YOL034W / SMC5

Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOL034W / SMC5

Click on Significant Values for Screen Details ID:SGTC_86|Compound:0417-1630|FD-Score:-3.94|P-value:5.24E-6 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.58|P-value:2.25E-10 ID:SGTC_206|Compound:4546-0025|FD-Score:2.81|P-value:9.93E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-6.14|P-value:3.65E-12 ID:SGTC_697|Compound:0508-1955|FD-Score:2.87|P-value:7.85E-4 ID:SGTC_741|Compound:4582-0002|FD-Score:-2.79|P-value:8.71E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:3.13|P-value:2.79E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:-3.13|P-value:2.24E-4 ID:SGTC_1224|Compound:0823-0416|FD-Score:-2.76|P-value:9.70E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:-3.07|P-value:2.87E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:3.76|P-value:1.68E-5 ID:SGTC_1276|Compound:0871-0068|FD-Score:-3.32|P-value:9.92E-5 ID:SGTC_1379|Compound:2144-0755|FD-Score:3.31|P-value:1.30E-4 ID:SGTC_1388|Compound:3346-4487|FD-Score:3.45|P-value:7.04E-5 ID:SGTC_1524|Compound:corynanthine|FD-Score:-15.60|P-value:1.40E-67 ID:SGTC_1526|Compound:st051518|FD-Score:7.26|P-value:4.36E-16 ID:SGTC_1788|Compound:fluconazole|FD-Score:6.61|P-value:1.31E-13 ID:SGTC_1914|Compound:st062367|FD-Score:-3.09|P-value:2.61E-4 ID:SGTC_1932|Compound:st074806|FD-Score:-2.84|P-value:7.27E-4 ID:SGTC_1968|Compound:st069590|FD-Score:3.23|P-value:1.89E-4 ID:SGTC_1991|Compound:st073000|FD-Score:3.26|P-value:1.65E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-3.12|P-value:2.35E-4 ID:SGTC_2094|Compound:5332512|FD-Score:3.87|P-value:9.60E-6 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.74|P-value:1.82E-5 ID:SGTC_2135|Compound:5326629|FD-Score:-3.50|P-value:4.47E-5 ID:SGTC_2247|Compound:cerulenin|FD-Score:-3.00|P-value:3.84E-4 ID:SGTC_2260|Compound:7964312|FD-Score:-4.25|P-value:9.79E-7 ID:SGTC_2295|Compound:7971645|FD-Score:5.51|P-value:5.67E-10 ID:SGTC_2300|Compound:7976697|FD-Score:-3.83|P-value:8.88E-6 ID:SGTC_2359|Compound:9039622|FD-Score:-3.30|P-value:1.07E-4 ID:SGTC_2361|Compound:9034121|FD-Score:3.29|P-value:1.43E-4 ID:SGTC_2401|Compound:5128683|FD-Score:-3.19|P-value:1.71E-4 ID:SGTC_2414|Compound:st077232|FD-Score:-3.22|P-value:1.51E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:2.91|P-value:6.90E-4 ID:SGTC_2783|Compound:7738165|FD-Score:2.90|P-value:6.99E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-2.76|P-value:9.82E-4 ID:SGTC_3196|Compound:9111891|FD-Score:-3.10|P-value:2.52E-4 ID:SGTC_3313|Compound:9133968|FD-Score:2.99|P-value:4.96E-4 ID:SGTC_3315|Compound:9136395|FD-Score:3.78|P-value:1.53E-5 ID:SGTC_86|Compound:0417-1630|FD-Score:-3.94|P-value:5.24E-6 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.58|P-value:2.25E-10 ID:SGTC_206|Compound:4546-0025|FD-Score:2.81|P-value:9.93E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-6.14|P-value:3.65E-12 ID:SGTC_697|Compound:0508-1955|FD-Score:2.87|P-value:7.85E-4 ID:SGTC_741|Compound:4582-0002|FD-Score:-2.79|P-value:8.71E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:3.13|P-value:2.79E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:-3.13|P-value:2.24E-4 ID:SGTC_1224|Compound:0823-0416|FD-Score:-2.76|P-value:9.70E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:-3.07|P-value:2.87E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:3.76|P-value:1.68E-5 ID:SGTC_1276|Compound:0871-0068|FD-Score:-3.32|P-value:9.92E-5 ID:SGTC_1379|Compound:2144-0755|FD-Score:3.31|P-value:1.30E-4 ID:SGTC_1388|Compound:3346-4487|FD-Score:3.45|P-value:7.04E-5 ID:SGTC_1524|Compound:corynanthine|FD-Score:-15.60|P-value:1.40E-67 ID:SGTC_1526|Compound:st051518|FD-Score:7.26|P-value:4.36E-16 ID:SGTC_1788|Compound:fluconazole|FD-Score:6.61|P-value:1.31E-13 ID:SGTC_1914|Compound:st062367|FD-Score:-3.09|P-value:2.61E-4 ID:SGTC_1932|Compound:st074806|FD-Score:-2.84|P-value:7.27E-4 ID:SGTC_1968|Compound:st069590|FD-Score:3.23|P-value:1.89E-4 ID:SGTC_1991|Compound:st073000|FD-Score:3.26|P-value:1.65E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-3.12|P-value:2.35E-4 ID:SGTC_2094|Compound:5332512|FD-Score:3.87|P-value:9.60E-6 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.74|P-value:1.82E-5 ID:SGTC_2135|Compound:5326629|FD-Score:-3.50|P-value:4.47E-5 ID:SGTC_2247|Compound:cerulenin|FD-Score:-3.00|P-value:3.84E-4 ID:SGTC_2260|Compound:7964312|FD-Score:-4.25|P-value:9.79E-7 ID:SGTC_2295|Compound:7971645|FD-Score:5.51|P-value:5.67E-10 ID:SGTC_2300|Compound:7976697|FD-Score:-3.83|P-value:8.88E-6 ID:SGTC_2359|Compound:9039622|FD-Score:-3.30|P-value:1.07E-4 ID:SGTC_2361|Compound:9034121|FD-Score:3.29|P-value:1.43E-4 ID:SGTC_2401|Compound:5128683|FD-Score:-3.19|P-value:1.71E-4 ID:SGTC_2414|Compound:st077232|FD-Score:-3.22|P-value:1.51E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:2.91|P-value:6.90E-4 ID:SGTC_2783|Compound:7738165|FD-Score:2.90|P-value:6.99E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-2.76|P-value:9.82E-4 ID:SGTC_3196|Compound:9111891|FD-Score:-3.10|P-value:2.52E-4 ID:SGTC_3313|Compound:9133968|FD-Score:2.99|P-value:4.96E-4 ID:SGTC_3315|Compound:9136395|FD-Score:3.78|P-value:1.53E-5

Top fitness defect scores for YOL034W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1526 st051518 7.26 75.0 µM 4.36E-16 1 1.88E-13 1.74
2 SGTC_1788 fluconazole 6.61 20.0 µM 1.31E-13 azole & statin 3 1.98E-11 1.22
3 SGTC_2295 7971645 5.51 200.0 µM 5.67E-10 3 1.84E-8 1.11
4 SGTC_2094 5332512 3.87 191.7 µM 9.60E-6 5 5.38E-5 0.17
5 SGTC_3315 9136395 3.78 68.5 µM 1.53E-5 10 7.91E-5 0.04
6 SGTC_1271 0832-2808 3.76 169.0 µM 1.68E-5 7 8.56E-5 0.01
7 SGTC_2130 NSC 7 3.74 2.5 µM 1.82E-5 copper-dependent oxidative stress 5 9.12E-5 0.47
8 SGTC_1388 3346-4487 3.45 254.0 µM 7.04E-5 3 2.78E-4 0.09
9 SGTC_1379 2144-0755 3.31 36.5 µM 1.30E-4 11 4.62E-4 0.18
10 SGTC_2361 9034121 3.29 116.0 µM 1.43E-4 RSC complex & mRNA processing 26 4.98E-4 0.00
11 SGTC_1991 st073000 3.26 8.9 µM 1.65E-4 NEO1 27 5.63E-4 0.05
12 SGTC_1968 st069590 3.23 43.6 µM 1.89E-4 22 6.28E-4 0.02
13 SGTC_937 3122-0005 3.13 46.7 µM 2.79E-4 11 8.66E-4 0.12
14 SGTC_3313 9133968 2.99 57.0 µM 4.96E-4 7 0.00139 0.19
15 SGTC_2612 dictamnine 2.91 15.5 µM 6.90E-4 RNA pol III & RNase P/MRP 50 0.00183 0.05
16 SGTC_2783 7738165 2.90 71.4 µM 6.99E-4 19 0.00185 0.05
17 SGTC_697 0508-1955 2.87 564.0 µM 7.85E-4 9 0.00204 0.11
18 SGTC_206 4546-0025 2.81 72.0 µM 9.93E-4 NEO1 20 0.00248 4.13E-4
19 SGTC_1229 0139-0193 2.80 12.2 µM 0.00103 RSC complex & mRNA processing 37 0.00255 0.01
20 SGTC_1813 st051246 2.75 8.6 µM 0.00123 TSC3-RPN4 10 0.00297 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.138 8.35E-16 YDL120W YFH1 het Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia
0.130 3.73E-14 YML060W OGG1 hom Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance
0.125 3.57E-13 YJL202C_d YJL202C_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex
0.124 6.83E-13 YEL071W DLD3 hom D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm
0.120 2.62E-12 YKL099C UTP11 het Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
0.119 3.84E-12 YER159C BUR6 het Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
0.116 1.97E-11 YER171W RAD3 het 5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress
0.115 2.13E-11 YDL118W_d YDL118W_d hom Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein
0.108 4.04E-10 YLL011W SOF1 het Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
0.108 4.20E-10 YIL083C CAB2 het Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
0.106 7.67E-10 YNL338W_d YNL338W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV
0.106 8.47E-10 YIL078W THS1 het Threonyl-tRNA synthetase, essential cytoplasmic protein
0.104 1.31E-9 YJL116C NCA3 hom Protein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication
0.103 2.04E-9 YML065W ORC1 het Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity
0.103 2.43E-9 YGR245C SDA1 het Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis