YOR069W / VPS5

Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR069W / VPS5

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-5.02|P-value:8.64E-7 ID:SGTC_70|Compound:0141-0289|FD-Score:3.60|P-value:3.33E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:7.43|P-value:9.67E-13 ID:SGTC_124|Compound:0136-0217|FD-Score:4.92|P-value:1.67E-6 ID:SGTC_184|Compound:1494-0393|FD-Score:4.68|P-value:4.82E-6 ID:SGTC_263|Compound:alizarin|FD-Score:4.40|P-value:1.62E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.99|P-value:7.10E-5 ID:SGTC_526|Compound:4469-0568|FD-Score:3.43|P-value:6.07E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:5.68|P-value:3.77E-8 ID:SGTC_577|Compound:r071-0003|FD-Score:4.60|P-value:6.95E-6 ID:SGTC_733|Compound:4069-0007|FD-Score:4.52|P-value:9.71E-6 ID:SGTC_743|Compound:1275-0778|FD-Score:-3.30|P-value:8.06E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.55|P-value:3.54E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:3.46|P-value:5.42E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:-5.38|P-value:1.49E-7 ID:SGTC_994|Compound:3910-0338|FD-Score:4.53|P-value:9.44E-6 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:-3.52|P-value:3.88E-4 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:3.82|P-value:1.55E-4 ID:SGTC_1084|Compound:voriconazole|FD-Score:6.05|P-value:5.17E-9 ID:SGTC_1115|Compound:galangin|FD-Score:3.49|P-value:4.85E-4 ID:SGTC_1146|Compound:3579-1023|FD-Score:-3.59|P-value:3.11E-4 ID:SGTC_1304|Compound:1165-0502|FD-Score:3.95|P-value:9.55E-5 ID:SGTC_1382|Compound:3002-2117|FD-Score:3.81|P-value:1.60E-4 ID:SGTC_1400|Compound:3771-8998|FD-Score:4.11|P-value:5.18E-5 ID:SGTC_1754|Compound:st042065|FD-Score:-3.91|P-value:9.77E-5 ID:SGTC_1766|Compound:st045414|FD-Score:5.09|P-value:7.51E-7 ID:SGTC_1844|Compound:rapamycin|FD-Score:8.51|P-value:3.68E-16 ID:SGTC_1886|Compound:st057356|FD-Score:4.87|P-value:2.02E-6 ID:SGTC_1920|Compound:st075728|FD-Score:4.46|P-value:1.23E-5 ID:SGTC_1937|Compound:st074714|FD-Score:3.59|P-value:3.48E-4 ID:SGTC_1943|Compound:st076331|FD-Score:-3.90|P-value:1.02E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.44|P-value:5.85E-4 ID:SGTC_2176|Compound:5809774|FD-Score:3.71|P-value:2.31E-4 ID:SGTC_2311|Compound:7694451|FD-Score:3.29|P-value:9.58E-4 ID:SGTC_2427|Compound:5545055|FD-Score:-4.38|P-value:1.46E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.88|P-value:1.24E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.10|P-value:3.94E-9 ID:SGTC_2792|Compound:7535235|FD-Score:4.93|P-value:1.54E-6 ID:SGTC_2803|Compound:7707263|FD-Score:3.43|P-value:6.10E-4 ID:SGTC_2805|Compound:7950783|FD-Score:3.64|P-value:2.98E-4 ID:SGTC_3143|Compound:9086658|FD-Score:-3.55|P-value:3.49E-4 ID:SGTC_3152|Compound:9099253|FD-Score:4.53|P-value:9.19E-6 ID:SGTC_3216|Compound:9129716|FD-Score:5.62|P-value:5.41E-8 ID:SGTC_3238|Compound:9133470|FD-Score:3.31|P-value:8.80E-4 ID:SGTC_3335|Compound:9143315|FD-Score:3.70|P-value:2.38E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.02|P-value:8.64E-7 ID:SGTC_70|Compound:0141-0289|FD-Score:3.60|P-value:3.33E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:7.43|P-value:9.67E-13 ID:SGTC_124|Compound:0136-0217|FD-Score:4.92|P-value:1.67E-6 ID:SGTC_184|Compound:1494-0393|FD-Score:4.68|P-value:4.82E-6 ID:SGTC_263|Compound:alizarin|FD-Score:4.40|P-value:1.62E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.99|P-value:7.10E-5 ID:SGTC_526|Compound:4469-0568|FD-Score:3.43|P-value:6.07E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:5.68|P-value:3.77E-8 ID:SGTC_577|Compound:r071-0003|FD-Score:4.60|P-value:6.95E-6 ID:SGTC_733|Compound:4069-0007|FD-Score:4.52|P-value:9.71E-6 ID:SGTC_743|Compound:1275-0778|FD-Score:-3.30|P-value:8.06E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.55|P-value:3.54E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:3.46|P-value:5.42E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:-5.38|P-value:1.49E-7 ID:SGTC_994|Compound:3910-0338|FD-Score:4.53|P-value:9.44E-6 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:-3.52|P-value:3.88E-4 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:3.82|P-value:1.55E-4 ID:SGTC_1084|Compound:voriconazole|FD-Score:6.05|P-value:5.17E-9 ID:SGTC_1115|Compound:galangin|FD-Score:3.49|P-value:4.85E-4 ID:SGTC_1146|Compound:3579-1023|FD-Score:-3.59|P-value:3.11E-4 ID:SGTC_1304|Compound:1165-0502|FD-Score:3.95|P-value:9.55E-5 ID:SGTC_1382|Compound:3002-2117|FD-Score:3.81|P-value:1.60E-4 ID:SGTC_1400|Compound:3771-8998|FD-Score:4.11|P-value:5.18E-5 ID:SGTC_1754|Compound:st042065|FD-Score:-3.91|P-value:9.77E-5 ID:SGTC_1766|Compound:st045414|FD-Score:5.09|P-value:7.51E-7 ID:SGTC_1844|Compound:rapamycin|FD-Score:8.51|P-value:3.68E-16 ID:SGTC_1886|Compound:st057356|FD-Score:4.87|P-value:2.02E-6 ID:SGTC_1920|Compound:st075728|FD-Score:4.46|P-value:1.23E-5 ID:SGTC_1937|Compound:st074714|FD-Score:3.59|P-value:3.48E-4 ID:SGTC_1943|Compound:st076331|FD-Score:-3.90|P-value:1.02E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.44|P-value:5.85E-4 ID:SGTC_2176|Compound:5809774|FD-Score:3.71|P-value:2.31E-4 ID:SGTC_2311|Compound:7694451|FD-Score:3.29|P-value:9.58E-4 ID:SGTC_2427|Compound:5545055|FD-Score:-4.38|P-value:1.46E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.88|P-value:1.24E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.10|P-value:3.94E-9 ID:SGTC_2792|Compound:7535235|FD-Score:4.93|P-value:1.54E-6 ID:SGTC_2803|Compound:7707263|FD-Score:3.43|P-value:6.10E-4 ID:SGTC_2805|Compound:7950783|FD-Score:3.64|P-value:2.98E-4 ID:SGTC_3143|Compound:9086658|FD-Score:-3.55|P-value:3.49E-4 ID:SGTC_3152|Compound:9099253|FD-Score:4.53|P-value:9.19E-6 ID:SGTC_3216|Compound:9129716|FD-Score:5.62|P-value:5.41E-8 ID:SGTC_3238|Compound:9133470|FD-Score:3.31|P-value:8.80E-4 ID:SGTC_3335|Compound:9143315|FD-Score:3.70|P-value:2.38E-4

Top fitness defect scores for YOR069W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1844 rapamycin 8.51 4.0 nM 3.68E-16 48 8.34E-18
2 SGTC_117 lucigenin 7.43 22.1 µM 9.67E-13 redox potentiating 25 5.24E-14
3 SGTC_2679 rapamycin 6.10 1.0 nM 3.94E-9 73 5.35E-10
4 SGTC_1084 voriconazole 6.05 435.0 nM 5.17E-9 azole & statin 7 7.23E-10
5 SGTC_528 3492-0019 5.68 172.0 µM 3.77E-8 calcium & mitochondrial duress 131 6.56E-9
6 SGTC_3216 9129716 5.62 49.5 µM 5.41E-8 RPP1 & pyrimidine depletion 8 9.79E-9
7 SGTC_1766 st045414 5.09 44.8 µM 7.51E-7 RPP1 & pyrimidine depletion 11 1.82E-7
8 SGTC_2792 7535235 4.93 81.8 µM 1.54E-6 fatty acid desaturase (OLE1) 16 4.03E-7
9 SGTC_124 0136-0217 4.92 7.1 µM 1.67E-6 mitochondrial stress 34 4.40E-7
10 SGTC_1886 st057356 4.87 49.9 µM 2.02E-6 60S ribosome export 5 5.45E-7
11 SGTC_184 1494-0393 4.68 24.7 µM 4.82E-6 43 1.43E-6
12 SGTC_577 r071-0003 4.60 38.6 µM 6.95E-6 fatty acid desaturase (OLE1) 36 2.15E-6
13 SGTC_3152 9099253 4.53 49.5 µM 9.19E-6 NEO1-PIK1 102 2.93E-6
14 SGTC_994 3910-0338 4.53 138.0 µM 9.44E-6 calcium & mitochondrial duress 29 3.02E-6
15 SGTC_733 4069-0007 4.52 56.1 µM 9.71E-6 ERAD & cell cycle 71 3.11E-6
16 SGTC_1920 st075728 4.46 45.7 µM 1.23E-5 9 4.04E-6
17 SGTC_263 alizarin 4.40 107.9 µM 1.62E-5 iron homeostasis 45 5.51E-6
18 SGTC_1400 3771-8998 4.11 51.5 µM 5.18E-5 7 1.99E-5
19 SGTC_1304 1165-0502 3.95 251.0 µM 9.55E-5 58 3.92E-5
20 SGTC_2640 pentamidine 3.88 10.4 µM 1.24E-4 mitochondrial processes 36 5.23E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.260 8.25E-53 YOR068C VAM10 hom Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering
0.251 2.94E-49 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.220 5.55E-38 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.200 1.73E-31 YNL119W NCS2 hom Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.199 2.39E-31 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.195 6.48E-30 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.187 1.02E-27 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.182 2.84E-26 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.181 6.34E-26 YFL024C EPL1 het Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
0.179 1.42E-25 YJL164C TPK1 hom cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
0.177 4.34E-25 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.177 6.65E-25 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.175 1.54E-24 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.173 7.47E-24 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.171 1.82E-23 YOR274W MOD5 hom Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis