YOR085W / OST3

Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR085W / OST3

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:3.35|P-value:3.66E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:3.25|P-value:5.27E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:6.03|P-value:5.96E-10 ID:SGTC_117|Compound:lucigenin|FD-Score:4.74|P-value:8.86E-7 ID:SGTC_143|Compound:4032-1194|FD-Score:3.10|P-value:8.65E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:3.44|P-value:2.62E-4 ID:SGTC_193|Compound:1273-0060|FD-Score:-3.58|P-value:1.60E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.30|P-value:4.49E-8 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.62|P-value:1.62E-6 ID:SGTC_314|Compound:1671-0008|FD-Score:-3.10|P-value:9.14E-4 ID:SGTC_337|Compound:1315-0038|FD-Score:3.09|P-value:9.07E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:4.12|P-value:1.61E-5 ID:SGTC_533|Compound:1222-0222|FD-Score:-6.59|P-value:1.62E-11 ID:SGTC_539|Compound:4859-0462|FD-Score:-5.02|P-value:2.19E-7 ID:SGTC_541|Compound:4513-1321|FD-Score:-3.84|P-value:5.62E-5 ID:SGTC_581|Compound:0958-0271|FD-Score:3.67|P-value:1.05E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:11.70|P-value:2.12E-32 ID:SGTC_663|Compound:1486-0733|FD-Score:5.06|P-value:1.64E-7 ID:SGTC_691|Compound:1548-0686|FD-Score:3.12|P-value:8.16E-4 ID:SGTC_697|Compound:0508-1955|FD-Score:3.09|P-value:8.97E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:14.00|P-value:2.62E-45 ID:SGTC_769|Compound:0302-0231|FD-Score:-3.36|P-value:3.56E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:8.83|P-value:2.69E-19 ID:SGTC_800|Compound:3448-7362|FD-Score:10.00|P-value:2.59E-24 ID:SGTC_830|Compound:4076-0286|FD-Score:9.68|P-value:8.23E-23 ID:SGTC_831|Compound:k061-1017|FD-Score:3.55|P-value:1.69E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:3.87|P-value:4.68E-5 ID:SGTC_849|Compound:0386-0493|FD-Score:-3.40|P-value:3.08E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.08|P-value:9.30E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:5.16|P-value:9.79E-8 ID:SGTC_1482|Compound:3346-2049|FD-Score:4.08|P-value:1.92E-5 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:-3.28|P-value:4.84E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.20|P-value:6.20E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:3.99|P-value:2.82E-5 ID:SGTC_1754|Compound:st042065|FD-Score:3.15|P-value:7.42E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-3.84|P-value:5.68E-5 ID:SGTC_1856|Compound:st057054|FD-Score:4.06|P-value:2.10E-5 ID:SGTC_1874|Compound:staurosporine|FD-Score:6.00|P-value:7.13E-10 ID:SGTC_2007|Compound:4011539|FD-Score:3.13|P-value:8.04E-4 ID:SGTC_2009|Compound:4012297|FD-Score:3.91|P-value:4.02E-5 ID:SGTC_2050|Compound:5263056|FD-Score:4.09|P-value:1.83E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-3.22|P-value:6.01E-4 ID:SGTC_2136|Compound:5328288|FD-Score:-4.48|P-value:3.23E-6 ID:SGTC_2242|Compound:6630449|FD-Score:3.79|P-value:6.42E-5 ID:SGTC_2272|Compound:7805186|FD-Score:4.25|P-value:9.11E-6 ID:SGTC_2274|Compound:7947094|FD-Score:-3.23|P-value:5.76E-4 ID:SGTC_2286|Compound:7945697|FD-Score:3.55|P-value:1.72E-4 ID:SGTC_2289|Compound:7946404|FD-Score:11.40|P-value:4.91E-31 ID:SGTC_2397|Compound:5161689|FD-Score:3.43|P-value:2.65E-4 ID:SGTC_2399|Compound:5884133|FD-Score:6.55|P-value:2.01E-11 ID:SGTC_2404|Compound:5161160|FD-Score:-3.13|P-value:8.30E-4 ID:SGTC_2439|Compound:5492030|FD-Score:3.39|P-value:3.11E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.79|P-value:6.85E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-5.90|P-value:1.39E-9 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.81|P-value:6.06E-5 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:-4.05|P-value:2.32E-5 ID:SGTC_2756|Compound:carbadox|FD-Score:-5.73|P-value:4.00E-9 ID:SGTC_2784|Compound:7567673|FD-Score:3.37|P-value:3.31E-4 ID:SGTC_2939|Compound:9044578|FD-Score:-3.12|P-value:8.49E-4 ID:SGTC_3037|Compound:9091026|FD-Score:5.42|P-value:2.29E-8 ID:SGTC_3042|Compound:9091578|FD-Score:3.18|P-value:6.68E-4 ID:SGTC_3152|Compound:9099253|FD-Score:3.17|P-value:6.79E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:3.35|P-value:3.66E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:3.25|P-value:5.27E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:6.03|P-value:5.96E-10 ID:SGTC_117|Compound:lucigenin|FD-Score:4.74|P-value:8.86E-7 ID:SGTC_143|Compound:4032-1194|FD-Score:3.10|P-value:8.65E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:3.44|P-value:2.62E-4 ID:SGTC_193|Compound:1273-0060|FD-Score:-3.58|P-value:1.60E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.30|P-value:4.49E-8 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.62|P-value:1.62E-6 ID:SGTC_314|Compound:1671-0008|FD-Score:-3.10|P-value:9.14E-4 ID:SGTC_337|Compound:1315-0038|FD-Score:3.09|P-value:9.07E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:4.12|P-value:1.61E-5 ID:SGTC_533|Compound:1222-0222|FD-Score:-6.59|P-value:1.62E-11 ID:SGTC_539|Compound:4859-0462|FD-Score:-5.02|P-value:2.19E-7 ID:SGTC_541|Compound:4513-1321|FD-Score:-3.84|P-value:5.62E-5 ID:SGTC_581|Compound:0958-0271|FD-Score:3.67|P-value:1.05E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:11.70|P-value:2.12E-32 ID:SGTC_663|Compound:1486-0733|FD-Score:5.06|P-value:1.64E-7 ID:SGTC_691|Compound:1548-0686|FD-Score:3.12|P-value:8.16E-4 ID:SGTC_697|Compound:0508-1955|FD-Score:3.09|P-value:8.97E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:14.00|P-value:2.62E-45 ID:SGTC_769|Compound:0302-0231|FD-Score:-3.36|P-value:3.56E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:8.83|P-value:2.69E-19 ID:SGTC_800|Compound:3448-7362|FD-Score:10.00|P-value:2.59E-24 ID:SGTC_830|Compound:4076-0286|FD-Score:9.68|P-value:8.23E-23 ID:SGTC_831|Compound:k061-1017|FD-Score:3.55|P-value:1.69E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:3.87|P-value:4.68E-5 ID:SGTC_849|Compound:0386-0493|FD-Score:-3.40|P-value:3.08E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.08|P-value:9.30E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:5.16|P-value:9.79E-8 ID:SGTC_1482|Compound:3346-2049|FD-Score:4.08|P-value:1.92E-5 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:-3.28|P-value:4.84E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.20|P-value:6.20E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:3.99|P-value:2.82E-5 ID:SGTC_1754|Compound:st042065|FD-Score:3.15|P-value:7.42E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-3.84|P-value:5.68E-5 ID:SGTC_1856|Compound:st057054|FD-Score:4.06|P-value:2.10E-5 ID:SGTC_1874|Compound:staurosporine|FD-Score:6.00|P-value:7.13E-10 ID:SGTC_2007|Compound:4011539|FD-Score:3.13|P-value:8.04E-4 ID:SGTC_2009|Compound:4012297|FD-Score:3.91|P-value:4.02E-5 ID:SGTC_2050|Compound:5263056|FD-Score:4.09|P-value:1.83E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-3.22|P-value:6.01E-4 ID:SGTC_2136|Compound:5328288|FD-Score:-4.48|P-value:3.23E-6 ID:SGTC_2242|Compound:6630449|FD-Score:3.79|P-value:6.42E-5 ID:SGTC_2272|Compound:7805186|FD-Score:4.25|P-value:9.11E-6 ID:SGTC_2274|Compound:7947094|FD-Score:-3.23|P-value:5.76E-4 ID:SGTC_2286|Compound:7945697|FD-Score:3.55|P-value:1.72E-4 ID:SGTC_2289|Compound:7946404|FD-Score:11.40|P-value:4.91E-31 ID:SGTC_2397|Compound:5161689|FD-Score:3.43|P-value:2.65E-4 ID:SGTC_2399|Compound:5884133|FD-Score:6.55|P-value:2.01E-11 ID:SGTC_2404|Compound:5161160|FD-Score:-3.13|P-value:8.30E-4 ID:SGTC_2439|Compound:5492030|FD-Score:3.39|P-value:3.11E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.79|P-value:6.85E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-5.90|P-value:1.39E-9 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.81|P-value:6.06E-5 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:-4.05|P-value:2.32E-5 ID:SGTC_2756|Compound:carbadox|FD-Score:-5.73|P-value:4.00E-9 ID:SGTC_2784|Compound:7567673|FD-Score:3.37|P-value:3.31E-4 ID:SGTC_2939|Compound:9044578|FD-Score:-3.12|P-value:8.49E-4 ID:SGTC_3037|Compound:9091026|FD-Score:5.42|P-value:2.29E-8 ID:SGTC_3042|Compound:9091578|FD-Score:3.18|P-value:6.68E-4 ID:SGTC_3152|Compound:9099253|FD-Score:3.17|P-value:6.79E-4

Top fitness defect scores for YOR085W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_708 3448-5400 14.00 146.0 µM 2.62E-45 1 1.28E-44
2 SGTC_625 k213-0058 11.70 121.0 µM 2.12E-32 translation 3 6.55E-32
3 SGTC_2289 7946404 11.40 165.1 µM 4.91E-31 5 1.44E-30
4 SGTC_800 3448-7362 10.00 155.0 µM 2.59E-24 translation 7 6.00E-24
5 SGTC_830 4076-0286 9.68 57.8 µM 8.23E-23 translation 1 1.80E-22
6 SGTC_791 1348-1292 8.83 225.0 µM 2.69E-19 2 5.18E-19
7 SGTC_2399 5884133 6.55 200.0 µM 2.01E-11 cell wall signaling 24 2.93E-11
8 SGTC_70 0141-0289 6.03 43.3 µM 5.96E-10 mitochondrial stress 82 8.21E-10
9 SGTC_1874 staurosporine 6.00 7.6 µM 7.13E-10 cell wall signaling 11 9.80E-10
10 SGTC_3037 9091026 5.42 49.5 µM 2.29E-8 NEO1-PIK1 59 2.99E-8
11 SGTC_221 trifluoperazine 5.30 10.3 µM 4.49E-8 NEO1-PIK1 152 5.78E-8
12 SGTC_1434 4073-0095 5.16 163.0 µM 9.79E-8 4 1.25E-7
13 SGTC_663 1486-0733 5.06 13.9 µM 1.64E-7 cell wall signaling 29 2.07E-7
14 SGTC_117 lucigenin 4.74 22.1 µM 8.86E-7 redox potentiating 65 1.09E-6
15 SGTC_2272 7805186 4.25 180.0 µM 9.11E-6 cell wall signaling 17 1.08E-5
16 SGTC_410 α-linolenic acid 4.12 100.0 µM 1.61E-5 excess fatty acid 33 1.90E-5
17 SGTC_2050 5263056 4.09 171.0 µM 1.83E-5 fatty acid desaturase (OLE1) 38 2.14E-5
18 SGTC_1482 3346-2049 4.08 8.5 µM 1.92E-5 27 2.25E-5
19 SGTC_1856 st057054 4.06 55.7 µM 2.10E-5 27 2.45E-5
20 SGTC_1595 6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin 3.99 67.5 µM 2.82E-5 37 3.28E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.430 3.02E-151 YAL058W CNE1 hom Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
0.382 6.24E-117 YNL322C KRE1 hom Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.371 6.94E-110 YAL056C-A_d YAL056C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.345 2.11E-94 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.331 2.42E-86 YKL096C-B_p YKL096C-B_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.317 2.29E-79 YGR166W TRS65 hom Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.316 1.12E-78 YMR200W ROT1 het Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
0.294 4.53E-68 YJL139C YUR1 hom Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication
0.291 1.62E-66 YBL082C ALG3 hom Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.275 2.72E-59 YDL095W PMT1 hom Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen
0.272 5.00E-58 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.266 1.96E-55 YBR229C ROT2 hom Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
0.262 8.54E-54 YOR002W ALG6 hom Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
0.249 9.28E-49 YDR525W_d API2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology
0.243 2.43E-46 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC