YOL035C_d

Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL035C / YOL035C

Click on Significant Values for Screen Details ID:SGTC_19|Compound:0279-0214|FD-Score:3.11|P-value:7.32E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.17|P-value:1.02E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:3.53|P-value:1.53E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.48|P-value:1.84E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.57|P-value:1.31E-4 ID:SGTC_428|Compound:3448-4153|FD-Score:4.02|P-value:1.98E-5 ID:SGTC_442|Compound:cape|FD-Score:3.59|P-value:1.22E-4 ID:SGTC_585|Compound:0kpi-0023|FD-Score:3.06|P-value:8.69E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:-3.20|P-value:6.10E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:3.16|P-value:6.05E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:3.33|P-value:3.25E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:-3.94|P-value:3.30E-5 ID:SGTC_884|Compound:0851-0618|FD-Score:4.09|P-value:1.49E-5 ID:SGTC_891|Compound:0929-0073|FD-Score:5.10|P-value:9.50E-8 ID:SGTC_996|Compound:3970-0799|FD-Score:3.10|P-value:7.66E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:4.16|P-value:1.09E-5 ID:SGTC_1109|Compound:tnp00067|FD-Score:-3.69|P-value:9.57E-5 ID:SGTC_1369|Compound:1866-0067|FD-Score:-3.08|P-value:9.33E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:-3.06|P-value:9.86E-4 ID:SGTC_1443|Compound:4112-3303|FD-Score:3.09|P-value:7.86E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:3.28|P-value:4.00E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.07|P-value:8.29E-4 ID:SGTC_1646|Compound:st011709|FD-Score:-3.10|P-value:8.65E-4 ID:SGTC_1781|Compound:st038725|FD-Score:-3.49|P-value:2.11E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:3.63|P-value:1.05E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:4.88|P-value:3.22E-7 ID:SGTC_1945|Compound:st075991|FD-Score:3.26|P-value:4.36E-4 ID:SGTC_2016|Compound:5121758|FD-Score:4.00|P-value:2.23E-5 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:4.09|P-value:1.45E-5 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-3.51|P-value:1.93E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:-3.79|P-value:6.30E-5 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.12|P-value:7.02E-4 ID:SGTC_2924|Compound:9010168|FD-Score:-4.41|P-value:3.97E-6 ID:SGTC_3244|Compound:9134489|FD-Score:4.43|P-value:2.98E-6 ID:SGTC_3349|Compound:9149703|FD-Score:3.13|P-value:6.91E-4 ID:SGTC_19|Compound:0279-0214|FD-Score:3.11|P-value:7.32E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.17|P-value:1.02E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:3.53|P-value:1.53E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.48|P-value:1.84E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.57|P-value:1.31E-4 ID:SGTC_428|Compound:3448-4153|FD-Score:4.02|P-value:1.98E-5 ID:SGTC_442|Compound:cape|FD-Score:3.59|P-value:1.22E-4 ID:SGTC_585|Compound:0kpi-0023|FD-Score:3.06|P-value:8.69E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:-3.20|P-value:6.10E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:3.16|P-value:6.05E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:3.33|P-value:3.25E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:-3.94|P-value:3.30E-5 ID:SGTC_884|Compound:0851-0618|FD-Score:4.09|P-value:1.49E-5 ID:SGTC_891|Compound:0929-0073|FD-Score:5.10|P-value:9.50E-8 ID:SGTC_996|Compound:3970-0799|FD-Score:3.10|P-value:7.66E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:4.16|P-value:1.09E-5 ID:SGTC_1109|Compound:tnp00067|FD-Score:-3.69|P-value:9.57E-5 ID:SGTC_1369|Compound:1866-0067|FD-Score:-3.08|P-value:9.33E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:-3.06|P-value:9.86E-4 ID:SGTC_1443|Compound:4112-3303|FD-Score:3.09|P-value:7.86E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:3.28|P-value:4.00E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.07|P-value:8.29E-4 ID:SGTC_1646|Compound:st011709|FD-Score:-3.10|P-value:8.65E-4 ID:SGTC_1781|Compound:st038725|FD-Score:-3.49|P-value:2.11E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:3.63|P-value:1.05E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:4.88|P-value:3.22E-7 ID:SGTC_1945|Compound:st075991|FD-Score:3.26|P-value:4.36E-4 ID:SGTC_2016|Compound:5121758|FD-Score:4.00|P-value:2.23E-5 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:4.09|P-value:1.45E-5 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-3.51|P-value:1.93E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:-3.79|P-value:6.30E-5 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.12|P-value:7.02E-4 ID:SGTC_2924|Compound:9010168|FD-Score:-4.41|P-value:3.97E-6 ID:SGTC_3244|Compound:9134489|FD-Score:4.43|P-value:2.98E-6 ID:SGTC_3349|Compound:9149703|FD-Score:3.13|P-value:6.91E-4

Top fitness defect scores for YOL035C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_891 0929-0073 5.10 133.0 µM 9.50E-8 4 1.67E-7
2 SGTC_1926 methyl fluorone black 4.88 53.6 µM 3.22E-7 DNA intercalators 37 5.41E-7
3 SGTC_3244 9134489 4.43 49.5 µM 2.98E-6 11 4.62E-6
4 SGTC_58 0957-0393 4.17 9.0 µM 1.02E-5 cell wall 358 1.51E-5
5 SGTC_1107 amlodipine 4.16 47.0 µM 1.09E-5 27 1.62E-5
6 SGTC_2571 chrysanthemic acid 4.09 100.0 µM 1.45E-5 14 2.13E-5
7 SGTC_884 0851-0618 4.09 334.0 µM 1.49E-5 14 2.18E-5
8 SGTC_428 3448-4153 4.02 16.5 µM 1.98E-5 TSC3-RPN4 28 2.88E-5
9 SGTC_2016 5121758 4.00 140.0 µM 2.23E-5 redox potentiating 50 3.22E-5
10 SGTC_1789 tunicamycin 3.63 25.0 nM 1.05E-4 unfolded protein response 62 1.44E-4
11 SGTC_442 cape 3.59 100.0 µM 1.22E-4 22 1.67E-4
12 SGTC_256 clotrimazole 3.57 625.0 nM 1.31E-4 ergosterol depletion effects on membrane 37 1.78E-4
13 SGTC_145 3737-0113 3.53 38.4 µM 1.53E-4 DNA intercalators 95 2.07E-4
14 SGTC_238 5hr heat shock (37°C) + nsc-638432 3.48 8.9 µM 1.84E-4 heat shock/prefoldin 57 2.47E-4
15 SGTC_873 0591-4978 3.33 488.0 µM 3.25E-4 30 4.27E-4
16 SGTC_1493 4348-0038 3.28 111.0 µM 4.00E-4 RSC complex & mRNA processing 53 5.22E-4
17 SGTC_1945 st075991 3.26 56.6 µM 4.36E-4 60S ribosome export 43 5.67E-4
18 SGTC_872 0588-0139 3.16 234.0 µM 6.05E-4 61 7.77E-4
19 SGTC_3349 9149703 3.13 23.0 µM 6.91E-4 39 8.83E-4
20 SGTC_2749 bexarotene 3.12 57.4 µM 7.02E-4 61 8.98E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.164 1.38E-21 YGR070W ROM1 hom GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP
0.154 2.73E-19 YER099C PRS2 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication
0.153 6.51E-19 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.152 1.06E-18 YHR149C SKG6 hom Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.151 1.47E-18 YPR059C_d YPR059C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W
0.145 2.64E-17 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.136 2.39E-15 YBR067C TIP1 hom Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
0.135 4.06E-15 YJL025W RRN7 het Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
0.133 1.10E-14 YIL144W TID3 het Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
0.132 2.05E-14 YDR515W SLF1 hom RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.131 2.44E-14 YBR182C-A_p YBR182C-A_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.129 5.51E-14 YPR104C FHL1 het Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
0.129 6.95E-14 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.123 9.47E-13 YGL234W ADE5,7 hom Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities
0.118 6.05E-12 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain