YOL140W / ARG8

Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL140W / ARG8

Click on Significant Values for Screen Details ID:SGTC_54|Compound:0159-0055|FD-Score:12.00|P-value:4.77E-40 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:-2.79|P-value:9.90E-4 ID:SGTC_329|Compound:k048-0121|FD-Score:3.34|P-value:1.20E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.85|P-value:1.01E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.45|P-value:7.62E-5 ID:SGTC_530|Compound:4358-1714|FD-Score:7.59|P-value:3.15E-17 ID:SGTC_772|Compound:k072-0172|FD-Score:3.23|P-value:1.92E-4 ID:SGTC_806|Compound:3966-4455|FD-Score:-2.85|P-value:7.89E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:2.84|P-value:9.28E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:3.22|P-value:2.05E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:3.22|P-value:2.01E-4 ID:SGTC_986|Compound:1493-0483|FD-Score:3.11|P-value:3.22E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:2.92|P-value:6.75E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.09|P-value:3.51E-4 ID:SGTC_1212|Compound:0520-0165|FD-Score:3.02|P-value:4.63E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.25|P-value:1.77E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:3.33|P-value:1.28E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:5.10|P-value:1.03E-8 ID:SGTC_1412|Compound:3909-8563|FD-Score:3.00|P-value:5.05E-4 ID:SGTC_1432|Compound:4049-0298|FD-Score:2.91|P-value:6.95E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.53|P-value:4.57E-5 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:-3.23|P-value:1.66E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.36|P-value:1.12E-4 ID:SGTC_1590|Compound:st077108|FD-Score:3.29|P-value:1.54E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:-3.57|P-value:3.70E-5 ID:SGTC_1690|Compound:st023502|FD-Score:3.08|P-value:3.61E-4 ID:SGTC_1694|Compound:st024011|FD-Score:-3.22|P-value:1.77E-4 ID:SGTC_1706|Compound:st031321|FD-Score:3.68|P-value:2.59E-5 ID:SGTC_1784|Compound:st039334|FD-Score:4.31|P-value:1.06E-6 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.62|P-value:2.98E-5 ID:SGTC_2046|Compound:4110841|FD-Score:2.99|P-value:5.15E-4 ID:SGTC_2049|Compound:5262831|FD-Score:3.23|P-value:1.98E-4 ID:SGTC_2160|Compound:5660466|FD-Score:2.92|P-value:6.80E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.91|P-value:7.29E-6 ID:SGTC_2310|Compound:7668677|FD-Score:2.85|P-value:8.84E-4 ID:SGTC_2315|Compound:7733312|FD-Score:-3.45|P-value:6.43E-5 ID:SGTC_2337|Compound:9009472|FD-Score:3.19|P-value:2.34E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.02|P-value:4.06E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-4.04|P-value:3.73E-6 ID:SGTC_2581|Compound:avocatin a|FD-Score:-3.16|P-value:2.25E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:2.95|P-value:6.14E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.65|P-value:2.97E-5 ID:SGTC_2705|Compound:st078069|FD-Score:-2.89|P-value:6.74E-4 ID:SGTC_2841|Compound:9004761|FD-Score:3.28|P-value:1.59E-4 ID:SGTC_2927|Compound:9011802|FD-Score:-4.51|P-value:2.98E-7 ID:SGTC_2951|Compound:9072950|FD-Score:-2.80|P-value:9.34E-4 ID:SGTC_2972|Compound:9093619|FD-Score:2.89|P-value:7.58E-4 ID:SGTC_2986|Compound:9022445|FD-Score:2.92|P-value:6.90E-4 ID:SGTC_3066|Compound:9117245|FD-Score:3.33|P-value:1.27E-4 ID:SGTC_3077|Compound:9117319|FD-Score:-2.89|P-value:6.81E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.29|P-value:1.55E-4 ID:SGTC_3211|Compound:9128781|FD-Score:11.60|P-value:7.97E-38 ID:SGTC_54|Compound:0159-0055|FD-Score:12.00|P-value:4.77E-40 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:-2.79|P-value:9.90E-4 ID:SGTC_329|Compound:k048-0121|FD-Score:3.34|P-value:1.20E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.85|P-value:1.01E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.45|P-value:7.62E-5 ID:SGTC_530|Compound:4358-1714|FD-Score:7.59|P-value:3.15E-17 ID:SGTC_772|Compound:k072-0172|FD-Score:3.23|P-value:1.92E-4 ID:SGTC_806|Compound:3966-4455|FD-Score:-2.85|P-value:7.89E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:2.84|P-value:9.28E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:3.22|P-value:2.05E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:3.22|P-value:2.01E-4 ID:SGTC_986|Compound:1493-0483|FD-Score:3.11|P-value:3.22E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:2.92|P-value:6.75E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.09|P-value:3.51E-4 ID:SGTC_1212|Compound:0520-0165|FD-Score:3.02|P-value:4.63E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.25|P-value:1.77E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:3.33|P-value:1.28E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:5.10|P-value:1.03E-8 ID:SGTC_1412|Compound:3909-8563|FD-Score:3.00|P-value:5.05E-4 ID:SGTC_1432|Compound:4049-0298|FD-Score:2.91|P-value:6.95E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.53|P-value:4.57E-5 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:-3.23|P-value:1.66E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.36|P-value:1.12E-4 ID:SGTC_1590|Compound:st077108|FD-Score:3.29|P-value:1.54E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:-3.57|P-value:3.70E-5 ID:SGTC_1690|Compound:st023502|FD-Score:3.08|P-value:3.61E-4 ID:SGTC_1694|Compound:st024011|FD-Score:-3.22|P-value:1.77E-4 ID:SGTC_1706|Compound:st031321|FD-Score:3.68|P-value:2.59E-5 ID:SGTC_1784|Compound:st039334|FD-Score:4.31|P-value:1.06E-6 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.62|P-value:2.98E-5 ID:SGTC_2046|Compound:4110841|FD-Score:2.99|P-value:5.15E-4 ID:SGTC_2049|Compound:5262831|FD-Score:3.23|P-value:1.98E-4 ID:SGTC_2160|Compound:5660466|FD-Score:2.92|P-value:6.80E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.91|P-value:7.29E-6 ID:SGTC_2310|Compound:7668677|FD-Score:2.85|P-value:8.84E-4 ID:SGTC_2315|Compound:7733312|FD-Score:-3.45|P-value:6.43E-5 ID:SGTC_2337|Compound:9009472|FD-Score:3.19|P-value:2.34E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.02|P-value:4.06E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-4.04|P-value:3.73E-6 ID:SGTC_2581|Compound:avocatin a|FD-Score:-3.16|P-value:2.25E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:2.95|P-value:6.14E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.65|P-value:2.97E-5 ID:SGTC_2705|Compound:st078069|FD-Score:-2.89|P-value:6.74E-4 ID:SGTC_2841|Compound:9004761|FD-Score:3.28|P-value:1.59E-4 ID:SGTC_2927|Compound:9011802|FD-Score:-4.51|P-value:2.98E-7 ID:SGTC_2951|Compound:9072950|FD-Score:-2.80|P-value:9.34E-4 ID:SGTC_2972|Compound:9093619|FD-Score:2.89|P-value:7.58E-4 ID:SGTC_2986|Compound:9022445|FD-Score:2.92|P-value:6.90E-4 ID:SGTC_3066|Compound:9117245|FD-Score:3.33|P-value:1.27E-4 ID:SGTC_3077|Compound:9117319|FD-Score:-2.89|P-value:6.81E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.29|P-value:1.55E-4 ID:SGTC_3211|Compound:9128781|FD-Score:11.60|P-value:7.97E-38

Top fitness defect scores for YOL140W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_54 0159-0055 12.00 9.0 µM 4.77E-40 ERG2 7 2.52E-33
2 SGTC_3211 9128781 11.60 49.5 µM 7.97E-38 3 1.70E-31
3 SGTC_530 4358-1714 7.59 28.0 µM 3.15E-17 3 1.59E-14
4 SGTC_1263 0774-1208 5.10 64.8 µM 1.03E-8 23 1.73E-7
5 SGTC_1784 st039334 4.31 36.3 µM 1.06E-6 14 8.07E-6
6 SGTC_1706 st031321 3.68 10.0 µM 2.59E-5 TSC3-RPN4 18 1.15E-4
7 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 3.65 100.0 µM 2.97E-5 45 1.29E-4
8 SGTC_453 enantio-paf c16 3.45 10.0 µM 7.62E-5 187 2.82E-4
9 SGTC_1584 6-fluoro-dl-tryptophan 3.36 90.0 µM 1.12E-4 mitochondrial processes 36 3.89E-4
10 SGTC_329 k048-0121 3.34 21.3 µM 1.20E-4 RSC & ERG11 28 4.12E-4
11 SGTC_3066 9117245 3.33 49.5 µM 1.27E-4 23 4.33E-4
12 SGTC_1257 0669-0121 3.33 16.0 µM 1.28E-4 50 4.34E-4
13 SGTC_1590 st077108 3.29 57.8 µM 1.54E-4 18 5.07E-4
14 SGTC_3188 9110789 3.29 49.5 µM 1.55E-4 iron homeostasis 48 5.09E-4
15 SGTC_2841 9004761 3.28 39.0 µM 1.59E-4 amide catabolism 61 5.21E-4
16 SGTC_1230 0170-0365 3.25 3.8 µM 1.77E-4 endomembrane recycling 108 5.69E-4
17 SGTC_772 k072-0172 3.23 35.3 µM 1.92E-4 47 6.11E-4
18 SGTC_2049 5262831 3.23 67.2 µM 1.98E-4 64 6.26E-4
19 SGTC_968 1309-0633 3.22 340.0 µM 2.01E-4 33 6.35E-4
20 SGTC_936 3005-4620 3.22 401.0 µM 2.05E-4 56 6.45E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.415 7.66E-140 YER069W ARG5,6 hom Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
0.296 1.76E-68 YOL058W ARG1 hom Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate
0.287 1.21E-64 YJL088W ARG3 hom Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine
0.242 6.43E-46 YOR130C ORT1 hom Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome
0.236 1.64E-43 YJL071W ARG2 hom Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p
0.220 7.75E-38 YGR122C-A_d YGR122C-A_d hom Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W
0.201 1.06E-31 YER068C-A_d YER068C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.192 3.48E-29 YHR018C ARG4 hom Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway
0.180 8.56E-26 YOR303W CPA1 hom Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader
0.148 8.75E-18 YDL225W SHS1 hom Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress
0.147 1.14E-17 YDL134C PPH21 hom Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication
0.145 3.35E-17 YKL151C YKL151C hom NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
0.142 1.24E-16 YDR063W AIM7 hom Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss
0.138 8.73E-16 YJR148W BAT2 hom Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase
0.130 3.87E-14 YMR286W MRPL33 hom Mitochondrial ribosomal protein of the large subunit