YOL133W / HRT1

RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOL133W / HRT1

Click on Significant Values for Screen Details ID:SGTC_62|Compound:0180-0423|FD-Score:3.97|P-value:3.59E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:-6.87|P-value:2.29E-12 ID:SGTC_124|Compound:0136-0217|FD-Score:4.40|P-value:5.33E-6 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.08|P-value:9.28E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:3.26|P-value:5.66E-4 ID:SGTC_228|Compound:caffeine|FD-Score:-3.27|P-value:4.83E-4 ID:SGTC_235|Compound:0330-0087|FD-Score:-3.19|P-value:6.42E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:4.22|P-value:1.21E-5 ID:SGTC_566|Compound:0958-0068|FD-Score:4.33|P-value:7.47E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:6.40|P-value:7.28E-11 ID:SGTC_619|Compound:1652-1634|FD-Score:-4.04|P-value:2.30E-5 ID:SGTC_672|Compound:1441-0018|FD-Score:3.43|P-value:3.06E-4 ID:SGTC_818|Compound:4072-2707|FD-Score:3.92|P-value:4.43E-5 ID:SGTC_858|Compound:0438-0351|FD-Score:3.72|P-value:9.99E-5 ID:SGTC_991|Compound:3996-0057|FD-Score:3.29|P-value:5.13E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.33|P-value:4.44E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:5.90|P-value:1.68E-9 ID:SGTC_1184|Compound:1269-1718|FD-Score:3.71|P-value:1.06E-4 ID:SGTC_1221|Compound:2958-7315|FD-Score:3.32|P-value:4.56E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-4.88|P-value:4.38E-7 ID:SGTC_1754|Compound:st042065|FD-Score:3.18|P-value:7.36E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.28|P-value:5.34E-4 ID:SGTC_1775|Compound:st081588|FD-Score:6.13|P-value:4.20E-10 ID:SGTC_1787|Compound:fluconazole|FD-Score:4.85|P-value:5.93E-7 ID:SGTC_1797|Compound:st048695|FD-Score:-3.09|P-value:8.88E-4 ID:SGTC_1810|Compound:st050138|FD-Score:-3.12|P-value:8.00E-4 ID:SGTC_1827|Compound:berberine|FD-Score:4.28|P-value:9.22E-6 ID:SGTC_1863|Compound:st057641|FD-Score:3.14|P-value:8.47E-4 ID:SGTC_1902|Compound:st060212|FD-Score:-3.59|P-value:1.43E-4 ID:SGTC_1922|Compound:5578187|FD-Score:-4.15|P-value:1.39E-5 ID:SGTC_1925|Compound:st057770|FD-Score:-6.79|P-value:4.00E-12 ID:SGTC_1930|Compound:st074634|FD-Score:3.79|P-value:7.42E-5 ID:SGTC_1931|Compound:st074438|FD-Score:-3.77|P-value:6.97E-5 ID:SGTC_1932|Compound:st074806|FD-Score:-4.13|P-value:1.55E-5 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.25|P-value:5.91E-4 ID:SGTC_2317|Compound:5986382|FD-Score:3.82|P-value:6.63E-5 ID:SGTC_2325|Compound:6158266|FD-Score:3.19|P-value:7.17E-4 ID:SGTC_2424|Compound:5537928|FD-Score:3.30|P-value:4.94E-4 ID:SGTC_2467|Compound:5474540|FD-Score:3.24|P-value:6.09E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.61|P-value:1.54E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.10|P-value:9.68E-4 ID:SGTC_2792|Compound:7535235|FD-Score:-3.16|P-value:6.97E-4 ID:SGTC_2794|Compound:7785382|FD-Score:5.42|P-value:2.91E-8 ID:SGTC_2843|Compound:9000894|FD-Score:3.31|P-value:4.64E-4 ID:SGTC_2844|Compound:9010103|FD-Score:4.13|P-value:1.79E-5 ID:SGTC_2941|Compound:9056145|FD-Score:3.46|P-value:2.72E-4 ID:SGTC_2948|Compound:9070189|FD-Score:3.20|P-value:7.05E-4 ID:SGTC_3089|Compound:9117425|FD-Score:3.22|P-value:6.40E-4 ID:SGTC_3093|Compound:9117344|FD-Score:3.57|P-value:1.80E-4 ID:SGTC_3209|Compound:9113616|FD-Score:-3.69|P-value:9.91E-5 ID:SGTC_3221|Compound:9128209|FD-Score:-3.07|P-value:9.67E-4 ID:SGTC_62|Compound:0180-0423|FD-Score:3.97|P-value:3.59E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:-6.87|P-value:2.29E-12 ID:SGTC_124|Compound:0136-0217|FD-Score:4.40|P-value:5.33E-6 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.08|P-value:9.28E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:3.26|P-value:5.66E-4 ID:SGTC_228|Compound:caffeine|FD-Score:-3.27|P-value:4.83E-4 ID:SGTC_235|Compound:0330-0087|FD-Score:-3.19|P-value:6.42E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:4.22|P-value:1.21E-5 ID:SGTC_566|Compound:0958-0068|FD-Score:4.33|P-value:7.47E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:6.40|P-value:7.28E-11 ID:SGTC_619|Compound:1652-1634|FD-Score:-4.04|P-value:2.30E-5 ID:SGTC_672|Compound:1441-0018|FD-Score:3.43|P-value:3.06E-4 ID:SGTC_818|Compound:4072-2707|FD-Score:3.92|P-value:4.43E-5 ID:SGTC_858|Compound:0438-0351|FD-Score:3.72|P-value:9.99E-5 ID:SGTC_991|Compound:3996-0057|FD-Score:3.29|P-value:5.13E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.33|P-value:4.44E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:5.90|P-value:1.68E-9 ID:SGTC_1184|Compound:1269-1718|FD-Score:3.71|P-value:1.06E-4 ID:SGTC_1221|Compound:2958-7315|FD-Score:3.32|P-value:4.56E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-4.88|P-value:4.38E-7 ID:SGTC_1754|Compound:st042065|FD-Score:3.18|P-value:7.36E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.28|P-value:5.34E-4 ID:SGTC_1775|Compound:st081588|FD-Score:6.13|P-value:4.20E-10 ID:SGTC_1787|Compound:fluconazole|FD-Score:4.85|P-value:5.93E-7 ID:SGTC_1797|Compound:st048695|FD-Score:-3.09|P-value:8.88E-4 ID:SGTC_1810|Compound:st050138|FD-Score:-3.12|P-value:8.00E-4 ID:SGTC_1827|Compound:berberine|FD-Score:4.28|P-value:9.22E-6 ID:SGTC_1863|Compound:st057641|FD-Score:3.14|P-value:8.47E-4 ID:SGTC_1902|Compound:st060212|FD-Score:-3.59|P-value:1.43E-4 ID:SGTC_1922|Compound:5578187|FD-Score:-4.15|P-value:1.39E-5 ID:SGTC_1925|Compound:st057770|FD-Score:-6.79|P-value:4.00E-12 ID:SGTC_1930|Compound:st074634|FD-Score:3.79|P-value:7.42E-5 ID:SGTC_1931|Compound:st074438|FD-Score:-3.77|P-value:6.97E-5 ID:SGTC_1932|Compound:st074806|FD-Score:-4.13|P-value:1.55E-5 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.25|P-value:5.91E-4 ID:SGTC_2317|Compound:5986382|FD-Score:3.82|P-value:6.63E-5 ID:SGTC_2325|Compound:6158266|FD-Score:3.19|P-value:7.17E-4 ID:SGTC_2424|Compound:5537928|FD-Score:3.30|P-value:4.94E-4 ID:SGTC_2467|Compound:5474540|FD-Score:3.24|P-value:6.09E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.61|P-value:1.54E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.10|P-value:9.68E-4 ID:SGTC_2792|Compound:7535235|FD-Score:-3.16|P-value:6.97E-4 ID:SGTC_2794|Compound:7785382|FD-Score:5.42|P-value:2.91E-8 ID:SGTC_2843|Compound:9000894|FD-Score:3.31|P-value:4.64E-4 ID:SGTC_2844|Compound:9010103|FD-Score:4.13|P-value:1.79E-5 ID:SGTC_2941|Compound:9056145|FD-Score:3.46|P-value:2.72E-4 ID:SGTC_2948|Compound:9070189|FD-Score:3.20|P-value:7.05E-4 ID:SGTC_3089|Compound:9117425|FD-Score:3.22|P-value:6.40E-4 ID:SGTC_3093|Compound:9117344|FD-Score:3.57|P-value:1.80E-4 ID:SGTC_3209|Compound:9113616|FD-Score:-3.69|P-value:9.91E-5 ID:SGTC_3221|Compound:9128209|FD-Score:-3.07|P-value:9.67E-4

Top fitness defect scores for YOL133W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_568 1193-0097 6.40 7.5 µM 7.28E-11 ERAD & cell cycle 12 7.79E-11 0.14
2 SGTC_1775 st081588 6.13 35.2 µM 4.20E-10 60S ribosome export 5 4.45E-10 0.58
3 SGTC_1089 terbinafine 5.90 2.2 µM 1.68E-9 2 1.77E-9 0.78
4 SGTC_2794 7785382 5.42 27.3 µM 2.91E-8 7 3.01E-8 0.44
5 SGTC_1787 fluconazole 4.85 80.0 µM 5.93E-7 iron homeostasis 6 6.04E-7 0.31
6 SGTC_124 0136-0217 4.40 7.1 µM 5.33E-6 mitochondrial stress 19 5.37E-6 0.08
7 SGTC_566 0958-0068 4.33 16.5 µM 7.47E-6 RSC & ERG11 16 7.51E-6 0.05
8 SGTC_1827 berberine 4.28 7.2 µM 9.22E-6 60S ribosome export 10 9.27E-6 0.31
9 SGTC_280 3448-3607 4.22 3.0 µM 1.21E-5 60S ribosome export 3 1.21E-5 0.19
10 SGTC_2844 9010103 4.13 26.0 µM 1.79E-5 8 1.80E-5 0.13
11 SGTC_62 0180-0423 3.97 3.2 µM 3.59E-5 60S ribosome export 10 3.58E-5 0.06
12 SGTC_818 4072-2707 3.92 53.5 µM 4.43E-5 9 4.42E-5 0.13
13 SGTC_2317 5986382 3.82 200.0 µM 6.63E-5 4 6.60E-5 0.14
14 SGTC_1930 st074634 3.79 34.3 µM 7.42E-5 RNA pol III & RNase P/MRP 18 7.39E-5 0.06
15 SGTC_858 0438-0351 3.72 2.8 µM 9.99E-5 copper-dependent oxidative stress 9 9.93E-5 0.16
16 SGTC_1184 1269-1718 3.71 139.0 µM 1.06E-4 7 1.05E-4 0.13
17 SGTC_2627 acetyl isogambogic acid 3.61 100.0 µM 1.54E-4 TSC3-RPN4 7 1.52E-4 0.06
18 SGTC_3093 9117344 3.57 49.5 µM 1.80E-4 60S ribosome export 9 1.78E-4 0.02
19 SGTC_2941 9056145 3.46 11.2 µM 2.72E-4 12 2.69E-4 0.25
20 SGTC_672 1441-0018 3.43 53.7 µM 3.06E-4 9 3.03E-4 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.139 6.27E-16 YOL134C_d YOL134C_d het Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit
0.137 1.32E-15 YDR054C CDC34 het Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress
0.123 7.22E-13 YKL152C GPM1 het Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis
0.106 7.51E-10 YHR151C MTC6 hom Protein of unknown function; mtc6 is synthetically sick with cdc13-1
0.105 9.38E-10 YNL163C RIA1 het Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes
0.103 2.46E-9 YLR195C NMT1 het N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
0.100 6.75E-9 YBR155W CNS1 het TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
0.098 1.49E-8 YGL217C_d YGL217C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W
0.097 1.94E-8 YDL001W RMD1 hom Cytoplasmic protein required for sporulation
0.095 4.04E-8 YER043C SAH1 het S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor
0.094 5.27E-8 YBR267W REI1 hom Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
0.092 1.09E-7 YOR232W MGE1 het Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
0.091 1.37E-7 YGR060W ERG25 het C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
0.088 2.87E-7 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.087 4.68E-7 YBR034C HMT1 hom Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region.