HIPHOP chemogenomics database

YOL144W / NOP8

Nucleolar protein required for 60S ribosomal subunit biogenesis

Zygosity: Heterozygous strain

fixedexpanded
Profile for YOL144W / NOP8

Click on Significant Values for Screen Details

Top fitness defect scores for YOL144W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_568	1193-0097	14.00	7.5 µM	2.77E-29	ERAD & cell cycle	3	4.33E-45	1.92
2	SGTC_2021	4019055	13.20	173.0 µM	5.26E-26	endomembrane recycling	3	6.14E-40	0.71
3	SGTC_969	1313-0238	13.10	134.0 µM	7.61E-26	mitochondrial processes	1	1.10E-39	2.95
4	SGTC_221	trifluoperazine	13.10	10.3 µM	8.59E-26	NEO1-PIK1	5	1.33E-39	3.82
5	SGTC_2993	9054257	12.80	71.4 µM	1.08E-24	fatty acid desaturase (OLE1)	2	7.07E-38	2.24
6	SGTC_69	0096-0274	12.30	206.7 µM	4.81E-23	mitochondrial processes	2	2.76E-35	1.83
7	SGTC_730	1436-0052	12.30	41.2 µM	8.77E-23	mitochondrial processes	4	7.09E-35	6.09
8	SGTC_2183	6420625	12.00	130.5 µM	8.70E-22	mitochondrial processes	1	2.60E-33	2.17
9	SGTC_2336	9001819	11.00	198.7 µM	8.83E-19	heme biosynthesis & mitochondrial translocase	7	1.37E-28	0.70
10	SGTC_2257	7960413	10.80	200.0 µM	5.94E-18	NEO1-PIK1	3	2.72E-27	2.42
11	SGTC_1230	0170-0365	10.70	3.8 µM	8.24E-18	endomembrane recycling	6	4.55E-27	1.11
12	SGTC_1308	1187-1617	10.40	41.4 µM	7.26E-17	fatty acid desaturase (OLE1)	1	1.39E-25	3.24
13	SGTC_1803	st050068	10.20	52.4 µM	2.92E-16	NEO1-PIK1	4	1.23E-24	3.09
14	SGTC_58	0957-0393	10.10	9.0 µM	4.59E-16	cell wall	9	2.51E-24	1.45
15	SGTC_457	grayanotoxin iii	9.88	135.0 µM	2.07E-15		3	2.65E-23	2.41
16	SGTC_524	r073-0010	9.79	27.8 µM	3.48E-15		2	5.99E-23	1.00
17	SGTC_2006	4011487	9.51	200.0 µM	2.07E-14	endomembrane recycling	5	9.80E-22	1.49
18	SGTC_1754	st042065	9.33	16.1 µM	5.95E-14	NEO1-PIK1	6	5.15E-21	1.10
19	SGTC_2398	5228213	9.20	48.8 µM	1.35E-13	iron homeostasis	3	1.86E-20	1.96
20	SGTC_795	0685-0265	9.12	78.0 µM	2.12E-13	excess fatty acid	1	3.78E-20	2.56

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.558	1.64E-273	YJL086C_d	YJL086C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
0.338	4.40E-90	YCR035C	RRP43	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress
0.330	8.70E-86	YCR052W	RSC6	het	Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
0.296	1.70E-68	YNL267W	PIK1	het	Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.288	7.29E-65	YDR317W	HIM1	hom	Protein of unknown function involved in DNA repair
0.272	5.15E-58	YOR115C	TRS33	hom	One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.267	1.24E-55	YDR084C	TVP23	hom	Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.252	9.08E-50	YML037C_p	YML037C_p	hom	Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.235	4.35E-43	YMR134W	ERG29	het	Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
0.231	1.15E-41	YOR357C	SNX3	hom	Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.230	2.76E-41	YGR166W	TRS65	hom	Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.226	4.70E-40	YBR245C	ISW1	hom	ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions
0.226	6.96E-40	YCR079W	PTC6	hom	Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase
0.224	1.84E-39	YNL183C	NPR1	hom	Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.219	8.57E-38	YGR060W	ERG25	het	C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol