YOL139C / CDC33

mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOL139C / CDC33

Click on Significant Values for Screen Details ID:SGTC_55|Compound:0767-0285|FD-Score:-4.65|P-value:3.20E-7 ID:SGTC_171|Compound:k064-0041|FD-Score:-3.08|P-value:4.79E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:2.95|P-value:8.08E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:4.12|P-value:5.26E-6 ID:SGTC_421|Compound:streptovitacin|FD-Score:4.38|P-value:1.40E-6 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:4.03|P-value:8.36E-6 ID:SGTC_431|Compound:k089-0097|FD-Score:3.08|P-value:5.02E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:3.24|P-value:2.62E-4 ID:SGTC_765|Compound:1319-0117|FD-Score:-3.34|P-value:1.75E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.65|P-value:3.36E-7 ID:SGTC_853|Compound:0419-0306|FD-Score:-2.91|P-value:9.26E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:-4.71|P-value:2.25E-7 ID:SGTC_1162|Compound:k048-0130|FD-Score:3.16|P-value:3.60E-4 ID:SGTC_1176|Compound:0848-0131|FD-Score:3.46|P-value:1.09E-4 ID:SGTC_1210|Compound:3013-0140|FD-Score:3.10|P-value:4.65E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:-3.51|P-value:8.48E-5 ID:SGTC_1266|Compound:0828-0227|FD-Score:3.32|P-value:1.94E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.74|P-value:3.20E-5 ID:SGTC_1630|Compound:st005209|FD-Score:2.93|P-value:8.73E-4 ID:SGTC_2187|Compound:6125751|FD-Score:3.40|P-value:1.36E-4 ID:SGTC_2188|Compound:6126565|FD-Score:3.13|P-value:4.04E-4 ID:SGTC_2316|Compound:5844804|FD-Score:3.51|P-value:8.71E-5 ID:SGTC_2337|Compound:9009472|FD-Score:2.97|P-value:7.60E-4 ID:SGTC_2399|Compound:5884133|FD-Score:4.57|P-value:5.21E-7 ID:SGTC_2493|Compound:resveratrol|FD-Score:2.94|P-value:8.40E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:5.63|P-value:8.59E-10 ID:SGTC_2625|Compound:euphol|FD-Score:6.87|P-value:1.00E-13 ID:SGTC_2792|Compound:7535235|FD-Score:-3.18|P-value:3.34E-4 ID:SGTC_2794|Compound:7785382|FD-Score:7.20|P-value:6.60E-15 ID:SGTC_2798|Compound:7813637|FD-Score:4.82|P-value:1.24E-7 ID:SGTC_2810|Compound:7959871|FD-Score:-3.94|P-value:1.21E-5 ID:SGTC_2812|Compound:7970281|FD-Score:3.15|P-value:3.73E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.79|P-value:1.48E-7 ID:SGTC_3021|Compound:9087658|FD-Score:4.21|P-value:3.42E-6 ID:SGTC_3024|Compound:9090631|FD-Score:4.74|P-value:1.94E-7 ID:SGTC_3042|Compound:9091578|FD-Score:3.28|P-value:2.26E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:-4.65|P-value:3.20E-7 ID:SGTC_171|Compound:k064-0041|FD-Score:-3.08|P-value:4.79E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:2.95|P-value:8.08E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:4.12|P-value:5.26E-6 ID:SGTC_421|Compound:streptovitacin|FD-Score:4.38|P-value:1.40E-6 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:4.03|P-value:8.36E-6 ID:SGTC_431|Compound:k089-0097|FD-Score:3.08|P-value:5.02E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:3.24|P-value:2.62E-4 ID:SGTC_765|Compound:1319-0117|FD-Score:-3.34|P-value:1.75E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.65|P-value:3.36E-7 ID:SGTC_853|Compound:0419-0306|FD-Score:-2.91|P-value:9.26E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:-4.71|P-value:2.25E-7 ID:SGTC_1162|Compound:k048-0130|FD-Score:3.16|P-value:3.60E-4 ID:SGTC_1176|Compound:0848-0131|FD-Score:3.46|P-value:1.09E-4 ID:SGTC_1210|Compound:3013-0140|FD-Score:3.10|P-value:4.65E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:-3.51|P-value:8.48E-5 ID:SGTC_1266|Compound:0828-0227|FD-Score:3.32|P-value:1.94E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.74|P-value:3.20E-5 ID:SGTC_1630|Compound:st005209|FD-Score:2.93|P-value:8.73E-4 ID:SGTC_2187|Compound:6125751|FD-Score:3.40|P-value:1.36E-4 ID:SGTC_2188|Compound:6126565|FD-Score:3.13|P-value:4.04E-4 ID:SGTC_2316|Compound:5844804|FD-Score:3.51|P-value:8.71E-5 ID:SGTC_2337|Compound:9009472|FD-Score:2.97|P-value:7.60E-4 ID:SGTC_2399|Compound:5884133|FD-Score:4.57|P-value:5.21E-7 ID:SGTC_2493|Compound:resveratrol|FD-Score:2.94|P-value:8.40E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:5.63|P-value:8.59E-10 ID:SGTC_2625|Compound:euphol|FD-Score:6.87|P-value:1.00E-13 ID:SGTC_2792|Compound:7535235|FD-Score:-3.18|P-value:3.34E-4 ID:SGTC_2794|Compound:7785382|FD-Score:7.20|P-value:6.60E-15 ID:SGTC_2798|Compound:7813637|FD-Score:4.82|P-value:1.24E-7 ID:SGTC_2810|Compound:7959871|FD-Score:-3.94|P-value:1.21E-5 ID:SGTC_2812|Compound:7970281|FD-Score:3.15|P-value:3.73E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.79|P-value:1.48E-7 ID:SGTC_3021|Compound:9087658|FD-Score:4.21|P-value:3.42E-6 ID:SGTC_3024|Compound:9090631|FD-Score:4.74|P-value:1.94E-7 ID:SGTC_3042|Compound:9091578|FD-Score:3.28|P-value:2.26E-4

Top fitness defect scores for YOL139C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2794 7785382 7.20 27.3 µM 6.60E-15 2 2.97E-13 1.13
2 SGTC_2625 euphol 6.87 100.0 µM 1.00E-13 1 3.23E-12 1.29
3 SGTC_2620 irigenin 5.63 100.0 µM 8.59E-10 4 8.99E-9 0.75
4 SGTC_2798 7813637 4.82 45.5 µM 1.24E-7 8 7.05E-7 0.23
5 SGTC_3024 9090631 4.74 49.5 µM 1.94E-7 1 1.04E-6 0.56
6 SGTC_800 3448-7362 4.65 155.0 µM 3.36E-7 translation 37 1.69E-6 0.09
7 SGTC_2399 5884133 4.57 200.0 µM 5.21E-7 cell wall signaling 12 2.48E-6 0.05
8 SGTC_421 streptovitacin 4.38 30.0 mM 1.40E-6 2 5.93E-6 0.73
9 SGTC_3021 9087658 4.21 49.5 µM 3.42E-6 2 1.30E-5 0.53
10 SGTC_353 1988-1175 4.12 58.3 µM 5.26E-6 unfolded protein response 8 1.89E-5 0.41
11 SGTC_423 5-fluorouracil 4.03 457.0 µM 8.36E-6 exosome 28 2.85E-5 0.07
12 SGTC_1370 2001-0092 3.74 44.6 µM 3.20E-5 5 9.27E-5 0.07
13 SGTC_2316 5844804 3.51 197.4 µM 8.71E-5 14 2.24E-4 0.00
14 SGTC_1176 0848-0131 3.46 83.3 µM 1.09E-4 Golgi 6 2.72E-4 0.08
15 SGTC_2187 6125751 3.40 200.0 µM 1.36E-4 RSC complex & mRNA processing 18 3.32E-4 0.04
16 SGTC_1266 0828-0227 3.32 32.9 µM 1.94E-4 18 4.53E-4 0.09
17 SGTC_3042 9091578 3.28 49.5 µM 2.26E-4 ERG2 2 5.18E-4 0.20
18 SGTC_610 4215-0184 3.24 155.0 µM 2.62E-4 exosome 31 5.90E-4 0.00
19 SGTC_1162 k048-0130 3.16 20.1 µM 3.60E-4 Golgi 16 7.82E-4 0.01
20 SGTC_2812 7970281 3.15 58.4 µM 3.73E-4 19 8.06E-4 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.149 4.33E-18 YGR207C CIR1 hom Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response
0.141 1.85E-16 YDL164C CDC9 het DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination
0.131 2.97E-14 YOR293C-A_p YOR293C-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.129 6.05E-14 YOR084W LPX1 hom Oleic acid-inducible, peroxisomal matrix localized lipase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; peroxisomal import is dependent on the PTS1 receptor, Pex5p and on self-interaction
0.115 2.13E-11 YFL009W CDC4 het F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p
0.115 2.64E-11 YOR371C GPB1 hom Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication
0.114 3.95E-11 YMR009W ADI1 hom Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions
0.111 1.34E-10 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.111 1.45E-10 YMR132C_p JLP2_p hom Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)
0.108 3.14E-10 YPL043W NOP4 het Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
0.108 4.19E-10 YAL056C-A_d YAL056C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.108 4.19E-10 YMR200W ROT1 het Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
0.107 4.55E-10 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.105 1.17E-9 YPL151C PRP46 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
0.102 3.75E-9 YJR016C ILV3 het Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids