YOL064C / MET22

Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL064C / MET22

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-4.12|P-value:1.99E-4 ID:SGTC_263|Compound:alizarin|FD-Score:5.11|P-value:4.03E-6 ID:SGTC_293|Compound:4049-0273|FD-Score:-3.86|P-value:4.47E-4 ID:SGTC_316|Compound:k060-0003|FD-Score:4.31|P-value:8.09E-5 ID:SGTC_318|Compound:1488-0629|FD-Score:3.63|P-value:7.38E-4 ID:SGTC_326|Compound:k292-0812|FD-Score:-3.69|P-value:7.64E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:-3.68|P-value:7.87E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:-3.74|P-value:6.58E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.53|P-value:9.86E-4 ID:SGTC_719|Compound:2810-4230|FD-Score:-4.07|P-value:2.30E-4 ID:SGTC_728|Compound:3030-5151|FD-Score:5.21|P-value:2.71E-6 ID:SGTC_730|Compound:1436-0052|FD-Score:3.87|P-value:3.50E-4 ID:SGTC_732|Compound:3970-1727|FD-Score:3.71|P-value:5.70E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:3.95|P-value:2.70E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:3.61|P-value:7.76E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:4.01|P-value:2.24E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:-3.81|P-value:5.32E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:4.15|P-value:1.40E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:4.73|P-value:1.74E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:3.81|P-value:4.23E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:4.49|P-value:4.31E-5 ID:SGTC_1369|Compound:1866-0067|FD-Score:5.02|P-value:5.75E-6 ID:SGTC_1428|Compound:4049-0204|FD-Score:4.01|P-value:2.20E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.66|P-value:6.63E-4 ID:SGTC_1728|Compound:st035614|FD-Score:-4.62|P-value:3.44E-5 ID:SGTC_1729|Compound:st035615|FD-Score:5.29|P-value:1.87E-6 ID:SGTC_1730|Compound:st033808|FD-Score:3.64|P-value:7.21E-4 ID:SGTC_1848|Compound:st056003|FD-Score:3.99|P-value:2.35E-4 ID:SGTC_1993|Compound:st073995|FD-Score:3.91|P-value:3.11E-4 ID:SGTC_2171|Compound:5739187|FD-Score:4.20|P-value:1.20E-4 ID:SGTC_2172|Compound:5754775|FD-Score:4.08|P-value:1.79E-4 ID:SGTC_2482|Compound:5981709|FD-Score:-5.41|P-value:1.57E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.04|P-value:2.56E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.96|P-value:3.29E-4 ID:SGTC_2839|Compound:9003951|FD-Score:3.98|P-value:2.50E-4 ID:SGTC_2840|Compound:9008771|FD-Score:4.49|P-value:4.25E-5 ID:SGTC_2843|Compound:9000894|FD-Score:4.57|P-value:3.20E-5 ID:SGTC_2936|Compound:9038235|FD-Score:4.16|P-value:1.37E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.12|P-value:1.99E-4 ID:SGTC_263|Compound:alizarin|FD-Score:5.11|P-value:4.03E-6 ID:SGTC_293|Compound:4049-0273|FD-Score:-3.86|P-value:4.47E-4 ID:SGTC_316|Compound:k060-0003|FD-Score:4.31|P-value:8.09E-5 ID:SGTC_318|Compound:1488-0629|FD-Score:3.63|P-value:7.38E-4 ID:SGTC_326|Compound:k292-0812|FD-Score:-3.69|P-value:7.64E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:-3.68|P-value:7.87E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:-3.74|P-value:6.58E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.53|P-value:9.86E-4 ID:SGTC_719|Compound:2810-4230|FD-Score:-4.07|P-value:2.30E-4 ID:SGTC_728|Compound:3030-5151|FD-Score:5.21|P-value:2.71E-6 ID:SGTC_730|Compound:1436-0052|FD-Score:3.87|P-value:3.50E-4 ID:SGTC_732|Compound:3970-1727|FD-Score:3.71|P-value:5.70E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:3.95|P-value:2.70E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:3.61|P-value:7.76E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:4.01|P-value:2.24E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:-3.81|P-value:5.32E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:4.15|P-value:1.40E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:4.73|P-value:1.74E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:3.81|P-value:4.23E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:4.49|P-value:4.31E-5 ID:SGTC_1369|Compound:1866-0067|FD-Score:5.02|P-value:5.75E-6 ID:SGTC_1428|Compound:4049-0204|FD-Score:4.01|P-value:2.20E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.66|P-value:6.63E-4 ID:SGTC_1728|Compound:st035614|FD-Score:-4.62|P-value:3.44E-5 ID:SGTC_1729|Compound:st035615|FD-Score:5.29|P-value:1.87E-6 ID:SGTC_1730|Compound:st033808|FD-Score:3.64|P-value:7.21E-4 ID:SGTC_1848|Compound:st056003|FD-Score:3.99|P-value:2.35E-4 ID:SGTC_1993|Compound:st073995|FD-Score:3.91|P-value:3.11E-4 ID:SGTC_2171|Compound:5739187|FD-Score:4.20|P-value:1.20E-4 ID:SGTC_2172|Compound:5754775|FD-Score:4.08|P-value:1.79E-4 ID:SGTC_2482|Compound:5981709|FD-Score:-5.41|P-value:1.57E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.04|P-value:2.56E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.96|P-value:3.29E-4 ID:SGTC_2839|Compound:9003951|FD-Score:3.98|P-value:2.50E-4 ID:SGTC_2840|Compound:9008771|FD-Score:4.49|P-value:4.25E-5 ID:SGTC_2843|Compound:9000894|FD-Score:4.57|P-value:3.20E-5 ID:SGTC_2936|Compound:9038235|FD-Score:4.16|P-value:1.37E-4

Top fitness defect scores for YOL064C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1729 st035615 5.29 46.0 µM 1.87E-6 13 5.96E-8
2 SGTC_728 3030-5151 5.21 22.5 µM 2.71E-6 5 9.70E-8
3 SGTC_263 alizarin 5.11 107.9 µM 4.03E-6 iron homeostasis 33 1.63E-7
4 SGTC_1369 1866-0067 5.02 208.0 µM 5.75E-6 RPP1 & pyrimidine depletion 20 2.59E-7
5 SGTC_1026 k007-1105 4.73 30.6 µM 1.74E-5 ERAD & cell cycle 68 1.10E-6
6 SGTC_2843 9000894 4.57 45.5 µM 3.20E-5 5 2.43E-6
7 SGTC_2840 9008771 4.49 71.4 µM 4.25E-5 6 3.51E-6
8 SGTC_1243 0416-0003 4.49 5.7 µM 4.31E-5 PDR1 14 3.57E-6
9 SGTC_316 k060-0003 4.31 58.6 µM 8.09E-5 10 8.11E-6
10 SGTC_2171 5739187 4.20 198.1 µM 1.20E-4 copper-dependent oxidative stress 13 1.35E-5
11 SGTC_2936 9038235 4.16 15.6 µM 1.37E-4 14 1.60E-5
12 SGTC_1023 4482-3888 4.15 16.0 µM 1.40E-4 TSC3-RPN4 13 1.66E-5
13 SGTC_2172 5754775 4.08 163.9 µM 1.79E-4 13 2.27E-5
14 SGTC_1428 4049-0204 4.01 61.7 µM 2.20E-4 12 2.98E-5
15 SGTC_948 3448-0295 4.01 75.3 µM 2.24E-4 26 3.04E-5
16 SGTC_1848 st056003 3.99 16.4 µM 2.35E-4 13 3.25E-5
17 SGTC_2839 9003951 3.98 26.0 µM 2.50E-4 11 3.51E-5
18 SGTC_848 0368-0089 3.95 85.6 µM 2.70E-4 iron homeostasis 13 3.88E-5
19 SGTC_1993 st073995 3.91 70.8 µM 3.11E-4 21 4.67E-5
20 SGTC_730 1436-0052 3.87 41.2 µM 3.50E-4 mitochondrial processes 16 5.44E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.143 9.52E-17 YMR182C RGM1 hom Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y-prime telomeric elements and subtelomeric COS genes; RGM1 has a paralog, USV1, that arose from the whole genome duplication
0.104 1.31E-9 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.102 3.81E-9 YEL025C_p YEL025C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
0.097 2.10E-8 YOR021C SFM1 hom SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism
0.093 5.89E-8 YDL201W TRM8 hom Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.087 4.97E-7 YDR409W SIZ1 hom SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring
0.087 5.00E-7 YIL067C_p YIL067C_p hom Uncharacterized protein of unknown function
0.080 3.24E-6 YDR255C RMD5 hom Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain
0.080 3.27E-6 YOR112W CEX1 hom Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p
0.077 8.46E-6 YMR123W PKR1 hom V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
0.076 1.15E-5 YIR018C-A_p YIR018C-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.074 1.65E-5 YKL167C MRP49 hom Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation
0.073 2.63E-5 YNL261W ORC5 het Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
0.072 2.94E-5 YAL030W SNC1 hom Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication
0.069 6.28E-5 YJL082W IML2 hom Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication