:8.58E-4 ID:SGTC_229|Compound:benomyl|FD-Score:-3.43|P-value:1.71E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:2.95|P-value:9.77E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.49|P-value:1.25E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:4.08|P-value:9.54E-6 ID:SGTC_323|Compound:4013-0906|FD-Score:3.54|P-value:1.05E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:-3.44|P-value:1.68E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:6.74|P-value:8.37E-13 ID:SGTC_427|Compound:1354-0053|FD-Score:-3.00|P-value:8.82E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:4.39|P-value:2.15E-6 ID:SGTC_505|Compound:gw-5074|FD-Score:3.18|P-value:4.28E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:4.87|P-value:1.65E-7 ID:SGTC_520|Compound:3584-0023|FD-Score:3.18|P-value:4.19E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:4.75|P-value:3.26E-7 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:3.18|P-value:4.31E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:3.58|P-value:8.67E-5 ID:SGTC_686|Compound:1319-0111|FD-Score:3.52|P-value:1.11E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:4.48|P-value:1.33E-6 ID:SGTC_814|Compound:3342-0569|FD-Score:3.25|P-value:3.28E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:-3.39|P-value:2.00E-4 ID:SGTC_868|Compound:0527-0199|FD-Score:4.21|P-value:4.98E-6 ID:SGTC_874|Compound:0599-0792|FD-Score:-3.09|P-value:6.22E-4 ID:SGTC_1064|Compound:honokiol|FD-Score:2.98|P-value:8.71E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:5.04|P-value:6.29E-8 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.11|P-value:5.50E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:3.81|P-value:3.26E-5 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.22|P-value:3.63E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:2.97|P-value:9.31E-4 ID:SGTC_1649|Compound:st011737|FD-Score:3.17|P-value:4.38E-4 ID:SGTC_1674|Compound:st016616|FD-Score:3.60|P-value:7.91E-5 ID:SGTC_1683|Compound:st010134|FD-Score:-3.19|P-value:4.28E-4 ID:SGTC_1705|Compound:st027868|FD-Score:3.05|P-value:6.86E-4 ID:SGTC_1929|Compound:genistein trimethyl ether|FD-Score:5.56|P-value:2.80E-9 ID:SGTC_1932|Compound:st074806|FD-Score:-4.50|P-value:1.31E-6 ID:SGTC_2019|Compound:4016004|FD-Score:4.42|P-value:1.84E-6 ID:SGTC_2055|Compound:5235184|FD-Score:3.49|P-value:1.26E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.19|P-value:4.38E-4 ID:SGTC_2302|Compound:7771753|FD-Score:-3.06|P-value:7.13E-4 ID:SGTC_2318|Compound:6029776|FD-Score:3.19|P-value:4.08E-4 ID:SGTC_2336|Compound:9001819|FD-Score:4.50|P-value:1.22E-6 ID:SGTC_2373|Compound:9071805|FD-Score:4.15|P-value:6.65E-6 ID:SGTC_2428|Compound:5615643|FD-Score:3.55|P-value:9.85E-5 ID:SGTC_2434|Compound:5670830|FD-Score:-3.08|P-value:6.48E-4 ID:SGTC_2471|Compound:5466956|FD-Score:-3.40|P-value:1.91E-4 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:-3.29|P-value:2.95E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:3.07|P-value:6.50E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:3.14|P-value:4.91E-4 ID:SGTC_2792|Compound:7535235|FD-Score:5.30|P-value:1.39E-8 ID:SGTC_2795|Compound:7780010|FD-Score:-3.69|P-value:5.87E-5 ID:SGTC_2799|Compound:7812892|FD-Score:3.28|P-value:2.87E-4 ID:SGTC_2833|Compound:9001315|FD-Score:3.74|P-value:4.42E-5 ID:SGTC_2921|Compound:7974240|FD-Score:5.10|P-value:4.66E-8 ID:SGTC_2943|Compound:9068529|FD-Score:3.13|P-value:5.09E-4 ID:SGTC_3074|Compound:9116018|FD-Score:-4.34|P-value:2.88E-6 ID:SGTC_3077|Compound:9117319|FD-Score:-4.84|P-value:2.12E-7 ID:SGTC_3080|Compound:9117754|FD-Score:-3.77|P-value:4.10E-5 ID:SGTC_3088|Compound:9117578|FD-Score:3.43|P-value:1.63E-4 ID:SGTC_3108|Compound:9121785|FD-Score:3.10|P-value:5.81E-4 ID:SGTC_3131|Compound:9127911|FD-Score:4.14|P-value:7.24E-6 ID:SGTC_3276|Compound:9139060|FD-Score:3.10|P-value:5.76E-4 ID:SGTC_3343|Compound:9148379|FD-Score:-3.00|P-value:8.58E-4

Top fitness defect scores for YOL031C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_426 0988-0037 6.74 14.6 µM 8.37E-13 ERAD & cell cycle 9 7.97E-12
2 SGTC_1929 genistein trimethyl ether 5.56 50.5 µM 2.80E-9 3 1.33E-8
3 SGTC_2792 7535235 5.30 81.8 µM 1.39E-8 fatty acid desaturase (OLE1) 15 5.74E-8
4 SGTC_2921 7974240 5.10 10.7 µM 4.66E-8 23 1.74E-7
5 SGTC_1068 loratadine 5.04 26.9 µM 6.29E-8 10 2.29E-7
6 SGTC_513 4245-1575 4.87 41.7 µM 1.65E-7 unfolded protein response 79 5.52E-7
7 SGTC_538 2922-0838 4.75 53.3 µM 3.26E-7 Golgi 16 1.03E-6
8 SGTC_2336 9001819 4.50 198.7 µM 1.22E-6 heme biosynthesis & mitochondrial translocase 32 3.44E-6
9 SGTC_779 1360-0071 4.48 233.0 µM 1.33E-6 fatty acid desaturase (OLE1) 17 3.74E-6
10 SGTC_2019 4016004 4.42 108.0 µM 1.84E-6 15 5.02E-6
11 SGTC_450 aminopyrene 4.39 156.0 nM 2.15E-6 23 5.80E-6
12 SGTC_868 0527-0199 4.21 213.0 µM 4.98E-6 fatty acid desaturase (OLE1) 22 1.25E-5
13 SGTC_2373 9071805 4.15 200.0 µM 6.65E-6 9 1.63E-5
14 SGTC_3131 9127911 4.14 49.5 µM 7.24E-6 25 1.76E-5
15 SGTC_255 tunicamycin 4.08 200.5 nM 9.54E-6 unfolded protein response 69 2.27E-5
16 SGTC_1095 3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran 3.81 15.5 µM 3.26E-5 29 6.99E-5
17 SGTC_2833 9001315 3.74 45.5 µM 4.42E-5 amide catabolism 65 9.23E-5
18 SGTC_1674 st016616 3.60 23.6 µM 7.91E-5 39 1.57E-4
19 SGTC_641 4092-0595 3.58 30.0 µM 8.67E-5 19 1.71E-4
20 SGTC_2428 5615643 3.55 7.8 µM 9.85E-5 39 1.92E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.123 7.98E-13 YNL043C_d YNL043C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W
0.117 9.88E-12 YBR243C ALG7 het UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.097 1.70E-8 YGR244C LSC2 hom Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate
0.088 2.96E-7 YBR256C RIB5 het Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway
0.087 3.97E-7 YJR117W STE24 hom Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
0.082 1.89E-6 YAL040C CLN3 hom G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis
0.081 2.82E-6 YGR208W SER2 hom Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
0.078 5.76E-6 YDR434W GPI17 het Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog
0.078 6.09E-6 YDR527W RBA50 het Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
0.076 9.88E-6 YBR150C TBS1 hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication
0.076 1.08E-5 YMR136W GAT2 hom Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine
0.075 1.23E-5 YDR248C_p YDR248C_p hom Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1
0.075 1.56E-5 YMR278W PGM3 hom Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential
0.074 1.77E-5 YOL063C CRT10 hom Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat
0.074 1.81E-5 YNL129W NRK1 hom Nicotinamide riboside kinase, catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis