YOL102C / TPT1

tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOL102C / TPT1

Click on Significant Values for Screen Details ID:SGTC_87|Compound:0100-0052|FD-Score:3.17|P-value:1.37E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:2.87|P-value:4.85E-4 ID:SGTC_599|Compound:1935-0052|FD-Score:3.36|P-value:5.70E-5 ID:SGTC_733|Compound:4069-0007|FD-Score:-2.74|P-value:9.14E-4 ID:SGTC_744|Compound:1594-0068|FD-Score:-6.00|P-value:3.85E-12 ID:SGTC_793|Compound:0488-0545|FD-Score:2.84|P-value:5.55E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:2.86|P-value:5.16E-4 ID:SGTC_977|Compound:1348-1378|FD-Score:3.01|P-value:2.77E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:-3.29|P-value:9.06E-5 ID:SGTC_1054|Compound:3429-0080|FD-Score:2.78|P-value:7.11E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:4.68|P-value:4.00E-8 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.85|P-value:4.96E-6 ID:SGTC_1068|Compound:loratadine|FD-Score:3.36|P-value:5.90E-5 ID:SGTC_1102|Compound:cgs 12066b|FD-Score:-2.91|P-value:4.66E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.12|P-value:1.74E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.72|P-value:9.99E-6 ID:SGTC_1567|Compound:st069303|FD-Score:2.85|P-value:5.33E-4 ID:SGTC_1626|Compound:st003712|FD-Score:-2.75|P-value:8.89E-4 ID:SGTC_1658|Compound:st012944|FD-Score:-2.77|P-value:8.10E-4 ID:SGTC_1671|Compound:st016596|FD-Score:2.97|P-value:3.27E-4 ID:SGTC_1673|Compound:st016611|FD-Score:3.57|P-value:2.14E-5 ID:SGTC_1690|Compound:st023502|FD-Score:2.90|P-value:4.28E-4 ID:SGTC_1721|Compound:st082152|FD-Score:2.71|P-value:9.26E-4 ID:SGTC_1750|Compound:st081859|FD-Score:2.71|P-value:9.42E-4 ID:SGTC_1756|Compound:st041558|FD-Score:-2.88|P-value:5.27E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.60|P-value:1.78E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:4.55|P-value:9.10E-8 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.32|P-value:6.89E-5 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:4.04|P-value:1.80E-6 ID:SGTC_2687|Compound:chenodiol|FD-Score:2.76|P-value:7.68E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-3.32|P-value:8.02E-5 ID:SGTC_2878|Compound:9043973|FD-Score:-5.04|P-value:4.61E-9 ID:SGTC_2882|Compound:9058044|FD-Score:-2.75|P-value:8.91E-4 ID:SGTC_3072|Compound:9116419|FD-Score:-3.12|P-value:1.93E-4 ID:SGTC_3275|Compound:9138069|FD-Score:-2.77|P-value:8.09E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:3.17|P-value:1.37E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:2.87|P-value:4.85E-4 ID:SGTC_599|Compound:1935-0052|FD-Score:3.36|P-value:5.70E-5 ID:SGTC_733|Compound:4069-0007|FD-Score:-2.74|P-value:9.14E-4 ID:SGTC_744|Compound:1594-0068|FD-Score:-6.00|P-value:3.85E-12 ID:SGTC_793|Compound:0488-0545|FD-Score:2.84|P-value:5.55E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:2.86|P-value:5.16E-4 ID:SGTC_977|Compound:1348-1378|FD-Score:3.01|P-value:2.77E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:-3.29|P-value:9.06E-5 ID:SGTC_1054|Compound:3429-0080|FD-Score:2.78|P-value:7.11E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:4.68|P-value:4.00E-8 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.85|P-value:4.96E-6 ID:SGTC_1068|Compound:loratadine|FD-Score:3.36|P-value:5.90E-5 ID:SGTC_1102|Compound:cgs 12066b|FD-Score:-2.91|P-value:4.66E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.12|P-value:1.74E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.72|P-value:9.99E-6 ID:SGTC_1567|Compound:st069303|FD-Score:2.85|P-value:5.33E-4 ID:SGTC_1626|Compound:st003712|FD-Score:-2.75|P-value:8.89E-4 ID:SGTC_1658|Compound:st012944|FD-Score:-2.77|P-value:8.10E-4 ID:SGTC_1671|Compound:st016596|FD-Score:2.97|P-value:3.27E-4 ID:SGTC_1673|Compound:st016611|FD-Score:3.57|P-value:2.14E-5 ID:SGTC_1690|Compound:st023502|FD-Score:2.90|P-value:4.28E-4 ID:SGTC_1721|Compound:st082152|FD-Score:2.71|P-value:9.26E-4 ID:SGTC_1750|Compound:st081859|FD-Score:2.71|P-value:9.42E-4 ID:SGTC_1756|Compound:st041558|FD-Score:-2.88|P-value:5.27E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.60|P-value:1.78E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:4.55|P-value:9.10E-8 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.32|P-value:6.89E-5 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:4.04|P-value:1.80E-6 ID:SGTC_2687|Compound:chenodiol|FD-Score:2.76|P-value:7.68E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-3.32|P-value:8.02E-5 ID:SGTC_2878|Compound:9043973|FD-Score:-5.04|P-value:4.61E-9 ID:SGTC_2882|Compound:9058044|FD-Score:-2.75|P-value:8.91E-4 ID:SGTC_3072|Compound:9116419|FD-Score:-3.12|P-value:1.93E-4 ID:SGTC_3275|Compound:9138069|FD-Score:-2.77|P-value:8.09E-4

Top fitness defect scores for YOL102C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1056 3448-1962 4.68 115.0 µM 4.00E-8 amide catabolism 3 1.42E-6 0.40
2 SGTC_1789 tunicamycin 4.55 25.0 nM 9.10E-8 unfolded protein response 10 2.67E-6 0.06
3 SGTC_2539 epi(13)torulosol 4.04 80.0 µM 1.80E-6 3 2.66E-5 0.06
4 SGTC_1061 3448-9325 3.85 110.0 µM 4.96E-6 5 5.83E-5 0.11
5 SGTC_1483 3453-0561 3.72 55.9 µM 9.99E-6 5 1.00E-4 0.35
6 SGTC_1785 bathophenanthroline 3.60 24.0 µM 1.78E-5 heme requiring 28 1.57E-4 0.02
7 SGTC_1673 st016611 3.57 27.3 µM 2.14E-5 9 1.81E-4 0.05
8 SGTC_599 1935-0052 3.36 86.5 µM 5.70E-5 7 3.86E-4 0.04
9 SGTC_1068 loratadine 3.36 26.9 µM 5.90E-5 11 3.97E-4 0.13
10 SGTC_2489 cantharidin 3.32 73.4 µM 6.89E-5 cell wall signaling 17 4.47E-4 0.01
11 SGTC_87 0100-0052 3.17 35.5 µM 1.37E-4 31 7.60E-4 0.03
12 SGTC_1150 3909-7983 3.12 10.9 µM 1.74E-4 19 9.19E-4 0.07
13 SGTC_977 1348-1378 3.01 135.0 µM 2.77E-4 18 0.00131 0.02
14 SGTC_1671 st016596 2.97 74.5 µM 3.27E-4 9 0.00150 0.11
15 SGTC_1690 st023502 2.90 41.2 µM 4.28E-4 calcium & mitochondrial duress 14 0.00185 0.02
16 SGTC_311 1315-0136 2.87 58.0 µM 4.85E-4 14 0.00203 0.08
17 SGTC_808 0711-0032 2.86 135.0 µM 5.16E-4 27 0.00213 0.03
18 SGTC_1567 st069303 2.85 68.4 µM 5.33E-4 mitochondrial processes 29 0.00219 0.03
19 SGTC_793 0488-0545 2.84 106.0 µM 5.55E-4 17 0.00226 0.01
20 SGTC_1054 3429-0080 2.78 167.0 µM 7.11E-4 20 0.00274 0.11

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.172 1.44E-23 YOR092W ECM3 hom Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication
0.171 2.68E-23 YCR007C_p YCR007C_p hom Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene
0.169 5.09E-23 YDR314C RAD34 hom Protein involved in nucleotide excision repair (NER); homologous to RAD4
0.161 8.67E-21 YNL047C SLM2 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication
0.161 7.48E-21 YOR234C RPL33B hom Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication
0.147 1.29E-17 YMR114C YMR114C hom Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene
0.142 1.08E-16 YJL010C NOP9 het Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
0.134 6.49E-15 YFL014W HSP12 hom Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.133 1.26E-14 YBL065W_d YBL065W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene
0.128 1.30E-13 YDR291W HRQ1 hom 3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS)
0.128 1.17E-13 YGR295C COS6 hom Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
0.127 2.16E-13 YDR112W_d IRC2_d hom Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci
0.118 8.26E-12 YBR243C ALG7 het UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.112 7.63E-11 YJR108W ABM1 hom Protein of unknown function, required for normal microtubule organization
0.111 1.23E-10 YOL115W PAP2 hom Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p