YOR054C / VHS3

Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR054C / VHS3

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:4.76|P-value:2.17E-6 ID:SGTC_74|Compound:000s-0487|FD-Score:-4.54|P-value:5.35E-6 ID:SGTC_336|Compound:4111-0029|FD-Score:3.46|P-value:4.25E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:-3.56|P-value:2.75E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:3.60|P-value:2.57E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-6.08|P-value:1.84E-9 ID:SGTC_996|Compound:3970-0799|FD-Score:-5.05|P-value:4.74E-7 ID:SGTC_998|Compound:3996-0085|FD-Score:-6.40|P-value:2.76E-10 ID:SGTC_1109|Compound:tnp00067|FD-Score:3.46|P-value:4.16E-4 ID:SGTC_1460|Compound:k062-0059|FD-Score:3.99|P-value:5.91E-5 ID:SGTC_1464|Compound:k208-0027|FD-Score:4.51|P-value:6.47E-6 ID:SGTC_1874|Compound:staurosporine|FD-Score:3.53|P-value:3.22E-4 ID:SGTC_1963|Compound:st076565|FD-Score:3.53|P-value:3.28E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.56|P-value:2.99E-4 ID:SGTC_2328|Compound:6201408|FD-Score:-3.31|P-value:6.57E-4 ID:SGTC_2348|Compound:9022543|FD-Score:3.41|P-value:5.05E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-3.58|P-value:2.53E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:5.29|P-value:1.60E-7 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-3.66|P-value:1.89E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:3.25|P-value:8.57E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:3.21|P-value:9.83E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:-3.29|P-value:7.05E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-5.48|P-value:5.13E-8 ID:SGTC_64|Compound:000l-0018|FD-Score:4.76|P-value:2.17E-6 ID:SGTC_74|Compound:000s-0487|FD-Score:-4.54|P-value:5.35E-6 ID:SGTC_336|Compound:4111-0029|FD-Score:3.46|P-value:4.25E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:-3.56|P-value:2.75E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:3.60|P-value:2.57E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-6.08|P-value:1.84E-9 ID:SGTC_996|Compound:3970-0799|FD-Score:-5.05|P-value:4.74E-7 ID:SGTC_998|Compound:3996-0085|FD-Score:-6.40|P-value:2.76E-10 ID:SGTC_1109|Compound:tnp00067|FD-Score:3.46|P-value:4.16E-4 ID:SGTC_1460|Compound:k062-0059|FD-Score:3.99|P-value:5.91E-5 ID:SGTC_1464|Compound:k208-0027|FD-Score:4.51|P-value:6.47E-6 ID:SGTC_1874|Compound:staurosporine|FD-Score:3.53|P-value:3.22E-4 ID:SGTC_1963|Compound:st076565|FD-Score:3.53|P-value:3.28E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.56|P-value:2.99E-4 ID:SGTC_2328|Compound:6201408|FD-Score:-3.31|P-value:6.57E-4 ID:SGTC_2348|Compound:9022543|FD-Score:3.41|P-value:5.05E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-3.58|P-value:2.53E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:5.29|P-value:1.60E-7 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-3.66|P-value:1.89E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:3.25|P-value:8.57E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:3.21|P-value:9.83E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:-3.29|P-value:7.05E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-5.48|P-value:5.13E-8

Top fitness defect scores for YOR054C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2538 3',7'-epoxycaryophyllan-6-one 5.29 100.0 µM 1.60E-7 7 6.17E-8
2 SGTC_64 000l-0018 4.76 272.1 µM 2.17E-6 ubiquinone biosynthesis & proteasome 116 9.92E-7
3 SGTC_1464 k208-0027 4.51 110.0 µM 6.47E-6 15 3.18E-6
4 SGTC_1460 k062-0059 3.99 9.2 µM 5.91E-5 Golgi 13 3.35E-5
5 SGTC_430 0958-0115 3.60 16.4 µM 2.57E-4 38 1.60E-4
6 SGTC_2066 5222451 3.56 176.0 µM 2.99E-4 heme biosynthesis & mitochondrial translocase 47 1.89E-4
7 SGTC_1874 staurosporine 3.53 7.6 µM 3.22E-4 cell wall signaling 40 2.05E-4
8 SGTC_1963 st076565 3.53 50.3 µM 3.28E-4 22 2.08E-4
9 SGTC_1109 tnp00067 3.46 928.3 nM 4.16E-4 29 2.68E-4
10 SGTC_336 4111-0029 3.46 111.0 µM 4.25E-4 14 2.74E-4
11 SGTC_2348 9022543 3.41 33.4 µM 5.05E-4 mitochondrial processes 48 3.30E-4
12 SGTC_2614 arthonioic acid 3.25 13.4 µM 8.57E-4 46 5.79E-4
13 SGTC_2759 disulfiram 3.21 3.4 µM 9.83E-4 69 6.70E-4
14 SGTC_1427 4048-4900 3.20 550.0 nM 0.00101 55 6.87E-4
15 SGTC_207 4185-0010 3.16 4.5 µM 0.00114 endomembrane recycling 135 7.88E-4
16 SGTC_383 2324-0395 3.04 7.4 µM 0.00168 azole & statin 89 0.00118
17 SGTC_1015 4130-4525 3.01 82.0 µM 0.00184 60S ribosome export 64 0.00131
18 SGTC_1455 4262-0120 2.98 131.0 µM 0.00202 plasma membrane duress 75 0.00145
19 SGTC_3196 9111891 2.96 49.5 µM 0.00213 mitochondrial processes 46 0.00152
20 SGTC_203 k066-0004 2.91 30.9 µM 0.00251 RSC & ERG11 73 0.00182

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.148 7.62E-18 YDL140C RPO21 het RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
0.131 2.19E-14 YLR021W IRC25 hom Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.121 1.81E-12 YLR014C PPR1 hom Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p
0.118 8.21E-12 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.117 1.19E-11 YKL085W MDH1 hom Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated
0.116 1.87E-11 YMR314W PRE5 het Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.107 4.29E-10 YDL235C YPD1 het Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus
0.106 7.26E-10 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.105 1.26E-9 YBL013W FMT1 hom Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate
0.103 1.90E-9 YER094C PUP3 het Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
0.102 3.60E-9 YLR199C PBA1 hom Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly
0.096 2.58E-8 YML014W TRM9 hom tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
0.094 5.07E-8 YOR064C YNG1 hom Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1
0.093 6.38E-8 YMR268C PRP24 het Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
0.093 7.38E-8 YBR128C ATG14 hom Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor