YOL093W / TRM10

tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL093W / TRM10

Click on Significant Values for Screen Details ID:SGTC_77|Compound:0828-0286|FD-Score:4.18|P-value:4.06E-5 ID:SGTC_114|Compound:0364-0001|FD-Score:-3.38|P-value:5.83E-4 ID:SGTC_230|Compound:MPP+|FD-Score:-3.29|P-value:7.88E-4 ID:SGTC_251|Compound:melphalan|FD-Score:3.96|P-value:9.51E-5 ID:SGTC_325|Compound:2810-4341|FD-Score:-3.26|P-value:8.84E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.66|P-value:2.85E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:3.46|P-value:5.76E-4 ID:SGTC_544|Compound:k015-0020|FD-Score:3.34|P-value:8.57E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:3.56|P-value:4.11E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:-3.27|P-value:8.44E-4 ID:SGTC_584|Compound:0986-0246|FD-Score:-3.39|P-value:5.72E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:-3.22|P-value:9.80E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:4.66|P-value:5.58E-6 ID:SGTC_962|Compound:1254-0300|FD-Score:3.69|P-value:2.60E-4 ID:SGTC_1080|Compound:nisoldipine|FD-Score:3.78|P-value:1.86E-4 ID:SGTC_1332|Compound:1480-0653|FD-Score:-3.27|P-value:8.31E-4 ID:SGTC_1476|Compound:4112-3294|FD-Score:-3.25|P-value:8.89E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:6.23|P-value:1.98E-9 ID:SGTC_1648|Compound:st011736|FD-Score:5.20|P-value:4.67E-7 ID:SGTC_1657|Compound:st012939|FD-Score:3.95|P-value:1.02E-4 ID:SGTC_1660|Compound:st012960|FD-Score:3.69|P-value:2.59E-4 ID:SGTC_1728|Compound:st035614|FD-Score:3.59|P-value:3.70E-4 ID:SGTC_1733|Compound:st037357|FD-Score:4.66|P-value:5.67E-6 ID:SGTC_1734|Compound:st037168|FD-Score:3.82|P-value:1.63E-4 ID:SGTC_1853|Compound:st056710|FD-Score:3.49|P-value:5.11E-4 ID:SGTC_1855|Compound:st056195|FD-Score:4.09|P-value:5.96E-5 ID:SGTC_1870|Compound:st057514|FD-Score:6.31|P-value:1.30E-9 ID:SGTC_1871|Compound:st057448|FD-Score:-4.98|P-value:9.54E-7 ID:SGTC_1875|Compound:nocodazole|FD-Score:-4.93|P-value:1.21E-6 ID:SGTC_1914|Compound:st062367|FD-Score:4.58|P-value:8.08E-6 ID:SGTC_1966|Compound:st070145|FD-Score:3.51|P-value:4.88E-4 ID:SGTC_2018|Compound:5133068|FD-Score:4.56|P-value:8.67E-6 ID:SGTC_2119|Compound:5528445|FD-Score:-3.24|P-value:9.28E-4 ID:SGTC_2321|Compound:7390090|FD-Score:4.54|P-value:9.52E-6 ID:SGTC_2326|Compound:6164455|FD-Score:6.57|P-value:2.72E-10 ID:SGTC_2393|Compound:9031399|FD-Score:-5.59|P-value:4.58E-8 ID:SGTC_2402|Compound:5553605|FD-Score:-4.62|P-value:4.98E-6 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.28|P-value:8.10E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:5.37|P-value:2.07E-7 ID:SGTC_2935|Compound:9038016|FD-Score:-3.62|P-value:2.55E-4 ID:SGTC_3016|Compound:9080866|FD-Score:3.74|P-value:2.18E-4 ID:SGTC_3342|Compound:9147791|FD-Score:-3.23|P-value:9.63E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:4.18|P-value:4.06E-5 ID:SGTC_114|Compound:0364-0001|FD-Score:-3.38|P-value:5.83E-4 ID:SGTC_230|Compound:MPP+|FD-Score:-3.29|P-value:7.88E-4 ID:SGTC_251|Compound:melphalan|FD-Score:3.96|P-value:9.51E-5 ID:SGTC_325|Compound:2810-4341|FD-Score:-3.26|P-value:8.84E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.66|P-value:2.85E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:3.46|P-value:5.76E-4 ID:SGTC_544|Compound:k015-0020|FD-Score:3.34|P-value:8.57E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:3.56|P-value:4.11E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:-3.27|P-value:8.44E-4 ID:SGTC_584|Compound:0986-0246|FD-Score:-3.39|P-value:5.72E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:-3.22|P-value:9.80E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:4.66|P-value:5.58E-6 ID:SGTC_962|Compound:1254-0300|FD-Score:3.69|P-value:2.60E-4 ID:SGTC_1080|Compound:nisoldipine|FD-Score:3.78|P-value:1.86E-4 ID:SGTC_1332|Compound:1480-0653|FD-Score:-3.27|P-value:8.31E-4 ID:SGTC_1476|Compound:4112-3294|FD-Score:-3.25|P-value:8.89E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:6.23|P-value:1.98E-9 ID:SGTC_1648|Compound:st011736|FD-Score:5.20|P-value:4.67E-7 ID:SGTC_1657|Compound:st012939|FD-Score:3.95|P-value:1.02E-4 ID:SGTC_1660|Compound:st012960|FD-Score:3.69|P-value:2.59E-4 ID:SGTC_1728|Compound:st035614|FD-Score:3.59|P-value:3.70E-4 ID:SGTC_1733|Compound:st037357|FD-Score:4.66|P-value:5.67E-6 ID:SGTC_1734|Compound:st037168|FD-Score:3.82|P-value:1.63E-4 ID:SGTC_1853|Compound:st056710|FD-Score:3.49|P-value:5.11E-4 ID:SGTC_1855|Compound:st056195|FD-Score:4.09|P-value:5.96E-5 ID:SGTC_1870|Compound:st057514|FD-Score:6.31|P-value:1.30E-9 ID:SGTC_1871|Compound:st057448|FD-Score:-4.98|P-value:9.54E-7 ID:SGTC_1875|Compound:nocodazole|FD-Score:-4.93|P-value:1.21E-6 ID:SGTC_1914|Compound:st062367|FD-Score:4.58|P-value:8.08E-6 ID:SGTC_1966|Compound:st070145|FD-Score:3.51|P-value:4.88E-4 ID:SGTC_2018|Compound:5133068|FD-Score:4.56|P-value:8.67E-6 ID:SGTC_2119|Compound:5528445|FD-Score:-3.24|P-value:9.28E-4 ID:SGTC_2321|Compound:7390090|FD-Score:4.54|P-value:9.52E-6 ID:SGTC_2326|Compound:6164455|FD-Score:6.57|P-value:2.72E-10 ID:SGTC_2393|Compound:9031399|FD-Score:-5.59|P-value:4.58E-8 ID:SGTC_2402|Compound:5553605|FD-Score:-4.62|P-value:4.98E-6 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.28|P-value:8.10E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:5.37|P-value:2.07E-7 ID:SGTC_2935|Compound:9038016|FD-Score:-3.62|P-value:2.55E-4 ID:SGTC_3016|Compound:9080866|FD-Score:3.74|P-value:2.18E-4 ID:SGTC_3342|Compound:9147791|FD-Score:-3.23|P-value:9.63E-4

Top fitness defect scores for YOL093W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2326 6164455 6.57 177.6 µM 2.72E-10 mitochondrial processes 2 2.52E-11
2 SGTC_1870 st057514 6.31 26.5 µM 1.30E-9 RSC & ERG11 3 1.43E-10
3 SGTC_1564 D-Arginine 6.23 115.0 µM 1.98E-9 8 2.29E-10
4 SGTC_2757 clomiphene 5.37 4.2 µM 2.07E-7 fatty acid desaturase (OLE1) 16 4.03E-8
5 SGTC_1648 st011736 5.20 69.8 µM 4.67E-7 PDR1 2 9.97E-8
6 SGTC_927 2425-1185 4.66 123.0 µM 5.58E-6 14 1.57E-6
7 SGTC_1733 st037357 4.66 54.9 µM 5.67E-6 60S ribosome export 12 1.60E-6
8 SGTC_1914 st062367 4.58 72.6 µM 8.08E-6 13 2.37E-6
9 SGTC_2018 5133068 4.56 143.0 µM 8.67E-6 11 2.56E-6
10 SGTC_2321 7390090 4.54 138.8 µM 9.52E-6 17 2.84E-6
11 SGTC_77 0828-0286 4.18 12.5 µM 4.06E-5 TSC3-RPN4 30 1.43E-5
12 SGTC_1855 st056195 4.09 39.6 µM 5.96E-5 60S ribosome export 15 2.19E-5
13 SGTC_251 melphalan 3.96 456.5 µM 9.51E-5 DNA damage response 24 3.68E-5
14 SGTC_1657 st012939 3.95 43.4 µM 1.02E-4 10 3.99E-5
15 SGTC_1734 st037168 3.82 69.4 µM 1.63E-4 28 6.70E-5
16 SGTC_1080 nisoldipine 3.78 24.4 µM 1.86E-4 TSC3-RPN4 32 7.76E-5
17 SGTC_3016 9080866 3.74 49.5 µM 2.18E-4 32 9.25E-5
18 SGTC_1660 st012960 3.69 28.1 µM 2.59E-4 iron homeostasis 48 1.12E-4
19 SGTC_962 1254-0300 3.69 21.5 µM 2.60E-4 32 1.12E-4
20 SGTC_495 decylubiquinone 3.66 155.0 µM 2.85E-4 copper-dependent oxidative stress 35 1.24E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.119 4.61E-12 YNL039W BDP1 het Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs
0.106 7.32E-10 YPL092W SSU1 hom Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein
0.092 8.48E-8 YGL215W CLG1 hom Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2
0.087 4.90E-7 YOL052C-A DDR2 hom Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication
0.086 6.77E-7 YMR026C PEX12 hom C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder
0.083 1.44E-6 YPR022C_p YPR022C_p hom Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
0.082 2.25E-6 YMR086C-A_d YMR086C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.081 2.95E-6 YNR020C ATP23 hom Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p
0.078 5.41E-6 YLR306W UBC12 hom Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes
0.077 7.67E-6 YDR524C AGE1 hom ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif
0.076 1.02E-5 YDR100W TVP15 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p
0.074 2.01E-5 YOR249C APC5 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress
0.072 3.12E-5 YLR211C_p YLR211C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron
0.072 3.13E-5 YML038C YMD8 hom Putative nucleotide sugar transporter, has similarity to Vrg4p
0.069 5.62E-5 YOR124C UBP2 hom Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity