YOL086W-A / MHF1

Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL086W-A / MHF1

Click on Significant Values for Screen Details ID:SGTC_164|Compound:4100-3994|FD-Score:3.60|P-value:1.51E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:3.43|P-value:3.39E-5 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.06|P-value:1.95E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.37|P-value:4.58E-5 ID:SGTC_269|Compound:exalamide|FD-Score:2.79|P-value:6.12E-4 ID:SGTC_443|Compound:clozapine|FD-Score:3.57|P-value:1.69E-5 ID:SGTC_447|Compound:ml7|FD-Score:-2.75|P-value:6.85E-4 ID:SGTC_448|Compound:monastrol|FD-Score:-5.70|P-value:1.75E-11 ID:SGTC_460|Compound:trequinsin|FD-Score:-5.39|P-value:1.97E-10 ID:SGTC_461|Compound:N-acetylfarnesylcysteine|FD-Score:-5.42|P-value:1.49E-10 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.22|P-value:9.23E-5 ID:SGTC_516|Compound:3992-2719|FD-Score:-4.23|P-value:4.37E-7 ID:SGTC_521|Compound:r062-0005|FD-Score:-4.02|P-value:1.50E-6 ID:SGTC_522|Compound:1273-0010|FD-Score:6.99|P-value:2.57E-16 ID:SGTC_531|Compound:3788-1495|FD-Score:-3.07|P-value:1.78E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:5.26|P-value:5.27E-10 ID:SGTC_537|Compound:1334-0398|FD-Score:4.17|P-value:6.76E-7 ID:SGTC_561|Compound:1550-0330|FD-Score:3.55|P-value:1.91E-5 ID:SGTC_618|Compound:k072-0232|FD-Score:2.83|P-value:5.11E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:3.48|P-value:2.75E-5 ID:SGTC_738|Compound:1315-0003|FD-Score:2.73|P-value:7.73E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-2.71|P-value:8.21E-4 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.17|P-value:1.16E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.88|P-value:3.35E-6 ID:SGTC_1210|Compound:3013-0140|FD-Score:2.99|P-value:2.57E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:2.71|P-value:8.31E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:-3.15|P-value:1.26E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:2.91|P-value:3.75E-4 ID:SGTC_1314|Compound:1222-0010|FD-Score:2.70|P-value:8.63E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.04|P-value:2.07E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:-4.56|P-value:5.77E-8 ID:SGTC_1376|Compound:2910-0861|FD-Score:-3.10|P-value:1.58E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:2.84|P-value:4.97E-4 ID:SGTC_1559|Compound:coumarin 343|FD-Score:2.78|P-value:6.34E-4 ID:SGTC_1635|Compound:st007035|FD-Score:2.80|P-value:5.89E-4 ID:SGTC_1731|Compound:st035964|FD-Score:2.72|P-value:8.18E-4 ID:SGTC_1794|Compound:st048918|FD-Score:2.73|P-value:7.77E-4 ID:SGTC_1796|Compound:st049173|FD-Score:3.04|P-value:2.14E-4 ID:SGTC_1949|Compound:st076024|FD-Score:-3.46|P-value:2.96E-5 ID:SGTC_1964|Compound:st076914|FD-Score:-2.95|P-value:3.06E-4 ID:SGTC_1966|Compound:st070145|FD-Score:-3.60|P-value:1.46E-5 ID:SGTC_1967|Compound:st070177|FD-Score:4.38|P-value:1.92E-7 ID:SGTC_1971|Compound:st069429|FD-Score:3.90|P-value:3.08E-6 ID:SGTC_2132|Compound:5317594|FD-Score:-2.88|P-value:4.03E-4 ID:SGTC_2133|Compound:5320837|FD-Score:-3.25|P-value:8.07E-5 ID:SGTC_2296|Compound:7971656|FD-Score:4.67|P-value:3.11E-8 ID:SGTC_2298|Compound:7976326|FD-Score:3.62|P-value:1.37E-5 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-3.47|P-value:2.85E-5 ID:SGTC_2925|Compound:9010536|FD-Score:-3.16|P-value:1.23E-4 ID:SGTC_3106|Compound:9121286|FD-Score:2.87|P-value:4.32E-4 ID:SGTC_3182|Compound:9108849|FD-Score:-2.86|P-value:4.48E-4 ID:SGTC_3249|Compound:9135183|FD-Score:2.82|P-value:5.36E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:3.60|P-value:1.51E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:3.43|P-value:3.39E-5 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.06|P-value:1.95E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.37|P-value:4.58E-5 ID:SGTC_269|Compound:exalamide|FD-Score:2.79|P-value:6.12E-4 ID:SGTC_443|Compound:clozapine|FD-Score:3.57|P-value:1.69E-5 ID:SGTC_447|Compound:ml7|FD-Score:-2.75|P-value:6.85E-4 ID:SGTC_448|Compound:monastrol|FD-Score:-5.70|P-value:1.75E-11 ID:SGTC_460|Compound:trequinsin|FD-Score:-5.39|P-value:1.97E-10 ID:SGTC_461|Compound:N-acetylfarnesylcysteine|FD-Score:-5.42|P-value:1.49E-10 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.22|P-value:9.23E-5 ID:SGTC_516|Compound:3992-2719|FD-Score:-4.23|P-value:4.37E-7 ID:SGTC_521|Compound:r062-0005|FD-Score:-4.02|P-value:1.50E-6 ID:SGTC_522|Compound:1273-0010|FD-Score:6.99|P-value:2.57E-16 ID:SGTC_531|Compound:3788-1495|FD-Score:-3.07|P-value:1.78E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:5.26|P-value:5.27E-10 ID:SGTC_537|Compound:1334-0398|FD-Score:4.17|P-value:6.76E-7 ID:SGTC_561|Compound:1550-0330|FD-Score:3.55|P-value:1.91E-5 ID:SGTC_618|Compound:k072-0232|FD-Score:2.83|P-value:5.11E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:3.48|P-value:2.75E-5 ID:SGTC_738|Compound:1315-0003|FD-Score:2.73|P-value:7.73E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-2.71|P-value:8.21E-4 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.17|P-value:1.16E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.88|P-value:3.35E-6 ID:SGTC_1210|Compound:3013-0140|FD-Score:2.99|P-value:2.57E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:2.71|P-value:8.31E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:-3.15|P-value:1.26E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:2.91|P-value:3.75E-4 ID:SGTC_1314|Compound:1222-0010|FD-Score:2.70|P-value:8.63E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.04|P-value:2.07E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:-4.56|P-value:5.77E-8 ID:SGTC_1376|Compound:2910-0861|FD-Score:-3.10|P-value:1.58E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:2.84|P-value:4.97E-4 ID:SGTC_1559|Compound:coumarin 343|FD-Score:2.78|P-value:6.34E-4 ID:SGTC_1635|Compound:st007035|FD-Score:2.80|P-value:5.89E-4 ID:SGTC_1731|Compound:st035964|FD-Score:2.72|P-value:8.18E-4 ID:SGTC_1794|Compound:st048918|FD-Score:2.73|P-value:7.77E-4 ID:SGTC_1796|Compound:st049173|FD-Score:3.04|P-value:2.14E-4 ID:SGTC_1949|Compound:st076024|FD-Score:-3.46|P-value:2.96E-5 ID:SGTC_1964|Compound:st076914|FD-Score:-2.95|P-value:3.06E-4 ID:SGTC_1966|Compound:st070145|FD-Score:-3.60|P-value:1.46E-5 ID:SGTC_1967|Compound:st070177|FD-Score:4.38|P-value:1.92E-7 ID:SGTC_1971|Compound:st069429|FD-Score:3.90|P-value:3.08E-6 ID:SGTC_2132|Compound:5317594|FD-Score:-2.88|P-value:4.03E-4 ID:SGTC_2133|Compound:5320837|FD-Score:-3.25|P-value:8.07E-5 ID:SGTC_2296|Compound:7971656|FD-Score:4.67|P-value:3.11E-8 ID:SGTC_2298|Compound:7976326|FD-Score:3.62|P-value:1.37E-5 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-3.47|P-value:2.85E-5 ID:SGTC_2925|Compound:9010536|FD-Score:-3.16|P-value:1.23E-4 ID:SGTC_3106|Compound:9121286|FD-Score:2.87|P-value:4.32E-4 ID:SGTC_3182|Compound:9108849|FD-Score:-2.86|P-value:4.48E-4 ID:SGTC_3249|Compound:9135183|FD-Score:2.82|P-value:5.36E-4

Top fitness defect scores for YOL086W-A deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_522 1273-0010 6.99 11.6 µM 2.57E-16 60S ribosome export 1 1.40E-12
2 SGTC_535 2293-4129 5.26 48.2 µM 5.27E-10 NEO1 31 7.26E-8
3 SGTC_2296 7971656 4.67 126.5 µM 3.11E-8 28 1.54E-6
4 SGTC_1967 st070177 4.38 51.4 µM 1.92E-7 10 6.03E-6
5 SGTC_537 1334-0398 4.17 209.0 µM 6.76E-7 19 1.55E-5
6 SGTC_1971 st069429 3.90 63.5 µM 3.08E-6 60S ribosome export 16 4.87E-5
7 SGTC_1129 3453-1384 3.88 223.0 µM 3.35E-6 40 5.18E-5
8 SGTC_2298 7976326 3.62 193.8 µM 1.37E-5 17 1.50E-4
9 SGTC_164 4100-3994 3.60 89.6 µM 1.51E-5 44 1.61E-4
10 SGTC_443 clozapine 3.57 122.0 µM 1.69E-5 NEO1-PIK1 16 1.76E-4
11 SGTC_561 1550-0330 3.55 72.9 µM 1.91E-5 17 1.93E-4
12 SGTC_700 3453-2283 3.48 142.0 µM 2.75E-5 amide catabolism 47 2.53E-4
13 SGTC_236 1509-0011 3.43 5.3 µM 3.39E-5 424 2.97E-4
14 SGTC_268 camptothecin 3.37 39.6 µM 4.58E-5 DNA damage response 108 3.72E-4
15 SGTC_464 bw-b 70c 3.22 158.0 µM 9.23E-5 54 6.32E-4
16 SGTC_1117 retinoic acid 3.17 1.1 µM 1.16E-4 43 7.51E-4
17 SGTC_243 5hr heat shock (37°C) + splitomicin 3.06 1.0 µM 1.95E-4 heat shock/prefoldin 101 0.00111
18 SGTC_1368 1852-0141 3.04 284.0 µM 2.07E-4 ERG2 76 0.00117
19 SGTC_1796 st049173 3.04 110.4 µM 2.14E-4 31 0.00119
20 SGTC_1210 3013-0140 2.99 750.0 nM 2.57E-4 37 0.00137

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.119 4.50E-12 YKR106W GEX2 hom Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway
0.118 7.56E-12 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.116 1.35E-11 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.113 5.48E-11 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.111 1.35E-10 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.110 1.61E-10 YDR518W EUG1 hom Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.109 2.76E-10 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.106 8.66E-10 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.105 1.25E-9 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.103 2.29E-9 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.102 3.16E-9 YHR116W COX23 hom Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
0.101 5.61E-9 YMR286W MRPL33 hom Mitochondrial ribosomal protein of the large subunit
0.100 6.14E-9 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.100 6.51E-9 YCL025C AGP1 hom Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication
0.099 8.55E-9 YBR221W-A_p YBR221W-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry