YOL094C / RFC4

Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOL094C / RFC4

Click on Significant Values for Screen Details ID:SGTC_44|Compound:0929-0055|FD-Score:-2.95|P-value:2.84E-4 ID:SGTC_193|Compound:1273-0060|FD-Score:3.91|P-value:2.73E-6 ID:SGTC_195|Compound:4234-0266|FD-Score:2.94|P-value:3.17E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:-2.78|P-value:5.80E-4 ID:SGTC_408|Compound:cantharidin|FD-Score:-3.06|P-value:1.80E-4 ID:SGTC_416|Compound:estradiol valerate|FD-Score:4.13|P-value:7.86E-7 ID:SGTC_549|Compound:1661-1316|FD-Score:3.34|P-value:5.38E-5 ID:SGTC_594|Compound:1487-1584|FD-Score:-2.82|P-value:4.89E-4 ID:SGTC_602|Compound:3130-1533|FD-Score:-3.10|P-value:1.50E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:4.31|P-value:2.83E-7 ID:SGTC_634|Compound:1611-4317|FD-Score:4.53|P-value:7.21E-8 ID:SGTC_638|Compound:3970-0837|FD-Score:-3.73|P-value:6.75E-6 ID:SGTC_723|Compound:4182-0001|FD-Score:-2.65|P-value:9.73E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-4.70|P-value:2.07E-8 ID:SGTC_864|Compound:0469-0573|FD-Score:3.14|P-value:1.37E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:2.93|P-value:3.44E-4 ID:SGTC_1307|Compound:1187-1599|FD-Score:-2.67|P-value:8.93E-4 ID:SGTC_1410|Compound:3909-8106|FD-Score:2.91|P-value:3.65E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:2.91|P-value:3.60E-4 ID:SGTC_1509|Compound:1326-1131|FD-Score:4.56|P-value:5.97E-8 ID:SGTC_1513|Compound:0337-0559|FD-Score:3.93|P-value:2.54E-6 ID:SGTC_1522|Compound:st012842|FD-Score:3.30|P-value:6.42E-5 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:-3.16|P-value:1.13E-4 ID:SGTC_1602|Compound:st001878|FD-Score:2.71|P-value:8.21E-4 ID:SGTC_1621|Compound:st003705|FD-Score:2.96|P-value:3.00E-4 ID:SGTC_1623|Compound:st003709|FD-Score:2.73|P-value:7.67E-4 ID:SGTC_1656|Compound:st012921|FD-Score:3.66|P-value:1.06E-5 ID:SGTC_1702|Compound:st027038|FD-Score:3.52|P-value:2.19E-5 ID:SGTC_1744|Compound:st037937|FD-Score:-3.10|P-value:1.48E-4 ID:SGTC_1749|Compound:st037932|FD-Score:3.40|P-value:3.94E-5 ID:SGTC_1893|Compound:st059353|FD-Score:3.77|P-value:5.87E-6 ID:SGTC_2031|Compound:5180455|FD-Score:-3.19|P-value:9.84E-5 ID:SGTC_2317|Compound:5986382|FD-Score:-2.71|P-value:7.87E-4 ID:SGTC_2319|Compound:6033012|FD-Score:-3.78|P-value:5.05E-6 ID:SGTC_2320|Compound:7373003|FD-Score:-2.79|P-value:5.58E-4 ID:SGTC_2377|Compound:9073351|FD-Score:2.76|P-value:6.83E-4 ID:SGTC_2384|Compound:9036245|FD-Score:2.67|P-value:9.87E-4 ID:SGTC_2393|Compound:9031399|FD-Score:3.90|P-value:2.94E-6 ID:SGTC_2394|Compound:5221648|FD-Score:-3.12|P-value:1.33E-4 ID:SGTC_2398|Compound:5228213|FD-Score:3.79|P-value:5.50E-6 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.12|P-value:1.46E-4 ID:SGTC_2456|Compound:5770359|FD-Score:2.70|P-value:8.74E-4 ID:SGTC_2457|Compound:5731572|FD-Score:2.92|P-value:3.47E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:2.68|P-value:9.33E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:-3.08|P-value:1.59E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-5.99|P-value:1.42E-12 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:3.03|P-value:2.23E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-2.95|P-value:2.86E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:4.68|P-value:2.73E-8 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.72|P-value:7.10E-6 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:3.55|P-value:1.88E-5 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:-2.76|P-value:6.26E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:2.71|P-value:8.22E-4 ID:SGTC_2805|Compound:7950783|FD-Score:-2.76|P-value:6.42E-4 ID:SGTC_2910|Compound:7948405|FD-Score:2.97|P-value:2.83E-4 ID:SGTC_3027|Compound:9089541|FD-Score:2.92|P-value:3.45E-4 ID:SGTC_3072|Compound:9116419|FD-Score:-3.98|P-value:1.69E-6 ID:SGTC_3149|Compound:9097855|FD-Score:3.21|P-value:9.72E-5 ID:SGTC_3155|Compound:9099265|FD-Score:2.91|P-value:3.61E-4 ID:SGTC_3203|Compound:9113543|FD-Score:3.91|P-value:2.73E-6 ID:SGTC_44|Compound:0929-0055|FD-Score:-2.95|P-value:2.84E-4 ID:SGTC_193|Compound:1273-0060|FD-Score:3.91|P-value:2.73E-6 ID:SGTC_195|Compound:4234-0266|FD-Score:2.94|P-value:3.17E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:-2.78|P-value:5.80E-4 ID:SGTC_408|Compound:cantharidin|FD-Score:-3.06|P-value:1.80E-4 ID:SGTC_416|Compound:estradiol valerate|FD-Score:4.13|P-value:7.86E-7 ID:SGTC_549|Compound:1661-1316|FD-Score:3.34|P-value:5.38E-5 ID:SGTC_594|Compound:1487-1584|FD-Score:-2.82|P-value:4.89E-4 ID:SGTC_602|Compound:3130-1533|FD-Score:-3.10|P-value:1.50E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:4.31|P-value:2.83E-7 ID:SGTC_634|Compound:1611-4317|FD-Score:4.53|P-value:7.21E-8 ID:SGTC_638|Compound:3970-0837|FD-Score:-3.73|P-value:6.75E-6 ID:SGTC_723|Compound:4182-0001|FD-Score:-2.65|P-value:9.73E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-4.70|P-value:2.07E-8 ID:SGTC_864|Compound:0469-0573|FD-Score:3.14|P-value:1.37E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:2.93|P-value:3.44E-4 ID:SGTC_1307|Compound:1187-1599|FD-Score:-2.67|P-value:8.93E-4 ID:SGTC_1410|Compound:3909-8106|FD-Score:2.91|P-value:3.65E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:2.91|P-value:3.60E-4 ID:SGTC_1509|Compound:1326-1131|FD-Score:4.56|P-value:5.97E-8 ID:SGTC_1513|Compound:0337-0559|FD-Score:3.93|P-value:2.54E-6 ID:SGTC_1522|Compound:st012842|FD-Score:3.30|P-value:6.42E-5 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:-3.16|P-value:1.13E-4 ID:SGTC_1602|Compound:st001878|FD-Score:2.71|P-value:8.21E-4 ID:SGTC_1621|Compound:st003705|FD-Score:2.96|P-value:3.00E-4 ID:SGTC_1623|Compound:st003709|FD-Score:2.73|P-value:7.67E-4 ID:SGTC_1656|Compound:st012921|FD-Score:3.66|P-value:1.06E-5 ID:SGTC_1702|Compound:st027038|FD-Score:3.52|P-value:2.19E-5 ID:SGTC_1744|Compound:st037937|FD-Score:-3.10|P-value:1.48E-4 ID:SGTC_1749|Compound:st037932|FD-Score:3.40|P-value:3.94E-5 ID:SGTC_1893|Compound:st059353|FD-Score:3.77|P-value:5.87E-6 ID:SGTC_2031|Compound:5180455|FD-Score:-3.19|P-value:9.84E-5 ID:SGTC_2317|Compound:5986382|FD-Score:-2.71|P-value:7.87E-4 ID:SGTC_2319|Compound:6033012|FD-Score:-3.78|P-value:5.05E-6 ID:SGTC_2320|Compound:7373003|FD-Score:-2.79|P-value:5.58E-4 ID:SGTC_2377|Compound:9073351|FD-Score:2.76|P-value:6.83E-4 ID:SGTC_2384|Compound:9036245|FD-Score:2.67|P-value:9.87E-4 ID:SGTC_2393|Compound:9031399|FD-Score:3.90|P-value:2.94E-6 ID:SGTC_2394|Compound:5221648|FD-Score:-3.12|P-value:1.33E-4 ID:SGTC_2398|Compound:5228213|FD-Score:3.79|P-value:5.50E-6 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.12|P-value:1.46E-4 ID:SGTC_2456|Compound:5770359|FD-Score:2.70|P-value:8.74E-4 ID:SGTC_2457|Compound:5731572|FD-Score:2.92|P-value:3.47E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:2.68|P-value:9.33E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:-3.08|P-value:1.59E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-5.99|P-value:1.42E-12 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:3.03|P-value:2.23E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-2.95|P-value:2.86E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:4.68|P-value:2.73E-8 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.72|P-value:7.10E-6 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:3.55|P-value:1.88E-5 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:-2.76|P-value:6.26E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:2.71|P-value:8.22E-4 ID:SGTC_2805|Compound:7950783|FD-Score:-2.76|P-value:6.42E-4 ID:SGTC_2910|Compound:7948405|FD-Score:2.97|P-value:2.83E-4 ID:SGTC_3027|Compound:9089541|FD-Score:2.92|P-value:3.45E-4 ID:SGTC_3072|Compound:9116419|FD-Score:-3.98|P-value:1.69E-6 ID:SGTC_3149|Compound:9097855|FD-Score:3.21|P-value:9.72E-5 ID:SGTC_3155|Compound:9099265|FD-Score:2.91|P-value:3.61E-4 ID:SGTC_3203|Compound:9113543|FD-Score:3.91|P-value:2.73E-6

Top fitness defect scores for YOL094C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 4.68 100.0 µM 2.73E-8 3 1.44E-6 0.10
2 SGTC_1509 1326-1131 4.56 80.0 µM 5.97E-8 3 2.59E-6 0.91
3 SGTC_634 1611-4317 4.53 11.2 µM 7.21E-8 ERG2 6 2.98E-6 0.46
4 SGTC_633 0437-0093 4.31 97.7 µM 2.83E-7 DNA intercalators 5 8.29E-6 0.06
5 SGTC_416 estradiol valerate 4.13 100.0 µM 7.86E-7 3 1.78E-5 0.24
6 SGTC_1513 0337-0559 3.93 19.0 µM 2.54E-6 7 4.29E-5 0.10
7 SGTC_193 1273-0060 3.91 1.6 µM 2.73E-6 ergosterol biosynthesis 6 4.52E-5 0.13
8 SGTC_3203 9113543 3.91 49.5 µM 2.73E-6 8 4.53E-5 0.11
9 SGTC_2393 9031399 3.90 185.9 µM 2.94E-6 RNA processing & uracil transport 11 4.79E-5 0.03
10 SGTC_2398 5228213 3.79 48.8 µM 5.50E-6 iron homeostasis 14 7.66E-5 0.04
11 SGTC_1893 st059353 3.77 36.1 µM 5.87E-6 5 8.03E-5 0.02
12 SGTC_1656 st012921 3.66 11.0 µM 1.06E-5 5 1.25E-4 0.25
13 SGTC_2634 4'-methoxyflavone 3.55 100.0 µM 1.88E-5 13 1.92E-4 0.13
14 SGTC_1702 st027038 3.52 51.0 µM 2.19E-5 5 2.16E-4 0.04
15 SGTC_1749 st037932 3.40 54.2 µM 3.94E-5 15 3.36E-4 0.03
16 SGTC_549 1661-1316 3.34 519.0 µM 5.38E-5 13 4.25E-4 0.20
17 SGTC_1522 st012842 3.30 45.6 µM 6.42E-5 RNA pol III & RNase P/MRP 21 4.85E-4 0.06
18 SGTC_3149 9097855 3.21 49.5 µM 9.72E-5 Golgi 12 6.63E-4 0.10
19 SGTC_864 0469-0573 3.14 172.0 µM 1.37E-4 10 8.58E-4 0.09
20 SGTC_2454 5-benzylidenerhodanine 3.12 10.4 µM 1.46E-4 TRP & mitochondrial translation 12 9.00E-4 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.113 4.57E-11 YNL045W LAP2 hom Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
0.108 4.18E-10 YNR003C RPC34 het RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex
0.105 1.19E-9 YBL003C HTA2 hom Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
0.098 1.10E-8 YKL090W CUE2 hom Protein of unknown function; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination
0.096 2.21E-8 YNL104C LEU4 hom Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication
0.094 5.66E-8 YJL070C_p YJL070C_p hom Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication
0.092 9.13E-8 YMR010W_p YMR010W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1
0.090 1.79E-7 YER114C BOI2 hom Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication
0.086 5.51E-7 YER127W LCP5 het Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
0.086 5.70E-7 YLR326W_p YLR326W_p hom Putative protein of unknown function, predicted to be palmitoylated
0.086 6.89E-7 YCR054C CTR86 het Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress
0.085 7.95E-7 YOR342C YOR342C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication
0.084 1.01E-6 YEL019C MMS21 het SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination
0.081 2.65E-6 YDL209C CWC2 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
0.081 2.98E-6 YNL132W KRE33 het Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance