AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p
Zygosity: Homozygous strain
fixedexpanded
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Top fitness defect scores for YOL141W deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.423 | 1.66E-145 | YBL036C | YBL036C | hom | Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS |
0.317 | 6.77E-79 | YLR205C | HMX1 | hom | ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants |
0.296 | 8.22E-69 | YML083C | YML083C | hom | Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions |
0.286 | 2.29E-64 | YHR003C | YHR003C | hom | Protein of unknown function, localized to the mitochondrial outer membrane |
0.267 | 1.05E-55 | YGL081W_p | YGL081W_p | hom | Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis |
0.249 | 1.30E-48 | YEL004W | YEA4 | hom | Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER |
0.239 | 6.28E-45 | YBR118W | TEF2 | hom | Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication |
0.234 | 7.79E-43 | YLL046C | RNP1 | hom | Ribonucleoprotein that contains two RNA recognition motifs (RRM) |
0.232 | 2.49E-42 | YGL146C_p | RRT6_p | hom | Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins |
0.231 | 5.70E-42 | YMR111C | YMR111C | hom | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress |
0.229 | 4.61E-41 | YDL224C | WHI4 | hom | Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication |
0.227 | 2.70E-40 | YOR345C_d | YOR345C_d | hom | Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 |
0.220 | 3.92E-38 | YGL134W | PCL10 | hom | Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate |
0.219 | 1.26E-37 | YML052W | SUR7 | hom | Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants |
0.218 | 2.13E-37 | YBL015W | ACH1 | hom | Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth |