YOL146W / PSF3

Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOL146W / PSF3

Click on Significant Values for Screen Details ID:SGTC_41|Compound:0478-0632|FD-Score:3.22|P-value:4.83E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:3.49|P-value:1.75E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:10.80|P-value:7.95E-29 ID:SGTC_91|Compound:0417-1751|FD-Score:12.00|P-value:2.26E-35 ID:SGTC_99|Compound:0109-0120|FD-Score:-3.60|P-value:1.08E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:3.41|P-value:2.39E-4 ID:SGTC_231|Compound:b-estradiol|FD-Score:-3.12|P-value:6.86E-4 ID:SGTC_246|Compound:cycloheximide|FD-Score:-3.17|P-value:5.63E-4 ID:SGTC_389|Compound:tpck|FD-Score:8.65|P-value:3.55E-19 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.63|P-value:9.78E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.28|P-value:3.80E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.62|P-value:1.01E-4 ID:SGTC_632|Compound:0781-2353|FD-Score:4.29|P-value:5.36E-6 ID:SGTC_654|Compound:1124-0271|FD-Score:3.18|P-value:5.46E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:3.10|P-value:7.39E-4 ID:SGTC_732|Compound:3970-1727|FD-Score:3.28|P-value:3.87E-4 ID:SGTC_834|Compound:0312-0008|FD-Score:5.40|P-value:1.50E-8 ID:SGTC_878|Compound:0828-0289|FD-Score:10.80|P-value:1.26E-28 ID:SGTC_886|Compound:0866-0150|FD-Score:3.09|P-value:7.74E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:9.25|P-value:1.18E-21 ID:SGTC_1080|Compound:nisoldipine|FD-Score:3.29|P-value:3.72E-4 ID:SGTC_1081|Compound:sertraline|FD-Score:3.89|P-value:3.32E-5 ID:SGTC_1267|Compound:0828-0272|FD-Score:3.59|P-value:1.16E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:3.81|P-value:4.65E-5 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:3.28|P-value:3.85E-4 ID:SGTC_1638|Compound:st008370|FD-Score:4.49|P-value:2.08E-6 ID:SGTC_1679|Compound:4130-1276|FD-Score:4.75|P-value:5.63E-7 ID:SGTC_1735|Compound:st034310|FD-Score:3.45|P-value:2.00E-4 ID:SGTC_1748|Compound:st037455|FD-Score:-3.05|P-value:8.78E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.76|P-value:5.76E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.54|P-value:1.42E-4 ID:SGTC_1959|Compound:st077091|FD-Score:3.15|P-value:6.29E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.08|P-value:7.94E-4 ID:SGTC_2055|Compound:5235184|FD-Score:3.37|P-value:2.76E-4 ID:SGTC_2090|Compound:5301565|FD-Score:3.88|P-value:3.50E-5 ID:SGTC_2134|Compound:5324836|FD-Score:-3.18|P-value:5.51E-4 ID:SGTC_2141|Compound:5621638|FD-Score:-3.22|P-value:4.65E-4 ID:SGTC_2142|Compound:5622915|FD-Score:-3.29|P-value:3.67E-4 ID:SGTC_2144|Compound:5628481|FD-Score:-3.25|P-value:4.27E-4 ID:SGTC_2151|Compound:5563267|FD-Score:3.72|P-value:6.89E-5 ID:SGTC_2318|Compound:6029776|FD-Score:3.38|P-value:2.60E-4 ID:SGTC_2363|Compound:9053361|FD-Score:4.05|P-value:1.60E-5 ID:SGTC_2413|Compound:inermin|FD-Score:16.20|P-value:1.86E-62 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:3.15|P-value:6.18E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.15|P-value:6.08E-4 ID:SGTC_2664|Compound:lyso-paf C-16|FD-Score:3.41|P-value:2.38E-4 ID:SGTC_2676|Compound:harmalol|FD-Score:3.43|P-value:2.17E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:3.57|P-value:1.24E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:3.50|P-value:1.68E-4 ID:SGTC_2846|Compound:9008642|FD-Score:3.72|P-value:6.88E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-3.66|P-value:8.60E-5 ID:SGTC_2932|Compound:9016711|FD-Score:3.66|P-value:8.84E-5 ID:SGTC_2968|Compound:9090663|FD-Score:-3.53|P-value:1.46E-4 ID:SGTC_2982|Compound:9019404|FD-Score:3.91|P-value:2.99E-5 ID:SGTC_3030|Compound:9087619|FD-Score:3.52|P-value:1.52E-4 ID:SGTC_3037|Compound:9091026|FD-Score:-3.07|P-value:8.22E-4 ID:SGTC_3072|Compound:9116419|FD-Score:4.14|P-value:1.09E-5 ID:SGTC_3100|Compound:9120536|FD-Score:3.63|P-value:9.84E-5 ID:SGTC_3103|Compound:9120822|FD-Score:3.75|P-value:5.96E-5 ID:SGTC_3202|Compound:9113761|FD-Score:3.42|P-value:2.30E-4 ID:SGTC_3219|Compound:9131112|FD-Score:4.88|P-value:2.72E-7 ID:SGTC_41|Compound:0478-0632|FD-Score:3.22|P-value:4.83E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:3.49|P-value:1.75E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:10.80|P-value:7.95E-29 ID:SGTC_91|Compound:0417-1751|FD-Score:12.00|P-value:2.26E-35 ID:SGTC_99|Compound:0109-0120|FD-Score:-3.60|P-value:1.08E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:3.41|P-value:2.39E-4 ID:SGTC_231|Compound:b-estradiol|FD-Score:-3.12|P-value:6.86E-4 ID:SGTC_246|Compound:cycloheximide|FD-Score:-3.17|P-value:5.63E-4 ID:SGTC_389|Compound:tpck|FD-Score:8.65|P-value:3.55E-19 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.63|P-value:9.78E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.28|P-value:3.80E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.62|P-value:1.01E-4 ID:SGTC_632|Compound:0781-2353|FD-Score:4.29|P-value:5.36E-6 ID:SGTC_654|Compound:1124-0271|FD-Score:3.18|P-value:5.46E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:3.10|P-value:7.39E-4 ID:SGTC_732|Compound:3970-1727|FD-Score:3.28|P-value:3.87E-4 ID:SGTC_834|Compound:0312-0008|FD-Score:5.40|P-value:1.50E-8 ID:SGTC_878|Compound:0828-0289|FD-Score:10.80|P-value:1.26E-28 ID:SGTC_886|Compound:0866-0150|FD-Score:3.09|P-value:7.74E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:9.25|P-value:1.18E-21 ID:SGTC_1080|Compound:nisoldipine|FD-Score:3.29|P-value:3.72E-4 ID:SGTC_1081|Compound:sertraline|FD-Score:3.89|P-value:3.32E-5 ID:SGTC_1267|Compound:0828-0272|FD-Score:3.59|P-value:1.16E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:3.81|P-value:4.65E-5 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:3.28|P-value:3.85E-4 ID:SGTC_1638|Compound:st008370|FD-Score:4.49|P-value:2.08E-6 ID:SGTC_1679|Compound:4130-1276|FD-Score:4.75|P-value:5.63E-7 ID:SGTC_1735|Compound:st034310|FD-Score:3.45|P-value:2.00E-4 ID:SGTC_1748|Compound:st037455|FD-Score:-3.05|P-value:8.78E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.76|P-value:5.76E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.54|P-value:1.42E-4 ID:SGTC_1959|Compound:st077091|FD-Score:3.15|P-value:6.29E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.08|P-value:7.94E-4 ID:SGTC_2055|Compound:5235184|FD-Score:3.37|P-value:2.76E-4 ID:SGTC_2090|Compound:5301565|FD-Score:3.88|P-value:3.50E-5 ID:SGTC_2134|Compound:5324836|FD-Score:-3.18|P-value:5.51E-4 ID:SGTC_2141|Compound:5621638|FD-Score:-3.22|P-value:4.65E-4 ID:SGTC_2142|Compound:5622915|FD-Score:-3.29|P-value:3.67E-4 ID:SGTC_2144|Compound:5628481|FD-Score:-3.25|P-value:4.27E-4 ID:SGTC_2151|Compound:5563267|FD-Score:3.72|P-value:6.89E-5 ID:SGTC_2318|Compound:6029776|FD-Score:3.38|P-value:2.60E-4 ID:SGTC_2363|Compound:9053361|FD-Score:4.05|P-value:1.60E-5 ID:SGTC_2413|Compound:inermin|FD-Score:16.20|P-value:1.86E-62 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:3.15|P-value:6.18E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.15|P-value:6.08E-4 ID:SGTC_2664|Compound:lyso-paf C-16|FD-Score:3.41|P-value:2.38E-4 ID:SGTC_2676|Compound:harmalol|FD-Score:3.43|P-value:2.17E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:3.57|P-value:1.24E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:3.50|P-value:1.68E-4 ID:SGTC_2846|Compound:9008642|FD-Score:3.72|P-value:6.88E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-3.66|P-value:8.60E-5 ID:SGTC_2932|Compound:9016711|FD-Score:3.66|P-value:8.84E-5 ID:SGTC_2968|Compound:9090663|FD-Score:-3.53|P-value:1.46E-4 ID:SGTC_2982|Compound:9019404|FD-Score:3.91|P-value:2.99E-5 ID:SGTC_3030|Compound:9087619|FD-Score:3.52|P-value:1.52E-4 ID:SGTC_3037|Compound:9091026|FD-Score:-3.07|P-value:8.22E-4 ID:SGTC_3072|Compound:9116419|FD-Score:4.14|P-value:1.09E-5 ID:SGTC_3100|Compound:9120536|FD-Score:3.63|P-value:9.84E-5 ID:SGTC_3103|Compound:9120822|FD-Score:3.75|P-value:5.96E-5 ID:SGTC_3202|Compound:9113761|FD-Score:3.42|P-value:2.30E-4 ID:SGTC_3219|Compound:9131112|FD-Score:4.88|P-value:2.72E-7

Top fitness defect scores for YOL146W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2413 inermin 16.20 26.1 µM 1.86E-62 5 1.96E-59 6.57
2 SGTC_91 0417-1751 12.00 58.4 µM 2.26E-35 Golgi 1 1.05E-33 3.89
3 SGTC_81 0411-0046 10.80 10.0 µM 7.95E-29 6 1.75E-27 0.54
4 SGTC_878 0828-0289 10.80 13.7 µM 1.26E-28 4 2.71E-27 0.44
5 SGTC_964 1287-0107 9.25 19.4 µM 1.18E-21 cell wall 2 1.14E-20 1.69
6 SGTC_389 tpck 8.65 16.7 µM 3.55E-19 3 2.59E-18 3.73
7 SGTC_834 0312-0008 5.40 75.7 µM 1.50E-8 NEO1 6 3.29E-8 0.21
8 SGTC_3219 9131112 4.88 49.5 µM 2.72E-7 Golgi 11 5.17E-7 0.01
9 SGTC_1679 4130-1276 4.75 6.7 µM 5.63E-7 Golgi 18 1.03E-6 0.10
10 SGTC_1638 st008370 4.49 60.6 µM 2.08E-6 ergosterol biosynthesis 14 3.58E-6 0.18
11 SGTC_632 0781-2353 4.29 4.7 µM 5.36E-6 Golgi 15 8.83E-6 0.34
12 SGTC_3072 9116419 4.14 49.5 µM 1.09E-5 ERG2 4 1.73E-5 0.41
13 SGTC_2363 9053361 4.05 176.0 µM 1.60E-5 Golgi 11 2.51E-5 0.44
14 SGTC_2982 9019404 3.91 26.0 µM 2.99E-5 5 4.54E-5 0.59
15 SGTC_1081 sertraline 3.89 6.0 µM 3.32E-5 60S ribosome export 7 5.02E-5 0.08
16 SGTC_2090 5301565 3.88 104.5 µM 3.50E-5 TSC3-RPN4 6 5.28E-5 0.28
17 SGTC_1306 1185-0270 3.81 9.0 µM 4.65E-5 Golgi 11 6.92E-5 0.02
18 SGTC_1783 st039459 3.76 87.5 µM 5.76E-5 Golgi 10 8.49E-5 0.31
19 SGTC_3103 9120822 3.75 49.5 µM 5.96E-5 10 8.77E-5 0.30
20 SGTC_2846 9008642 3.72 19.5 µM 6.88E-5 9 1.01E-4 0.00

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.553 8.42E-269 YFL038C YPT1 het Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
0.292 3.57E-67 YDR108W TRS85 hom Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role
0.218 1.77E-37 YBR164C ARL1 hom Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor
0.203 1.05E-32 YGL113W SLD3 het Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress
0.194 1.07E-29 YBR243C ALG7 het UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.193 1.36E-29 YPL051W ARL3 hom GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor
0.191 7.10E-29 YJL004C SYS1 hom Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
0.187 8.86E-28 YOR246C ENV9 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.179 1.92E-25 YDR162C NBP2 hom Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)
0.170 4.18E-23 YGL005C COG7 hom Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.164 1.05E-21 YMR079W SEC14 het Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
0.157 7.20E-20 YML071C COG8 hom Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.147 8.79E-18 YOL149W DCP1 het Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress
0.142 1.34E-16 YML004C GLO1 hom Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress
0.141 2.25E-16 YPL082C MOT1 het Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA