HIPHOP chemogenomics database

YOL120C / RPL18A

Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication

Zygosity: Heterozygous strain

fixedexpanded
Profile for YOL120C / RPL18A

Click on Significant Values for Screen Details

Top fitness defect scores for YOL120C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_2730	imipramine	7.80	71.3 µM	4.19E-11		1	3.02E-15	1.65
2	SGTC_959	1222-0044	7.18	107.0 µM	1.12E-9		1	3.37E-13	2.65
3	SGTC_2993	9054257	7.17	71.4 µM	1.20E-9	fatty acid desaturase (OLE1)	5	3.74E-13	0.25
4	SGTC_258	haloperidol	7.04	50.8 µM	2.35E-9	fatty acid desaturase (OLE1)	3	9.85E-13	1.33
5	SGTC_2129	girgensonine	6.97	121.0 µM	3.24E-9	heme biosynthesis & mitochondrial translocase	8	1.56E-12	0.12
6	SGTC_3007	9078383	6.86	71.4 µM	5.55E-9		1	3.37E-12	1.46
7	SGTC_1506	4226-2149	6.83	212.0 µM	6.41E-9		1	4.15E-12	2.38
8	SGTC_2725	haloperidol	6.44	53.2 µM	4.17E-8	plasma membrane duress	6	6.08E-11	0.30
9	SGTC_1761	st044296	6.37	37.0 µM	5.74E-8		1	9.61E-11	1.33
10	SGTC_800	3448-7362	6.26	155.0 µM	9.44E-8	translation	20	1.96E-10	0.02
11	SGTC_832	4083-0019	6.04	164.0 µM	2.47E-7	translation	9	7.78E-10	0.12
12	SGTC_2721	ifenprodil	6.03	61.4 µM	2.59E-7	plasma membrane duress	2	8.31E-10	1.62
13	SGTC_3081	9117243	5.86	49.5 µM	5.35E-7	fatty acid desaturase (OLE1)	13	2.35E-9	0.20
14	SGTC_1035	k081-0041	5.73	55.2 µM	9.19E-7	Golgi	3	5.09E-9	0.51
15	SGTC_941	3237-0776	5.64	460.0 µM	1.29E-6		2	8.31E-9	0.93
16	SGTC_1009	4106-0057	5.63	9.9 µM	1.40E-6		3	9.27E-9	0.37
17	SGTC_1415	3966-0071	5.49	78.1 µM	2.36E-6	DNA intercalators	1	1.96E-8	0.46
18	SGTC_2792	7535235	5.45	81.8 µM	2.77E-6	fatty acid desaturase (OLE1)	4	2.47E-8	0.77
19	SGTC_957	1187-1561	5.31	14.1 µM	4.82E-6		2	5.43E-8	0.55
20	SGTC_1465	k213-0056	5.26	144.0 µM	5.87E-6	DNA intercalators	3	7.19E-8	0.00

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.304	2.68E-72	YPL131W	RPL5	het	Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
0.251	1.99E-49	YHL015W	RPS20	het	Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
0.237	4.38E-44	YPR137W	RRP9	het	Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
0.229	6.03E-41	YLR076C_d	YLR076C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
0.227	2.22E-40	YGL055W	OLE1	het	Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
0.221	2.19E-38	YBR190W_d	YBR190W_d	het	Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
0.212	2.94E-35	YLR009W	RLP24	het	Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
0.199	3.56E-31	YGL123W	RPS2	het	Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5
0.192	3.05E-29	YOR206W	NOC2	het	Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
0.189	2.92E-28	YPR136C_d	YPR136C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
0.185	2.88E-27	YPL142C_d	YPL142C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
0.184	5.48E-27	YPL143W	RPL33A	het	Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication
0.177	3.62E-25	YOL040C	RPS15	het	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
0.174	2.53E-24	YKL180W	RPL17A	het	Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.172	1.37E-23	YGR095C	RRP46	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)