YOR067C / ALG8

Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR067C / ALG8

Click on Significant Values for Screen Details ID:SGTC_56|Compound:0957-0365|FD-Score:6.22|P-value:1.40E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:-6.50|P-value:3.88E-8 ID:SGTC_61|Compound:0978-8327|FD-Score:5.18|P-value:9.43E-6 ID:SGTC_103|Compound:0844-0104|FD-Score:-3.92|P-value:5.92E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:5.07|P-value:1.39E-5 ID:SGTC_187|Compound:1488-2104|FD-Score:3.96|P-value:5.28E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.39|P-value:1.41E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:4.22|P-value:2.47E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.92|P-value:5.96E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:9.01|P-value:4.71E-14 ID:SGTC_514|Compound:4466-0038|FD-Score:-4.05|P-value:4.08E-4 ID:SGTC_518|Compound:1021-0119|FD-Score:6.09|P-value:2.46E-7 ID:SGTC_528|Compound:3492-0019|FD-Score:9.33|P-value:6.21E-15 ID:SGTC_542|Compound:3381-0244|FD-Score:5.09|P-value:1.31E-5 ID:SGTC_562|Compound:3772-2733|FD-Score:4.45|P-value:1.19E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:5.32|P-value:5.51E-6 ID:SGTC_658|Compound:3253-0951|FD-Score:6.32|P-value:8.92E-8 ID:SGTC_708|Compound:3448-5400|FD-Score:4.26|P-value:2.14E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:4.28|P-value:2.04E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.71|P-value:1.20E-6 ID:SGTC_830|Compound:4076-0286|FD-Score:6.83|P-value:8.43E-9 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:4.51|P-value:9.80E-5 ID:SGTC_1055|Compound:3448-0672|FD-Score:5.74|P-value:1.03E-6 ID:SGTC_1090|Compound:efavirenz|FD-Score:4.56|P-value:8.30E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:-5.58|P-value:2.01E-6 ID:SGTC_1189|Compound:1431-2119|FD-Score:6.30|P-value:9.62E-8 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.85|P-value:7.43E-4 ID:SGTC_1206|Compound:3978-0017|FD-Score:7.65|P-value:1.31E-10 ID:SGTC_1212|Compound:0520-0165|FD-Score:9.76|P-value:3.74E-16 ID:SGTC_1252|Compound:0493-0875|FD-Score:4.35|P-value:1.62E-4 ID:SGTC_1304|Compound:1165-0502|FD-Score:4.80|P-value:3.63E-5 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:4.15|P-value:3.01E-4 ID:SGTC_1654|Compound:st012436|FD-Score:3.78|P-value:9.00E-4 ID:SGTC_1724|Compound:st037281|FD-Score:5.25|P-value:7.26E-6 ID:SGTC_1728|Compound:st035614|FD-Score:4.06|P-value:3.95E-4 ID:SGTC_1729|Compound:st035615|FD-Score:5.22|P-value:7.94E-6 ID:SGTC_1730|Compound:st033808|FD-Score:3.97|P-value:5.22E-4 ID:SGTC_1735|Compound:st034310|FD-Score:4.33|P-value:1.76E-4 ID:SGTC_1775|Compound:st081588|FD-Score:-4.04|P-value:4.15E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-5.14|P-value:1.08E-5 ID:SGTC_1812|Compound:st052057|FD-Score:3.78|P-value:9.04E-4 ID:SGTC_1851|Compound:st056236|FD-Score:4.62|P-value:6.65E-5 ID:SGTC_1962|Compound:st076595|FD-Score:4.40|P-value:1.39E-4 ID:SGTC_2007|Compound:4011539|FD-Score:5.07|P-value:1.42E-5 ID:SGTC_2128|Compound:5255449|FD-Score:-5.87|P-value:6.06E-7 ID:SGTC_2147|Compound:5545932|FD-Score:3.88|P-value:6.66E-4 ID:SGTC_2165|Compound:5727197|FD-Score:4.71|P-value:4.89E-5 ID:SGTC_2224|Compound:6650123|FD-Score:4.70|P-value:5.18E-5 ID:SGTC_2315|Compound:7733312|FD-Score:-4.59|P-value:7.36E-5 ID:SGTC_2358|Compound:9037157|FD-Score:-3.74|P-value:9.91E-4 ID:SGTC_2377|Compound:9073351|FD-Score:7.72|P-value:8.82E-11 ID:SGTC_2514|Compound:salinomycin|FD-Score:-4.12|P-value:3.27E-4 ID:SGTC_2525|Compound:harmine|FD-Score:4.51|P-value:9.80E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:3.82|P-value:8.03E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-7.03|P-value:3.15E-9 ID:SGTC_2688|Compound:kanamycin b|FD-Score:9.11|P-value:2.65E-14 ID:SGTC_2730|Compound:imipramine|FD-Score:4.26|P-value:2.17E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:4.96|P-value:2.05E-5 ID:SGTC_2860|Compound:9025725|FD-Score:-3.99|P-value:4.81E-4 ID:SGTC_2871|Compound:9038510|FD-Score:3.78|P-value:8.86E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-3.76|P-value:9.51E-4 ID:SGTC_2879|Compound:9041815|FD-Score:-4.22|P-value:2.45E-4 ID:SGTC_2883|Compound:9059145|FD-Score:4.02|P-value:4.52E-4 ID:SGTC_2896|Compound:9052656|FD-Score:7.91|P-value:3.10E-11 ID:SGTC_3237|Compound:9133362|FD-Score:4.44|P-value:1.20E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-6.81|P-value:8.88E-9 ID:SGTC_3284|Compound:9108171|FD-Score:4.37|P-value:1.53E-4 ID:SGTC_3352|Compound:9151559|FD-Score:4.16|P-value:2.97E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:6.22|P-value:1.40E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:-6.50|P-value:3.88E-8 ID:SGTC_61|Compound:0978-8327|FD-Score:5.18|P-value:9.43E-6 ID:SGTC_103|Compound:0844-0104|FD-Score:-3.92|P-value:5.92E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:5.07|P-value:1.39E-5 ID:SGTC_187|Compound:1488-2104|FD-Score:3.96|P-value:5.28E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.39|P-value:1.41E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:4.22|P-value:2.47E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.92|P-value:5.96E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:9.01|P-value:4.71E-14 ID:SGTC_514|Compound:4466-0038|FD-Score:-4.05|P-value:4.08E-4 ID:SGTC_518|Compound:1021-0119|FD-Score:6.09|P-value:2.46E-7 ID:SGTC_528|Compound:3492-0019|FD-Score:9.33|P-value:6.21E-15 ID:SGTC_542|Compound:3381-0244|FD-Score:5.09|P-value:1.31E-5 ID:SGTC_562|Compound:3772-2733|FD-Score:4.45|P-value:1.19E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:5.32|P-value:5.51E-6 ID:SGTC_658|Compound:3253-0951|FD-Score:6.32|P-value:8.92E-8 ID:SGTC_708|Compound:3448-5400|FD-Score:4.26|P-value:2.14E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:4.28|P-value:2.04E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.71|P-value:1.20E-6 ID:SGTC_830|Compound:4076-0286|FD-Score:6.83|P-value:8.43E-9 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:4.51|P-value:9.80E-5 ID:SGTC_1055|Compound:3448-0672|FD-Score:5.74|P-value:1.03E-6 ID:SGTC_1090|Compound:efavirenz|FD-Score:4.56|P-value:8.30E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:-5.58|P-value:2.01E-6 ID:SGTC_1189|Compound:1431-2119|FD-Score:6.30|P-value:9.62E-8 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.85|P-value:7.43E-4 ID:SGTC_1206|Compound:3978-0017|FD-Score:7.65|P-value:1.31E-10 ID:SGTC_1212|Compound:0520-0165|FD-Score:9.76|P-value:3.74E-16 ID:SGTC_1252|Compound:0493-0875|FD-Score:4.35|P-value:1.62E-4 ID:SGTC_1304|Compound:1165-0502|FD-Score:4.80|P-value:3.63E-5 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:4.15|P-value:3.01E-4 ID:SGTC_1654|Compound:st012436|FD-Score:3.78|P-value:9.00E-4 ID:SGTC_1724|Compound:st037281|FD-Score:5.25|P-value:7.26E-6 ID:SGTC_1728|Compound:st035614|FD-Score:4.06|P-value:3.95E-4 ID:SGTC_1729|Compound:st035615|FD-Score:5.22|P-value:7.94E-6 ID:SGTC_1730|Compound:st033808|FD-Score:3.97|P-value:5.22E-4 ID:SGTC_1735|Compound:st034310|FD-Score:4.33|P-value:1.76E-4 ID:SGTC_1775|Compound:st081588|FD-Score:-4.04|P-value:4.15E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-5.14|P-value:1.08E-5 ID:SGTC_1812|Compound:st052057|FD-Score:3.78|P-value:9.04E-4 ID:SGTC_1851|Compound:st056236|FD-Score:4.62|P-value:6.65E-5 ID:SGTC_1962|Compound:st076595|FD-Score:4.40|P-value:1.39E-4 ID:SGTC_2007|Compound:4011539|FD-Score:5.07|P-value:1.42E-5 ID:SGTC_2128|Compound:5255449|FD-Score:-5.87|P-value:6.06E-7 ID:SGTC_2147|Compound:5545932|FD-Score:3.88|P-value:6.66E-4 ID:SGTC_2165|Compound:5727197|FD-Score:4.71|P-value:4.89E-5 ID:SGTC_2224|Compound:6650123|FD-Score:4.70|P-value:5.18E-5 ID:SGTC_2315|Compound:7733312|FD-Score:-4.59|P-value:7.36E-5 ID:SGTC_2358|Compound:9037157|FD-Score:-3.74|P-value:9.91E-4 ID:SGTC_2377|Compound:9073351|FD-Score:7.72|P-value:8.82E-11 ID:SGTC_2514|Compound:salinomycin|FD-Score:-4.12|P-value:3.27E-4 ID:SGTC_2525|Compound:harmine|FD-Score:4.51|P-value:9.80E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:3.82|P-value:8.03E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-7.03|P-value:3.15E-9 ID:SGTC_2688|Compound:kanamycin b|FD-Score:9.11|P-value:2.65E-14 ID:SGTC_2730|Compound:imipramine|FD-Score:4.26|P-value:2.17E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:4.96|P-value:2.05E-5 ID:SGTC_2860|Compound:9025725|FD-Score:-3.99|P-value:4.81E-4 ID:SGTC_2871|Compound:9038510|FD-Score:3.78|P-value:8.86E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-3.76|P-value:9.51E-4 ID:SGTC_2879|Compound:9041815|FD-Score:-4.22|P-value:2.45E-4 ID:SGTC_2883|Compound:9059145|FD-Score:4.02|P-value:4.52E-4 ID:SGTC_2896|Compound:9052656|FD-Score:7.91|P-value:3.10E-11 ID:SGTC_3237|Compound:9133362|FD-Score:4.44|P-value:1.20E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-6.81|P-value:8.88E-9 ID:SGTC_3284|Compound:9108171|FD-Score:4.37|P-value:1.53E-4 ID:SGTC_3352|Compound:9151559|FD-Score:4.16|P-value:2.97E-4

Top fitness defect scores for YOR067C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1212 0520-0165 9.76 585.0 µM 3.74E-16 1 8.68E-23
2 SGTC_528 3492-0019 9.33 172.0 µM 6.21E-15 calcium & mitochondrial duress 46 5.21E-21
3 SGTC_2688 kanamycin b 9.11 96.7 µM 2.65E-14 calcium & mitochondrial duress 42 4.31E-20
4 SGTC_513 4245-1575 9.01 41.7 µM 4.71E-14 unfolded protein response 20 9.98E-20
5 SGTC_2896 9052656 7.91 71.4 µM 3.10E-11 endomembrane recycling 20 1.26E-15
6 SGTC_2377 9073351 7.72 62.6 µM 8.82E-11 ergosterol biosynthesis 2 5.76E-15
7 SGTC_1206 3978-0017 7.65 132.0 µM 1.31E-10 ERG2 5 1.03E-14
8 SGTC_830 4076-0286 6.83 57.8 µM 8.43E-9 translation 6 4.34E-12
9 SGTC_658 3253-0951 6.32 102.0 µM 8.92E-8 calcium & mitochondrial duress 11 1.33E-10
10 SGTC_1189 1431-2119 6.30 7.3 µM 9.62E-8 3 1.48E-10
11 SGTC_56 0957-0365 6.22 83.2 µM 1.40E-7 Golgi 15 2.56E-10
12 SGTC_518 1021-0119 6.09 62.6 µM 2.46E-7 ERG2 10 5.77E-10
13 SGTC_1055 3448-0672 5.74 86.0 µM 1.03E-6 12 4.62E-9
14 SGTC_800 3448-7362 5.71 155.0 µM 1.20E-6 translation 59 5.74E-9
15 SGTC_625 k213-0058 5.32 121.0 µM 5.51E-6 translation 50 5.18E-8
16 SGTC_1724 st037281 5.25 8.3 µM 7.26E-6 15 7.73E-8
17 SGTC_1729 st035615 5.22 46.0 µM 7.94E-6 14 8.80E-8
18 SGTC_61 0978-8327 5.18 98.6 µM 9.43E-6 ergosterol biosynthesis 10 1.13E-7
19 SGTC_542 3381-0244 5.09 89.3 µM 1.31E-5 18 1.80E-7
20 SGTC_143 4032-1194 5.07 15.7 µM 1.39E-5 30 1.97E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.299 2.80E-70 YOR002W ALG6 hom Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
0.190 9.80E-29 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.180 7.43E-26 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.176 7.35E-25 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.174 2.72E-24 YMR099C YMR099C hom Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
0.158 3.19E-20 YOL081W IRA2 hom GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication
0.155 2.17E-19 YBL082C ALG3 hom Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.154 2.80E-19 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.152 9.20E-19 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.151 1.21E-18 YJR088C EMC2 hom Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35
0.140 3.23E-16 YML007C-A_p YML007C-A_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria
0.139 5.31E-16 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.138 9.29E-16 YJL129C TRK1 hom Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
0.137 1.65E-15 YBL083C_d YBL083C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3
0.134 6.39E-15 YGL079W_p KXD1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome