YOR103C / OST2

Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOR103C / OST2

Click on Significant Values for Screen Details ID:SGTC_116|Compound:0155-0182|FD-Score:-4.44|P-value:1.03E-5 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.95|P-value:7.55E-5 ID:SGTC_308|Compound:4092-1169|FD-Score:3.81|P-value:1.29E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.33|P-value:7.03E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:4.04|P-value:5.48E-5 ID:SGTC_723|Compound:4182-0001|FD-Score:4.36|P-value:1.43E-5 ID:SGTC_767|Compound:0929-0023|FD-Score:3.96|P-value:7.37E-5 ID:SGTC_771|Compound:0250-0039|FD-Score:3.26|P-value:8.87E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:3.27|P-value:8.57E-4 ID:SGTC_1143|Compound:3454-2408|FD-Score:3.84|P-value:1.16E-4 ID:SGTC_1151|Compound:3909-8316|FD-Score:5.28|P-value:2.04E-7 ID:SGTC_1206|Compound:3978-0017|FD-Score:4.19|P-value:2.94E-5 ID:SGTC_1470|Compound:k295-0292|FD-Score:3.97|P-value:6.97E-5 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.65|P-value:2.37E-4 ID:SGTC_1853|Compound:st056710|FD-Score:3.33|P-value:7.14E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.37|P-value:6.22E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-5.31|P-value:1.74E-7 ID:SGTC_2127|Compound:5253358|FD-Score:-3.27|P-value:8.43E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.42|P-value:5.30E-4 ID:SGTC_2331|Compound:7980721|FD-Score:-4.46|P-value:9.31E-6 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:6.31|P-value:7.47E-10 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:3.38|P-value:5.91E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:4.39|P-value:1.28E-5 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:3.60|P-value:2.80E-4 ID:SGTC_2654|Compound:protoveratrine a|FD-Score:3.49|P-value:4.14E-4 ID:SGTC_2808|Compound:7946468|FD-Score:-4.86|P-value:1.55E-6 ID:SGTC_2990|Compound:9074105|FD-Score:3.57|P-value:3.13E-4 ID:SGTC_3329|Compound:9141233|FD-Score:3.78|P-value:1.44E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-4.44|P-value:1.03E-5 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.95|P-value:7.55E-5 ID:SGTC_308|Compound:4092-1169|FD-Score:3.81|P-value:1.29E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.33|P-value:7.03E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:4.04|P-value:5.48E-5 ID:SGTC_723|Compound:4182-0001|FD-Score:4.36|P-value:1.43E-5 ID:SGTC_767|Compound:0929-0023|FD-Score:3.96|P-value:7.37E-5 ID:SGTC_771|Compound:0250-0039|FD-Score:3.26|P-value:8.87E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:3.27|P-value:8.57E-4 ID:SGTC_1143|Compound:3454-2408|FD-Score:3.84|P-value:1.16E-4 ID:SGTC_1151|Compound:3909-8316|FD-Score:5.28|P-value:2.04E-7 ID:SGTC_1206|Compound:3978-0017|FD-Score:4.19|P-value:2.94E-5 ID:SGTC_1470|Compound:k295-0292|FD-Score:3.97|P-value:6.97E-5 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.65|P-value:2.37E-4 ID:SGTC_1853|Compound:st056710|FD-Score:3.33|P-value:7.14E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.37|P-value:6.22E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-5.31|P-value:1.74E-7 ID:SGTC_2127|Compound:5253358|FD-Score:-3.27|P-value:8.43E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.42|P-value:5.30E-4 ID:SGTC_2331|Compound:7980721|FD-Score:-4.46|P-value:9.31E-6 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:6.31|P-value:7.47E-10 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:3.38|P-value:5.91E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:4.39|P-value:1.28E-5 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:3.60|P-value:2.80E-4 ID:SGTC_2654|Compound:protoveratrine a|FD-Score:3.49|P-value:4.14E-4 ID:SGTC_2808|Compound:7946468|FD-Score:-4.86|P-value:1.55E-6 ID:SGTC_2990|Compound:9074105|FD-Score:3.57|P-value:3.13E-4 ID:SGTC_3329|Compound:9141233|FD-Score:3.78|P-value:1.44E-4

Top fitness defect scores for YOR103C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2540 epiandrosterone 6.31 100.0 µM 7.47E-10 1 1.44E-10 1.87
2 SGTC_1151 3909-8316 5.28 15.3 µM 2.04E-7 3 6.32E-8 0.60
3 SGTC_2593 nobiletin 4.39 86.2 µM 1.28E-5 8 5.62E-6 0.08
4 SGTC_723 4182-0001 4.36 115.0 µM 1.43E-5 2 6.36E-6 0.50
5 SGTC_1206 3978-0017 4.19 132.0 µM 2.94E-5 ERG2 5 1.39E-5 0.16
6 SGTC_705 1124-0212 4.04 310.0 µM 5.48E-5 5 2.72E-5 0.04
7 SGTC_1470 k295-0292 3.97 231.0 µM 6.97E-5 4 3.53E-5 0.23
8 SGTC_767 0929-0023 3.96 86.0 µM 7.37E-5 TRP & mitochondrial translation 13 3.74E-5 0.02
9 SGTC_1143 3454-2408 3.84 5.1 µM 1.16E-4 60S ribosome export 3 6.12E-5 0.07
10 SGTC_308 4092-1169 3.81 104.0 µM 1.29E-4 plasma membrane duress 7 6.84E-5 0.01
11 SGTC_3329 9141233 3.78 27.6 µM 1.44E-4 4 7.75E-5 0.20
12 SGTC_1473 k844-0725 3.65 134.0 µM 2.37E-4 7 1.33E-4 0.09
13 SGTC_2633 5,7-dihydroxyisoflavone 3.60 100.0 µM 2.80E-4 6 1.59E-4 0.05
14 SGTC_2990 9074105 3.57 71.4 µM 3.13E-4 6 1.79E-4 0.09
15 SGTC_2654 protoveratrine a 3.49 100.0 µM 4.14E-4 8 2.43E-4 0.14
16 SGTC_2131 toluylene red 3.42 5.3 µM 5.30E-4 60S ribosome export 19 3.17E-4 0.05
17 SGTC_2553 4-methylesculetin 3.38 85.1 µM 5.91E-4 9 3.57E-4 0.03
18 SGTC_2021 4019055 3.37 173.0 µM 6.22E-4 endomembrane recycling 47 3.77E-4 0.04
19 SGTC_528 3492-0019 3.33 172.0 µM 7.03E-4 calcium & mitochondrial duress 31 4.30E-4 0.04
20 SGTC_1853 st056710 3.33 91.2 µM 7.14E-4 amide catabolism 11 4.38E-4 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.126 2.58E-13 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.120 3.09E-12 YDR390C UBA2 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.120 3.58E-12 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.119 5.53E-12 YML062C MFT1 hom Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
0.117 1.01E-11 YDR235W PRP42 het U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats
0.108 4.08E-10 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.107 4.73E-10 YDR397C NCB2 het Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta
0.105 1.09E-9 YKL152C GPM1 het Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis
0.101 4.46E-9 YGL110C CUE3 hom Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination
0.099 1.02E-8 YOL130W ALR1 het Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions
0.099 1.04E-8 YBL083C_d YBL083C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3
0.099 1.05E-8 YFR042W KEG1 het Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability
0.099 1.07E-8 YIL105C SLM1 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
0.097 1.53E-8 YKR026C GCN3 hom Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
0.097 1.53E-8 YKL122C SRP21 het Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm