YOL081W / IRA2

GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL081W / IRA2

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:3.83|P-value:2.55E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.52|P-value:9.88E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:3.39|P-value:9.99E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:6.22|P-value:9.67E-9 ID:SGTC_168|Compound:3970-0862|FD-Score:8.23|P-value:6.38E-14 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.50|P-value:7.14E-4 ID:SGTC_262|Compound:fenpropimorph|FD-Score:5.64|P-value:1.71E-7 ID:SGTC_312|Compound:4092-0653|FD-Score:5.46|P-value:3.98E-7 ID:SGTC_349|Compound:k015-0014|FD-Score:3.92|P-value:1.88E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.53|P-value:6.54E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:3.47|P-value:7.89E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.75|P-value:1.38E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:-4.94|P-value:6.50E-6 ID:SGTC_515|Compound:4092-1064|FD-Score:4.45|P-value:2.78E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:12.10|P-value:8.28E-28 ID:SGTC_562|Compound:3772-2733|FD-Score:6.54|P-value:1.76E-9 ID:SGTC_658|Compound:3253-0951|FD-Score:4.85|P-value:5.68E-6 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.73|P-value:5.29E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:5.56|P-value:2.46E-7 ID:SGTC_690|Compound:glyoxal|FD-Score:7.33|P-value:1.93E-11 ID:SGTC_717|Compound:1532-0246|FD-Score:4.19|P-value:7.18E-5 ID:SGTC_727|Compound:3032-0951|FD-Score:4.69|P-value:1.06E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:5.19|P-value:1.34E-6 ID:SGTC_790|Compound:1575-0012|FD-Score:5.26|P-value:9.98E-7 ID:SGTC_919|Compound:tnp00227|FD-Score:3.42|P-value:9.17E-4 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:3.49|P-value:7.46E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:3.57|P-value:5.92E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:-4.61|P-value:2.40E-5 ID:SGTC_984|Compound:1492-0255|FD-Score:-4.34|P-value:6.69E-5 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:4.74|P-value:8.91E-6 ID:SGTC_1108|Compound:betulinic acid|FD-Score:3.46|P-value:8.26E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:4.28|P-value:5.24E-5 ID:SGTC_1166|Compound:k206-0078|FD-Score:-4.05|P-value:1.81E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:4.80|P-value:6.95E-6 ID:SGTC_1180|Compound:1193-0155|FD-Score:4.33|P-value:4.24E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:5.20|P-value:1.28E-6 ID:SGTC_1422|Compound:4031-0024|FD-Score:5.27|P-value:9.36E-7 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:9.22|P-value:5.12E-17 ID:SGTC_1712|Compound:st032288|FD-Score:5.85|P-value:6.43E-8 ID:SGTC_1715|Compound:st032160|FD-Score:3.58|P-value:5.74E-4 ID:SGTC_1800|Compound:st050079|FD-Score:4.48|P-value:2.47E-5 ID:SGTC_1803|Compound:st050068|FD-Score:3.87|P-value:2.20E-4 ID:SGTC_1847|Compound:st056231|FD-Score:3.54|P-value:6.47E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:3.61|P-value:5.10E-4 ID:SGTC_1875|Compound:nocodazole|FD-Score:6.59|P-value:1.35E-9 ID:SGTC_2038|Compound:4100038|FD-Score:3.99|P-value:1.45E-4 ID:SGTC_2215|Compound:7188065|FD-Score:4.86|P-value:5.43E-6 ID:SGTC_2233|Compound:sulbentine|FD-Score:4.02|P-value:1.29E-4 ID:SGTC_2237|Compound:6667747|FD-Score:4.37|P-value:3.70E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:6.92|P-value:2.15E-10 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.10|P-value:1.99E-6 ID:SGTC_2395|Compound:5646831|FD-Score:5.89|P-value:5.13E-8 ID:SGTC_2468|Compound:5987765|FD-Score:3.65|P-value:4.47E-4 ID:SGTC_2476|Compound:5806916|FD-Score:3.66|P-value:4.37E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:5.18|P-value:1.36E-6 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.54|P-value:6.43E-4 ID:SGTC_2857|Compound:9024691|FD-Score:4.19|P-value:7.08E-5 ID:SGTC_2866|Compound:9034245|FD-Score:3.48|P-value:7.62E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.59|P-value:5.52E-4 ID:SGTC_3071|Compound:9117417|FD-Score:3.74|P-value:3.37E-4 ID:SGTC_3292|Compound:9120825|FD-Score:3.55|P-value:6.29E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:3.83|P-value:2.55E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.52|P-value:9.88E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:3.39|P-value:9.99E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:6.22|P-value:9.67E-9 ID:SGTC_168|Compound:3970-0862|FD-Score:8.23|P-value:6.38E-14 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.50|P-value:7.14E-4 ID:SGTC_262|Compound:fenpropimorph|FD-Score:5.64|P-value:1.71E-7 ID:SGTC_312|Compound:4092-0653|FD-Score:5.46|P-value:3.98E-7 ID:SGTC_349|Compound:k015-0014|FD-Score:3.92|P-value:1.88E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.53|P-value:6.54E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:3.47|P-value:7.89E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.75|P-value:1.38E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:-4.94|P-value:6.50E-6 ID:SGTC_515|Compound:4092-1064|FD-Score:4.45|P-value:2.78E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:12.10|P-value:8.28E-28 ID:SGTC_562|Compound:3772-2733|FD-Score:6.54|P-value:1.76E-9 ID:SGTC_658|Compound:3253-0951|FD-Score:4.85|P-value:5.68E-6 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.73|P-value:5.29E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:5.56|P-value:2.46E-7 ID:SGTC_690|Compound:glyoxal|FD-Score:7.33|P-value:1.93E-11 ID:SGTC_717|Compound:1532-0246|FD-Score:4.19|P-value:7.18E-5 ID:SGTC_727|Compound:3032-0951|FD-Score:4.69|P-value:1.06E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:5.19|P-value:1.34E-6 ID:SGTC_790|Compound:1575-0012|FD-Score:5.26|P-value:9.98E-7 ID:SGTC_919|Compound:tnp00227|FD-Score:3.42|P-value:9.17E-4 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:3.49|P-value:7.46E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:3.57|P-value:5.92E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:-4.61|P-value:2.40E-5 ID:SGTC_984|Compound:1492-0255|FD-Score:-4.34|P-value:6.69E-5 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:4.74|P-value:8.91E-6 ID:SGTC_1108|Compound:betulinic acid|FD-Score:3.46|P-value:8.26E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:4.28|P-value:5.24E-5 ID:SGTC_1166|Compound:k206-0078|FD-Score:-4.05|P-value:1.81E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:4.80|P-value:6.95E-6 ID:SGTC_1180|Compound:1193-0155|FD-Score:4.33|P-value:4.24E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:5.20|P-value:1.28E-6 ID:SGTC_1422|Compound:4031-0024|FD-Score:5.27|P-value:9.36E-7 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:9.22|P-value:5.12E-17 ID:SGTC_1712|Compound:st032288|FD-Score:5.85|P-value:6.43E-8 ID:SGTC_1715|Compound:st032160|FD-Score:3.58|P-value:5.74E-4 ID:SGTC_1800|Compound:st050079|FD-Score:4.48|P-value:2.47E-5 ID:SGTC_1803|Compound:st050068|FD-Score:3.87|P-value:2.20E-4 ID:SGTC_1847|Compound:st056231|FD-Score:3.54|P-value:6.47E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:3.61|P-value:5.10E-4 ID:SGTC_1875|Compound:nocodazole|FD-Score:6.59|P-value:1.35E-9 ID:SGTC_2038|Compound:4100038|FD-Score:3.99|P-value:1.45E-4 ID:SGTC_2215|Compound:7188065|FD-Score:4.86|P-value:5.43E-6 ID:SGTC_2233|Compound:sulbentine|FD-Score:4.02|P-value:1.29E-4 ID:SGTC_2237|Compound:6667747|FD-Score:4.37|P-value:3.70E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:6.92|P-value:2.15E-10 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.10|P-value:1.99E-6 ID:SGTC_2395|Compound:5646831|FD-Score:5.89|P-value:5.13E-8 ID:SGTC_2468|Compound:5987765|FD-Score:3.65|P-value:4.47E-4 ID:SGTC_2476|Compound:5806916|FD-Score:3.66|P-value:4.37E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:5.18|P-value:1.36E-6 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.54|P-value:6.43E-4 ID:SGTC_2857|Compound:9024691|FD-Score:4.19|P-value:7.08E-5 ID:SGTC_2866|Compound:9034245|FD-Score:3.48|P-value:7.62E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.59|P-value:5.52E-4 ID:SGTC_3071|Compound:9117417|FD-Score:3.74|P-value:3.37E-4 ID:SGTC_3292|Compound:9120825|FD-Score:3.55|P-value:6.29E-4

Top fitness defect scores for YOL081W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_528 3492-0019 12.10 172.0 µM 8.28E-28 calcium & mitochondrial duress 30 5.67E-34
2 SGTC_1597 7-benzyloxygramine 9.22 47.5 µM 5.12E-17 calcium & mitochondrial duress 17 1.42E-20
3 SGTC_168 3970-0862 8.23 530.5 µM 6.38E-14 19 9.73E-17
4 SGTC_690 glyoxal 7.33 20.3 µM 1.93E-11 32 1.14E-13
5 SGTC_2249 haloperidol 6.92 116.4 µM 2.15E-10 ergosterol depletion effects on membrane 33 2.25E-12
6 SGTC_1875 nocodazole 6.59 6.0 µM 1.35E-9 tubulin folding & SWR complex 24 2.16E-11
7 SGTC_562 3772-2733 6.54 112.0 µM 1.76E-9 ERG2 30 3.00E-11
8 SGTC_150 3970-0969 6.22 77.2 µM 9.67E-9 18 2.45E-10
9 SGTC_2395 5646831 5.89 180.2 µM 5.13E-8 7 1.92E-9
10 SGTC_1712 st032288 5.85 53.4 µM 6.43E-8 calcium & mitochondrial duress 82 2.53E-9
11 SGTC_262 fenpropimorph 5.64 62.5 µM 1.71E-7 ergosterol depletion effects on membrane 15 8.46E-9
12 SGTC_676 falcarinol 5.56 140.0 µM 2.46E-7 19 1.32E-8
13 SGTC_312 4092-0653 5.46 6.8 µM 3.98E-7 ergosterol depletion effects on membrane 44 2.39E-8
14 SGTC_1422 4031-0024 5.27 15.5 µM 9.36E-7 iron homeostasis 5 6.82E-8
15 SGTC_790 1575-0012 5.26 79.1 µM 9.98E-7 TSC3-RPN4 10 7.37E-8
16 SGTC_1263 0774-1208 5.20 64.8 µM 1.28E-6 19 1.00E-7
17 SGTC_778 3456-2434 5.19 196.0 µM 1.34E-6 mitochondrial response to ROS 38 1.06E-7
18 SGTC_2531 methyl deoxycholate 5.18 70.1 µM 1.36E-6 10 1.08E-7
19 SGTC_2250 alverine citrate 5.10 130.0 µM 1.99E-6 91 1.72E-7
20 SGTC_2215 7188065 4.86 200.0 µM 5.43E-6 2 5.90E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.336 2.39E-89 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.312 9.71E-77 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.254 2.43E-50 YHR205W SCH9 hom AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
0.244 2.06E-46 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.243 2.97E-46 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.241 1.85E-45 YJR088C EMC2 hom Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35
0.233 1.18E-42 YFL005W SEC4 het Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
0.231 5.47E-42 YJL164C TPK1 hom cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
0.221 3.27E-38 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.218 2.32E-37 YBL051C PIN4 hom Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
0.207 1.02E-33 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.206 1.84E-33 YLR404W FLD1 hom Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy
0.205 4.01E-33 YDL023C_d YDL023C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance
0.201 5.41E-32 YGL035C MIG1 hom Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion
0.197 1.17E-30 YDR093W DNF2 hom Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase