YOL136C / PFK27

6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL136C / PFK27

Click on Significant Values for Screen Details ID:SGTC_144|Compound:4161-2749|FD-Score:3.16|P-value:6.26E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:5.68|P-value:4.13E-9 ID:SGTC_317|Compound:4495-0002|FD-Score:3.35|P-value:3.10E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:3.07|P-value:8.64E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.59|P-value:1.29E-4 ID:SGTC_378|Compound:3970-0959|FD-Score:-5.31|P-value:6.59E-8 ID:SGTC_383|Compound:2324-0395|FD-Score:-3.22|P-value:7.31E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:4.13|P-value:1.34E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:3.30|P-value:3.79E-4 ID:SGTC_792|Compound:4534-1957|FD-Score:3.56|P-value:1.42E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:3.17|P-value:6.04E-4 ID:SGTC_860|Compound:0443-0016|FD-Score:3.77|P-value:6.21E-5 ID:SGTC_1145|Compound:3546-0597|FD-Score:4.42|P-value:3.59E-6 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.61|P-value:1.16E-4 ID:SGTC_1197|Compound:1601-0112|FD-Score:5.14|P-value:9.18E-8 ID:SGTC_1201|Compound:1889-3372|FD-Score:-3.34|P-value:4.86E-4 ID:SGTC_1439|Compound:4092-1168|FD-Score:3.06|P-value:8.76E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:3.53|P-value:1.63E-4 ID:SGTC_1670|Compound:st016595|FD-Score:4.17|P-value:1.09E-5 ID:SGTC_1685|Compound:st024049|FD-Score:3.10|P-value:7.76E-4 ID:SGTC_1743|Compound:st037606|FD-Score:3.37|P-value:2.89E-4 ID:SGTC_1962|Compound:st076595|FD-Score:5.20|P-value:6.42E-8 ID:SGTC_2005|Compound:4024620|FD-Score:3.14|P-value:6.67E-4 ID:SGTC_2287|Compound:7945887|FD-Score:3.99|P-value:2.49E-5 ID:SGTC_2338|Compound:7999974|FD-Score:3.69|P-value:8.42E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:-3.17|P-value:8.71E-4 ID:SGTC_2868|Compound:9035608|FD-Score:4.27|P-value:6.94E-6 ID:SGTC_2877|Compound:9040724|FD-Score:3.50|P-value:1.77E-4 ID:SGTC_2925|Compound:9010536|FD-Score:3.59|P-value:1.28E-4 ID:SGTC_2926|Compound:9010726|FD-Score:3.76|P-value:6.29E-5 ID:SGTC_2963|Compound:9082507|FD-Score:4.21|P-value:9.24E-6 ID:SGTC_2970|Compound:9090938|FD-Score:-3.29|P-value:5.68E-4 ID:SGTC_3185|Compound:9108267|FD-Score:3.09|P-value:8.06E-4 ID:SGTC_3218|Compound:9131285|FD-Score:-4.01|P-value:3.52E-5 ID:SGTC_144|Compound:4161-2749|FD-Score:3.16|P-value:6.26E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:5.68|P-value:4.13E-9 ID:SGTC_317|Compound:4495-0002|FD-Score:3.35|P-value:3.10E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:3.07|P-value:8.64E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.59|P-value:1.29E-4 ID:SGTC_378|Compound:3970-0959|FD-Score:-5.31|P-value:6.59E-8 ID:SGTC_383|Compound:2324-0395|FD-Score:-3.22|P-value:7.31E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:4.13|P-value:1.34E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:3.30|P-value:3.79E-4 ID:SGTC_792|Compound:4534-1957|FD-Score:3.56|P-value:1.42E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:3.17|P-value:6.04E-4 ID:SGTC_860|Compound:0443-0016|FD-Score:3.77|P-value:6.21E-5 ID:SGTC_1145|Compound:3546-0597|FD-Score:4.42|P-value:3.59E-6 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.61|P-value:1.16E-4 ID:SGTC_1197|Compound:1601-0112|FD-Score:5.14|P-value:9.18E-8 ID:SGTC_1201|Compound:1889-3372|FD-Score:-3.34|P-value:4.86E-4 ID:SGTC_1439|Compound:4092-1168|FD-Score:3.06|P-value:8.76E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:3.53|P-value:1.63E-4 ID:SGTC_1670|Compound:st016595|FD-Score:4.17|P-value:1.09E-5 ID:SGTC_1685|Compound:st024049|FD-Score:3.10|P-value:7.76E-4 ID:SGTC_1743|Compound:st037606|FD-Score:3.37|P-value:2.89E-4 ID:SGTC_1962|Compound:st076595|FD-Score:5.20|P-value:6.42E-8 ID:SGTC_2005|Compound:4024620|FD-Score:3.14|P-value:6.67E-4 ID:SGTC_2287|Compound:7945887|FD-Score:3.99|P-value:2.49E-5 ID:SGTC_2338|Compound:7999974|FD-Score:3.69|P-value:8.42E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:-3.17|P-value:8.71E-4 ID:SGTC_2868|Compound:9035608|FD-Score:4.27|P-value:6.94E-6 ID:SGTC_2877|Compound:9040724|FD-Score:3.50|P-value:1.77E-4 ID:SGTC_2925|Compound:9010536|FD-Score:3.59|P-value:1.28E-4 ID:SGTC_2926|Compound:9010726|FD-Score:3.76|P-value:6.29E-5 ID:SGTC_2963|Compound:9082507|FD-Score:4.21|P-value:9.24E-6 ID:SGTC_2970|Compound:9090938|FD-Score:-3.29|P-value:5.68E-4 ID:SGTC_3185|Compound:9108267|FD-Score:3.09|P-value:8.06E-4 ID:SGTC_3218|Compound:9131285|FD-Score:-4.01|P-value:3.52E-5

Top fitness defect scores for YOL136C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_145 3737-0113 5.68 38.4 µM 4.13E-9 DNA intercalators 32 6.56E-9
2 SGTC_1962 st076595 5.20 64.5 µM 6.42E-8 redox potentiating 12 9.71E-8
3 SGTC_1197 1601-0112 5.14 267.0 µM 9.18E-8 3 1.38E-7
4 SGTC_1145 3546-0597 4.42 10.2 µM 3.59E-6 mitochondrial processes 12 5.04E-6
5 SGTC_2868 9035608 4.27 32.5 µM 6.94E-6 12 9.62E-6
6 SGTC_2963 9082507 4.21 9.0 µM 9.24E-6 21 1.27E-5
7 SGTC_1670 st016595 4.17 4.0 µM 1.09E-5 7 1.49E-5
8 SGTC_539 4859-0462 4.13 2.5 µM 1.34E-5 RNA pol III & RNase P/MRP 21 1.83E-5
9 SGTC_2287 7945887 3.99 168.8 µM 2.49E-5 9 3.37E-5
10 SGTC_860 0443-0016 3.77 25.1 µM 6.21E-5 38 8.24E-5
11 SGTC_2926 9010726 3.76 65.2 µM 6.29E-5 37 8.34E-5
12 SGTC_2338 7999974 3.69 200.0 µM 8.42E-5 30 1.11E-4
13 SGTC_1193 1488-0126 3.61 41.5 µM 1.16E-4 35 1.52E-4
14 SGTC_2925 9010536 3.59 32.5 µM 1.28E-4 azole & statin 63 1.68E-4
15 SGTC_358 0075-0058 3.59 9.4 µM 1.29E-4 79 1.68E-4
16 SGTC_792 4534-1957 3.56 155.0 µM 1.42E-4 52 1.85E-4
17 SGTC_1588 farnesol 3.53 29.9 µM 1.63E-4 18 2.11E-4
18 SGTC_2877 9040724 3.50 71.4 µM 1.77E-4 23 2.30E-4
19 SGTC_1743 st037606 3.37 46.4 µM 2.89E-4 39 3.71E-4
20 SGTC_317 4495-0002 3.35 170.0 µM 3.10E-4 26 3.98E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.190 1.31E-28 YDR401W_d YDR401W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.161 5.15E-21 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.141 2.59E-16 YFL022C FRS2 het Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
0.135 3.60E-15 YOR077W RTS2 het Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication
0.123 7.62E-13 YNL101W AVT4 hom Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.122 1.06E-12 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.115 2.54E-11 YEL064C AVT2 hom Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.112 7.89E-11 YNL124W NAF1 het RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
0.111 1.23E-10 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.107 5.99E-10 YNL264C PDR17 hom Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition
0.104 1.29E-9 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.099 9.20E-9 YEL017C-A PMP2 hom Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
0.098 1.22E-8 YDR396W_d YDR396W_d het Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex
0.097 1.88E-8 YKR064W OAF3 hom Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress
0.096 2.69E-8 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis