YOL066C / RIB2

Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOL066C / RIB2

Click on Significant Values for Screen Details ID:SGTC_51|Compound:1013-0126|FD-Score:4.72|P-value:1.14E-7 ID:SGTC_153|Compound:4077-0274|FD-Score:3.23|P-value:2.10E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:3.72|P-value:2.33E-5 ID:SGTC_277|Compound:nystatin|FD-Score:4.47|P-value:4.87E-7 ID:SGTC_530|Compound:4358-1714|FD-Score:3.30|P-value:1.56E-4 ID:SGTC_570|Compound:0987-0065|FD-Score:3.00|P-value:5.37E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:-3.05|P-value:3.18E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:2.84|P-value:9.66E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:3.24|P-value:2.03E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:3.01|P-value:5.16E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:5.27|P-value:3.78E-9 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-2.76|P-value:9.89E-4 ID:SGTC_1144|Compound:3486-0183|FD-Score:3.41|P-value:9.81E-5 ID:SGTC_1305|Compound:1181-0477|FD-Score:2.96|P-value:6.20E-4 ID:SGTC_1388|Compound:3346-4487|FD-Score:2.91|P-value:7.57E-4 ID:SGTC_1432|Compound:4049-0298|FD-Score:2.93|P-value:7.03E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:-3.08|P-value:2.82E-4 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.72|P-value:2.42E-5 ID:SGTC_1521|Compound:st012942|FD-Score:3.09|P-value:3.77E-4 ID:SGTC_1553|Compound:gossypol|FD-Score:3.88|P-value:1.10E-5 ID:SGTC_1608|Compound:st001473|FD-Score:2.87|P-value:8.93E-4 ID:SGTC_1618|Compound:st003135|FD-Score:3.17|P-value:2.74E-4 ID:SGTC_1634|Compound:st006576|FD-Score:3.72|P-value:2.43E-5 ID:SGTC_1742|Compound:st038235|FD-Score:3.07|P-value:3.99E-4 ID:SGTC_1756|Compound:st041558|FD-Score:-2.92|P-value:5.30E-4 ID:SGTC_1759|Compound:st043059|FD-Score:2.99|P-value:5.61E-4 ID:SGTC_1783|Compound:st039459|FD-Score:5.07|P-value:1.40E-8 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.64|P-value:3.54E-5 ID:SGTC_1863|Compound:st057641|FD-Score:-3.57|P-value:3.42E-5 ID:SGTC_1868|Compound:st057644|FD-Score:5.14|P-value:8.92E-9 ID:SGTC_1884|Compound:5633444|FD-Score:-3.31|P-value:1.08E-4 ID:SGTC_1920|Compound:st075728|FD-Score:3.04|P-value:4.55E-4 ID:SGTC_1946|Compound:st076057|FD-Score:3.11|P-value:3.48E-4 ID:SGTC_1961|Compound:st076550|FD-Score:3.23|P-value:2.12E-4 ID:SGTC_2044|Compound:5102272|FD-Score:-3.26|P-value:1.36E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:4.10|P-value:3.69E-6 ID:SGTC_2229|Compound:7354935|FD-Score:3.51|P-value:6.14E-5 ID:SGTC_2242|Compound:6630449|FD-Score:-2.79|P-value:8.97E-4 ID:SGTC_2288|Compound:7946289|FD-Score:3.37|P-value:1.16E-4 ID:SGTC_2309|Compound:7664088|FD-Score:3.21|P-value:2.27E-4 ID:SGTC_2324|Compound:6149169|FD-Score:3.00|P-value:5.42E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:-3.26|P-value:1.33E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:3.87|P-value:1.17E-5 ID:SGTC_2523|Compound:spermine|FD-Score:2.86|P-value:9.10E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:3.10|P-value:3.53E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:2.99|P-value:5.48E-4 ID:SGTC_2948|Compound:9070189|FD-Score:3.05|P-value:4.35E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-3.43|P-value:6.35E-5 ID:SGTC_2995|Compound:9067767|FD-Score:-3.48|P-value:5.17E-5 ID:SGTC_3197|Compound:9113331|FD-Score:-3.07|P-value:3.02E-4 ID:SGTC_3278|Compound:9139605|FD-Score:3.83|P-value:1.37E-5 ID:SGTC_3325|Compound:9138760|FD-Score:-3.19|P-value:1.79E-4 ID:SGTC_3327|Compound:9140648|FD-Score:5.30|P-value:3.11E-9 ID:SGTC_3335|Compound:9143315|FD-Score:-4.77|P-value:5.43E-8 ID:SGTC_51|Compound:1013-0126|FD-Score:4.72|P-value:1.14E-7 ID:SGTC_153|Compound:4077-0274|FD-Score:3.23|P-value:2.10E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:3.72|P-value:2.33E-5 ID:SGTC_277|Compound:nystatin|FD-Score:4.47|P-value:4.87E-7 ID:SGTC_530|Compound:4358-1714|FD-Score:3.30|P-value:1.56E-4 ID:SGTC_570|Compound:0987-0065|FD-Score:3.00|P-value:5.37E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:-3.05|P-value:3.18E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:2.84|P-value:9.66E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:3.24|P-value:2.03E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:3.01|P-value:5.16E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:5.27|P-value:3.78E-9 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-2.76|P-value:9.89E-4 ID:SGTC_1144|Compound:3486-0183|FD-Score:3.41|P-value:9.81E-5 ID:SGTC_1305|Compound:1181-0477|FD-Score:2.96|P-value:6.20E-4 ID:SGTC_1388|Compound:3346-4487|FD-Score:2.91|P-value:7.57E-4 ID:SGTC_1432|Compound:4049-0298|FD-Score:2.93|P-value:7.03E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:-3.08|P-value:2.82E-4 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.72|P-value:2.42E-5 ID:SGTC_1521|Compound:st012942|FD-Score:3.09|P-value:3.77E-4 ID:SGTC_1553|Compound:gossypol|FD-Score:3.88|P-value:1.10E-5 ID:SGTC_1608|Compound:st001473|FD-Score:2.87|P-value:8.93E-4 ID:SGTC_1618|Compound:st003135|FD-Score:3.17|P-value:2.74E-4 ID:SGTC_1634|Compound:st006576|FD-Score:3.72|P-value:2.43E-5 ID:SGTC_1742|Compound:st038235|FD-Score:3.07|P-value:3.99E-4 ID:SGTC_1756|Compound:st041558|FD-Score:-2.92|P-value:5.30E-4 ID:SGTC_1759|Compound:st043059|FD-Score:2.99|P-value:5.61E-4 ID:SGTC_1783|Compound:st039459|FD-Score:5.07|P-value:1.40E-8 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.64|P-value:3.54E-5 ID:SGTC_1863|Compound:st057641|FD-Score:-3.57|P-value:3.42E-5 ID:SGTC_1868|Compound:st057644|FD-Score:5.14|P-value:8.92E-9 ID:SGTC_1884|Compound:5633444|FD-Score:-3.31|P-value:1.08E-4 ID:SGTC_1920|Compound:st075728|FD-Score:3.04|P-value:4.55E-4 ID:SGTC_1946|Compound:st076057|FD-Score:3.11|P-value:3.48E-4 ID:SGTC_1961|Compound:st076550|FD-Score:3.23|P-value:2.12E-4 ID:SGTC_2044|Compound:5102272|FD-Score:-3.26|P-value:1.36E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:4.10|P-value:3.69E-6 ID:SGTC_2229|Compound:7354935|FD-Score:3.51|P-value:6.14E-5 ID:SGTC_2242|Compound:6630449|FD-Score:-2.79|P-value:8.97E-4 ID:SGTC_2288|Compound:7946289|FD-Score:3.37|P-value:1.16E-4 ID:SGTC_2309|Compound:7664088|FD-Score:3.21|P-value:2.27E-4 ID:SGTC_2324|Compound:6149169|FD-Score:3.00|P-value:5.42E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:-3.26|P-value:1.33E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:3.87|P-value:1.17E-5 ID:SGTC_2523|Compound:spermine|FD-Score:2.86|P-value:9.10E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:3.10|P-value:3.53E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:2.99|P-value:5.48E-4 ID:SGTC_2948|Compound:9070189|FD-Score:3.05|P-value:4.35E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-3.43|P-value:6.35E-5 ID:SGTC_2995|Compound:9067767|FD-Score:-3.48|P-value:5.17E-5 ID:SGTC_3197|Compound:9113331|FD-Score:-3.07|P-value:3.02E-4 ID:SGTC_3278|Compound:9139605|FD-Score:3.83|P-value:1.37E-5 ID:SGTC_3325|Compound:9138760|FD-Score:-3.19|P-value:1.79E-4 ID:SGTC_3327|Compound:9140648|FD-Score:5.30|P-value:3.11E-9 ID:SGTC_3335|Compound:9143315|FD-Score:-4.77|P-value:5.43E-8

Top fitness defect scores for YOL066C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3327 9140648 5.30 26.9 µM 3.11E-9 4 5.87E-8 0.06
2 SGTC_969 1313-0238 5.27 134.0 µM 3.78E-9 mitochondrial processes 8 6.89E-8 0.01
3 SGTC_1868 st057644 5.14 42.7 µM 8.92E-9 4 1.40E-7 0.91
4 SGTC_1783 st039459 5.07 87.5 µM 1.40E-8 Golgi 5 2.04E-7 0.17
5 SGTC_51 1013-0126 4.72 23.4 µM 1.14E-7 2 1.17E-6 0.35
6 SGTC_277 nystatin 4.47 15.5 µM 4.87E-7 2 3.90E-6 0.47
7 SGTC_2209 5-(5-methyl-2-thenylidene)-Barbituric acid 4.10 122.8 µM 3.69E-6 5 2.11E-5 0.03
8 SGTC_1553 gossypol 3.88 38.6 µM 1.10E-5 1 5.25E-5 0.58
9 SGTC_2515 chrysin 3.87 20.8 µM 1.17E-5 RPP1 & pyrimidine depletion 7 5.52E-5 0.43
10 SGTC_3278 9139605 3.83 49.5 µM 1.37E-5 10 6.29E-5 0.07
11 SGTC_163 1327-0036 3.72 7.5 µM 2.33E-5 49 9.80E-5 0.04
12 SGTC_1506 4226-2149 3.72 212.0 µM 2.42E-5 6 1.01E-4 0.13
13 SGTC_1634 st006576 3.72 41.0 µM 2.43E-5 TSC3-RPN4 10 1.01E-4 0.06
14 SGTC_1785 bathophenanthroline 3.64 24.0 µM 3.54E-5 heme requiring 27 1.39E-4 0.03
15 SGTC_2229 7354935 3.51 190.0 µM 6.14E-5 RSC complex & mRNA processing 23 2.20E-4 0.03
16 SGTC_1144 3486-0183 3.41 12.5 µM 9.81E-5 8 3.26E-4 0.13
17 SGTC_2288 7946289 3.37 167.3 µM 1.16E-4 9 3.75E-4 0.01
18 SGTC_530 4358-1714 3.30 28.0 µM 1.56E-4 12 4.80E-4 0.04
19 SGTC_958 1222-0007 3.24 33.0 µM 2.03E-4 17 6.00E-4 0.02
20 SGTC_153 4077-0274 3.23 519.7 µM 2.10E-4 14 6.17E-4 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.116 1.72E-11 YHR078W_p YHR078W_p hom High osmolarity-regulated gene of unknown function
0.103 1.88E-9 YHR098C SFB3 hom Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p
0.095 3.75E-8 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.093 5.86E-8 YGR193C PDX1 hom Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core
0.090 2.01E-7 YJR062C NTA1 hom Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation
0.085 7.83E-7 YBR160W CDC28 het Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress
0.085 8.45E-7 YER052C HOM3 hom Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.084 1.10E-6 YLR182W SWI6 hom Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators
0.084 1.13E-6 YMR007W_d YMR007W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.084 1.25E-6 YOR375C GDH1 hom NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication
0.083 1.71E-6 YKL108W SLD2 het Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress
0.078 5.46E-6 YGR224W AZR1 hom Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole
0.078 6.33E-6 YOR029W_d YOR029W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.077 7.38E-6 YIR008C PRI1 het Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.075 1.57E-5 YNL181W_p YNL181W_p het Putative oxidoreductase; required for cell viability