YOR018W / ROD1

Membrane protein, binds the ubiquitin ligase Rsp5p via its 2 PY motifs; overexpression confers resistance to the GST substrate o-dinitrobenzene, zinc, and calcium; proposed to regulate the endocytosis of plasma membrane proteins; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR018W / ROD1

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:4.53|P-value:3.74E-7 ID:SGTC_65|Compound:0798-0905|FD-Score:3.46|P-value:7.94E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:3.37|P-value:1.20E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.23|P-value:1.95E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:3.27|P-value:1.86E-4 ID:SGTC_334|Compound:1502-1024|FD-Score:2.85|P-value:9.51E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.79|P-value:1.82E-5 ID:SGTC_450|Compound:aminopyrene|FD-Score:3.86|P-value:1.30E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.51|P-value:4.75E-13 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:4.90|P-value:4.40E-8 ID:SGTC_484|Compound:loperamide|FD-Score:3.60|P-value:4.31E-5 ID:SGTC_530|Compound:4358-1714|FD-Score:3.61|P-value:4.11E-5 ID:SGTC_575|Compound:k072-0246|FD-Score:2.98|P-value:5.92E-4 ID:SGTC_718|Compound:1130-0073|FD-Score:3.00|P-value:5.48E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:3.12|P-value:3.43E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:3.92|P-value:9.54E-6 ID:SGTC_910|Compound:3454-3003|FD-Score:4.65|P-value:1.94E-7 ID:SGTC_932|Compound:2857-0803|FD-Score:3.00|P-value:5.39E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:2.98|P-value:5.91E-4 ID:SGTC_1009|Compound:4106-0057|FD-Score:3.38|P-value:1.14E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:-4.50|P-value:3.82E-7 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.67|P-value:3.07E-5 ID:SGTC_1152|Compound:3909-8331|FD-Score:3.58|P-value:4.70E-5 ID:SGTC_1274|Compound:0865-0114|FD-Score:4.49|P-value:4.72E-7 ID:SGTC_1277|Compound:0873-0036|FD-Score:3.34|P-value:1.38E-4 ID:SGTC_1430|Compound:4049-0248|FD-Score:2.86|P-value:9.17E-4 ID:SGTC_1441|Compound:4106-0040|FD-Score:4.79|P-value:8.51E-8 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.17|P-value:2.42E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:4.19|P-value:2.38E-6 ID:SGTC_1616|Compound:st002734|FD-Score:4.24|P-value:1.82E-6 ID:SGTC_1633|Compound:st005664|FD-Score:-2.96|P-value:5.70E-4 ID:SGTC_1799|Compound:st045822|FD-Score:3.03|P-value:4.85E-4 ID:SGTC_1826|Compound:st055655|FD-Score:3.30|P-value:1.61E-4 ID:SGTC_1836|Compound:st055642|FD-Score:2.98|P-value:5.78E-4 ID:SGTC_1867|Compound:st057372|FD-Score:-3.08|P-value:3.47E-4 ID:SGTC_1878|Compound:temozolomide|FD-Score:3.98|P-value:6.95E-6 ID:SGTC_1880|Compound:MG-132|FD-Score:3.47|P-value:7.61E-5 ID:SGTC_2070|Compound:5230947|FD-Score:-3.46|P-value:7.10E-5 ID:SGTC_2101|Compound:5377231|FD-Score:3.01|P-value:5.30E-4 ID:SGTC_2305|Compound:7443522|FD-Score:3.44|P-value:8.79E-5 ID:SGTC_2306|Compound:7482397|FD-Score:4.76|P-value:1.04E-7 ID:SGTC_2315|Compound:7733312|FD-Score:-2.97|P-value:5.34E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-2.94|P-value:5.98E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:5.57|P-value:5.86E-10 ID:SGTC_2611|Compound:pyromycin|FD-Score:-3.78|P-value:1.65E-5 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:-2.98|P-value:5.22E-4 ID:SGTC_2845|Compound:9003024|FD-Score:-3.37|P-value:1.04E-4 ID:SGTC_2975|Compound:9084847|FD-Score:2.94|P-value:6.80E-4 ID:SGTC_3030|Compound:9087619|FD-Score:3.20|P-value:2.43E-4 ID:SGTC_3137|Compound:9097352|FD-Score:3.72|P-value:2.46E-5 ID:SGTC_3190|Compound:9110366|FD-Score:3.03|P-value:4.75E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:4.53|P-value:3.74E-7 ID:SGTC_65|Compound:0798-0905|FD-Score:3.46|P-value:7.94E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:3.37|P-value:1.20E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.23|P-value:1.95E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:3.27|P-value:1.86E-4 ID:SGTC_334|Compound:1502-1024|FD-Score:2.85|P-value:9.51E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.79|P-value:1.82E-5 ID:SGTC_450|Compound:aminopyrene|FD-Score:3.86|P-value:1.30E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.51|P-value:4.75E-13 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:4.90|P-value:4.40E-8 ID:SGTC_484|Compound:loperamide|FD-Score:3.60|P-value:4.31E-5 ID:SGTC_530|Compound:4358-1714|FD-Score:3.61|P-value:4.11E-5 ID:SGTC_575|Compound:k072-0246|FD-Score:2.98|P-value:5.92E-4 ID:SGTC_718|Compound:1130-0073|FD-Score:3.00|P-value:5.48E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:3.12|P-value:3.43E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:3.92|P-value:9.54E-6 ID:SGTC_910|Compound:3454-3003|FD-Score:4.65|P-value:1.94E-7 ID:SGTC_932|Compound:2857-0803|FD-Score:3.00|P-value:5.39E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:2.98|P-value:5.91E-4 ID:SGTC_1009|Compound:4106-0057|FD-Score:3.38|P-value:1.14E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:-4.50|P-value:3.82E-7 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.67|P-value:3.07E-5 ID:SGTC_1152|Compound:3909-8331|FD-Score:3.58|P-value:4.70E-5 ID:SGTC_1274|Compound:0865-0114|FD-Score:4.49|P-value:4.72E-7 ID:SGTC_1277|Compound:0873-0036|FD-Score:3.34|P-value:1.38E-4 ID:SGTC_1430|Compound:4049-0248|FD-Score:2.86|P-value:9.17E-4 ID:SGTC_1441|Compound:4106-0040|FD-Score:4.79|P-value:8.51E-8 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.17|P-value:2.42E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:4.19|P-value:2.38E-6 ID:SGTC_1616|Compound:st002734|FD-Score:4.24|P-value:1.82E-6 ID:SGTC_1633|Compound:st005664|FD-Score:-2.96|P-value:5.70E-4 ID:SGTC_1799|Compound:st045822|FD-Score:3.03|P-value:4.85E-4 ID:SGTC_1826|Compound:st055655|FD-Score:3.30|P-value:1.61E-4 ID:SGTC_1836|Compound:st055642|FD-Score:2.98|P-value:5.78E-4 ID:SGTC_1867|Compound:st057372|FD-Score:-3.08|P-value:3.47E-4 ID:SGTC_1878|Compound:temozolomide|FD-Score:3.98|P-value:6.95E-6 ID:SGTC_1880|Compound:MG-132|FD-Score:3.47|P-value:7.61E-5 ID:SGTC_2070|Compound:5230947|FD-Score:-3.46|P-value:7.10E-5 ID:SGTC_2101|Compound:5377231|FD-Score:3.01|P-value:5.30E-4 ID:SGTC_2305|Compound:7443522|FD-Score:3.44|P-value:8.79E-5 ID:SGTC_2306|Compound:7482397|FD-Score:4.76|P-value:1.04E-7 ID:SGTC_2315|Compound:7733312|FD-Score:-2.97|P-value:5.34E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-2.94|P-value:5.98E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:5.57|P-value:5.86E-10 ID:SGTC_2611|Compound:pyromycin|FD-Score:-3.78|P-value:1.65E-5 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:-2.98|P-value:5.22E-4 ID:SGTC_2845|Compound:9003024|FD-Score:-3.37|P-value:1.04E-4 ID:SGTC_2975|Compound:9084847|FD-Score:2.94|P-value:6.80E-4 ID:SGTC_3030|Compound:9087619|FD-Score:3.20|P-value:2.43E-4 ID:SGTC_3137|Compound:9097352|FD-Score:3.72|P-value:2.46E-5 ID:SGTC_3190|Compound:9110366|FD-Score:3.03|P-value:4.75E-4

Top fitness defect scores for YOR018W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2604 4'-hydroxychalcone 5.57 11.3 µM 5.86E-10 8 1.26E-8
2 SGTC_479 n-linoleoylglycine 4.90 10.0 µM 4.40E-8 4 4.75E-7
3 SGTC_1441 4106-0040 4.79 13.0 µM 8.51E-8 2 8.28E-7
4 SGTC_2306 7482397 4.76 179.5 µM 1.04E-7 RPP1 & pyrimidine depletion 11 9.80E-7
5 SGTC_910 3454-3003 4.65 490.0 µM 1.94E-7 7 1.66E-6
6 SGTC_64 000l-0018 4.53 272.1 µM 3.74E-7 ubiquinone biosynthesis & proteasome 121 2.88E-6
7 SGTC_1274 0865-0114 4.49 173.0 µM 4.72E-7 13 3.51E-6
8 SGTC_1616 st002734 4.24 56.1 µM 1.82E-6 23 1.09E-5
9 SGTC_1535 tetrahydropapaveroline hydrobromide 4.19 54.3 µM 2.38E-6 RPP1 & pyrimidine depletion 6 1.37E-5
10 SGTC_1878 temozolomide 3.98 1.0 mM 6.95E-6 6 3.39E-5
11 SGTC_798 1287-0182 3.92 476.0 µM 9.54E-6 23 4.42E-5
12 SGTC_450 aminopyrene 3.86 156.0 nM 1.30E-5 34 5.75E-5
13 SGTC_423 5-fluorouracil 3.79 457.0 µM 1.82E-5 exosome 28 7.63E-5
14 SGTC_3137 9097352 3.72 49.5 µM 2.46E-5 49 9.86E-5
15 SGTC_1083 didemethylchlorpromazine sulfoxide 3.67 707.4 nM 3.07E-5 redox potentiating 96 1.19E-4
16 SGTC_530 4358-1714 3.61 28.0 µM 4.11E-5 54 1.52E-4
17 SGTC_484 loperamide 3.60 105.0 µM 4.31E-5 5 1.58E-4
18 SGTC_1152 3909-8331 3.58 15.9 µM 4.70E-5 19 1.70E-4
19 SGTC_1880 MG-132 3.47 525.0 µM 7.61E-5 31 2.56E-4
20 SGTC_65 0798-0905 3.46 35.0 µM 7.94E-5 calcium & mitochondrial duress 118 2.65E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.166 3.52E-22 YDR148C KGD2 hom Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated
0.155 1.41E-19 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.150 2.37E-18 YJR091C JSN1 hom Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl
0.133 1.16E-14 YOR125C CAT5 hom Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
0.125 3.95E-13 YDR204W COQ4 hom Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.124 6.46E-13 YFL056C AAD6 hom Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin
0.123 7.02E-13 YLR199C PBA1 hom Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly
0.121 2.37E-12 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.119 4.78E-12 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.118 6.73E-12 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.116 1.61E-11 YDR512C EMI1 hom Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs
0.116 1.69E-11 YMR037C MSN2 hom Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.115 2.77E-11 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.114 3.46E-11 YDR431W_d YDR431W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.111 1.27E-10 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p