YOL042W / NGL1

Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL042W / NGL1

Click on Significant Values for Screen Details ID:SGTC_44|Compound:0929-0055|FD-Score:3.11|P-value:6.59E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:-3.42|P-value:2.14E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:5.32|P-value:2.05E-8 ID:SGTC_96|Compound:0929-0093|FD-Score:3.83|P-value:3.82E-5 ID:SGTC_333|Compound:3486-0131|FD-Score:3.15|P-value:5.79E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:4.06|P-value:1.40E-5 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.14|P-value:6.19E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.10|P-value:6.92E-4 ID:SGTC_494|Compound:Boc-Gly-Val-Val-CHO|FD-Score:-3.55|P-value:1.28E-4 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:3.66|P-value:7.80E-5 ID:SGTC_535|Compound:2293-4129|FD-Score:-4.37|P-value:3.41E-6 ID:SGTC_537|Compound:1334-0398|FD-Score:-3.56|P-value:1.26E-4 ID:SGTC_592|Compound:k241-0073|FD-Score:5.21|P-value:3.76E-8 ID:SGTC_723|Compound:4182-0001|FD-Score:-3.32|P-value:3.23E-4 ID:SGTC_765|Compound:1319-0117|FD-Score:-3.52|P-value:1.44E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:4.22|P-value:6.50E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:3.75|P-value:5.35E-5 ID:SGTC_929|Compound:2805-0021|FD-Score:-5.15|P-value:5.74E-8 ID:SGTC_968|Compound:1309-0633|FD-Score:3.00|P-value:9.79E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.16|P-value:5.44E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.85|P-value:3.67E-5 ID:SGTC_1720|Compound:st037298|FD-Score:6.67|P-value:2.91E-12 ID:SGTC_1845|Compound:st056753|FD-Score:3.11|P-value:6.61E-4 ID:SGTC_1917|Compound:st066507|FD-Score:-3.11|P-value:7.00E-4 ID:SGTC_2135|Compound:5326629|FD-Score:-4.05|P-value:1.56E-5 ID:SGTC_2136|Compound:5328288|FD-Score:-3.73|P-value:6.21E-5 ID:SGTC_2274|Compound:7947094|FD-Score:-3.06|P-value:8.23E-4 ID:SGTC_2304|Compound:7443016|FD-Score:-3.29|P-value:3.60E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-3.59|P-value:1.13E-4 ID:SGTC_2349|Compound:9023730|FD-Score:-3.55|P-value:1.28E-4 ID:SGTC_2518|Compound:prenyletin|FD-Score:3.11|P-value:6.70E-4 ID:SGTC_2528|Compound:xanthyletin|FD-Score:-3.01|P-value:9.95E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.88|P-value:3.12E-5 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.14|P-value:6.23E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:3.11|P-value:6.59E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:-3.42|P-value:2.14E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:5.32|P-value:2.05E-8 ID:SGTC_96|Compound:0929-0093|FD-Score:3.83|P-value:3.82E-5 ID:SGTC_333|Compound:3486-0131|FD-Score:3.15|P-value:5.79E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:4.06|P-value:1.40E-5 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.14|P-value:6.19E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.10|P-value:6.92E-4 ID:SGTC_494|Compound:Boc-Gly-Val-Val-CHO|FD-Score:-3.55|P-value:1.28E-4 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:3.66|P-value:7.80E-5 ID:SGTC_535|Compound:2293-4129|FD-Score:-4.37|P-value:3.41E-6 ID:SGTC_537|Compound:1334-0398|FD-Score:-3.56|P-value:1.26E-4 ID:SGTC_592|Compound:k241-0073|FD-Score:5.21|P-value:3.76E-8 ID:SGTC_723|Compound:4182-0001|FD-Score:-3.32|P-value:3.23E-4 ID:SGTC_765|Compound:1319-0117|FD-Score:-3.52|P-value:1.44E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:4.22|P-value:6.50E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:3.75|P-value:5.35E-5 ID:SGTC_929|Compound:2805-0021|FD-Score:-5.15|P-value:5.74E-8 ID:SGTC_968|Compound:1309-0633|FD-Score:3.00|P-value:9.79E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.16|P-value:5.44E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.85|P-value:3.67E-5 ID:SGTC_1720|Compound:st037298|FD-Score:6.67|P-value:2.91E-12 ID:SGTC_1845|Compound:st056753|FD-Score:3.11|P-value:6.61E-4 ID:SGTC_1917|Compound:st066507|FD-Score:-3.11|P-value:7.00E-4 ID:SGTC_2135|Compound:5326629|FD-Score:-4.05|P-value:1.56E-5 ID:SGTC_2136|Compound:5328288|FD-Score:-3.73|P-value:6.21E-5 ID:SGTC_2274|Compound:7947094|FD-Score:-3.06|P-value:8.23E-4 ID:SGTC_2304|Compound:7443016|FD-Score:-3.29|P-value:3.60E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-3.59|P-value:1.13E-4 ID:SGTC_2349|Compound:9023730|FD-Score:-3.55|P-value:1.28E-4 ID:SGTC_2518|Compound:prenyletin|FD-Score:3.11|P-value:6.70E-4 ID:SGTC_2528|Compound:xanthyletin|FD-Score:-3.01|P-value:9.95E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.88|P-value:3.12E-5 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.14|P-value:6.23E-4

Top fitness defect scores for YOL042W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_1720 st037298 6.67 9.5 µM 2.91E-12 9 1.25E-11
2 SGTC_56 0957-0365 5.32 83.2 µM 2.05E-8 Golgi 22 5.26E-8
3 SGTC_592 k241-0073 5.21 46.5 µM 3.76E-8 9 9.32E-8
4 SGTC_882 0843-0131 4.22 351.0 µM 6.50E-6 15 1.20E-5
5 SGTC_390 dec-rvkr-cmk 4.06 16.7 µM 1.40E-5 calcium & mitochondrial duress 20 2.47E-5
6 SGTC_2564 betulinic acid 3.88 100.0 µM 3.12E-5 31 5.25E-5
7 SGTC_96 0929-0093 3.83 37.3 µM 3.82E-5 calcium & mitochondrial duress 15 6.36E-5
8 SGTC_928 2764-0428 3.75 58.7 µM 5.35E-5 cell wall 66 8.75E-5
9 SGTC_506 n9-isopropylolomoucine 3.66 153.0 µM 7.80E-5 13 1.25E-4
10 SGTC_1061 3448-9325 3.16 110.0 µM 5.44E-4 46 7.80E-4
11 SGTC_333 3486-0131 3.15 40.1 µM 5.79E-4 amide catabolism 33 8.27E-4
12 SGTC_44 0929-0055 3.11 61.2 µM 6.59E-4 68 9.34E-4
13 SGTC_1845 st056753 3.11 3.0 µM 6.61E-4 TSC3-RPN4 68 9.36E-4
14 SGTC_2518 prenyletin 3.11 46.3 µM 6.70E-4 29 9.49E-4
15 SGTC_453 enantio-paf c16 3.10 10.0 µM 6.92E-4 231 9.78E-4
16 SGTC_968 1309-0633 3.00 340.0 µM 9.79E-4 51 0.00136
17 SGTC_2428 5615643 2.96 7.8 µM 0.00112 69 0.00154
18 SGTC_2563 tomatine 2.89 8.5 µM 0.00143 125 0.00195
19 SGTC_1630 st005209 2.83 31.3 µM 0.00173 98 0.00232
20 SGTC_917 harmaline 2.80 728.1 nM 0.00188 ergosterol biosynthesis 90 0.00252

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.147 1.25E-17 YOR190W SPR1 hom Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance
0.113 6.38E-11 YPL134C ODC1 hom Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation
0.111 1.01E-10 YDR044W HEM13 het Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p)
0.100 6.76E-9 YPL221W FLC1 hom Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance
0.095 3.29E-8 YEL017W GTT3 hom Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery
0.090 1.77E-7 YER151C UBP3 hom Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress
0.087 4.96E-7 YML101C-A_d YML101C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.084 1.25E-6 YOR076C SKI7 hom Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype
0.083 1.66E-6 YPR093C ASR1 hom Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein
0.082 1.84E-6 YOR087W YVC1 hom Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock
0.082 2.25E-6 YBR077C SLM4 hom Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4
0.080 3.42E-6 YBR168W PEX32 hom Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p
0.079 4.22E-6 YBR070C ALG14 het Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
0.079 4.31E-6 YCR022C_d YCR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene
0.078 5.85E-6 YJR140C HIR3 hom Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein