HIPHOP chemogenomics database

YOL077C / BRX1

Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif

Zygosity: Heterozygous strain

fixedexpanded
Profile for YOL077C / BRX1

Click on Significant Values for Screen Details

Top fitness defect scores for YOL077C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_2782	7736369	14.60	71.4 µM	3.22E-52		1	6.95E-49	9.04
2	SGTC_195	4234-0266	11.80	16.6 µM	1.13E-34		1	1.82E-32	4.96
3	SGTC_2438	5259590	8.21	200.0 µM	8.24E-18		1	1.08E-16	2.86
4	SGTC_1074	carmofur	7.29	3.8 µM	1.93E-14	exosome	9	1.53E-13	0.12
5	SGTC_2445	5742597	5.90	35.6 µM	4.59E-10		1	1.87E-9	1.53
6	SGTC_2327	6189656	5.85	200.0 µM	6.04E-10		3	2.42E-9	1.05
7	SGTC_2787	5284540	5.19	71.4 µM	3.38E-8		2	1.04E-7	0.03
8	SGTC_2628	lathosterol	5.13	100.0 µM	4.74E-8		4	1.42E-7	0.24
9	SGTC_2453	5731132	4.93	22.2 µM	1.51E-7		2	4.21E-7	0.50
10	SGTC_2785	7756463	4.87	72.7 µM	2.05E-7		7	5.58E-7	0.02
11	SGTC_369	0957-0393	4.52	4.5 µM	1.28E-6	cell wall signaling	3	3.10E-6	0.13
12	SGTC_1714	st033236	4.44	13.6 µM	1.90E-6		5	4.47E-6	0.04
13	SGTC_1077	5-fluorocytosine	4.39	377.0 nM	2.45E-6	exosome	15	5.66E-6	0.18
14	SGTC_2916	7875993	4.38	68.8 µM	2.53E-6		3	5.84E-6	0.11
15	SGTC_1940	st075990	4.30	51.1 µM	3.77E-6	60S ribosome export	5	8.47E-6	0.21
16	SGTC_1131	3454-3128	4.27	32.3 µM	4.30E-6		12	9.58E-6	0.25
17	SGTC_820	1582-0060	4.26	59.9 µM	4.71E-6		9	1.04E-5	0.06
18	SGTC_2326	6164455	4.24	177.6 µM	5.02E-6	mitochondrial processes	10	1.11E-5	0.03
19	SGTC_689	0833-0039	4.23	98.0 µM	5.20E-6	ergosterol depletion effects on membrane	8	1.14E-5	0.16
20	SGTC_2681	menadione	4.21	3.2 µM	5.95E-6	superoxide	2	1.30E-5	0.07

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.227	2.10E-40	YGR195W	SKI6	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.182	1.87E-26	YDL111C	RRP42	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
0.180	6.99E-26	YOL021C	DIS3	het	Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
0.164	1.38E-21	YPR142C_d	YPR142C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
0.163	2.20E-21	YPL044C_d	YPL044C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.158	3.17E-20	YPL043W	NOP4	het	Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
0.154	2.81E-19	YGR158C	MTR3	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
0.138	8.10E-16	YGR095C	RRP46	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
0.137	1.93E-15	YDR280W	RRP45	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
0.132	1.57E-14	YKL172W	EBP2	het	Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.132	2.03E-14	YOR095C	RKI1	het	Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
0.124	5.83E-13	YNL002C	RLP7	het	Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
0.122	1.15E-12	YMR148W	OSW5	hom	Protein of unknown function that may play a role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion
0.122	1.31E-12	YPR143W	RRP15	het	Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
0.115	2.33E-11	YKL021C	MAK11	het	Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats