YOL061W / PRS5

5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL061W / PRS5

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.90|P-value:5.47E-5 ID:SGTC_75|Compound:0159-0059|FD-Score:3.78|P-value:8.90E-5 ID:SGTC_87|Compound:0100-0052|FD-Score:4.15|P-value:1.89E-5 ID:SGTC_112|Compound:phenothiazine|FD-Score:-3.91|P-value:4.89E-5 ID:SGTC_113|Compound:0099-0221|FD-Score:-3.25|P-value:5.98E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:3.32|P-value:4.91E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:4.18|P-value:1.67E-5 ID:SGTC_122|Compound:0236-0016|FD-Score:5.03|P-value:2.93E-7 ID:SGTC_138|Compound:0850-0259|FD-Score:-3.68|P-value:1.22E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:3.62|P-value:1.65E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:3.24|P-value:6.63E-4 ID:SGTC_1563|Compound:conessine|FD-Score:4.04|P-value:3.12E-5 ID:SGTC_1730|Compound:st033808|FD-Score:-3.60|P-value:1.63E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-3.10|P-value:9.73E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.93|P-value:4.77E-5 ID:SGTC_1860|Compound:st056228|FD-Score:-4.83|P-value:7.32E-7 ID:SGTC_1877|Compound:wortmannin|FD-Score:3.30|P-value:5.42E-4 ID:SGTC_1950|Compound:st077106|FD-Score:5.37|P-value:4.88E-8 ID:SGTC_2028|Compound:5153890|FD-Score:3.38|P-value:3.96E-4 ID:SGTC_2029|Compound:5175081|FD-Score:3.59|P-value:1.88E-4 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:-3.96|P-value:3.87E-5 ID:SGTC_2066|Compound:5222451|FD-Score:-3.14|P-value:8.63E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.46|P-value:3.03E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:-4.06|P-value:2.61E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.98|P-value:3.88E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.53|P-value:2.14E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:-4.36|P-value:7.02E-6 ID:SGTC_2832|Compound:7992762|FD-Score:4.09|P-value:2.45E-5 ID:SGTC_2888|Compound:9058547|FD-Score:-3.83|P-value:6.70E-5 ID:SGTC_2947|Compound:9072639|FD-Score:3.46|P-value:3.00E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.90|P-value:5.47E-5 ID:SGTC_75|Compound:0159-0059|FD-Score:3.78|P-value:8.90E-5 ID:SGTC_87|Compound:0100-0052|FD-Score:4.15|P-value:1.89E-5 ID:SGTC_112|Compound:phenothiazine|FD-Score:-3.91|P-value:4.89E-5 ID:SGTC_113|Compound:0099-0221|FD-Score:-3.25|P-value:5.98E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:3.32|P-value:4.91E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:4.18|P-value:1.67E-5 ID:SGTC_122|Compound:0236-0016|FD-Score:5.03|P-value:2.93E-7 ID:SGTC_138|Compound:0850-0259|FD-Score:-3.68|P-value:1.22E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:3.62|P-value:1.65E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:3.24|P-value:6.63E-4 ID:SGTC_1563|Compound:conessine|FD-Score:4.04|P-value:3.12E-5 ID:SGTC_1730|Compound:st033808|FD-Score:-3.60|P-value:1.63E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-3.10|P-value:9.73E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.93|P-value:4.77E-5 ID:SGTC_1860|Compound:st056228|FD-Score:-4.83|P-value:7.32E-7 ID:SGTC_1877|Compound:wortmannin|FD-Score:3.30|P-value:5.42E-4 ID:SGTC_1950|Compound:st077106|FD-Score:5.37|P-value:4.88E-8 ID:SGTC_2028|Compound:5153890|FD-Score:3.38|P-value:3.96E-4 ID:SGTC_2029|Compound:5175081|FD-Score:3.59|P-value:1.88E-4 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:-3.96|P-value:3.87E-5 ID:SGTC_2066|Compound:5222451|FD-Score:-3.14|P-value:8.63E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.46|P-value:3.03E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:-4.06|P-value:2.61E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.98|P-value:3.88E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.53|P-value:2.14E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:-4.36|P-value:7.02E-6 ID:SGTC_2832|Compound:7992762|FD-Score:4.09|P-value:2.45E-5 ID:SGTC_2888|Compound:9058547|FD-Score:-3.83|P-value:6.70E-5 ID:SGTC_2947|Compound:9072639|FD-Score:3.46|P-value:3.00E-4

Top fitness defect scores for YOL061W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1950 st077106 5.37 7.2 µM 4.88E-8 RNA processing & uracil transport 8 3.91E-8
2 SGTC_122 0236-0016 5.03 402.4 µM 2.93E-7 RNA processing & uracil transport 15 2.40E-7
3 SGTC_120 rhodamine 6G 4.18 52.3 µM 1.67E-5 mitochondrial processes 25 1.44E-5
4 SGTC_87 0100-0052 4.15 35.5 µM 1.89E-5 97 1.63E-5
5 SGTC_2832 7992762 4.09 52.0 µM 2.45E-5 25 2.13E-5
6 SGTC_1563 conessine 4.04 56.1 µM 3.12E-5 15 2.71E-5
7 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.98 3.7 µM 3.88E-5 81 3.39E-5
8 SGTC_1844 rapamycin 3.93 4.0 nM 4.77E-5 159 4.17E-5
9 SGTC_6 0844-0013 3.90 19.8 µM 5.47E-5 94 4.80E-5
10 SGTC_75 0159-0059 3.78 199.0 µM 8.90E-5 fatty acid desaturase (OLE1) 24 7.85E-5
11 SGTC_552 k242-0005 3.62 476.0 µM 1.65E-4 25 1.47E-4
12 SGTC_2029 5175081 3.59 200.0 µM 1.88E-4 33 1.67E-4
13 SGTC_2947 9072639 3.46 7.7 µM 3.00E-4 tubulin folding & SWR complex 66 2.69E-4
14 SGTC_2419 paclitaxel 3.46 166.5 µM 3.03E-4 69 2.72E-4
15 SGTC_2028 5153890 3.38 36.4 µM 3.96E-4 cell wall signaling 49 3.56E-4
16 SGTC_114 0364-0001 3.32 490.3 µM 4.91E-4 80 4.43E-4
17 SGTC_1877 wortmannin 3.30 25.0 µM 5.42E-4 122 4.89E-4
18 SGTC_848 0368-0089 3.24 85.6 µM 6.63E-4 iron homeostasis 34 6.00E-4
19 SGTC_545 4218-0007 3.08 221.0 µM 0.00113 63 0.00103
20 SGTC_1363 1636-0063 3.07 10.4 µM 0.00118 Golgi 70 0.00108

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.147 1.41E-17 YDR306C_p YDR306C_p hom F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain
0.135 3.44E-15 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.131 2.98E-14 YDR068W DOS2 hom Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.131 3.03E-14 YDL018C ERP3 hom Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.130 3.84E-14 YDL009C_p YDL009C_p hom Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene
0.127 1.60E-13 YGL017W ATE1 hom Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
0.123 1.01E-12 YMR080C NAM7 hom ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress
0.122 1.29E-12 YDR530C APA2 hom Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication
0.120 2.85E-12 YOR274W MOD5 hom Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis
0.116 1.67E-11 YDR085C AFR1 hom Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication
0.116 1.89E-11 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.115 2.09E-11 YGR067C_p YGR067C_p hom Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p
0.115 2.78E-11 YLR026C SED5 het cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
0.112 6.59E-11 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.111 1.30E-10 YJR035W RAD26 hom Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein