YOL077C / BRX1

Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOL077C / BRX1

Click on Significant Values for Screen Details ID:SGTC_40|Compound:0327-0103|FD-Score:3.44|P-value:1.67E-4 ID:SGTC_147|Compound:4466-0538|FD-Score:3.03|P-value:7.90E-4 ID:SGTC_195|Compound:4234-0266|FD-Score:11.80|P-value:1.13E-34 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.23|P-value:3.70E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:3.82|P-value:3.49E-5 ID:SGTC_369|Compound:0957-0393|FD-Score:4.52|P-value:1.28E-6 ID:SGTC_394|Compound:menadione|FD-Score:3.73|P-value:5.01E-5 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.11|P-value:5.82E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:4.06|P-value:1.20E-5 ID:SGTC_641|Compound:4092-0595|FD-Score:3.70|P-value:5.87E-5 ID:SGTC_689|Compound:0833-0039|FD-Score:4.23|P-value:5.20E-6 ID:SGTC_820|Compound:1582-0060|FD-Score:4.26|P-value:4.71E-6 ID:SGTC_834|Compound:0312-0008|FD-Score:-4.15|P-value:1.05E-5 ID:SGTC_839|Compound:0122-0028|FD-Score:-4.34|P-value:4.30E-6 ID:SGTC_852|Compound:0418-0143|FD-Score:-3.33|P-value:3.31E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:-3.19|P-value:5.54E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:3.03|P-value:7.88E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:7.29|P-value:1.93E-14 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:4.39|P-value:2.45E-6 ID:SGTC_1122|Compound:capsaicin|FD-Score:2.99|P-value:8.95E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:4.27|P-value:4.30E-6 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.24|P-value:3.67E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.16|P-value:4.95E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:-3.03|P-value:9.87E-4 ID:SGTC_1571|Compound:st070080|FD-Score:4.19|P-value:6.57E-6 ID:SGTC_1573|Compound:oleylamine|FD-Score:-3.62|P-value:1.05E-4 ID:SGTC_1590|Compound:st077108|FD-Score:3.46|P-value:1.56E-4 ID:SGTC_1642|Compound:st009582|FD-Score:3.53|P-value:1.14E-4 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:3.28|P-value:3.06E-4 ID:SGTC_1714|Compound:st033236|FD-Score:4.44|P-value:1.90E-6 ID:SGTC_1907|Compound:st060210|FD-Score:3.01|P-value:8.27E-4 ID:SGTC_1940|Compound:st075990|FD-Score:4.30|P-value:3.77E-6 ID:SGTC_2022|Compound:5136654|FD-Score:3.32|P-value:2.70E-4 ID:SGTC_2031|Compound:5180455|FD-Score:3.85|P-value:3.04E-5 ID:SGTC_2040|Compound:5100729|FD-Score:4.05|P-value:1.22E-5 ID:SGTC_2094|Compound:5332512|FD-Score:3.25|P-value:3.48E-4 ID:SGTC_2108|Compound:5365811|FD-Score:3.06|P-value:6.87E-4 ID:SGTC_2145|Compound:5634607|FD-Score:-3.09|P-value:8.13E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:3.59|P-value:9.08E-5 ID:SGTC_2319|Compound:6033012|FD-Score:-7.23|P-value:5.43E-14 ID:SGTC_2323|Compound:6148992|FD-Score:-4.51|P-value:1.87E-6 ID:SGTC_2326|Compound:6164455|FD-Score:4.24|P-value:5.02E-6 ID:SGTC_2327|Compound:6189656|FD-Score:5.85|P-value:6.04E-10 ID:SGTC_2358|Compound:9037157|FD-Score:3.94|P-value:2.05E-5 ID:SGTC_2438|Compound:5259590|FD-Score:8.21|P-value:8.24E-18 ID:SGTC_2439|Compound:5492030|FD-Score:3.51|P-value:1.28E-4 ID:SGTC_2445|Compound:5742597|FD-Score:5.90|P-value:4.59E-10 ID:SGTC_2453|Compound:5731132|FD-Score:4.93|P-value:1.51E-7 ID:SGTC_2495|Compound:cafestol|FD-Score:-3.05|P-value:9.18E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.27|P-value:3.17E-4 ID:SGTC_2526|Compound:patulin|FD-Score:3.62|P-value:7.94E-5 ID:SGTC_2628|Compound:lathosterol|FD-Score:5.13|P-value:4.74E-8 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.56|P-value:1.05E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.27|P-value:3.27E-4 ID:SGTC_2681|Compound:menadione|FD-Score:4.21|P-value:5.95E-6 ID:SGTC_2780|Compound:5215298|FD-Score:3.02|P-value:8.10E-4 ID:SGTC_2782|Compound:7736369|FD-Score:14.60|P-value:3.22E-52 ID:SGTC_2785|Compound:7756463|FD-Score:4.87|P-value:2.05E-7 ID:SGTC_2787|Compound:5284540|FD-Score:5.19|P-value:3.38E-8 ID:SGTC_2820|Compound:7989630|FD-Score:-3.21|P-value:5.22E-4 ID:SGTC_2821|Compound:7996764|FD-Score:-3.09|P-value:7.99E-4 ID:SGTC_2900|Compound:5328528|FD-Score:-3.74|P-value:6.39E-5 ID:SGTC_2902|Compound:5787006|FD-Score:-4.20|P-value:8.34E-6 ID:SGTC_2914|Compound:7968218|FD-Score:-3.92|P-value:2.97E-5 ID:SGTC_2916|Compound:7875993|FD-Score:4.38|P-value:2.53E-6 ID:SGTC_2978|Compound:9011784|FD-Score:-3.42|P-value:2.39E-4 ID:SGTC_3047|Compound:9092122|FD-Score:-3.12|P-value:7.27E-4 ID:SGTC_3217|Compound:9129673|FD-Score:3.85|P-value:3.01E-5 ID:SGTC_40|Compound:0327-0103|FD-Score:3.44|P-value:1.67E-4 ID:SGTC_147|Compound:4466-0538|FD-Score:3.03|P-value:7.90E-4 ID:SGTC_195|Compound:4234-0266|FD-Score:11.80|P-value:1.13E-34 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.23|P-value:3.70E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:3.82|P-value:3.49E-5 ID:SGTC_369|Compound:0957-0393|FD-Score:4.52|P-value:1.28E-6 ID:SGTC_394|Compound:menadione|FD-Score:3.73|P-value:5.01E-5 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.11|P-value:5.82E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:4.06|P-value:1.20E-5 ID:SGTC_641|Compound:4092-0595|FD-Score:3.70|P-value:5.87E-5 ID:SGTC_689|Compound:0833-0039|FD-Score:4.23|P-value:5.20E-6 ID:SGTC_820|Compound:1582-0060|FD-Score:4.26|P-value:4.71E-6 ID:SGTC_834|Compound:0312-0008|FD-Score:-4.15|P-value:1.05E-5 ID:SGTC_839|Compound:0122-0028|FD-Score:-4.34|P-value:4.30E-6 ID:SGTC_852|Compound:0418-0143|FD-Score:-3.33|P-value:3.31E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:-3.19|P-value:5.54E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:3.03|P-value:7.88E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:7.29|P-value:1.93E-14 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:4.39|P-value:2.45E-6 ID:SGTC_1122|Compound:capsaicin|FD-Score:2.99|P-value:8.95E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:4.27|P-value:4.30E-6 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.24|P-value:3.67E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.16|P-value:4.95E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:-3.03|P-value:9.87E-4 ID:SGTC_1571|Compound:st070080|FD-Score:4.19|P-value:6.57E-6 ID:SGTC_1573|Compound:oleylamine|FD-Score:-3.62|P-value:1.05E-4 ID:SGTC_1590|Compound:st077108|FD-Score:3.46|P-value:1.56E-4 ID:SGTC_1642|Compound:st009582|FD-Score:3.53|P-value:1.14E-4 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:3.28|P-value:3.06E-4 ID:SGTC_1714|Compound:st033236|FD-Score:4.44|P-value:1.90E-6 ID:SGTC_1907|Compound:st060210|FD-Score:3.01|P-value:8.27E-4 ID:SGTC_1940|Compound:st075990|FD-Score:4.30|P-value:3.77E-6 ID:SGTC_2022|Compound:5136654|FD-Score:3.32|P-value:2.70E-4 ID:SGTC_2031|Compound:5180455|FD-Score:3.85|P-value:3.04E-5 ID:SGTC_2040|Compound:5100729|FD-Score:4.05|P-value:1.22E-5 ID:SGTC_2094|Compound:5332512|FD-Score:3.25|P-value:3.48E-4 ID:SGTC_2108|Compound:5365811|FD-Score:3.06|P-value:6.87E-4 ID:SGTC_2145|Compound:5634607|FD-Score:-3.09|P-value:8.13E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:3.59|P-value:9.08E-5 ID:SGTC_2319|Compound:6033012|FD-Score:-7.23|P-value:5.43E-14 ID:SGTC_2323|Compound:6148992|FD-Score:-4.51|P-value:1.87E-6 ID:SGTC_2326|Compound:6164455|FD-Score:4.24|P-value:5.02E-6 ID:SGTC_2327|Compound:6189656|FD-Score:5.85|P-value:6.04E-10 ID:SGTC_2358|Compound:9037157|FD-Score:3.94|P-value:2.05E-5 ID:SGTC_2438|Compound:5259590|FD-Score:8.21|P-value:8.24E-18 ID:SGTC_2439|Compound:5492030|FD-Score:3.51|P-value:1.28E-4 ID:SGTC_2445|Compound:5742597|FD-Score:5.90|P-value:4.59E-10 ID:SGTC_2453|Compound:5731132|FD-Score:4.93|P-value:1.51E-7 ID:SGTC_2495|Compound:cafestol|FD-Score:-3.05|P-value:9.18E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.27|P-value:3.17E-4 ID:SGTC_2526|Compound:patulin|FD-Score:3.62|P-value:7.94E-5 ID:SGTC_2628|Compound:lathosterol|FD-Score:5.13|P-value:4.74E-8 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.56|P-value:1.05E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.27|P-value:3.27E-4 ID:SGTC_2681|Compound:menadione|FD-Score:4.21|P-value:5.95E-6 ID:SGTC_2780|Compound:5215298|FD-Score:3.02|P-value:8.10E-4 ID:SGTC_2782|Compound:7736369|FD-Score:14.60|P-value:3.22E-52 ID:SGTC_2785|Compound:7756463|FD-Score:4.87|P-value:2.05E-7 ID:SGTC_2787|Compound:5284540|FD-Score:5.19|P-value:3.38E-8 ID:SGTC_2820|Compound:7989630|FD-Score:-3.21|P-value:5.22E-4 ID:SGTC_2821|Compound:7996764|FD-Score:-3.09|P-value:7.99E-4 ID:SGTC_2900|Compound:5328528|FD-Score:-3.74|P-value:6.39E-5 ID:SGTC_2902|Compound:5787006|FD-Score:-4.20|P-value:8.34E-6 ID:SGTC_2914|Compound:7968218|FD-Score:-3.92|P-value:2.97E-5 ID:SGTC_2916|Compound:7875993|FD-Score:4.38|P-value:2.53E-6 ID:SGTC_2978|Compound:9011784|FD-Score:-3.42|P-value:2.39E-4 ID:SGTC_3047|Compound:9092122|FD-Score:-3.12|P-value:7.27E-4 ID:SGTC_3217|Compound:9129673|FD-Score:3.85|P-value:3.01E-5

Top fitness defect scores for YOL077C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2782 7736369 14.60 71.4 µM 3.22E-52 1 6.95E-49 9.04
2 SGTC_195 4234-0266 11.80 16.6 µM 1.13E-34 1 1.82E-32 4.96
3 SGTC_2438 5259590 8.21 200.0 µM 8.24E-18 1 1.08E-16 2.86
4 SGTC_1074 carmofur 7.29 3.8 µM 1.93E-14 exosome 9 1.53E-13 0.12
5 SGTC_2445 5742597 5.90 35.6 µM 4.59E-10 1 1.87E-9 1.53
6 SGTC_2327 6189656 5.85 200.0 µM 6.04E-10 3 2.42E-9 1.05
7 SGTC_2787 5284540 5.19 71.4 µM 3.38E-8 2 1.04E-7 0.03
8 SGTC_2628 lathosterol 5.13 100.0 µM 4.74E-8 4 1.42E-7 0.24
9 SGTC_2453 5731132 4.93 22.2 µM 1.51E-7 2 4.21E-7 0.50
10 SGTC_2785 7756463 4.87 72.7 µM 2.05E-7 7 5.58E-7 0.02
11 SGTC_369 0957-0393 4.52 4.5 µM 1.28E-6 cell wall signaling 3 3.10E-6 0.13
12 SGTC_1714 st033236 4.44 13.6 µM 1.90E-6 5 4.47E-6 0.04
13 SGTC_1077 5-fluorocytosine 4.39 377.0 nM 2.45E-6 exosome 15 5.66E-6 0.18
14 SGTC_2916 7875993 4.38 68.8 µM 2.53E-6 3 5.84E-6 0.11
15 SGTC_1940 st075990 4.30 51.1 µM 3.77E-6 60S ribosome export 5 8.47E-6 0.21
16 SGTC_1131 3454-3128 4.27 32.3 µM 4.30E-6 12 9.58E-6 0.25
17 SGTC_820 1582-0060 4.26 59.9 µM 4.71E-6 9 1.04E-5 0.06
18 SGTC_2326 6164455 4.24 177.6 µM 5.02E-6 mitochondrial processes 10 1.11E-5 0.03
19 SGTC_689 0833-0039 4.23 98.0 µM 5.20E-6 ergosterol depletion effects on membrane 8 1.14E-5 0.16
20 SGTC_2681 menadione 4.21 3.2 µM 5.95E-6 superoxide 2 1.30E-5 0.07

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.227 2.10E-40 YGR195W SKI6 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.182 1.87E-26 YDL111C RRP42 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
0.180 6.99E-26 YOL021C DIS3 het Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
0.164 1.38E-21 YPR142C_d YPR142C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
0.163 2.20E-21 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.158 3.17E-20 YPL043W NOP4 het Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
0.154 2.81E-19 YGR158C MTR3 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
0.138 8.10E-16 YGR095C RRP46 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
0.137 1.93E-15 YDR280W RRP45 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
0.132 1.57E-14 YKL172W EBP2 het Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.132 2.03E-14 YOR095C RKI1 het Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
0.124 5.83E-13 YNL002C RLP7 het Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
0.122 1.15E-12 YMR148W OSW5 hom Protein of unknown function that may play a role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion
0.122 1.31E-12 YPR143W RRP15 het Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
0.115 2.33E-11 YKL021C MAK11 het Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats