YOL113W / SKM1

Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL113W / SKM1

Click on Significant Values for Screen Details ID:SGTC_42|Compound:0133-0086|FD-Score:3.54|P-value:3.47E-4 ID:SGTC_94|Compound:0334-0113|FD-Score:3.49|P-value:4.11E-4 ID:SGTC_203|Compound:k066-0004|FD-Score:4.28|P-value:2.06E-5 ID:SGTC_331|Compound:k048-0133|FD-Score:3.99|P-value:6.67E-5 ID:SGTC_518|Compound:1021-0119|FD-Score:3.85|P-value:1.15E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:4.71|P-value:3.08E-6 ID:SGTC_525|Compound:k781-2675|FD-Score:3.41|P-value:5.52E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:-4.71|P-value:2.50E-6 ID:SGTC_530|Compound:4358-1714|FD-Score:10.60|P-value:1.06E-24 ID:SGTC_533|Compound:1222-0222|FD-Score:7.05|P-value:6.26E-12 ID:SGTC_535|Compound:2293-4129|FD-Score:-3.61|P-value:2.30E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:3.38|P-value:6.00E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.98|P-value:6.94E-5 ID:SGTC_1035|Compound:k081-0041|FD-Score:4.56|P-value:6.16E-6 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.35|P-value:6.74E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:3.30|P-value:7.87E-4 ID:SGTC_1443|Compound:4112-3303|FD-Score:3.51|P-value:3.90E-4 ID:SGTC_1488|Compound:4013-0813|FD-Score:3.26|P-value:9.00E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.38|P-value:6.00E-4 ID:SGTC_1765|Compound:st044815|FD-Score:5.28|P-value:2.06E-7 ID:SGTC_1871|Compound:st057448|FD-Score:5.28|P-value:2.01E-7 ID:SGTC_2003|Compound:4023581|FD-Score:5.31|P-value:1.76E-7 ID:SGTC_2065|Compound:5221596|FD-Score:-3.34|P-value:5.79E-4 ID:SGTC_2150|Compound:5556613|FD-Score:3.35|P-value:6.79E-4 ID:SGTC_2154|Compound:5570129|FD-Score:4.15|P-value:3.42E-5 ID:SGTC_2299|Compound:7976368|FD-Score:3.45|P-value:4.79E-4 ID:SGTC_2313|Compound:7726885|FD-Score:-3.61|P-value:2.25E-4 ID:SGTC_2329|Compound:6138490|FD-Score:-3.30|P-value:6.81E-4 ID:SGTC_2415|Compound:st077333|FD-Score:6.40|P-value:4.06E-10 ID:SGTC_2442|Compound:5158906|FD-Score:-3.18|P-value:9.85E-4 ID:SGTC_2479|Compound:5617353|FD-Score:4.66|P-value:3.92E-6 ID:SGTC_2660|Compound:2',4'-dihydroxychalcone|FD-Score:3.92|P-value:8.70E-5 ID:SGTC_2765|Compound:myclobutanil|FD-Score:6.25|P-value:9.47E-10 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.64|P-value:2.44E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:4.18|P-value:3.06E-5 ID:SGTC_2849|Compound:9019321|FD-Score:7.19|P-value:2.44E-12 ID:SGTC_2858|Compound:9025781|FD-Score:3.89|P-value:9.54E-5 ID:SGTC_2936|Compound:9038235|FD-Score:3.35|P-value:6.72E-4 ID:SGTC_2962|Compound:9082311|FD-Score:3.33|P-value:7.14E-4 ID:SGTC_3020|Compound:9089140|FD-Score:-3.21|P-value:9.08E-4 ID:SGTC_3110|Compound:9122132|FD-Score:4.49|P-value:8.18E-6 ID:SGTC_3209|Compound:9113616|FD-Score:-3.43|P-value:4.33E-4 ID:SGTC_3225|Compound:9130819|FD-Score:3.40|P-value:5.69E-4 ID:SGTC_3349|Compound:9149703|FD-Score:3.38|P-value:6.03E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:3.54|P-value:3.47E-4 ID:SGTC_94|Compound:0334-0113|FD-Score:3.49|P-value:4.11E-4 ID:SGTC_203|Compound:k066-0004|FD-Score:4.28|P-value:2.06E-5 ID:SGTC_331|Compound:k048-0133|FD-Score:3.99|P-value:6.67E-5 ID:SGTC_518|Compound:1021-0119|FD-Score:3.85|P-value:1.15E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:4.71|P-value:3.08E-6 ID:SGTC_525|Compound:k781-2675|FD-Score:3.41|P-value:5.52E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:-4.71|P-value:2.50E-6 ID:SGTC_530|Compound:4358-1714|FD-Score:10.60|P-value:1.06E-24 ID:SGTC_533|Compound:1222-0222|FD-Score:7.05|P-value:6.26E-12 ID:SGTC_535|Compound:2293-4129|FD-Score:-3.61|P-value:2.30E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:3.38|P-value:6.00E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.98|P-value:6.94E-5 ID:SGTC_1035|Compound:k081-0041|FD-Score:4.56|P-value:6.16E-6 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.35|P-value:6.74E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:3.30|P-value:7.87E-4 ID:SGTC_1443|Compound:4112-3303|FD-Score:3.51|P-value:3.90E-4 ID:SGTC_1488|Compound:4013-0813|FD-Score:3.26|P-value:9.00E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.38|P-value:6.00E-4 ID:SGTC_1765|Compound:st044815|FD-Score:5.28|P-value:2.06E-7 ID:SGTC_1871|Compound:st057448|FD-Score:5.28|P-value:2.01E-7 ID:SGTC_2003|Compound:4023581|FD-Score:5.31|P-value:1.76E-7 ID:SGTC_2065|Compound:5221596|FD-Score:-3.34|P-value:5.79E-4 ID:SGTC_2150|Compound:5556613|FD-Score:3.35|P-value:6.79E-4 ID:SGTC_2154|Compound:5570129|FD-Score:4.15|P-value:3.42E-5 ID:SGTC_2299|Compound:7976368|FD-Score:3.45|P-value:4.79E-4 ID:SGTC_2313|Compound:7726885|FD-Score:-3.61|P-value:2.25E-4 ID:SGTC_2329|Compound:6138490|FD-Score:-3.30|P-value:6.81E-4 ID:SGTC_2415|Compound:st077333|FD-Score:6.40|P-value:4.06E-10 ID:SGTC_2442|Compound:5158906|FD-Score:-3.18|P-value:9.85E-4 ID:SGTC_2479|Compound:5617353|FD-Score:4.66|P-value:3.92E-6 ID:SGTC_2660|Compound:2',4'-dihydroxychalcone|FD-Score:3.92|P-value:8.70E-5 ID:SGTC_2765|Compound:myclobutanil|FD-Score:6.25|P-value:9.47E-10 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.64|P-value:2.44E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:4.18|P-value:3.06E-5 ID:SGTC_2849|Compound:9019321|FD-Score:7.19|P-value:2.44E-12 ID:SGTC_2858|Compound:9025781|FD-Score:3.89|P-value:9.54E-5 ID:SGTC_2936|Compound:9038235|FD-Score:3.35|P-value:6.72E-4 ID:SGTC_2962|Compound:9082311|FD-Score:3.33|P-value:7.14E-4 ID:SGTC_3020|Compound:9089140|FD-Score:-3.21|P-value:9.08E-4 ID:SGTC_3110|Compound:9122132|FD-Score:4.49|P-value:8.18E-6 ID:SGTC_3209|Compound:9113616|FD-Score:-3.43|P-value:4.33E-4 ID:SGTC_3225|Compound:9130819|FD-Score:3.40|P-value:5.69E-4 ID:SGTC_3349|Compound:9149703|FD-Score:3.38|P-value:6.03E-4

Top fitness defect scores for YOL113W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_530 4358-1714 10.60 28.0 µM 1.06E-24 1 1.53E-26
2 SGTC_2849 9019321 7.19 13.0 µM 2.44E-12 6 3.22E-13
3 SGTC_533 1222-0222 7.05 6.7 µM 6.26E-12 PDR1 2 8.89E-13
4 SGTC_2415 st077333 6.40 57.7 µM 4.06E-10 10 7.99E-11
5 SGTC_2765 myclobutanil 6.25 7.7 µM 9.47E-10 azole & statin 11 1.99E-10
6 SGTC_2003 4023581 5.31 200.0 µM 1.76E-7 cell wall signaling 18 5.56E-8
7 SGTC_1871 st057448 5.28 67.3 µM 2.01E-7 5 6.40E-8
8 SGTC_1765 st044815 5.28 90.4 µM 2.06E-7 7 6.59E-8
9 SGTC_519 k048-0011 4.71 62.8 µM 3.08E-6 8 1.22E-6
10 SGTC_2479 5617353 4.66 43.9 µM 3.92E-6 18 1.58E-6
11 SGTC_1035 k081-0041 4.56 55.2 µM 6.16E-6 Golgi 14 2.57E-6
12 SGTC_3110 9122132 4.49 49.5 µM 8.18E-6 redox potentiating 14 3.48E-6
13 SGTC_203 k066-0004 4.28 30.9 µM 2.06E-5 RSC & ERG11 15 9.43E-6
14 SGTC_2774 tamoxifen 4.18 10.9 µM 3.06E-5 12 1.45E-5
15 SGTC_2154 5570129 4.15 200.0 µM 3.42E-5 11 1.63E-5
16 SGTC_331 k048-0133 3.99 24.5 µM 6.67E-5 RSC & ERG11 9 3.35E-5
17 SGTC_1022 4477-0945 3.98 21.3 µM 6.94E-5 21 3.49E-5
18 SGTC_2660 2',4'-dihydroxychalcone 3.92 17.1 µM 8.70E-5 20 4.45E-5
19 SGTC_2858 9025781 3.89 9.0 µM 9.54E-5 sphingolipid biosynthesis & PDR1 103 4.92E-5
20 SGTC_518 1021-0119 3.85 62.6 µM 1.15E-4 ERG2 50 6.01E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.126 2.88E-13 YPR068C HOS1 hom Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex
0.107 4.86E-10 YOR021C SFM1 hom SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism
0.100 5.94E-9 YDR322C-A TIM11 hom Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase
0.092 1.05E-7 YOL140W ARG8 hom Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
0.089 2.27E-7 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.086 6.52E-7 YNL312W RFA2 het Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
0.084 1.17E-6 YPL202C AFT2 hom Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication
0.083 1.45E-6 YDR538W PAD1 hom Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX
0.083 1.65E-6 YBR224W_d YBR224W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1
0.080 3.55E-6 YDL174C DLD1 hom D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane
0.077 7.35E-6 YIR018W YAP5 hom Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication
0.077 8.72E-6 YGL108C YGL108C hom Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress
0.074 1.84E-5 YNL204C SPS18 hom Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation
0.074 1.95E-5 YBR207W FTH1 hom Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.073 2.07E-5 YGR189C CRH1 hom Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress