YOL048C / RRT8

Protein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL048C / RRT8

Click on Significant Values for Screen Details

Top fitness defect scores for YOL048C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_512 prima-1 5.32 270.0 µM 3.19E-8 DNA intercalators 6 5.25E-8
2 SGTC_506 n9-isopropylolomoucine 4.76 153.0 µM 6.52E-7 5 9.80E-7
3 SGTC_1410 3909-8106 4.04 77.9 µM 1.98E-5 11 2.69E-5
4 SGTC_1909 st060837 3.96 51.5 µM 2.77E-5 51 3.71E-5
5 SGTC_942 3253-1115 3.94 227.0 µM 2.99E-5 28 4.01E-5
6 SGTC_1588 farnesol 3.80 29.9 µM 5.40E-5 12 7.11E-5
7 SGTC_2527 2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone 3.79 63.6 µM 5.80E-5 51 7.62E-5
8 SGTC_2858 9025781 3.56 9.0 µM 1.45E-4 sphingolipid biosynthesis & PDR1 119 1.85E-4
9 SGTC_87 0100-0052 3.38 35.5 µM 2.90E-4 165 3.63E-4
10 SGTC_917 harmaline 3.27 728.1 nM 4.29E-4 ergosterol biosynthesis 51 5.31E-4
11 SGTC_986 1493-0483 3.25 84.0 µM 4.66E-4 34 5.75E-4
12 SGTC_383 2324-0395 3.21 7.4 µM 5.39E-4 azole & statin 71 6.63E-4
13 SGTC_8 0354-0001 3.15 70.0 µM 6.64E-4 127 8.10E-4
14 SGTC_27 0485-0001 3.14 32.6 µM 7.05E-4 RPP1 & pyrimidine depletion 15 8.59E-4
15 SGTC_390 dec-rvkr-cmk 3.06 16.7 µM 9.07E-4 calcium & mitochondrial duress 71 0.00110
16 SGTC_511 blebbistatin 3.05 171.0 µM 9.38E-4 DNA intercalators 54 0.00113
17 SGTC_1953 st076800 3.03 12.1 µM 0.00102 101 0.00123
18 SGTC_1716 st033144 3.02 30.6 µM 0.00105 51 0.00127
19 SGTC_1556 plumbagin 3.01 1.5 µM 0.00107 superoxide 44 0.00129
20 SGTC_3281 9140613 2.95 49.5 µM 0.00135 RPP1 & pyrimidine depletion 54 0.00161

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.149 3.87E-18 YHR118C ORC6 het Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p
0.144 4.66E-17 YDR169C-A_p YDR169C-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.128 1.13E-13 YPL165C SET6 hom SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability
0.122 1.27E-12 YNL067W-B_p YNL067W-B_p hom Putative protein of unknown function
0.122 1.58E-12 YDR151C CTH1 hom Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis
0.107 4.50E-10 YLR055C SPT8 hom Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters
0.103 2.00E-9 YDL065C PEX19 hom Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning
0.101 4.98E-9 YOL046C_d YOL046C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W
0.097 2.13E-8 YFR022W ROG3 hom Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication
0.095 3.38E-8 YEL007W MIT1 hom Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
0.093 5.87E-8 YKL064W MNR2 hom Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations
0.092 8.21E-8 YML030W RCF1 hom Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast
0.092 8.46E-8 YNL134C YNL134C hom Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
0.087 4.41E-7 YDR494W RSM28 hom Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation
0.084 1.15E-6 YNL304W YPT11 hom Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells