YOL069W / NUF2

Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOL069W / NUF2

Click on Significant Values for Screen Details ID:SGTC_104|Compound:0074-0074|FD-Score:-3.47|P-value:2.18E-4 ID:SGTC_107|Compound:0358-0022|FD-Score:3.56|P-value:7.52E-5 ID:SGTC_117|Compound:lucigenin|FD-Score:6.17|P-value:4.01E-11 ID:SGTC_130|Compound:0136-0219|FD-Score:3.22|P-value:2.95E-4 ID:SGTC_195|Compound:4234-0266|FD-Score:-3.13|P-value:7.60E-4 ID:SGTC_213|Compound:1246-0773|FD-Score:-3.08|P-value:9.20E-4 ID:SGTC_252|Compound:miconazole|FD-Score:-3.90|P-value:3.63E-5 ID:SGTC_379|Compound:3970-0972|FD-Score:4.38|P-value:1.69E-6 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-3.26|P-value:4.74E-4 ID:SGTC_397|Compound:arbidol|FD-Score:3.13|P-value:4.20E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:6.26|P-value:2.05E-11 ID:SGTC_604|Compound:1000-0114|FD-Score:4.39|P-value:1.65E-6 ID:SGTC_612|Compound:0302-0167|FD-Score:-3.26|P-value:4.76E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.31|P-value:2.11E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:3.73|P-value:3.67E-5 ID:SGTC_884|Compound:0851-0618|FD-Score:4.97|P-value:7.19E-8 ID:SGTC_910|Compound:3454-3003|FD-Score:3.18|P-value:3.41E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:5.34|P-value:8.48E-9 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.22|P-value:5.50E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.36|P-value:1.67E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-6.40|P-value:2.49E-11 ID:SGTC_1097|Compound:itavastatin|FD-Score:5.08|P-value:3.89E-8 ID:SGTC_1183|Compound:1254-0324|FD-Score:2.97|P-value:7.35E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:5.26|P-value:1.35E-8 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.32|P-value:2.02E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:3.27|P-value:2.38E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:4.17|P-value:4.75E-6 ID:SGTC_1678|Compound:st019265|FD-Score:-3.28|P-value:4.40E-4 ID:SGTC_1727|Compound:st034309|FD-Score:4.65|P-value:4.20E-7 ID:SGTC_1745|Compound:st081495|FD-Score:-5.21|P-value:5.07E-8 ID:SGTC_1784|Compound:st039334|FD-Score:4.55|P-value:7.29E-7 ID:SGTC_1788|Compound:fluconazole|FD-Score:-3.30|P-value:4.14E-4 ID:SGTC_1807|Compound:st050092|FD-Score:4.50|P-value:9.42E-7 ID:SGTC_1903|Compound:st060211|FD-Score:3.92|P-value:1.56E-5 ID:SGTC_1920|Compound:st075728|FD-Score:-3.37|P-value:3.14E-4 ID:SGTC_1921|Compound:5740136|FD-Score:-3.35|P-value:3.43E-4 ID:SGTC_1922|Compound:5578187|FD-Score:-3.24|P-value:5.16E-4 ID:SGTC_1924|Compound:st058459|FD-Score:3.06|P-value:5.44E-4 ID:SGTC_1929|Compound:genistein trimethyl ether|FD-Score:-6.01|P-value:3.56E-10 ID:SGTC_1931|Compound:st074438|FD-Score:3.60|P-value:6.30E-5 ID:SGTC_1991|Compound:st073000|FD-Score:-4.04|P-value:1.93E-5 ID:SGTC_2050|Compound:5263056|FD-Score:3.34|P-value:1.82E-4 ID:SGTC_2058|Compound:5236698|FD-Score:-3.67|P-value:9.82E-5 ID:SGTC_2078|Compound:5212016|FD-Score:4.51|P-value:8.93E-7 ID:SGTC_2302|Compound:7771753|FD-Score:5.75|P-value:6.25E-10 ID:SGTC_2303|Compound:7774051|FD-Score:-5.50|P-value:9.21E-9 ID:SGTC_2313|Compound:7726885|FD-Score:3.19|P-value:3.33E-4 ID:SGTC_2329|Compound:6138490|FD-Score:2.95|P-value:7.95E-4 ID:SGTC_2360|Compound:9040895|FD-Score:3.03|P-value:5.89E-4 ID:SGTC_2367|Compound:9070478|FD-Score:4.58|P-value:6.22E-7 ID:SGTC_2412|Compound:st077135|FD-Score:3.57|P-value:7.05E-5 ID:SGTC_2515|Compound:chrysin|FD-Score:-3.19|P-value:6.20E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:-3.46|P-value:2.26E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.17|P-value:3.50E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:3.84|P-value:2.21E-5 ID:SGTC_2863|Compound:9028737|FD-Score:3.82|P-value:2.43E-5 ID:SGTC_2918|Compound:7977171|FD-Score:3.72|P-value:3.71E-5 ID:SGTC_2923|Compound:7992654|FD-Score:-3.98|P-value:2.55E-5 ID:SGTC_2925|Compound:9010536|FD-Score:-5.17|P-value:6.23E-8 ID:SGTC_2955|Compound:9076254|FD-Score:2.97|P-value:7.32E-4 ID:SGTC_2957|Compound:9076800|FD-Score:3.54|P-value:8.13E-5 ID:SGTC_2965|Compound:9082772|FD-Score:-4.52|P-value:2.00E-6 ID:SGTC_3014|Compound:9081533|FD-Score:4.03|P-value:9.13E-6 ID:SGTC_3324|Compound:9138759|FD-Score:-7.18|P-value:7.98E-14 ID:SGTC_3326|Compound:9140064|FD-Score:4.28|P-value:2.81E-6 ID:SGTC_3330|Compound:9141424|FD-Score:-3.09|P-value:8.88E-4 ID:SGTC_104|Compound:0074-0074|FD-Score:-3.47|P-value:2.18E-4 ID:SGTC_107|Compound:0358-0022|FD-Score:3.56|P-value:7.52E-5 ID:SGTC_117|Compound:lucigenin|FD-Score:6.17|P-value:4.01E-11 ID:SGTC_130|Compound:0136-0219|FD-Score:3.22|P-value:2.95E-4 ID:SGTC_195|Compound:4234-0266|FD-Score:-3.13|P-value:7.60E-4 ID:SGTC_213|Compound:1246-0773|FD-Score:-3.08|P-value:9.20E-4 ID:SGTC_252|Compound:miconazole|FD-Score:-3.90|P-value:3.63E-5 ID:SGTC_379|Compound:3970-0972|FD-Score:4.38|P-value:1.69E-6 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-3.26|P-value:4.74E-4 ID:SGTC_397|Compound:arbidol|FD-Score:3.13|P-value:4.20E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:6.26|P-value:2.05E-11 ID:SGTC_604|Compound:1000-0114|FD-Score:4.39|P-value:1.65E-6 ID:SGTC_612|Compound:0302-0167|FD-Score:-3.26|P-value:4.76E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.31|P-value:2.11E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:3.73|P-value:3.67E-5 ID:SGTC_884|Compound:0851-0618|FD-Score:4.97|P-value:7.19E-8 ID:SGTC_910|Compound:3454-3003|FD-Score:3.18|P-value:3.41E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:5.34|P-value:8.48E-9 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.22|P-value:5.50E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.36|P-value:1.67E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-6.40|P-value:2.49E-11 ID:SGTC_1097|Compound:itavastatin|FD-Score:5.08|P-value:3.89E-8 ID:SGTC_1183|Compound:1254-0324|FD-Score:2.97|P-value:7.35E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:5.26|P-value:1.35E-8 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.32|P-value:2.02E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:3.27|P-value:2.38E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:4.17|P-value:4.75E-6 ID:SGTC_1678|Compound:st019265|FD-Score:-3.28|P-value:4.40E-4 ID:SGTC_1727|Compound:st034309|FD-Score:4.65|P-value:4.20E-7 ID:SGTC_1745|Compound:st081495|FD-Score:-5.21|P-value:5.07E-8 ID:SGTC_1784|Compound:st039334|FD-Score:4.55|P-value:7.29E-7 ID:SGTC_1788|Compound:fluconazole|FD-Score:-3.30|P-value:4.14E-4 ID:SGTC_1807|Compound:st050092|FD-Score:4.50|P-value:9.42E-7 ID:SGTC_1903|Compound:st060211|FD-Score:3.92|P-value:1.56E-5 ID:SGTC_1920|Compound:st075728|FD-Score:-3.37|P-value:3.14E-4 ID:SGTC_1921|Compound:5740136|FD-Score:-3.35|P-value:3.43E-4 ID:SGTC_1922|Compound:5578187|FD-Score:-3.24|P-value:5.16E-4 ID:SGTC_1924|Compound:st058459|FD-Score:3.06|P-value:5.44E-4 ID:SGTC_1929|Compound:genistein trimethyl ether|FD-Score:-6.01|P-value:3.56E-10 ID:SGTC_1931|Compound:st074438|FD-Score:3.60|P-value:6.30E-5 ID:SGTC_1991|Compound:st073000|FD-Score:-4.04|P-value:1.93E-5 ID:SGTC_2050|Compound:5263056|FD-Score:3.34|P-value:1.82E-4 ID:SGTC_2058|Compound:5236698|FD-Score:-3.67|P-value:9.82E-5 ID:SGTC_2078|Compound:5212016|FD-Score:4.51|P-value:8.93E-7 ID:SGTC_2302|Compound:7771753|FD-Score:5.75|P-value:6.25E-10 ID:SGTC_2303|Compound:7774051|FD-Score:-5.50|P-value:9.21E-9 ID:SGTC_2313|Compound:7726885|FD-Score:3.19|P-value:3.33E-4 ID:SGTC_2329|Compound:6138490|FD-Score:2.95|P-value:7.95E-4 ID:SGTC_2360|Compound:9040895|FD-Score:3.03|P-value:5.89E-4 ID:SGTC_2367|Compound:9070478|FD-Score:4.58|P-value:6.22E-7 ID:SGTC_2412|Compound:st077135|FD-Score:3.57|P-value:7.05E-5 ID:SGTC_2515|Compound:chrysin|FD-Score:-3.19|P-value:6.20E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:-3.46|P-value:2.26E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.17|P-value:3.50E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:3.84|P-value:2.21E-5 ID:SGTC_2863|Compound:9028737|FD-Score:3.82|P-value:2.43E-5 ID:SGTC_2918|Compound:7977171|FD-Score:3.72|P-value:3.71E-5 ID:SGTC_2923|Compound:7992654|FD-Score:-3.98|P-value:2.55E-5 ID:SGTC_2925|Compound:9010536|FD-Score:-5.17|P-value:6.23E-8 ID:SGTC_2955|Compound:9076254|FD-Score:2.97|P-value:7.32E-4 ID:SGTC_2957|Compound:9076800|FD-Score:3.54|P-value:8.13E-5 ID:SGTC_2965|Compound:9082772|FD-Score:-4.52|P-value:2.00E-6 ID:SGTC_3014|Compound:9081533|FD-Score:4.03|P-value:9.13E-6 ID:SGTC_3324|Compound:9138759|FD-Score:-7.18|P-value:7.98E-14 ID:SGTC_3326|Compound:9140064|FD-Score:4.28|P-value:2.81E-6 ID:SGTC_3330|Compound:9141424|FD-Score:-3.09|P-value:8.88E-4

Top fitness defect scores for YOL069W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_514 4466-0038 6.26 2.6 nM 2.05E-11 cell wall 13 1.91E-10 0.12
2 SGTC_117 lucigenin 6.17 22.1 µM 4.01E-11 redox potentiating 4 3.52E-10 0.26
3 SGTC_2302 7771753 5.75 200.0 µM 6.25E-10 4 4.33E-9 1.30
4 SGTC_916 4466-0038 5.34 42.0 nM 8.48E-9 cell wall 17 4.69E-8 0.45
5 SGTC_1247 0448-0043 5.26 5.8 µM 1.35E-8 ERG2 4 7.16E-8 0.07
6 SGTC_1097 itavastatin 5.08 7.2 µM 3.89E-8 azole & statin 5 1.88E-7 0.22
7 SGTC_884 0851-0618 4.97 334.0 µM 7.19E-8 1 3.30E-7 0.66
8 SGTC_1727 st034309 4.65 9.9 µM 4.20E-7 10 1.65E-6 0.26
9 SGTC_2367 9070478 4.58 135.4 µM 6.22E-7 cell wall 6 2.36E-6 0.20
10 SGTC_1784 st039334 4.55 36.3 µM 7.29E-7 5 2.73E-6 0.19
11 SGTC_2078 5212016 4.51 200.0 µM 8.93E-7 60S ribosome export 5 3.28E-6 0.50
12 SGTC_1807 st050092 4.50 58.9 µM 9.42E-7 60S ribosome export 2 3.45E-6 0.06
13 SGTC_604 1000-0114 4.39 314.0 µM 1.65E-6 2 5.75E-6 0.10
14 SGTC_379 3970-0972 4.38 5.9 µM 1.69E-6 tubulin folding & SWR complex 5 5.88E-6 0.41
15 SGTC_3326 9140064 4.28 16.4 µM 2.81E-6 4 9.33E-6 0.36
16 SGTC_1379 2144-0755 4.17 36.5 µM 4.75E-6 4 1.50E-5 0.25
17 SGTC_3014 9081533 4.03 49.5 µM 9.13E-6 2 2.73E-5 0.24
18 SGTC_1903 st060211 3.92 78.3 µM 1.56E-5 2 4.44E-5 0.06
19 SGTC_2593 nobiletin 3.84 86.2 µM 2.21E-5 12 6.10E-5 0.03
20 SGTC_2863 9028737 3.82 58.4 µM 2.43E-5 1 6.66E-5 0.50

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.175 1.56E-24 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.171 1.76E-23 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.150 3.01E-18 YDR121W DPB4 hom Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
0.140 3.35E-16 YKL053W_d YKL053W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1
0.140 4.24E-16 YMR036C MIH1 hom Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.139 5.73E-16 YML117W NAB6 hom Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
0.135 5.64E-15 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.127 1.86E-13 YDR483W KRE2 hom Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication
0.123 7.56E-13 YOR091W TMA46 hom Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
0.122 1.14E-12 YDR053W_d YDR053W_d het Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex
0.121 2.16E-12 YGR211W ZPR1 het Essential protein with two zinc fingers; present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p); relative distribution to the nucleus increases upon DNA replication stress
0.119 5.27E-12 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.119 5.56E-12 YHL044W_p YHL044W_p hom Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern
0.117 9.57E-12 YGR284C ERV29 hom Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress
0.117 1.21E-11 YDL134C PPH21 hom Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication