YOL038W / PRE6

Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOL038W / PRE6

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:5.99|P-value:1.04E-7 ID:SGTC_64|Compound:000l-0018|FD-Score:19.20|P-value:1.75E-62 ID:SGTC_86|Compound:0417-1630|FD-Score:3.96|P-value:3.12E-4 ID:SGTC_132|Compound:0417-1715|FD-Score:3.95|P-value:3.22E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:17.90|P-value:8.73E-55 ID:SGTC_141|Compound:0443-0274|FD-Score:-4.16|P-value:1.38E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:6.81|P-value:1.78E-9 ID:SGTC_158|Compound:k038-0087|FD-Score:5.17|P-value:3.80E-6 ID:SGTC_165|Compound:k038-0023|FD-Score:4.28|P-value:1.07E-4 ID:SGTC_283|Compound:0987-0079|FD-Score:4.46|P-value:5.67E-5 ID:SGTC_288|Compound:1315-0019|FD-Score:-3.66|P-value:6.83E-4 ID:SGTC_293|Compound:4049-0273|FD-Score:-3.94|P-value:2.80E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:5.72|P-value:3.66E-7 ID:SGTC_307|Compound:1501-1223|FD-Score:4.52|P-value:4.63E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:4.22|P-value:1.33E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:4.08|P-value:2.10E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:-4.94|P-value:7.80E-6 ID:SGTC_366|Compound:0181-0206|FD-Score:3.82|P-value:4.86E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:13.40|P-value:1.26E-31 ID:SGTC_371|Compound:0195-0006|FD-Score:4.78|P-value:1.78E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-9.75|P-value:9.38E-18 ID:SGTC_444|Compound:curcumin|FD-Score:4.95|P-value:9.06E-6 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.27|P-value:1.12E-4 ID:SGTC_500|Compound:h-89|FD-Score:4.21|P-value:1.36E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:-3.92|P-value:3.00E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:3.71|P-value:6.66E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.36|P-value:6.77E-5 ID:SGTC_581|Compound:0958-0271|FD-Score:7.27|P-value:1.44E-10 ID:SGTC_584|Compound:0986-0246|FD-Score:3.76|P-value:5.76E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:4.32|P-value:9.34E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:3.73|P-value:6.37E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:3.64|P-value:8.33E-4 ID:SGTC_743|Compound:1275-0778|FD-Score:-3.69|P-value:6.24E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:-3.72|P-value:5.72E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:3.63|P-value:8.66E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:4.23|P-value:1.26E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:4.17|P-value:1.58E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:4.75|P-value:1.97E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.94|P-value:2.85E-4 ID:SGTC_1088|Compound:bifonazole|FD-Score:3.90|P-value:3.73E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:4.68|P-value:2.55E-5 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.65|P-value:8.18E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-7.61|P-value:1.55E-11 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.64|P-value:8.39E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:-5.26|P-value:2.20E-6 ID:SGTC_1327|Compound:1436-0082|FD-Score:3.99|P-value:2.86E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-4.72|P-value:1.82E-5 ID:SGTC_1353|Compound:1498-1206|FD-Score:-4.33|P-value:7.64E-5 ID:SGTC_1422|Compound:4031-0024|FD-Score:4.57|P-value:3.83E-5 ID:SGTC_1456|Compound:k015-0012|FD-Score:3.69|P-value:7.13E-4 ID:SGTC_1508|Compound:1080-0625|FD-Score:3.84|P-value:4.50E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:16.80|P-value:1.22E-48 ID:SGTC_1611|Compound:st001856|FD-Score:4.14|P-value:1.74E-4 ID:SGTC_1621|Compound:st003705|FD-Score:3.89|P-value:3.91E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.19|P-value:1.45E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-8.07|P-value:9.80E-13 ID:SGTC_1750|Compound:st081859|FD-Score:4.63|P-value:3.07E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-4.91|P-value:8.88E-6 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.78|P-value:4.71E-4 ID:SGTC_1814|Compound:st052110|FD-Score:3.70|P-value:7.01E-4 ID:SGTC_1994|Compound:st072947|FD-Score:3.89|P-value:3.86E-4 ID:SGTC_1997|Compound:st073024|FD-Score:4.17|P-value:1.55E-4 ID:SGTC_1999|Compound:st074831|FD-Score:-4.26|P-value:9.63E-5 ID:SGTC_2107|Compound:5363541|FD-Score:4.06|P-value:2.28E-4 ID:SGTC_2155|Compound:5570423|FD-Score:-3.75|P-value:5.16E-4 ID:SGTC_2202|Compound:7226349|FD-Score:-4.75|P-value:1.66E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.06|P-value:2.22E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:7.43|P-value:5.98E-11 ID:SGTC_2275|Compound:7947463|FD-Score:3.74|P-value:6.15E-4 ID:SGTC_2383|Compound:9036125|FD-Score:-3.98|P-value:2.49E-4 ID:SGTC_2384|Compound:9036245|FD-Score:3.82|P-value:4.86E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.68|P-value:2.56E-5 ID:SGTC_2708|Compound:st078559|FD-Score:3.86|P-value:4.22E-4 ID:SGTC_2711|Compound:st079103|FD-Score:3.83|P-value:4.63E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-6.33|P-value:1.63E-8 ID:SGTC_2784|Compound:7567673|FD-Score:4.35|P-value:8.42E-5 ID:SGTC_2788|Compound:5282734|FD-Score:3.69|P-value:7.11E-4 ID:SGTC_2789|Compound:5281112|FD-Score:5.37|P-value:1.67E-6 ID:SGTC_2791|Compound:5663188|FD-Score:-3.81|P-value:4.27E-4 ID:SGTC_2796|Compound:7949634|FD-Score:3.85|P-value:4.40E-4 ID:SGTC_2921|Compound:7974240|FD-Score:3.91|P-value:3.58E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-4.03|P-value:2.15E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.80|P-value:4.40E-4 ID:SGTC_3142|Compound:9086498|FD-Score:4.31|P-value:9.67E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:5.99|P-value:1.04E-7 ID:SGTC_64|Compound:000l-0018|FD-Score:19.20|P-value:1.75E-62 ID:SGTC_86|Compound:0417-1630|FD-Score:3.96|P-value:3.12E-4 ID:SGTC_132|Compound:0417-1715|FD-Score:3.95|P-value:3.22E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:17.90|P-value:8.73E-55 ID:SGTC_141|Compound:0443-0274|FD-Score:-4.16|P-value:1.38E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:6.81|P-value:1.78E-9 ID:SGTC_158|Compound:k038-0087|FD-Score:5.17|P-value:3.80E-6 ID:SGTC_165|Compound:k038-0023|FD-Score:4.28|P-value:1.07E-4 ID:SGTC_283|Compound:0987-0079|FD-Score:4.46|P-value:5.67E-5 ID:SGTC_288|Compound:1315-0019|FD-Score:-3.66|P-value:6.83E-4 ID:SGTC_293|Compound:4049-0273|FD-Score:-3.94|P-value:2.80E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:5.72|P-value:3.66E-7 ID:SGTC_307|Compound:1501-1223|FD-Score:4.52|P-value:4.63E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:4.22|P-value:1.33E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:4.08|P-value:2.10E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:-4.94|P-value:7.80E-6 ID:SGTC_366|Compound:0181-0206|FD-Score:3.82|P-value:4.86E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:13.40|P-value:1.26E-31 ID:SGTC_371|Compound:0195-0006|FD-Score:4.78|P-value:1.78E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-9.75|P-value:9.38E-18 ID:SGTC_444|Compound:curcumin|FD-Score:4.95|P-value:9.06E-6 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.27|P-value:1.12E-4 ID:SGTC_500|Compound:h-89|FD-Score:4.21|P-value:1.36E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:-3.92|P-value:3.00E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:3.71|P-value:6.66E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.36|P-value:6.77E-5 ID:SGTC_581|Compound:0958-0271|FD-Score:7.27|P-value:1.44E-10 ID:SGTC_584|Compound:0986-0246|FD-Score:3.76|P-value:5.76E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:4.32|P-value:9.34E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:3.73|P-value:6.37E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:3.64|P-value:8.33E-4 ID:SGTC_743|Compound:1275-0778|FD-Score:-3.69|P-value:6.24E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:-3.72|P-value:5.72E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:3.63|P-value:8.66E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:4.23|P-value:1.26E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:4.17|P-value:1.58E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:4.75|P-value:1.97E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.94|P-value:2.85E-4 ID:SGTC_1088|Compound:bifonazole|FD-Score:3.90|P-value:3.73E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:4.68|P-value:2.55E-5 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.65|P-value:8.18E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-7.61|P-value:1.55E-11 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.64|P-value:8.39E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:-5.26|P-value:2.20E-6 ID:SGTC_1327|Compound:1436-0082|FD-Score:3.99|P-value:2.86E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-4.72|P-value:1.82E-5 ID:SGTC_1353|Compound:1498-1206|FD-Score:-4.33|P-value:7.64E-5 ID:SGTC_1422|Compound:4031-0024|FD-Score:4.57|P-value:3.83E-5 ID:SGTC_1456|Compound:k015-0012|FD-Score:3.69|P-value:7.13E-4 ID:SGTC_1508|Compound:1080-0625|FD-Score:3.84|P-value:4.50E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:16.80|P-value:1.22E-48 ID:SGTC_1611|Compound:st001856|FD-Score:4.14|P-value:1.74E-4 ID:SGTC_1621|Compound:st003705|FD-Score:3.89|P-value:3.91E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.19|P-value:1.45E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-8.07|P-value:9.80E-13 ID:SGTC_1750|Compound:st081859|FD-Score:4.63|P-value:3.07E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-4.91|P-value:8.88E-6 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.78|P-value:4.71E-4 ID:SGTC_1814|Compound:st052110|FD-Score:3.70|P-value:7.01E-4 ID:SGTC_1994|Compound:st072947|FD-Score:3.89|P-value:3.86E-4 ID:SGTC_1997|Compound:st073024|FD-Score:4.17|P-value:1.55E-4 ID:SGTC_1999|Compound:st074831|FD-Score:-4.26|P-value:9.63E-5 ID:SGTC_2107|Compound:5363541|FD-Score:4.06|P-value:2.28E-4 ID:SGTC_2155|Compound:5570423|FD-Score:-3.75|P-value:5.16E-4 ID:SGTC_2202|Compound:7226349|FD-Score:-4.75|P-value:1.66E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.06|P-value:2.22E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:7.43|P-value:5.98E-11 ID:SGTC_2275|Compound:7947463|FD-Score:3.74|P-value:6.15E-4 ID:SGTC_2383|Compound:9036125|FD-Score:-3.98|P-value:2.49E-4 ID:SGTC_2384|Compound:9036245|FD-Score:3.82|P-value:4.86E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.68|P-value:2.56E-5 ID:SGTC_2708|Compound:st078559|FD-Score:3.86|P-value:4.22E-4 ID:SGTC_2711|Compound:st079103|FD-Score:3.83|P-value:4.63E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-6.33|P-value:1.63E-8 ID:SGTC_2784|Compound:7567673|FD-Score:4.35|P-value:8.42E-5 ID:SGTC_2788|Compound:5282734|FD-Score:3.69|P-value:7.11E-4 ID:SGTC_2789|Compound:5281112|FD-Score:5.37|P-value:1.67E-6 ID:SGTC_2791|Compound:5663188|FD-Score:-3.81|P-value:4.27E-4 ID:SGTC_2796|Compound:7949634|FD-Score:3.85|P-value:4.40E-4 ID:SGTC_2921|Compound:7974240|FD-Score:3.91|P-value:3.58E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-4.03|P-value:2.15E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.80|P-value:4.40E-4 ID:SGTC_3142|Compound:9086498|FD-Score:4.31|P-value:9.67E-5

Top fitness defect scores for YOL038W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_64 000l-0018 19.20 272.1 µM 1.75E-62 ubiquinone biosynthesis & proteasome 5 3.90E-82 2.27
2 SGTC_137 0438-0332 17.90 85.4 µM 8.73E-55 ubiquinone biosynthesis & proteasome 3 5.96E-72 1.39
3 SGTC_1597 7-benzyloxygramine 16.80 47.5 µM 1.22E-48 calcium & mitochondrial duress 5 8.07E-64 1.33
4 SGTC_367 0335-0881 13.40 7.1 µM 1.26E-31 ubiquinone biosynthesis & proteasome 1 2.58E-41 2.35
5 SGTC_2250 alverine citrate 7.43 130.0 µM 5.98E-11 15 5.44E-14 0.18
6 SGTC_581 0958-0271 7.27 211.0 µM 1.44E-10 1 1.74E-13 2.91
7 SGTC_142 0335-0849 6.81 62.7 µM 1.78E-9 ubiquinone biosynthesis & proteasome 9 4.78E-12 1.22
8 SGTC_36 0335-0881 5.99 14.2 µM 1.04E-7 ubiquinone biosynthesis & proteasome 15 1.02E-9 0.55
9 SGTC_294 k038-0071 5.72 157.0 µM 3.66E-7 ubiquinone biosynthesis & proteasome 7 5.35E-9 0.16
10 SGTC_2789 5281112 5.37 71.4 µM 1.67E-6 3 3.93E-8 0.82
11 SGTC_158 k038-0087 5.17 26.4 µM 3.80E-6 ubiquinone biosynthesis & proteasome 18 1.16E-7 0.04
12 SGTC_444 curcumin 4.95 90.4 µM 9.06E-6 iron homeostasis 6 3.64E-7 0.28
13 SGTC_371 0195-0006 4.78 7.4 µM 1.78E-5 7 8.86E-7 0.21
14 SGTC_1019 4262-0212 4.75 3.4 µM 1.97E-5 ergosterol depletion effects on membrane 9 1.01E-6 0.05
15 SGTC_1119 parthenolide 4.68 318.5 nM 2.55E-5 TSC3-RPN4 6 1.41E-6 0.19
16 SGTC_2688 kanamycin b 4.68 96.7 µM 2.56E-5 calcium & mitochondrial duress 10 1.42E-6 0.03
17 SGTC_1750 st081859 4.63 2.4 µM 3.07E-5 endomembrane recycling 25 1.81E-6 0.07
18 SGTC_1422 4031-0024 4.57 15.5 µM 3.83E-5 iron homeostasis 7 2.42E-6 0.15
19 SGTC_307 1501-1223 4.52 22.3 µM 4.63E-5 3 3.10E-6 0.03
20 SGTC_283 0987-0079 4.46 11.7 µM 5.67E-5 3 4.04E-6 0.23

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.534 1.07E-246 YFR050C PRE4 het Beta 7 subunit of the 20S proteasome
0.527 1.06E-238 YMR314W PRE5 het Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.490 4.75E-202 YER012W PRE1 het Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
0.479 6.74E-192 YER094C PUP3 het Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
0.455 5.00E-171 YOR362C PRE10 het Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.427 1.34E-148 YGL011C SCL1 het Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
0.408 1.10E-134 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.383 1.18E-117 YGR253C PUP2 het Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.381 3.36E-116 YML092C PRE8 het Alpha 2 subunit of the 20S proteasome
0.359 1.76E-102 YLR021W IRC25 hom Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.342 8.27E-93 YPR146C_d YPR146C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.327 1.48E-84 YJL001W PRE3 het Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
0.324 1.50E-82 YJL193W_p YJL193W_p hom Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
0.320 5.94E-81 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.317 3.08E-79 YML034C-A_d YML034C-A_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data