YOR005C / DNL4

DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR005C / DNL4

Click on Significant Values for Screen Details ID:SGTC_35|Compound:0133-0054|FD-Score:-3.43|P-value:1.61E-4 ID:SGTC_233|Compound:rapamycin|FD-Score:-3.19|P-value:4.22E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:3.20|P-value:3.34E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:3.42|P-value:1.38E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:2.99|P-value:7.28E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:6.04|P-value:7.56E-11 ID:SGTC_632|Compound:0781-2353|FD-Score:3.41|P-value:1.43E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:3.61|P-value:6.39E-5 ID:SGTC_809|Compound:3998-0021|FD-Score:3.35|P-value:1.87E-4 ID:SGTC_814|Compound:3342-0569|FD-Score:-3.44|P-value:1.55E-4 ID:SGTC_818|Compound:4072-2707|FD-Score:-4.76|P-value:2.97E-7 ID:SGTC_919|Compound:tnp00227|FD-Score:3.47|P-value:1.13E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:3.04|P-value:6.06E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.44|P-value:1.28E-4 ID:SGTC_1176|Compound:0848-0131|FD-Score:3.84|P-value:2.24E-5 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.44|P-value:1.27E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:-3.08|P-value:6.32E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:3.04|P-value:6.12E-4 ID:SGTC_1531|Compound:st059297|FD-Score:4.20|P-value:4.20E-6 ID:SGTC_1534|Compound:6-azauridine|FD-Score:2.93|P-value:8.94E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:3.35|P-value:1.82E-4 ID:SGTC_1686|Compound:st019653|FD-Score:2.91|P-value:9.81E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:3.24|P-value:2.87E-4 ID:SGTC_1729|Compound:st035615|FD-Score:3.42|P-value:1.37E-4 ID:SGTC_1866|Compound:st057638|FD-Score:2.92|P-value:9.59E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.40|P-value:1.50E-4 ID:SGTC_1987|Compound:st072290|FD-Score:3.18|P-value:3.62E-4 ID:SGTC_2430|Compound:5571206|FD-Score:2.97|P-value:8.01E-4 ID:SGTC_2431|Compound:5490079|FD-Score:3.33|P-value:2.03E-4 ID:SGTC_2685|Compound:mycophenolic acid|FD-Score:3.28|P-value:2.44E-4 ID:SGTC_2806|Compound:7952793|FD-Score:7.73|P-value:1.44E-16 ID:SGTC_2807|Compound:7945238|FD-Score:2.96|P-value:8.22E-4 ID:SGTC_2810|Compound:7959871|FD-Score:7.75|P-value:1.13E-16 ID:SGTC_2813|Compound:7971799|FD-Score:3.92|P-value:1.56E-5 ID:SGTC_3205|Compound:9113483|FD-Score:4.45|P-value:1.15E-6 ID:SGTC_3218|Compound:9131285|FD-Score:3.33|P-value:1.97E-4 ID:SGTC_3230|Compound:9131665|FD-Score:3.04|P-value:6.17E-4 ID:SGTC_3252|Compound:9135871|FD-Score:3.05|P-value:5.95E-4 ID:SGTC_3258|Compound:9138477|FD-Score:-3.10|P-value:5.91E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:-3.43|P-value:1.61E-4 ID:SGTC_233|Compound:rapamycin|FD-Score:-3.19|P-value:4.22E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:3.20|P-value:3.34E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:3.42|P-value:1.38E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:2.99|P-value:7.28E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:6.04|P-value:7.56E-11 ID:SGTC_632|Compound:0781-2353|FD-Score:3.41|P-value:1.43E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:3.61|P-value:6.39E-5 ID:SGTC_809|Compound:3998-0021|FD-Score:3.35|P-value:1.87E-4 ID:SGTC_814|Compound:3342-0569|FD-Score:-3.44|P-value:1.55E-4 ID:SGTC_818|Compound:4072-2707|FD-Score:-4.76|P-value:2.97E-7 ID:SGTC_919|Compound:tnp00227|FD-Score:3.47|P-value:1.13E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:3.04|P-value:6.06E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.44|P-value:1.28E-4 ID:SGTC_1176|Compound:0848-0131|FD-Score:3.84|P-value:2.24E-5 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.44|P-value:1.27E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:-3.08|P-value:6.32E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:3.04|P-value:6.12E-4 ID:SGTC_1531|Compound:st059297|FD-Score:4.20|P-value:4.20E-6 ID:SGTC_1534|Compound:6-azauridine|FD-Score:2.93|P-value:8.94E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:3.35|P-value:1.82E-4 ID:SGTC_1686|Compound:st019653|FD-Score:2.91|P-value:9.81E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:3.24|P-value:2.87E-4 ID:SGTC_1729|Compound:st035615|FD-Score:3.42|P-value:1.37E-4 ID:SGTC_1866|Compound:st057638|FD-Score:2.92|P-value:9.59E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.40|P-value:1.50E-4 ID:SGTC_1987|Compound:st072290|FD-Score:3.18|P-value:3.62E-4 ID:SGTC_2430|Compound:5571206|FD-Score:2.97|P-value:8.01E-4 ID:SGTC_2431|Compound:5490079|FD-Score:3.33|P-value:2.03E-4 ID:SGTC_2685|Compound:mycophenolic acid|FD-Score:3.28|P-value:2.44E-4 ID:SGTC_2806|Compound:7952793|FD-Score:7.73|P-value:1.44E-16 ID:SGTC_2807|Compound:7945238|FD-Score:2.96|P-value:8.22E-4 ID:SGTC_2810|Compound:7959871|FD-Score:7.75|P-value:1.13E-16 ID:SGTC_2813|Compound:7971799|FD-Score:3.92|P-value:1.56E-5 ID:SGTC_3205|Compound:9113483|FD-Score:4.45|P-value:1.15E-6 ID:SGTC_3218|Compound:9131285|FD-Score:3.33|P-value:1.97E-4 ID:SGTC_3230|Compound:9131665|FD-Score:3.04|P-value:6.17E-4 ID:SGTC_3252|Compound:9135871|FD-Score:3.05|P-value:5.95E-4 ID:SGTC_3258|Compound:9138477|FD-Score:-3.10|P-value:5.91E-4

Top fitness defect scores for YOR005C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2810 7959871 7.75 45.5 µM 1.13E-16 copper-dependent oxidative stress 6 4.49E-15
2 SGTC_2806 7952793 7.73 58.4 µM 1.44E-16 3 5.56E-15
3 SGTC_623 k072-0108 6.04 16.9 µM 7.56E-11 heme biosynthesis & mitochondrial translocase 1 7.50E-10
4 SGTC_3205 9113483 4.45 49.5 µM 1.15E-6 17 4.23E-6
5 SGTC_1531 st059297 4.20 52.4 µM 4.20E-6 11 1.35E-5
6 SGTC_2813 7971799 3.92 71.4 µM 1.56E-5 21 4.39E-5
7 SGTC_1176 0848-0131 3.84 83.3 µM 2.24E-5 Golgi 25 6.09E-5
8 SGTC_803 4488-1585 3.61 147.0 µM 6.39E-5 20 1.56E-4
9 SGTC_919 tnp00227 3.47 403.6 nM 1.13E-4 RNA pol III & RNase P/MRP 60 2.60E-4
10 SGTC_1368 1852-0141 3.44 284.0 µM 1.27E-4 ERG2 56 2.89E-4
11 SGTC_1004 4084-0029 3.44 199.0 µM 1.28E-4 33 2.90E-4
12 SGTC_1729 st035615 3.42 46.0 µM 1.37E-4 53 3.10E-4
13 SGTC_576 1073-0113 3.42 22.7 µM 1.38E-4 redox potentiating 84 3.12E-4
14 SGTC_632 0781-2353 3.41 4.7 µM 1.43E-4 Golgi 66 3.22E-4
15 SGTC_1907 st060210 3.40 83.7 µM 1.50E-4 41 3.35E-4
16 SGTC_1535 tetrahydropapaveroline hydrobromide 3.35 54.3 µM 1.82E-4 RPP1 & pyrimidine depletion 25 3.98E-4
17 SGTC_809 3998-0021 3.35 90.0 µM 1.87E-4 RNA processing & uracil transport 21 4.10E-4
18 SGTC_3218 9131285 3.33 49.5 µM 1.97E-4 endomembrane recycling 81 4.28E-4
19 SGTC_2431 5490079 3.33 200.0 µM 2.03E-4 62 4.40E-4
20 SGTC_2685 mycophenolic acid 3.28 18.9 µM 2.44E-4 RNA pol III & RNase P/MRP 20 5.20E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.135 5.00E-15 YNL142W MEP2 hom Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation
0.131 2.55E-14 YLR191W PEX13 hom Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p
0.127 1.64E-13 YPR112C MRD1 het Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region
0.106 8.31E-10 YER154W OXA1 hom Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals
0.105 9.72E-10 YJR124C_p YJR124C_p hom Putative protein of unknown function; expression induced under calcium shortage
0.103 2.43E-9 YER148W SPT15 het TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
0.101 4.36E-9 YJL016W_p YJL016W_p hom Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species
0.099 7.69E-9 YBL009W ALK2 hom Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins
0.096 2.60E-8 YFR014C CMK1 hom Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication
0.095 3.46E-8 YPL182C_d YPL182C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W
0.095 3.54E-8 YDR513W GRX2 hom Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication
0.094 4.98E-8 YNL261W ORC5 het Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
0.092 8.95E-8 YDR036C EHD3 hom 3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis
0.089 2.45E-7 YDR498C SEC20 het Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p
0.087 4.06E-7 YER001W MNN1 hom Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family