HIPHOP chemogenomics database

YOR067C / ALG8

Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p

Zygosity: Homozygous strain

fixedexpanded
Profile for YOR067C / ALG8

Click on Significant Values for Screen Details

Top fitness defect scores for YOR067C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1212	0520-0165	9.76	585.0 µM	3.74E-16		1	8.68E-23
2	SGTC_528	3492-0019	9.33	172.0 µM	6.21E-15	calcium & mitochondrial duress	46	5.21E-21
3	SGTC_2688	kanamycin b	9.11	96.7 µM	2.65E-14	calcium & mitochondrial duress	42	4.31E-20
4	SGTC_513	4245-1575	9.01	41.7 µM	4.71E-14	unfolded protein response	20	9.98E-20
5	SGTC_2896	9052656	7.91	71.4 µM	3.10E-11	endomembrane recycling	20	1.26E-15
6	SGTC_2377	9073351	7.72	62.6 µM	8.82E-11	ergosterol biosynthesis	2	5.76E-15
7	SGTC_1206	3978-0017	7.65	132.0 µM	1.31E-10	ERG2	5	1.03E-14
8	SGTC_830	4076-0286	6.83	57.8 µM	8.43E-9	translation	6	4.34E-12
9	SGTC_658	3253-0951	6.32	102.0 µM	8.92E-8	calcium & mitochondrial duress	11	1.33E-10
10	SGTC_1189	1431-2119	6.30	7.3 µM	9.62E-8		3	1.48E-10
11	SGTC_56	0957-0365	6.22	83.2 µM	1.40E-7	Golgi	15	2.56E-10
12	SGTC_518	1021-0119	6.09	62.6 µM	2.46E-7	ERG2	10	5.77E-10
13	SGTC_1055	3448-0672	5.74	86.0 µM	1.03E-6		12	4.62E-9
14	SGTC_800	3448-7362	5.71	155.0 µM	1.20E-6	translation	59	5.74E-9
15	SGTC_625	k213-0058	5.32	121.0 µM	5.51E-6	translation	50	5.18E-8
16	SGTC_1724	st037281	5.25	8.3 µM	7.26E-6		15	7.73E-8
17	SGTC_1729	st035615	5.22	46.0 µM	7.94E-6		14	8.80E-8
18	SGTC_61	0978-8327	5.18	98.6 µM	9.43E-6	ergosterol biosynthesis	10	1.13E-7
19	SGTC_542	3381-0244	5.09	89.3 µM	1.31E-5		18	1.80E-7
20	SGTC_143	4032-1194	5.07	15.7 µM	1.39E-5		30	1.97E-7

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.299	2.80E-70	YOR002W	ALG6	hom	Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
0.190	9.80E-29	YDR475C	JIP4	hom	Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.180	7.43E-26	YOR085W	OST3	hom	Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.176	7.35E-25	YDR467C_d	YDR467C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.174	2.72E-24	YMR099C	YMR099C	hom	Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
0.158	3.19E-20	YOL081W	IRA2	hom	GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication
0.155	2.17E-19	YBL082C	ALG3	hom	Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.154	2.80E-19	YDR466W	PKH3	hom	Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.152	9.20E-19	YJL165C	HAL5	hom	Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.151	1.21E-18	YJR088C	EMC2	hom	Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35
0.140	3.23E-16	YML007C-A_p	YML007C-A_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria
0.139	5.31E-16	YDL096C_d	OPI6_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.138	9.29E-16	YJL129C	TRK1	hom	Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
0.137	1.65E-15	YBL083C_d	YBL083C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3
0.134	6.39E-15	YGL079W_p	KXD1_p	hom	Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome