YOL112W / MSB4

GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL112W / MSB4

Click on Significant Values for Screen Details ID:SGTC_51|Compound:1013-0126|FD-Score:5.77|P-value:5.36E-9 ID:SGTC_54|Compound:0159-0055|FD-Score:3.15|P-value:8.92E-4 ID:SGTC_147|Compound:4466-0538|FD-Score:-4.40|P-value:6.48E-6 ID:SGTC_316|Compound:k060-0003|FD-Score:3.24|P-value:6.77E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:4.93|P-value:5.15E-7 ID:SGTC_433|Compound:pimozide|FD-Score:3.53|P-value:2.37E-4 ID:SGTC_481|Compound:bay k-8644|FD-Score:3.29|P-value:5.54E-4 ID:SGTC_616|Compound:4092-0450|FD-Score:4.82|P-value:9.07E-7 ID:SGTC_666|Compound:0272-0154|FD-Score:3.16|P-value:8.76E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:-5.27|P-value:8.92E-8 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:3.68|P-value:1.36E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:3.21|P-value:7.34E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:3.12|P-value:9.99E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:5.23|P-value:1.12E-7 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.90|P-value:5.57E-5 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.23|P-value:6.96E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:5.49|P-value:2.77E-8 ID:SGTC_1547|Compound:harmine|FD-Score:3.57|P-value:2.04E-4 ID:SGTC_1826|Compound:st055655|FD-Score:-3.97|P-value:4.17E-5 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.32|P-value:5.06E-4 ID:SGTC_1935|Compound:st074532|FD-Score:-3.33|P-value:4.89E-4 ID:SGTC_1952|Compound:st077036|FD-Score:3.82|P-value:7.84E-5 ID:SGTC_2024|Compound:5144227|FD-Score:3.87|P-value:6.27E-5 ID:SGTC_2193|Compound:5925765|FD-Score:-3.25|P-value:6.45E-4 ID:SGTC_2199|Compound:6590217|FD-Score:-3.33|P-value:4.76E-4 ID:SGTC_2226|Compound:7298446|FD-Score:-3.39|P-value:3.85E-4 ID:SGTC_2306|Compound:7482397|FD-Score:4.59|P-value:2.76E-6 ID:SGTC_2309|Compound:7664088|FD-Score:-5.32|P-value:6.71E-8 ID:SGTC_2315|Compound:7733312|FD-Score:-4.48|P-value:4.60E-6 ID:SGTC_2316|Compound:5844804|FD-Score:3.82|P-value:7.59E-5 ID:SGTC_2346|Compound:9031566|FD-Score:-3.67|P-value:1.38E-4 ID:SGTC_2348|Compound:9022543|FD-Score:3.24|P-value:6.72E-4 ID:SGTC_2390|Compound:9097792|FD-Score:3.28|P-value:5.87E-4 ID:SGTC_2457|Compound:5731572|FD-Score:3.45|P-value:3.16E-4 ID:SGTC_2470|Compound:5664817|FD-Score:3.45|P-value:3.16E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:7.46|P-value:7.40E-14 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-3.34|P-value:4.61E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:-6.07|P-value:8.87E-10 ID:SGTC_2726|Compound:cimetidine|FD-Score:4.62|P-value:2.40E-6 ID:SGTC_2759|Compound:disulfiram|FD-Score:-3.55|P-value:2.21E-4 ID:SGTC_2864|Compound:9034755|FD-Score:3.64|P-value:1.55E-4 ID:SGTC_3139|Compound:9095020|FD-Score:-3.58|P-value:1.96E-4 ID:SGTC_3222|Compound:9128301|FD-Score:3.14|P-value:9.38E-4 ID:SGTC_3265|Compound:9137266|FD-Score:-3.29|P-value:5.52E-4 ID:SGTC_3315|Compound:9136395|FD-Score:3.19|P-value:7.95E-4 ID:SGTC_3321|Compound:9137793|FD-Score:4.92|P-value:5.40E-7 ID:SGTC_51|Compound:1013-0126|FD-Score:5.77|P-value:5.36E-9 ID:SGTC_54|Compound:0159-0055|FD-Score:3.15|P-value:8.92E-4 ID:SGTC_147|Compound:4466-0538|FD-Score:-4.40|P-value:6.48E-6 ID:SGTC_316|Compound:k060-0003|FD-Score:3.24|P-value:6.77E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:4.93|P-value:5.15E-7 ID:SGTC_433|Compound:pimozide|FD-Score:3.53|P-value:2.37E-4 ID:SGTC_481|Compound:bay k-8644|FD-Score:3.29|P-value:5.54E-4 ID:SGTC_616|Compound:4092-0450|FD-Score:4.82|P-value:9.07E-7 ID:SGTC_666|Compound:0272-0154|FD-Score:3.16|P-value:8.76E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:-5.27|P-value:8.92E-8 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:3.68|P-value:1.36E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:3.21|P-value:7.34E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:3.12|P-value:9.99E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:5.23|P-value:1.12E-7 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.90|P-value:5.57E-5 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.23|P-value:6.96E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:5.49|P-value:2.77E-8 ID:SGTC_1547|Compound:harmine|FD-Score:3.57|P-value:2.04E-4 ID:SGTC_1826|Compound:st055655|FD-Score:-3.97|P-value:4.17E-5 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.32|P-value:5.06E-4 ID:SGTC_1935|Compound:st074532|FD-Score:-3.33|P-value:4.89E-4 ID:SGTC_1952|Compound:st077036|FD-Score:3.82|P-value:7.84E-5 ID:SGTC_2024|Compound:5144227|FD-Score:3.87|P-value:6.27E-5 ID:SGTC_2193|Compound:5925765|FD-Score:-3.25|P-value:6.45E-4 ID:SGTC_2199|Compound:6590217|FD-Score:-3.33|P-value:4.76E-4 ID:SGTC_2226|Compound:7298446|FD-Score:-3.39|P-value:3.85E-4 ID:SGTC_2306|Compound:7482397|FD-Score:4.59|P-value:2.76E-6 ID:SGTC_2309|Compound:7664088|FD-Score:-5.32|P-value:6.71E-8 ID:SGTC_2315|Compound:7733312|FD-Score:-4.48|P-value:4.60E-6 ID:SGTC_2316|Compound:5844804|FD-Score:3.82|P-value:7.59E-5 ID:SGTC_2346|Compound:9031566|FD-Score:-3.67|P-value:1.38E-4 ID:SGTC_2348|Compound:9022543|FD-Score:3.24|P-value:6.72E-4 ID:SGTC_2390|Compound:9097792|FD-Score:3.28|P-value:5.87E-4 ID:SGTC_2457|Compound:5731572|FD-Score:3.45|P-value:3.16E-4 ID:SGTC_2470|Compound:5664817|FD-Score:3.45|P-value:3.16E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:7.46|P-value:7.40E-14 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-3.34|P-value:4.61E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:-6.07|P-value:8.87E-10 ID:SGTC_2726|Compound:cimetidine|FD-Score:4.62|P-value:2.40E-6 ID:SGTC_2759|Compound:disulfiram|FD-Score:-3.55|P-value:2.21E-4 ID:SGTC_2864|Compound:9034755|FD-Score:3.64|P-value:1.55E-4 ID:SGTC_3139|Compound:9095020|FD-Score:-3.58|P-value:1.96E-4 ID:SGTC_3222|Compound:9128301|FD-Score:3.14|P-value:9.38E-4 ID:SGTC_3265|Compound:9137266|FD-Score:-3.29|P-value:5.52E-4 ID:SGTC_3315|Compound:9136395|FD-Score:3.19|P-value:7.95E-4 ID:SGTC_3321|Compound:9137793|FD-Score:4.92|P-value:5.40E-7

Top fitness defect scores for YOL112W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2607 solasodine 7.46 47.4 µM 7.40E-14 17 4.35E-14
2 SGTC_51 1013-0126 5.77 23.4 µM 5.36E-9 16 3.88E-9
3 SGTC_1534 6-azauridine 5.49 81.6 µM 2.77E-8 RNA pol III & RNase P/MRP 8 2.06E-8
4 SGTC_1265 0819-1001 5.23 68.8 µM 1.12E-7 6 8.57E-8
5 SGTC_362 0310-0100 4.93 9.0 µM 5.15E-7 RPP1 & pyrimidine depletion 13 4.05E-7
6 SGTC_3321 9137793 4.92 26.8 µM 5.40E-7 cell wall 45 4.25E-7
7 SGTC_616 4092-0450 4.82 108.0 µM 9.07E-7 1 7.21E-7
8 SGTC_2726 cimetidine 4.62 79.3 µM 2.40E-6 4 1.94E-6
9 SGTC_2306 7482397 4.59 179.5 µM 2.76E-6 RPP1 & pyrimidine depletion 14 2.24E-6
10 SGTC_1334 1486-0610 3.90 236.0 µM 5.57E-5 60S ribosome export 18 4.78E-5
11 SGTC_2024 5144227 3.87 134.0 µM 6.27E-5 37 5.38E-5
12 SGTC_2316 5844804 3.82 197.4 µM 7.59E-5 36 6.55E-5
13 SGTC_1952 st077036 3.82 64.1 µM 7.84E-5 39 6.76E-5
14 SGTC_1095 3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran 3.68 15.5 µM 1.36E-4 32 1.18E-4
15 SGTC_2864 9034755 3.64 47.6 µM 1.55E-4 15 1.36E-4
16 SGTC_1547 harmine 3.57 94.2 µM 2.04E-4 ergosterol biosynthesis 60 1.79E-4
17 SGTC_433 pimozide 3.53 108.0 µM 2.37E-4 27 2.08E-4
18 SGTC_2470 5664817 3.45 21.7 µM 3.16E-4 22 2.79E-4
19 SGTC_2457 5731572 3.45 11.7 µM 3.16E-4 24 2.80E-4
20 SGTC_1918 oxolan-2-ylmethyl 3-bromobenzoate 3.32 70.1 µM 5.06E-4 59 4.51E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.110 2.01E-10 YDR127W ARO1 hom Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
0.104 1.74E-9 YMR031C EIS1 hom Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress
0.103 2.25E-9 YPR097W YPR097W hom Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.102 3.13E-9 YML091C RPM2 het Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus
0.101 3.87E-9 YDL140C RPO21 het RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
0.101 4.39E-9 YDR354W TRP4 hom Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
0.099 8.17E-9 YIL166C_p YIL166C_p hom Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene
0.093 5.87E-8 YFR009W GCN20 hom Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
0.092 9.41E-8 YLR196W PWP1 het Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily
0.092 9.91E-8 YKL064W MNR2 hom Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations
0.088 2.97E-7 YPR177C_d YPR177C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex
0.087 4.49E-7 YHR080C YHR080C hom Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication
0.082 1.82E-6 YOL013W-A_p YOL013W-A_p hom Putative protein of unknown function; identified by SAGE
0.082 1.95E-6 YPR082C DIB1 het 17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein
0.079 4.27E-6 YJR046W TAH11 het DNA replication licensing factor, required for pre-replication complex assembly