YOR026W / BUB3

Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR026W / BUB3

Click on Significant Values for Screen Details ID:SGTC_51|Compound:1013-0126|FD-Score:-3.10|P-value:9.03E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.90|P-value:4.25E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:4.21|P-value:1.09E-5 ID:SGTC_163|Compound:1327-0036|FD-Score:6.08|P-value:4.31E-10 ID:SGTC_164|Compound:4100-3994|FD-Score:-3.11|P-value:8.59E-4 ID:SGTC_170|Compound:4197-0004|FD-Score:-3.73|P-value:8.60E-5 ID:SGTC_193|Compound:1273-0060|FD-Score:-3.64|P-value:1.22E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:3.10|P-value:8.74E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.20|P-value:6.17E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.39|P-value:4.85E-6 ID:SGTC_354|Compound:2783-3500|FD-Score:4.13|P-value:1.54E-5 ID:SGTC_360|Compound:0957-0399|FD-Score:4.20|P-value:1.17E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.23|P-value:5.67E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:-3.12|P-value:8.42E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:3.09|P-value:9.23E-4 ID:SGTC_814|Compound:3342-0569|FD-Score:3.52|P-value:1.96E-4 ID:SGTC_864|Compound:0469-0573|FD-Score:-3.75|P-value:7.77E-5 ID:SGTC_1014|Compound:4130-3005|FD-Score:3.38|P-value:3.22E-4 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.38|P-value:3.27E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:4.44|P-value:3.80E-6 ID:SGTC_1178|Compound:0988-0033|FD-Score:4.02|P-value:2.57E-5 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.35|P-value:3.60E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.45|P-value:2.57E-4 ID:SGTC_1367|Compound:1812-0510|FD-Score:3.76|P-value:7.63E-5 ID:SGTC_1568|Compound:digitonin|FD-Score:3.10|P-value:8.85E-4 ID:SGTC_1622|Compound:st003707|FD-Score:3.71|P-value:9.18E-5 ID:SGTC_2054|Compound:5233173|FD-Score:4.72|P-value:9.95E-7 ID:SGTC_2105|Compound:5350118|FD-Score:3.11|P-value:8.47E-4 ID:SGTC_2113|Compound:5491891|FD-Score:3.09|P-value:9.18E-4 ID:SGTC_2137|Compound:5261020|FD-Score:4.64|P-value:1.43E-6 ID:SGTC_2190|Compound:6049624|FD-Score:3.70|P-value:9.52E-5 ID:SGTC_2194|Compound:5927483|FD-Score:-3.49|P-value:2.20E-4 ID:SGTC_2210|Compound:7115885|FD-Score:3.90|P-value:4.25E-5 ID:SGTC_2211|Compound:7117706|FD-Score:-3.36|P-value:3.52E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:3.91|P-value:3.99E-5 ID:SGTC_2413|Compound:inermin|FD-Score:3.25|P-value:5.19E-4 ID:SGTC_2474|Compound:5321570|FD-Score:3.19|P-value:6.47E-4 ID:SGTC_2477|Compound:5967686|FD-Score:3.33|P-value:3.92E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:4.87|P-value:4.47E-7 ID:SGTC_2995|Compound:9067767|FD-Score:5.12|P-value:1.23E-7 ID:SGTC_3009|Compound:9080819|FD-Score:3.27|P-value:4.90E-4 ID:SGTC_3166|Compound:9102297|FD-Score:3.82|P-value:5.81E-5 ID:SGTC_3278|Compound:9139605|FD-Score:3.73|P-value:8.40E-5 ID:SGTC_3286|Compound:9117135|FD-Score:3.37|P-value:3.43E-4 ID:SGTC_51|Compound:1013-0126|FD-Score:-3.10|P-value:9.03E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.90|P-value:4.25E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:4.21|P-value:1.09E-5 ID:SGTC_163|Compound:1327-0036|FD-Score:6.08|P-value:4.31E-10 ID:SGTC_164|Compound:4100-3994|FD-Score:-3.11|P-value:8.59E-4 ID:SGTC_170|Compound:4197-0004|FD-Score:-3.73|P-value:8.60E-5 ID:SGTC_193|Compound:1273-0060|FD-Score:-3.64|P-value:1.22E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:3.10|P-value:8.74E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.20|P-value:6.17E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.39|P-value:4.85E-6 ID:SGTC_354|Compound:2783-3500|FD-Score:4.13|P-value:1.54E-5 ID:SGTC_360|Compound:0957-0399|FD-Score:4.20|P-value:1.17E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.23|P-value:5.67E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:-3.12|P-value:8.42E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:3.09|P-value:9.23E-4 ID:SGTC_814|Compound:3342-0569|FD-Score:3.52|P-value:1.96E-4 ID:SGTC_864|Compound:0469-0573|FD-Score:-3.75|P-value:7.77E-5 ID:SGTC_1014|Compound:4130-3005|FD-Score:3.38|P-value:3.22E-4 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.38|P-value:3.27E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:4.44|P-value:3.80E-6 ID:SGTC_1178|Compound:0988-0033|FD-Score:4.02|P-value:2.57E-5 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.35|P-value:3.60E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.45|P-value:2.57E-4 ID:SGTC_1367|Compound:1812-0510|FD-Score:3.76|P-value:7.63E-5 ID:SGTC_1568|Compound:digitonin|FD-Score:3.10|P-value:8.85E-4 ID:SGTC_1622|Compound:st003707|FD-Score:3.71|P-value:9.18E-5 ID:SGTC_2054|Compound:5233173|FD-Score:4.72|P-value:9.95E-7 ID:SGTC_2105|Compound:5350118|FD-Score:3.11|P-value:8.47E-4 ID:SGTC_2113|Compound:5491891|FD-Score:3.09|P-value:9.18E-4 ID:SGTC_2137|Compound:5261020|FD-Score:4.64|P-value:1.43E-6 ID:SGTC_2190|Compound:6049624|FD-Score:3.70|P-value:9.52E-5 ID:SGTC_2194|Compound:5927483|FD-Score:-3.49|P-value:2.20E-4 ID:SGTC_2210|Compound:7115885|FD-Score:3.90|P-value:4.25E-5 ID:SGTC_2211|Compound:7117706|FD-Score:-3.36|P-value:3.52E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:3.91|P-value:3.99E-5 ID:SGTC_2413|Compound:inermin|FD-Score:3.25|P-value:5.19E-4 ID:SGTC_2474|Compound:5321570|FD-Score:3.19|P-value:6.47E-4 ID:SGTC_2477|Compound:5967686|FD-Score:3.33|P-value:3.92E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:4.87|P-value:4.47E-7 ID:SGTC_2995|Compound:9067767|FD-Score:5.12|P-value:1.23E-7 ID:SGTC_3009|Compound:9080819|FD-Score:3.27|P-value:4.90E-4 ID:SGTC_3166|Compound:9102297|FD-Score:3.82|P-value:5.81E-5 ID:SGTC_3278|Compound:9139605|FD-Score:3.73|P-value:8.40E-5 ID:SGTC_3286|Compound:9117135|FD-Score:3.37|P-value:3.43E-4

Top fitness defect scores for YOR026W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_163 1327-0036 6.08 7.5 µM 4.31E-10 45 5.89E-10
2 SGTC_2995 9067767 5.12 71.4 µM 1.23E-7 9 1.53E-7
3 SGTC_2596 sinensetin 4.87 100.0 µM 4.47E-7 14 5.48E-7
4 SGTC_2054 5233173 4.72 129.0 µM 9.95E-7 mitochondrial response to ROS 34 1.20E-6
5 SGTC_2137 5261020 4.64 140.0 µM 1.43E-6 25 1.72E-6
6 SGTC_1110 3-hydroxy-3'-methoxyflavone 4.44 1.2 µM 3.80E-6 iron homeostasis 33 4.50E-6
7 SGTC_351 caspofungin 4.39 25.0 nM 4.85E-6 cell wall 80 5.73E-6
8 SGTC_138 0850-0259 4.21 40.9 µM 1.09E-5 cell wall 182 1.27E-5
9 SGTC_360 0957-0399 4.20 1.0 µM 1.17E-5 cell wall 38 1.36E-5
10 SGTC_354 2783-3500 4.13 4.9 µM 1.54E-5 cell wall 44 1.79E-5
11 SGTC_1178 0988-0033 4.02 191.0 µM 2.57E-5 24 2.95E-5
12 SGTC_2409 alverine citrate 3.91 64.0 µM 3.99E-5 53 4.56E-5
13 SGTC_2210 7115885 3.90 190.4 µM 4.25E-5 cell wall signaling 34 4.85E-5
14 SGTC_58 0957-0393 3.90 9.0 µM 4.25E-5 cell wall 376 4.86E-5
15 SGTC_3166 9102297 3.82 49.5 µM 5.81E-5 Golgi 34 6.60E-5
16 SGTC_1367 1812-0510 3.76 76.9 µM 7.63E-5 27 8.64E-5
17 SGTC_3278 9139605 3.73 49.5 µM 8.40E-5 20 9.50E-5
18 SGTC_1622 st003707 3.71 19.4 µM 9.18E-5 calcium & mitochondrial duress 49 1.04E-4
19 SGTC_2190 6049624 3.70 200.0 µM 9.52E-5 6 1.07E-4
20 SGTC_814 3342-0569 3.52 96.0 µM 1.96E-4 30 2.19E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.207 9.33E-34 YDR351W SBE2 hom Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
0.198 4.78E-31 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.187 1.02E-27 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.177 4.42E-25 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.172 1.44E-23 YPL066W RGL1 hom Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
0.164 1.49E-21 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.164 1.57E-21 YLL021W SPA2 hom Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate
0.163 2.00E-21 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.163 2.13E-21 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.156 1.23E-19 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.154 2.47E-19 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.147 9.92E-18 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.143 9.80E-17 YDR515W SLF1 hom RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.137 1.65E-15 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.135 3.45E-15 YDL189W RBS1 hom Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain