YOR101W / RAS1

GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR101W / RAS1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-3.15|P-value:1.54E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:3.46|P-value:3.33E-5 ID:SGTC_147|Compound:4466-0538|FD-Score:2.79|P-value:6.55E-4 ID:SGTC_179|Compound:1273-0063|FD-Score:-2.93|P-value:3.83E-4 ID:SGTC_217|Compound:ketoconazole|FD-Score:3.55|P-value:2.21E-5 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.49|P-value:1.17E-7 ID:SGTC_365|Compound:0121-0052|FD-Score:3.02|P-value:2.57E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:3.41|P-value:4.38E-5 ID:SGTC_383|Compound:2324-0395|FD-Score:2.91|P-value:3.95E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:3.59|P-value:1.81E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.02|P-value:2.69E-4 ID:SGTC_493|Compound:zm226600|FD-Score:-3.13|P-value:1.67E-4 ID:SGTC_505|Compound:gw-5074|FD-Score:-3.82|P-value:5.73E-6 ID:SGTC_547|Compound:1120-0019|FD-Score:3.26|P-value:8.93E-5 ID:SGTC_639|Compound:0833-0042|FD-Score:-4.15|P-value:9.76E-7 ID:SGTC_696|Compound:1560-0013|FD-Score:3.04|P-value:2.28E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:4.96|P-value:5.59E-9 ID:SGTC_844|Compound:0330-0132|FD-Score:-3.11|P-value:1.80E-4 ID:SGTC_857|Compound:0438-0306|FD-Score:-3.29|P-value:8.06E-5 ID:SGTC_908|Compound:3448-7491|FD-Score:5.96|P-value:3.61E-12 ID:SGTC_919|Compound:tnp00227|FD-Score:-3.93|P-value:3.22E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:3.17|P-value:1.30E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:4.01|P-value:1.92E-6 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-2.85|P-value:5.30E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:6.23|P-value:3.98E-13 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.15|P-value:1.46E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:4.52|P-value:1.02E-7 ID:SGTC_1262|Compound:0737-0114|FD-Score:2.79|P-value:6.49E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:2.75|P-value:7.84E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:-3.54|P-value:2.38E-5 ID:SGTC_1507|Compound:4075-3230|FD-Score:2.76|P-value:7.49E-4 ID:SGTC_1561|Compound:5-methoxyflavone|FD-Score:2.73|P-value:8.27E-4 ID:SGTC_1694|Compound:st024011|FD-Score:-2.71|P-value:9.41E-4 ID:SGTC_1706|Compound:st031321|FD-Score:3.20|P-value:1.14E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:3.40|P-value:4.63E-5 ID:SGTC_1866|Compound:st057638|FD-Score:3.42|P-value:4.17E-5 ID:SGTC_1871|Compound:st057448|FD-Score:2.77|P-value:7.07E-4 ID:SGTC_1998|Compound:st075797|FD-Score:2.73|P-value:8.42E-4 ID:SGTC_2029|Compound:5175081|FD-Score:-3.97|P-value:2.56E-6 ID:SGTC_2035|Compound:5154463|FD-Score:-3.11|P-value:1.84E-4 ID:SGTC_2054|Compound:5233173|FD-Score:7.53|P-value:2.47E-18 ID:SGTC_2059|Compound:5238311|FD-Score:-2.75|P-value:7.93E-4 ID:SGTC_2118|Compound:5526350|FD-Score:-2.77|P-value:7.47E-4 ID:SGTC_2144|Compound:5628481|FD-Score:2.78|P-value:6.89E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-2.86|P-value:5.12E-4 ID:SGTC_2307|Compound:7538926|FD-Score:2.97|P-value:3.08E-4 ID:SGTC_2322|Compound:7413319|FD-Score:5.91|P-value:5.41E-12 ID:SGTC_2332|Compound:7982454|FD-Score:-3.37|P-value:5.63E-5 ID:SGTC_2369|Compound:9071472|FD-Score:3.82|P-value:5.41E-6 ID:SGTC_2449|Compound:5812982|FD-Score:-3.25|P-value:9.80E-5 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:5.64|P-value:4.37E-11 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-3.03|P-value:2.50E-4 ID:SGTC_2815|Compound:7968870|FD-Score:-2.70|P-value:9.92E-4 ID:SGTC_2853|Compound:9015042|FD-Score:-2.73|P-value:8.64E-4 ID:SGTC_2881|Compound:9058953|FD-Score:-2.71|P-value:9.53E-4 ID:SGTC_2911|Compound:7942208|FD-Score:4.61|P-value:5.76E-8 ID:SGTC_2965|Compound:9082772|FD-Score:3.97|P-value:2.41E-6 ID:SGTC_2990|Compound:9074105|FD-Score:-3.09|P-value:1.98E-4 ID:SGTC_2996|Compound:9066192|FD-Score:-4.07|P-value:1.50E-6 ID:SGTC_2998|Compound:9066762|FD-Score:-2.94|P-value:3.79E-4 ID:SGTC_3171|Compound:9103192|FD-Score:-2.76|P-value:7.78E-4 ID:SGTC_3331|Compound:9141060|FD-Score:-3.48|P-value:3.23E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.15|P-value:1.54E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:3.46|P-value:3.33E-5 ID:SGTC_147|Compound:4466-0538|FD-Score:2.79|P-value:6.55E-4 ID:SGTC_179|Compound:1273-0063|FD-Score:-2.93|P-value:3.83E-4 ID:SGTC_217|Compound:ketoconazole|FD-Score:3.55|P-value:2.21E-5 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.49|P-value:1.17E-7 ID:SGTC_365|Compound:0121-0052|FD-Score:3.02|P-value:2.57E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:3.41|P-value:4.38E-5 ID:SGTC_383|Compound:2324-0395|FD-Score:2.91|P-value:3.95E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:3.59|P-value:1.81E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.02|P-value:2.69E-4 ID:SGTC_493|Compound:zm226600|FD-Score:-3.13|P-value:1.67E-4 ID:SGTC_505|Compound:gw-5074|FD-Score:-3.82|P-value:5.73E-6 ID:SGTC_547|Compound:1120-0019|FD-Score:3.26|P-value:8.93E-5 ID:SGTC_639|Compound:0833-0042|FD-Score:-4.15|P-value:9.76E-7 ID:SGTC_696|Compound:1560-0013|FD-Score:3.04|P-value:2.28E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:4.96|P-value:5.59E-9 ID:SGTC_844|Compound:0330-0132|FD-Score:-3.11|P-value:1.80E-4 ID:SGTC_857|Compound:0438-0306|FD-Score:-3.29|P-value:8.06E-5 ID:SGTC_908|Compound:3448-7491|FD-Score:5.96|P-value:3.61E-12 ID:SGTC_919|Compound:tnp00227|FD-Score:-3.93|P-value:3.22E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:3.17|P-value:1.30E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:4.01|P-value:1.92E-6 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-2.85|P-value:5.30E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:6.23|P-value:3.98E-13 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.15|P-value:1.46E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:4.52|P-value:1.02E-7 ID:SGTC_1262|Compound:0737-0114|FD-Score:2.79|P-value:6.49E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:2.75|P-value:7.84E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:-3.54|P-value:2.38E-5 ID:SGTC_1507|Compound:4075-3230|FD-Score:2.76|P-value:7.49E-4 ID:SGTC_1561|Compound:5-methoxyflavone|FD-Score:2.73|P-value:8.27E-4 ID:SGTC_1694|Compound:st024011|FD-Score:-2.71|P-value:9.41E-4 ID:SGTC_1706|Compound:st031321|FD-Score:3.20|P-value:1.14E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:3.40|P-value:4.63E-5 ID:SGTC_1866|Compound:st057638|FD-Score:3.42|P-value:4.17E-5 ID:SGTC_1871|Compound:st057448|FD-Score:2.77|P-value:7.07E-4 ID:SGTC_1998|Compound:st075797|FD-Score:2.73|P-value:8.42E-4 ID:SGTC_2029|Compound:5175081|FD-Score:-3.97|P-value:2.56E-6 ID:SGTC_2035|Compound:5154463|FD-Score:-3.11|P-value:1.84E-4 ID:SGTC_2054|Compound:5233173|FD-Score:7.53|P-value:2.47E-18 ID:SGTC_2059|Compound:5238311|FD-Score:-2.75|P-value:7.93E-4 ID:SGTC_2118|Compound:5526350|FD-Score:-2.77|P-value:7.47E-4 ID:SGTC_2144|Compound:5628481|FD-Score:2.78|P-value:6.89E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-2.86|P-value:5.12E-4 ID:SGTC_2307|Compound:7538926|FD-Score:2.97|P-value:3.08E-4 ID:SGTC_2322|Compound:7413319|FD-Score:5.91|P-value:5.41E-12 ID:SGTC_2332|Compound:7982454|FD-Score:-3.37|P-value:5.63E-5 ID:SGTC_2369|Compound:9071472|FD-Score:3.82|P-value:5.41E-6 ID:SGTC_2449|Compound:5812982|FD-Score:-3.25|P-value:9.80E-5 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:5.64|P-value:4.37E-11 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-3.03|P-value:2.50E-4 ID:SGTC_2815|Compound:7968870|FD-Score:-2.70|P-value:9.92E-4 ID:SGTC_2853|Compound:9015042|FD-Score:-2.73|P-value:8.64E-4 ID:SGTC_2881|Compound:9058953|FD-Score:-2.71|P-value:9.53E-4 ID:SGTC_2911|Compound:7942208|FD-Score:4.61|P-value:5.76E-8 ID:SGTC_2965|Compound:9082772|FD-Score:3.97|P-value:2.41E-6 ID:SGTC_2990|Compound:9074105|FD-Score:-3.09|P-value:1.98E-4 ID:SGTC_2996|Compound:9066192|FD-Score:-4.07|P-value:1.50E-6 ID:SGTC_2998|Compound:9066762|FD-Score:-2.94|P-value:3.79E-4 ID:SGTC_3171|Compound:9103192|FD-Score:-2.76|P-value:7.78E-4 ID:SGTC_3331|Compound:9141060|FD-Score:-3.48|P-value:3.23E-5

Top fitness defect scores for YOR101W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2054 5233173 7.53 129.0 µM 2.47E-18 mitochondrial response to ROS 8 2.63E-14
2 SGTC_1240 0356-0016 6.23 42.1 µM 3.98E-13 mitochondrial response to ROS 8 2.39E-10
3 SGTC_908 3448-7491 5.96 47.7 µM 3.61E-12 mitochondrial stress 50 1.28E-9
4 SGTC_2322 7413319 5.91 38.2 µM 5.41E-12 ERAD & cell cycle 66 1.74E-9
5 SGTC_2572 astragaloside iv 5.64 100.0 µM 4.37E-11 5 8.53E-9
6 SGTC_778 3456-2434 4.96 196.0 µM 5.59E-9 mitochondrial response to ROS 46 3.45E-7
7 SGTC_2911 7942208 4.61 15.6 µM 5.76E-8 mitochondrial response to ROS 9 2.04E-6
8 SGTC_1258 0671-0043 4.52 57.1 µM 1.02E-7 13 3.16E-6
9 SGTC_275 trichlorophene 4.49 5.7 µM 1.17E-7 mitochondrial stress 200 3.51E-6
10 SGTC_1123 mangostin 4.01 811.3 nM 1.92E-6 mitochondrial response to ROS 38 2.98E-5
11 SGTC_2965 9082772 3.97 38.6 µM 2.41E-6 mitochondrial response to ROS 39 3.55E-5
12 SGTC_2369 9071472 3.82 200.0 µM 5.41E-6 18 6.59E-5
13 SGTC_400 oxethazaine 3.59 37.5 µM 1.81E-5 calcium & mitochondrial duress 31 1.66E-4
14 SGTC_217 ketoconazole 3.55 989.0 nM 2.21E-5 ergosterol depletion effects on membrane 38 1.94E-4
15 SGTC_73 0327-0325 3.46 16.2 µM 3.33E-5 DNA damage response 69 2.66E-4
16 SGTC_1866 st057638 3.42 64.0 µM 4.17E-5 17 3.15E-4
17 SGTC_372 0155-0182 3.41 15.9 µM 4.38E-5 mitochondrial response to ROS 47 3.28E-4
18 SGTC_1718 roemerine 3.40 11.9 µM 4.63E-5 redox potentiating 86 3.42E-4
19 SGTC_547 1120-0019 3.26 610.0 nM 8.93E-5 azole & statin 52 5.67E-4
20 SGTC_1706 st031321 3.20 10.0 µM 1.14E-4 TSC3-RPN4 30 6.82E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.357 3.90E-101 YFL036W RPO41 hom Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.271 1.23E-57 YBR078W ECM33 hom GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p
0.206 2.08E-33 YNR015W SMM1 hom Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs
0.169 8.22E-23 YKL139W CTK1 hom Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
0.132 2.00E-14 YKL206C ADD66 hom Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly
0.130 4.94E-14 YDR115W YDR115W hom Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress
0.126 2.48E-13 YBL080C PET112 hom Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog
0.126 2.96E-13 YER125W RSP5 het E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p
0.119 5.21E-12 YPL047W SGF11 hom Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation
0.118 6.79E-12 YHR008C SOD2 hom Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated
0.115 2.18E-11 YNR037C RSM19 hom Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein
0.112 7.43E-11 YMR098C ATP25 hom Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene
0.110 1.43E-10 YGL013C PDR1 hom Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
0.110 1.91E-10 YHR042W NCP1 het NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p
0.107 4.51E-10 YBR179C FZO1 hom Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system