YOL087C / DUF1

Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL087C / DUF1

Click on Significant Values for Screen Details ID:SGTC_122|Compound:0236-0016|FD-Score:5.40|P-value:4.29E-6 ID:SGTC_133|Compound:0724-0033|FD-Score:4.25|P-value:2.50E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:4.71|P-value:5.42E-5 ID:SGTC_432|Compound:NS 1619|FD-Score:-4.76|P-value:2.13E-5 ID:SGTC_440|Compound:fluspirilene|FD-Score:-4.35|P-value:8.81E-5 ID:SGTC_526|Compound:4469-0568|FD-Score:4.39|P-value:1.56E-4 ID:SGTC_556|Compound:4058-0063|FD-Score:3.96|P-value:5.92E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:-3.60|P-value:9.02E-4 ID:SGTC_810|Compound:3825-7150|FD-Score:-5.16|P-value:4.66E-6 ID:SGTC_1194|Compound:1488-0194|FD-Score:4.18|P-value:3.08E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:4.01|P-value:5.20E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:3.81|P-value:9.23E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.59|P-value:9.45E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:4.25|P-value:2.48E-4 ID:SGTC_1608|Compound:st001473|FD-Score:-4.44|P-value:6.60E-5 ID:SGTC_1612|Compound:st002233|FD-Score:4.26|P-value:2.39E-4 ID:SGTC_1613|Compound:st002381|FD-Score:5.66|P-value:1.48E-6 ID:SGTC_1623|Compound:st003709|FD-Score:4.98|P-value:2.05E-5 ID:SGTC_1627|Compound:st003713|FD-Score:5.48|P-value:3.16E-6 ID:SGTC_1635|Compound:st007035|FD-Score:-3.79|P-value:5.15E-4 ID:SGTC_1889|Compound:st059080|FD-Score:-5.00|P-value:8.68E-6 ID:SGTC_2049|Compound:5262831|FD-Score:4.33|P-value:1.89E-4 ID:SGTC_2063|Compound:5246145|FD-Score:4.44|P-value:1.34E-4 ID:SGTC_2071|Compound:5230951|FD-Score:5.09|P-value:1.38E-5 ID:SGTC_2075|Compound:5212009|FD-Score:-3.78|P-value:5.32E-4 ID:SGTC_2082|Compound:5219693|FD-Score:9.00|P-value:3.71E-14 ID:SGTC_2092|Compound:5303694|FD-Score:5.23|P-value:8.21E-6 ID:SGTC_2173|Compound:5763948|FD-Score:4.17|P-value:3.21E-4 ID:SGTC_2184|Compound:6075246|FD-Score:5.99|P-value:3.74E-7 ID:SGTC_2266|Compound:7929956|FD-Score:3.96|P-value:6.02E-4 ID:SGTC_2367|Compound:9070478|FD-Score:4.21|P-value:2.81E-4 ID:SGTC_2368|Compound:9071271|FD-Score:5.06|P-value:1.55E-5 ID:SGTC_2440|Compound:5628896|FD-Score:3.81|P-value:9.27E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:-4.35|P-value:8.83E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.38|P-value:1.64E-4 ID:SGTC_2857|Compound:9024691|FD-Score:6.46|P-value:4.66E-8 ID:SGTC_2902|Compound:5787006|FD-Score:4.52|P-value:1.05E-4 ID:SGTC_2924|Compound:9010168|FD-Score:4.32|P-value:1.97E-4 ID:SGTC_2970|Compound:9090938|FD-Score:4.02|P-value:5.00E-4 ID:SGTC_2995|Compound:9067767|FD-Score:4.19|P-value:2.99E-4 ID:SGTC_3025|Compound:9087895|FD-Score:5.25|P-value:7.60E-6 ID:SGTC_3150|Compound:9097574|FD-Score:4.70|P-value:5.67E-5 ID:SGTC_3174|Compound:9107204|FD-Score:3.80|P-value:9.36E-4 ID:SGTC_3216|Compound:9129716|FD-Score:8.41|P-value:1.42E-12 ID:SGTC_3219|Compound:9131112|FD-Score:6.03|P-value:3.15E-7 ID:SGTC_3264|Compound:9137442|FD-Score:4.20|P-value:2.90E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:5.40|P-value:4.29E-6 ID:SGTC_133|Compound:0724-0033|FD-Score:4.25|P-value:2.50E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:4.71|P-value:5.42E-5 ID:SGTC_432|Compound:NS 1619|FD-Score:-4.76|P-value:2.13E-5 ID:SGTC_440|Compound:fluspirilene|FD-Score:-4.35|P-value:8.81E-5 ID:SGTC_526|Compound:4469-0568|FD-Score:4.39|P-value:1.56E-4 ID:SGTC_556|Compound:4058-0063|FD-Score:3.96|P-value:5.92E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:-3.60|P-value:9.02E-4 ID:SGTC_810|Compound:3825-7150|FD-Score:-5.16|P-value:4.66E-6 ID:SGTC_1194|Compound:1488-0194|FD-Score:4.18|P-value:3.08E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:4.01|P-value:5.20E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:3.81|P-value:9.23E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.59|P-value:9.45E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:4.25|P-value:2.48E-4 ID:SGTC_1608|Compound:st001473|FD-Score:-4.44|P-value:6.60E-5 ID:SGTC_1612|Compound:st002233|FD-Score:4.26|P-value:2.39E-4 ID:SGTC_1613|Compound:st002381|FD-Score:5.66|P-value:1.48E-6 ID:SGTC_1623|Compound:st003709|FD-Score:4.98|P-value:2.05E-5 ID:SGTC_1627|Compound:st003713|FD-Score:5.48|P-value:3.16E-6 ID:SGTC_1635|Compound:st007035|FD-Score:-3.79|P-value:5.15E-4 ID:SGTC_1889|Compound:st059080|FD-Score:-5.00|P-value:8.68E-6 ID:SGTC_2049|Compound:5262831|FD-Score:4.33|P-value:1.89E-4 ID:SGTC_2063|Compound:5246145|FD-Score:4.44|P-value:1.34E-4 ID:SGTC_2071|Compound:5230951|FD-Score:5.09|P-value:1.38E-5 ID:SGTC_2075|Compound:5212009|FD-Score:-3.78|P-value:5.32E-4 ID:SGTC_2082|Compound:5219693|FD-Score:9.00|P-value:3.71E-14 ID:SGTC_2092|Compound:5303694|FD-Score:5.23|P-value:8.21E-6 ID:SGTC_2173|Compound:5763948|FD-Score:4.17|P-value:3.21E-4 ID:SGTC_2184|Compound:6075246|FD-Score:5.99|P-value:3.74E-7 ID:SGTC_2266|Compound:7929956|FD-Score:3.96|P-value:6.02E-4 ID:SGTC_2367|Compound:9070478|FD-Score:4.21|P-value:2.81E-4 ID:SGTC_2368|Compound:9071271|FD-Score:5.06|P-value:1.55E-5 ID:SGTC_2440|Compound:5628896|FD-Score:3.81|P-value:9.27E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:-4.35|P-value:8.83E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.38|P-value:1.64E-4 ID:SGTC_2857|Compound:9024691|FD-Score:6.46|P-value:4.66E-8 ID:SGTC_2902|Compound:5787006|FD-Score:4.52|P-value:1.05E-4 ID:SGTC_2924|Compound:9010168|FD-Score:4.32|P-value:1.97E-4 ID:SGTC_2970|Compound:9090938|FD-Score:4.02|P-value:5.00E-4 ID:SGTC_2995|Compound:9067767|FD-Score:4.19|P-value:2.99E-4 ID:SGTC_3025|Compound:9087895|FD-Score:5.25|P-value:7.60E-6 ID:SGTC_3150|Compound:9097574|FD-Score:4.70|P-value:5.67E-5 ID:SGTC_3174|Compound:9107204|FD-Score:3.80|P-value:9.36E-4 ID:SGTC_3216|Compound:9129716|FD-Score:8.41|P-value:1.42E-12 ID:SGTC_3219|Compound:9131112|FD-Score:6.03|P-value:3.15E-7 ID:SGTC_3264|Compound:9137442|FD-Score:4.20|P-value:2.90E-4

Top fitness defect scores for YOL087C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2082 5219693 9.00 150.0 µM 3.71E-14 RNA processing & uracil transport 2 1.13E-19
2 SGTC_3216 9129716 8.41 49.5 µM 1.42E-12 RPP1 & pyrimidine depletion 1 2.01E-17
3 SGTC_2857 9024691 6.46 58.4 µM 4.66E-8 2 5.40E-11
4 SGTC_3219 9131112 6.03 49.5 µM 3.15E-7 Golgi 15 8.19E-10
5 SGTC_2184 6075246 5.99 200.0 µM 3.74E-7 2 1.04E-9
6 SGTC_1613 st002381 5.66 52.1 µM 1.48E-6 6 7.39E-9
7 SGTC_1627 st003713 5.48 25.6 µM 3.16E-6 calcium & mitochondrial duress 35 2.18E-8
8 SGTC_122 0236-0016 5.40 402.4 µM 4.29E-6 RNA processing & uracil transport 11 3.37E-8
9 SGTC_3025 9087895 5.25 49.5 µM 7.60E-6 PDR1 24 7.60E-8
10 SGTC_2092 5303694 5.23 80.7 µM 8.21E-6 TSC3-RPN4 10 8.48E-8
11 SGTC_2071 5230951 5.09 39.9 µM 1.38E-5 TSC3-RPN4 5 1.78E-7
12 SGTC_2368 9071271 5.06 200.0 µM 1.55E-5 10 2.09E-7
13 SGTC_1623 st003709 4.98 20.3 µM 2.05E-5 calcium & mitochondrial duress 32 3.11E-7
14 SGTC_354 2783-3500 4.71 4.9 µM 5.42E-5 cell wall 33 1.24E-6
15 SGTC_3150 9097574 4.70 49.5 µM 5.67E-5 6 1.32E-6
16 SGTC_2902 5787006 4.52 60.5 µM 1.05E-4 Golgi 17 3.16E-6
17 SGTC_2063 5246145 4.44 200.0 µM 1.34E-4 15 4.48E-6
18 SGTC_526 4469-0568 4.39 170.0 µM 1.56E-4 azole & statin 28 5.55E-6
19 SGTC_2688 kanamycin b 4.38 96.7 µM 1.64E-4 calcium & mitochondrial duress 313 5.97E-6
20 SGTC_2049 5262831 4.33 67.2 µM 1.89E-4 19 7.30E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.111 9.92E-11 YBR021W FUR4 hom Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
0.108 3.60E-10 YGL215W CLG1 hom Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2
0.108 3.69E-10 YBL022C PIM1 hom ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
0.103 1.89E-9 YBL067C UBP13 hom Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication
0.103 2.36E-9 YGL244W RTF1 hom Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
0.101 4.52E-9 YAL038W CDC19 het Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
0.100 6.53E-9 YDR293C SSD1 hom Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
0.099 8.62E-9 YKR031C SPO14 hom Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions
0.099 1.05E-8 YDR290W_d YDR290W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103
0.088 2.93E-7 YLR015W BRE2 hom Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L
0.087 4.56E-7 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.083 1.49E-6 YGR285C ZUO1 hom Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
0.083 1.71E-6 YDR313C PIB1 hom RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain
0.081 2.82E-6 YJR059W PTK2 hom Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake
0.080 3.19E-6 YDR469W SDC1 hom Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30