YOL090W / MSH2

Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL090W / MSH2

Click on Significant Values for Screen Details ID:SGTC_25|Compound:0118-0223|FD-Score:-4.92|P-value:1.01E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.49|P-value:3.69E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:3.46|P-value:4.31E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:4.26|P-value:2.05E-5 ID:SGTC_124|Compound:0136-0217|FD-Score:3.37|P-value:5.81E-4 ID:SGTC_125|Compound:0133-0007|FD-Score:3.54|P-value:3.28E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:-4.62|P-value:4.06E-6 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.21|P-value:9.88E-4 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:3.26|P-value:8.52E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:3.37|P-value:5.83E-4 ID:SGTC_364|Compound:0083-0097|FD-Score:3.29|P-value:7.70E-4 ID:SGTC_368|Compound:fentichlor|FD-Score:4.32|P-value:1.56E-5 ID:SGTC_610|Compound:4215-0184|FD-Score:4.09|P-value:4.12E-5 ID:SGTC_795|Compound:0685-0265|FD-Score:3.40|P-value:5.27E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:3.56|P-value:3.05E-4 ID:SGTC_1024|Compound:4507-0395|FD-Score:5.11|P-value:4.38E-7 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.26|P-value:8.57E-4 ID:SGTC_1058|Compound:3448-5425|FD-Score:-3.45|P-value:4.30E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:4.43|P-value:9.75E-6 ID:SGTC_1205|Compound:4161-2785|FD-Score:-3.45|P-value:4.30E-4 ID:SGTC_1319|Compound:1306-0155|FD-Score:3.39|P-value:5.53E-4 ID:SGTC_1727|Compound:st034309|FD-Score:5.66|P-value:2.48E-8 ID:SGTC_1771|Compound:st048882|FD-Score:3.79|P-value:1.32E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.70|P-value:1.83E-4 ID:SGTC_1788|Compound:fluconazole|FD-Score:3.86|P-value:1.02E-4 ID:SGTC_1794|Compound:st048918|FD-Score:-4.59|P-value:4.70E-6 ID:SGTC_1799|Compound:st045822|FD-Score:-3.74|P-value:1.49E-4 ID:SGTC_1807|Compound:st050092|FD-Score:3.41|P-value:5.08E-4 ID:SGTC_1837|Compound:st055472|FD-Score:-3.54|P-value:3.14E-4 ID:SGTC_1904|Compound:st060225|FD-Score:6.94|P-value:1.14E-11 ID:SGTC_2031|Compound:5180455|FD-Score:4.93|P-value:1.02E-6 ID:SGTC_2373|Compound:9071805|FD-Score:3.83|P-value:1.14E-4 ID:SGTC_2470|Compound:5664817|FD-Score:3.38|P-value:5.64E-4 ID:SGTC_2505|Compound:galangin|FD-Score:-3.42|P-value:4.78E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:4.59|P-value:4.84E-6 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.58|P-value:2.80E-4 ID:SGTC_2834|Compound:9001755|FD-Score:4.12|P-value:3.61E-5 ID:SGTC_2864|Compound:9034755|FD-Score:3.54|P-value:3.24E-4 ID:SGTC_3068|Compound:9116768|FD-Score:-3.31|P-value:6.99E-4 ID:SGTC_3074|Compound:9116018|FD-Score:3.53|P-value:3.38E-4 ID:SGTC_3086|Compound:9117350|FD-Score:-3.47|P-value:4.02E-4 ID:SGTC_25|Compound:0118-0223|FD-Score:-4.92|P-value:1.01E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.49|P-value:3.69E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:3.46|P-value:4.31E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:4.26|P-value:2.05E-5 ID:SGTC_124|Compound:0136-0217|FD-Score:3.37|P-value:5.81E-4 ID:SGTC_125|Compound:0133-0007|FD-Score:3.54|P-value:3.28E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:-4.62|P-value:4.06E-6 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.21|P-value:9.88E-4 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:3.26|P-value:8.52E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:3.37|P-value:5.83E-4 ID:SGTC_364|Compound:0083-0097|FD-Score:3.29|P-value:7.70E-4 ID:SGTC_368|Compound:fentichlor|FD-Score:4.32|P-value:1.56E-5 ID:SGTC_610|Compound:4215-0184|FD-Score:4.09|P-value:4.12E-5 ID:SGTC_795|Compound:0685-0265|FD-Score:3.40|P-value:5.27E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:3.56|P-value:3.05E-4 ID:SGTC_1024|Compound:4507-0395|FD-Score:5.11|P-value:4.38E-7 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.26|P-value:8.57E-4 ID:SGTC_1058|Compound:3448-5425|FD-Score:-3.45|P-value:4.30E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:4.43|P-value:9.75E-6 ID:SGTC_1205|Compound:4161-2785|FD-Score:-3.45|P-value:4.30E-4 ID:SGTC_1319|Compound:1306-0155|FD-Score:3.39|P-value:5.53E-4 ID:SGTC_1727|Compound:st034309|FD-Score:5.66|P-value:2.48E-8 ID:SGTC_1771|Compound:st048882|FD-Score:3.79|P-value:1.32E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.70|P-value:1.83E-4 ID:SGTC_1788|Compound:fluconazole|FD-Score:3.86|P-value:1.02E-4 ID:SGTC_1794|Compound:st048918|FD-Score:-4.59|P-value:4.70E-6 ID:SGTC_1799|Compound:st045822|FD-Score:-3.74|P-value:1.49E-4 ID:SGTC_1807|Compound:st050092|FD-Score:3.41|P-value:5.08E-4 ID:SGTC_1837|Compound:st055472|FD-Score:-3.54|P-value:3.14E-4 ID:SGTC_1904|Compound:st060225|FD-Score:6.94|P-value:1.14E-11 ID:SGTC_2031|Compound:5180455|FD-Score:4.93|P-value:1.02E-6 ID:SGTC_2373|Compound:9071805|FD-Score:3.83|P-value:1.14E-4 ID:SGTC_2470|Compound:5664817|FD-Score:3.38|P-value:5.64E-4 ID:SGTC_2505|Compound:galangin|FD-Score:-3.42|P-value:4.78E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:4.59|P-value:4.84E-6 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.58|P-value:2.80E-4 ID:SGTC_2834|Compound:9001755|FD-Score:4.12|P-value:3.61E-5 ID:SGTC_2864|Compound:9034755|FD-Score:3.54|P-value:3.24E-4 ID:SGTC_3068|Compound:9116768|FD-Score:-3.31|P-value:6.99E-4 ID:SGTC_3074|Compound:9116018|FD-Score:3.53|P-value:3.38E-4 ID:SGTC_3086|Compound:9117350|FD-Score:-3.47|P-value:4.02E-4

Top fitness defect scores for YOL090W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1904 st060225 6.94 78.0 µM 1.14E-11 1 1.95E-12
2 SGTC_1727 st034309 5.66 9.9 µM 2.48E-8 10 7.53E-9
3 SGTC_1024 4507-0395 5.11 26.0 µM 4.38E-7 TSC3-RPN4 9 1.65E-7
4 SGTC_2031 5180455 4.93 200.0 µM 1.02E-6 6 4.07E-7
5 SGTC_2540 epiandrosterone 4.59 100.0 µM 4.84E-6 39 2.17E-6
6 SGTC_1118 chelidonine 4.43 707.5 nM 9.75E-6 12 4.60E-6
7 SGTC_368 fentichlor 4.32 4.6 µM 1.56E-5 21 7.64E-6
8 SGTC_117 lucigenin 4.26 22.1 µM 2.05E-5 redox potentiating 73 1.02E-5
9 SGTC_2834 9001755 4.12 58.4 µM 3.61E-5 29 1.88E-5
10 SGTC_610 4215-0184 4.09 155.0 µM 4.12E-5 exosome 10 2.16E-5
11 SGTC_1788 fluconazole 3.86 20.0 µM 1.02E-4 azole & statin 64 5.69E-5
12 SGTC_2373 9071805 3.83 200.0 µM 1.14E-4 14 6.44E-5
13 SGTC_1771 st048882 3.79 4.2 µM 1.32E-4 RPP1 & pyrimidine depletion 25 7.52E-5
14 SGTC_1787 fluconazole 3.70 80.0 µM 1.83E-4 iron homeostasis 34 1.07E-4
15 SGTC_2766 oltipraz 3.58 83.9 µM 2.80E-4 heme requiring 43 1.69E-4
16 SGTC_909 3448-9333 3.56 149.0 µM 3.05E-4 27 1.85E-4
17 SGTC_2864 9034755 3.54 47.6 µM 3.24E-4 19 1.98E-4
18 SGTC_125 0133-0007 3.54 11.8 µM 3.28E-4 DNA intercalators 18 2.00E-4
19 SGTC_3074 9116018 3.53 49.5 µM 3.38E-4 RNA processing & uracil transport 23 2.07E-4
20 SGTC_69 0096-0274 3.46 206.7 µM 4.31E-4 mitochondrial processes 70 2.68E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.123 7.19E-13 YNL152W INN1 het Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis
0.116 1.94E-11 YMR201C RAD14 hom Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
0.103 2.18E-9 YML095C RAD10 hom Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
0.099 1.00E-8 YGR258C RAD2 hom Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
0.093 6.98E-8 YNL326C PFA3 hom Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions
0.088 3.18E-7 YJL217W REE1 hom Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle
0.083 1.40E-6 YPL001W HAT1 hom Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair
0.080 3.98E-6 YOR103C OST2 het Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
0.079 4.91E-6 YPR043W RPL43A hom Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication
0.078 6.14E-6 YDL053C PBP4 hom Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress
0.077 7.59E-6 YER162C RAD4 hom Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins
0.076 9.34E-6 YLL059C_d YLL059C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.076 9.43E-6 YML020W_p YML020W_p hom Putative protein of unknown function
0.074 1.92E-5 YOL158C ENB1 hom Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment
0.073 2.20E-5 YOR069W VPS5 hom Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes