YOR002W / ALG6

Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR002W / ALG6

Click on Significant Values for Screen Details ID:SGTC_18|Compound:0111-0177|FD-Score:4.25|P-value:1.57E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-8.50|P-value:7.98E-14 ID:SGTC_65|Compound:0798-0905|FD-Score:4.93|P-value:1.40E-5 ID:SGTC_72|Compound:0744-0168|FD-Score:6.33|P-value:3.35E-8 ID:SGTC_98|Compound:0074-0023|FD-Score:4.22|P-value:1.71E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-6.55|P-value:5.99E-9 ID:SGTC_115|Compound:0527-0192|FD-Score:4.66|P-value:3.72E-5 ID:SGTC_143|Compound:4032-1194|FD-Score:8.02|P-value:3.57E-12 ID:SGTC_187|Compound:1488-2104|FD-Score:4.50|P-value:6.60E-5 ID:SGTC_269|Compound:exalamide|FD-Score:4.96|P-value:1.23E-5 ID:SGTC_290|Compound:2043-6722|FD-Score:4.46|P-value:7.67E-5 ID:SGTC_304|Compound:1310-0298|FD-Score:-6.31|P-value:1.99E-8 ID:SGTC_305|Compound:1682-2595|FD-Score:3.85|P-value:5.51E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:4.10|P-value:2.51E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.70|P-value:8.75E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.89|P-value:4.85E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:4.96|P-value:1.22E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:5.48|P-value:1.52E-6 ID:SGTC_625|Compound:k213-0058|FD-Score:3.83|P-value:5.91E-4 ID:SGTC_658|Compound:3253-0951|FD-Score:4.24|P-value:1.60E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.85|P-value:5.59E-4 ID:SGTC_728|Compound:3030-5151|FD-Score:4.01|P-value:3.40E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:4.72|P-value:3.02E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:4.11|P-value:2.48E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:5.88|P-value:2.71E-7 ID:SGTC_903|Compound:1134-0042|FD-Score:5.47|P-value:1.60E-6 ID:SGTC_1078|Compound:idebenone|FD-Score:5.78|P-value:4.15E-7 ID:SGTC_1081|Compound:sertraline|FD-Score:-3.69|P-value:6.06E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:-4.00|P-value:2.29E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:3.69|P-value:9.02E-4 ID:SGTC_1250|Compound:0485-0349|FD-Score:4.63|P-value:4.23E-5 ID:SGTC_1497|Compound:4476-2978|FD-Score:3.93|P-value:4.31E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.89|P-value:2.63E-7 ID:SGTC_1776|Compound:st049249|FD-Score:4.12|P-value:2.35E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:4.45|P-value:7.95E-5 ID:SGTC_1812|Compound:st052057|FD-Score:4.18|P-value:1.95E-4 ID:SGTC_1827|Compound:berberine|FD-Score:-4.22|P-value:1.13E-4 ID:SGTC_1846|Compound:st056244|FD-Score:4.71|P-value:3.16E-5 ID:SGTC_1955|Compound:wogonin|FD-Score:-3.74|P-value:5.30E-4 ID:SGTC_2007|Compound:4011539|FD-Score:4.36|P-value:1.08E-4 ID:SGTC_2009|Compound:4012297|FD-Score:7.43|P-value:1.10E-10 ID:SGTC_2201|Compound:6605497|FD-Score:3.83|P-value:5.84E-4 ID:SGTC_2275|Compound:7947463|FD-Score:-4.03|P-value:2.09E-4 ID:SGTC_2289|Compound:7946404|FD-Score:10.80|P-value:1.68E-20 ID:SGTC_2377|Compound:9073351|FD-Score:4.68|P-value:3.46E-5 ID:SGTC_2407|Compound:paclitaxel|FD-Score:3.86|P-value:5.41E-4 ID:SGTC_2413|Compound:inermin|FD-Score:5.40|P-value:2.11E-6 ID:SGTC_2472|Compound:5377652|FD-Score:5.82|P-value:3.60E-7 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:3.82|P-value:6.05E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-3.68|P-value:6.24E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:3.79|P-value:6.68E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.76|P-value:2.59E-5 ID:SGTC_2737|Compound:bleomycin|FD-Score:5.30|P-value:3.29E-6 ID:SGTC_2795|Compound:7780010|FD-Score:4.80|P-value:2.29E-5 ID:SGTC_2896|Compound:9052656|FD-Score:5.86|P-value:3.01E-7 ID:SGTC_2994|Compound:9053520|FD-Score:4.71|P-value:3.12E-5 ID:SGTC_3032|Compound:9089427|FD-Score:-3.80|P-value:4.43E-4 ID:SGTC_3039|Compound:9090270|FD-Score:3.93|P-value:4.29E-4 ID:SGTC_3076|Compound:9116187|FD-Score:7.22|P-value:3.56E-10 ID:SGTC_3179|Compound:9107801|FD-Score:3.71|P-value:8.48E-4 ID:SGTC_3202|Compound:9113761|FD-Score:5.06|P-value:8.47E-6 ID:SGTC_3208|Compound:9114115|FD-Score:6.47|P-value:1.68E-8 ID:SGTC_3266|Compound:9136860|FD-Score:-5.34|P-value:1.63E-6 ID:SGTC_18|Compound:0111-0177|FD-Score:4.25|P-value:1.57E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-8.50|P-value:7.98E-14 ID:SGTC_65|Compound:0798-0905|FD-Score:4.93|P-value:1.40E-5 ID:SGTC_72|Compound:0744-0168|FD-Score:6.33|P-value:3.35E-8 ID:SGTC_98|Compound:0074-0023|FD-Score:4.22|P-value:1.71E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-6.55|P-value:5.99E-9 ID:SGTC_115|Compound:0527-0192|FD-Score:4.66|P-value:3.72E-5 ID:SGTC_143|Compound:4032-1194|FD-Score:8.02|P-value:3.57E-12 ID:SGTC_187|Compound:1488-2104|FD-Score:4.50|P-value:6.60E-5 ID:SGTC_269|Compound:exalamide|FD-Score:4.96|P-value:1.23E-5 ID:SGTC_290|Compound:2043-6722|FD-Score:4.46|P-value:7.67E-5 ID:SGTC_304|Compound:1310-0298|FD-Score:-6.31|P-value:1.99E-8 ID:SGTC_305|Compound:1682-2595|FD-Score:3.85|P-value:5.51E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:4.10|P-value:2.51E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.70|P-value:8.75E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.89|P-value:4.85E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:4.96|P-value:1.22E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:5.48|P-value:1.52E-6 ID:SGTC_625|Compound:k213-0058|FD-Score:3.83|P-value:5.91E-4 ID:SGTC_658|Compound:3253-0951|FD-Score:4.24|P-value:1.60E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.85|P-value:5.59E-4 ID:SGTC_728|Compound:3030-5151|FD-Score:4.01|P-value:3.40E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:4.72|P-value:3.02E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:4.11|P-value:2.48E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:5.88|P-value:2.71E-7 ID:SGTC_903|Compound:1134-0042|FD-Score:5.47|P-value:1.60E-6 ID:SGTC_1078|Compound:idebenone|FD-Score:5.78|P-value:4.15E-7 ID:SGTC_1081|Compound:sertraline|FD-Score:-3.69|P-value:6.06E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:-4.00|P-value:2.29E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:3.69|P-value:9.02E-4 ID:SGTC_1250|Compound:0485-0349|FD-Score:4.63|P-value:4.23E-5 ID:SGTC_1497|Compound:4476-2978|FD-Score:3.93|P-value:4.31E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.89|P-value:2.63E-7 ID:SGTC_1776|Compound:st049249|FD-Score:4.12|P-value:2.35E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:4.45|P-value:7.95E-5 ID:SGTC_1812|Compound:st052057|FD-Score:4.18|P-value:1.95E-4 ID:SGTC_1827|Compound:berberine|FD-Score:-4.22|P-value:1.13E-4 ID:SGTC_1846|Compound:st056244|FD-Score:4.71|P-value:3.16E-5 ID:SGTC_1955|Compound:wogonin|FD-Score:-3.74|P-value:5.30E-4 ID:SGTC_2007|Compound:4011539|FD-Score:4.36|P-value:1.08E-4 ID:SGTC_2009|Compound:4012297|FD-Score:7.43|P-value:1.10E-10 ID:SGTC_2201|Compound:6605497|FD-Score:3.83|P-value:5.84E-4 ID:SGTC_2275|Compound:7947463|FD-Score:-4.03|P-value:2.09E-4 ID:SGTC_2289|Compound:7946404|FD-Score:10.80|P-value:1.68E-20 ID:SGTC_2377|Compound:9073351|FD-Score:4.68|P-value:3.46E-5 ID:SGTC_2407|Compound:paclitaxel|FD-Score:3.86|P-value:5.41E-4 ID:SGTC_2413|Compound:inermin|FD-Score:5.40|P-value:2.11E-6 ID:SGTC_2472|Compound:5377652|FD-Score:5.82|P-value:3.60E-7 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:3.82|P-value:6.05E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-3.68|P-value:6.24E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:3.79|P-value:6.68E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.76|P-value:2.59E-5 ID:SGTC_2737|Compound:bleomycin|FD-Score:5.30|P-value:3.29E-6 ID:SGTC_2795|Compound:7780010|FD-Score:4.80|P-value:2.29E-5 ID:SGTC_2896|Compound:9052656|FD-Score:5.86|P-value:3.01E-7 ID:SGTC_2994|Compound:9053520|FD-Score:4.71|P-value:3.12E-5 ID:SGTC_3032|Compound:9089427|FD-Score:-3.80|P-value:4.43E-4 ID:SGTC_3039|Compound:9090270|FD-Score:3.93|P-value:4.29E-4 ID:SGTC_3076|Compound:9116187|FD-Score:7.22|P-value:3.56E-10 ID:SGTC_3179|Compound:9107801|FD-Score:3.71|P-value:8.48E-4 ID:SGTC_3202|Compound:9113761|FD-Score:5.06|P-value:8.47E-6 ID:SGTC_3208|Compound:9114115|FD-Score:6.47|P-value:1.68E-8 ID:SGTC_3266|Compound:9136860|FD-Score:-5.34|P-value:1.63E-6

Top fitness defect scores for YOR002W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2289 7946404 10.80 165.1 µM 1.68E-20 7 2.91E-27
2 SGTC_143 4032-1194 8.02 15.7 µM 3.57E-12 9 5.23E-16
3 SGTC_2009 4012297 7.43 12.1 µM 1.10E-10 cell wall signaling 16 5.40E-14
4 SGTC_3076 9116187 7.22 49.5 µM 3.56E-10 2 2.63E-13
5 SGTC_3208 9114115 6.47 49.5 µM 1.68E-8 8 4.80E-11
6 SGTC_72 0744-0168 6.33 198.4 µM 3.35E-8 RPP1 & pyrimidine depletion 6 1.21E-10
7 SGTC_1597 7-benzyloxygramine 5.89 47.5 µM 2.63E-7 calcium & mitochondrial duress 53 1.96E-9
8 SGTC_830 4076-0286 5.88 57.8 µM 2.71E-7 translation 18 2.04E-9
9 SGTC_2896 9052656 5.86 71.4 µM 3.01E-7 endomembrane recycling 41 2.36E-9
10 SGTC_2472 5377652 5.82 49.4 µM 3.60E-7 TRP & mitochondrial translation 22 3.00E-9
11 SGTC_1078 idebenone 5.78 36.1 µM 4.15E-7 Golgi 14 3.63E-9
12 SGTC_528 3492-0019 5.48 172.0 µM 1.52E-6 calcium & mitochondrial duress 140 2.10E-8
13 SGTC_903 1134-0042 5.47 114.0 µM 1.60E-6 7 2.25E-8
14 SGTC_2413 inermin 5.40 26.1 µM 2.11E-6 25 3.25E-8
15 SGTC_2737 bleomycin 5.30 30.5 nM 3.29E-6 Golgi 40 5.93E-8
16 SGTC_3202 9113761 5.06 49.5 µM 8.47E-6 21 2.12E-7
17 SGTC_498 2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ) 4.96 150.0 µM 1.22E-5 Golgi 27 3.47E-7
18 SGTC_269 exalamide 4.96 29.0 µM 1.23E-5 ergosterol biosynthesis 7 3.50E-7
19 SGTC_65 0798-0905 4.93 35.0 µM 1.40E-5 calcium & mitochondrial duress 54 4.17E-7
20 SGTC_2795 7780010 4.80 54.5 µM 2.29E-5 11 8.13E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.299 2.80E-70 YOR067C ALG8 hom Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p
0.290 7.43E-66 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.262 8.54E-54 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.224 2.02E-39 YKL096C-B_p YKL096C-B_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.223 6.25E-39 YBL082C ALG3 hom Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.208 4.32E-34 YGR166W TRS65 hom Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.206 1.61E-33 YML115C VAN1 hom Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant
0.201 4.67E-32 YNL322C KRE1 hom Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.201 5.28E-32 YNL219C ALG9 hom Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
0.201 9.05E-32 YAL058W CNE1 hom Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
0.194 7.77E-30 YJR075W HOC1 hom Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele
0.174 2.75E-24 YBR229C ROT2 hom Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
0.171 1.59E-23 YBL083C_d YBL083C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3
0.171 2.43E-23 YAL053W FLC2 hom Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
0.167 1.85E-22 YDL095W PMT1 hom Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen