YOR106W / VAM3

Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR106W / VAM3

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:4.94|P-value:7.89E-6 ID:SGTC_103|Compound:0844-0104|FD-Score:3.60|P-value:8.31E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:6.41|P-value:1.03E-8 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.50|P-value:9.93E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.58|P-value:7.75E-4 ID:SGTC_187|Compound:1488-2104|FD-Score:7.25|P-value:1.10E-10 ID:SGTC_206|Compound:4546-0025|FD-Score:3.77|P-value:5.01E-4 ID:SGTC_232|Compound:clozapine|FD-Score:5.70|P-value:3.10E-7 ID:SGTC_245|Compound:methotrexate|FD-Score:5.17|P-value:3.15E-6 ID:SGTC_301|Compound:4119-0100|FD-Score:6.42|P-value:9.84E-9 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.72|P-value:1.56E-5 ID:SGTC_327|Compound:1273-0057|FD-Score:-3.75|P-value:4.71E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:5.78|P-value:2.18E-7 ID:SGTC_435|Compound:c-PAF|FD-Score:-4.20|P-value:1.05E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:4.71|P-value:1.95E-5 ID:SGTC_841|Compound:0251-0215|FD-Score:7.04|P-value:3.62E-10 ID:SGTC_1026|Compound:k007-1105|FD-Score:4.54|P-value:3.63E-5 ID:SGTC_1027|Compound:k015-0025|FD-Score:5.58|P-value:5.40E-7 ID:SGTC_1034|Compound:k072-0165|FD-Score:3.61|P-value:8.21E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:-4.39|P-value:5.36E-5 ID:SGTC_1280|Compound:0880-0253|FD-Score:-3.73|P-value:4.95E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:5.97|P-value:8.96E-8 ID:SGTC_1291|Compound:0993-0025|FD-Score:-5.03|P-value:4.65E-6 ID:SGTC_1323|Compound:1348-1351|FD-Score:4.67|P-value:2.21E-5 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.66|P-value:7.04E-4 ID:SGTC_1404|Compound:3909-7820|FD-Score:7.69|P-value:8.24E-12 ID:SGTC_1414|Compound:3917-0015|FD-Score:4.67|P-value:2.26E-5 ID:SGTC_1426|Compound:4048-4480|FD-Score:4.02|P-value:2.22E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:13.70|P-value:1.21E-33 ID:SGTC_1495|Compound:4469-0029|FD-Score:6.12|P-value:4.22E-8 ID:SGTC_1625|Compound:st003711|FD-Score:-4.52|P-value:3.41E-5 ID:SGTC_1634|Compound:st006576|FD-Score:-4.02|P-value:1.97E-4 ID:SGTC_1640|Compound:st009078|FD-Score:-3.52|P-value:9.40E-4 ID:SGTC_1658|Compound:st012944|FD-Score:4.51|P-value:4.07E-5 ID:SGTC_1665|Compound:st014639|FD-Score:5.76|P-value:2.33E-7 ID:SGTC_1687|Compound:st020778|FD-Score:6.86|P-value:9.62E-10 ID:SGTC_1710|Compound:st031758|FD-Score:4.26|P-value:9.80E-5 ID:SGTC_1846|Compound:st056244|FD-Score:5.86|P-value:1.49E-7 ID:SGTC_1873|Compound:rapamycin|FD-Score:4.73|P-value:1.79E-5 ID:SGTC_1877|Compound:wortmannin|FD-Score:4.61|P-value:2.84E-5 ID:SGTC_1991|Compound:st073000|FD-Score:5.24|P-value:2.29E-6 ID:SGTC_1995|Compound:st072814|FD-Score:4.38|P-value:6.62E-5 ID:SGTC_2008|Compound:4012253|FD-Score:4.88|P-value:9.83E-6 ID:SGTC_2106|Compound:5357045|FD-Score:6.56|P-value:4.80E-9 ID:SGTC_2127|Compound:5253358|FD-Score:4.81|P-value:1.34E-5 ID:SGTC_2291|Compound:7917025|FD-Score:5.64|P-value:4.07E-7 ID:SGTC_2322|Compound:7413319|FD-Score:5.46|P-value:9.03E-7 ID:SGTC_2380|Compound:9035439|FD-Score:3.62|P-value:7.91E-4 ID:SGTC_2397|Compound:5161689|FD-Score:3.93|P-value:3.05E-4 ID:SGTC_2424|Compound:5537928|FD-Score:4.00|P-value:2.40E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:5.87|P-value:1.44E-7 ID:SGTC_2557|Compound:derrustone|FD-Score:3.87|P-value:3.66E-4 ID:SGTC_2560|Compound:caryophyllene|FD-Score:5.42|P-value:1.06E-6 ID:SGTC_2654|Compound:protoveratrine a|FD-Score:5.06|P-value:4.80E-6 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.13|P-value:1.55E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:4.01|P-value:2.34E-4 ID:SGTC_2782|Compound:7736369|FD-Score:12.60|P-value:1.18E-28 ID:SGTC_2812|Compound:7970281|FD-Score:3.71|P-value:6.00E-4 ID:SGTC_2890|Compound:9046729|FD-Score:4.60|P-value:2.96E-5 ID:SGTC_3049|Compound:9092223|FD-Score:5.52|P-value:6.98E-7 ID:SGTC_3080|Compound:9117754|FD-Score:5.04|P-value:5.38E-6 ID:SGTC_3148|Compound:9097893|FD-Score:5.73|P-value:2.70E-7 ID:SGTC_3151|Compound:9099821|FD-Score:4.14|P-value:1.52E-4 ID:SGTC_3155|Compound:9099265|FD-Score:8.13|P-value:5.52E-13 ID:SGTC_3191|Compound:9110352|FD-Score:3.57|P-value:9.17E-4 ID:SGTC_3197|Compound:9113331|FD-Score:3.65|P-value:7.28E-4 ID:SGTC_3203|Compound:9113543|FD-Score:-4.08|P-value:1.57E-4 ID:SGTC_3274|Compound:9138298|FD-Score:5.32|P-value:1.62E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:4.94|P-value:7.89E-6 ID:SGTC_103|Compound:0844-0104|FD-Score:3.60|P-value:8.31E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:6.41|P-value:1.03E-8 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.50|P-value:9.93E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.58|P-value:7.75E-4 ID:SGTC_187|Compound:1488-2104|FD-Score:7.25|P-value:1.10E-10 ID:SGTC_206|Compound:4546-0025|FD-Score:3.77|P-value:5.01E-4 ID:SGTC_232|Compound:clozapine|FD-Score:5.70|P-value:3.10E-7 ID:SGTC_245|Compound:methotrexate|FD-Score:5.17|P-value:3.15E-6 ID:SGTC_301|Compound:4119-0100|FD-Score:6.42|P-value:9.84E-9 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.72|P-value:1.56E-5 ID:SGTC_327|Compound:1273-0057|FD-Score:-3.75|P-value:4.71E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:5.78|P-value:2.18E-7 ID:SGTC_435|Compound:c-PAF|FD-Score:-4.20|P-value:1.05E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:4.71|P-value:1.95E-5 ID:SGTC_841|Compound:0251-0215|FD-Score:7.04|P-value:3.62E-10 ID:SGTC_1026|Compound:k007-1105|FD-Score:4.54|P-value:3.63E-5 ID:SGTC_1027|Compound:k015-0025|FD-Score:5.58|P-value:5.40E-7 ID:SGTC_1034|Compound:k072-0165|FD-Score:3.61|P-value:8.21E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:-4.39|P-value:5.36E-5 ID:SGTC_1280|Compound:0880-0253|FD-Score:-3.73|P-value:4.95E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:5.97|P-value:8.96E-8 ID:SGTC_1291|Compound:0993-0025|FD-Score:-5.03|P-value:4.65E-6 ID:SGTC_1323|Compound:1348-1351|FD-Score:4.67|P-value:2.21E-5 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.66|P-value:7.04E-4 ID:SGTC_1404|Compound:3909-7820|FD-Score:7.69|P-value:8.24E-12 ID:SGTC_1414|Compound:3917-0015|FD-Score:4.67|P-value:2.26E-5 ID:SGTC_1426|Compound:4048-4480|FD-Score:4.02|P-value:2.22E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:13.70|P-value:1.21E-33 ID:SGTC_1495|Compound:4469-0029|FD-Score:6.12|P-value:4.22E-8 ID:SGTC_1625|Compound:st003711|FD-Score:-4.52|P-value:3.41E-5 ID:SGTC_1634|Compound:st006576|FD-Score:-4.02|P-value:1.97E-4 ID:SGTC_1640|Compound:st009078|FD-Score:-3.52|P-value:9.40E-4 ID:SGTC_1658|Compound:st012944|FD-Score:4.51|P-value:4.07E-5 ID:SGTC_1665|Compound:st014639|FD-Score:5.76|P-value:2.33E-7 ID:SGTC_1687|Compound:st020778|FD-Score:6.86|P-value:9.62E-10 ID:SGTC_1710|Compound:st031758|FD-Score:4.26|P-value:9.80E-5 ID:SGTC_1846|Compound:st056244|FD-Score:5.86|P-value:1.49E-7 ID:SGTC_1873|Compound:rapamycin|FD-Score:4.73|P-value:1.79E-5 ID:SGTC_1877|Compound:wortmannin|FD-Score:4.61|P-value:2.84E-5 ID:SGTC_1991|Compound:st073000|FD-Score:5.24|P-value:2.29E-6 ID:SGTC_1995|Compound:st072814|FD-Score:4.38|P-value:6.62E-5 ID:SGTC_2008|Compound:4012253|FD-Score:4.88|P-value:9.83E-6 ID:SGTC_2106|Compound:5357045|FD-Score:6.56|P-value:4.80E-9 ID:SGTC_2127|Compound:5253358|FD-Score:4.81|P-value:1.34E-5 ID:SGTC_2291|Compound:7917025|FD-Score:5.64|P-value:4.07E-7 ID:SGTC_2322|Compound:7413319|FD-Score:5.46|P-value:9.03E-7 ID:SGTC_2380|Compound:9035439|FD-Score:3.62|P-value:7.91E-4 ID:SGTC_2397|Compound:5161689|FD-Score:3.93|P-value:3.05E-4 ID:SGTC_2424|Compound:5537928|FD-Score:4.00|P-value:2.40E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:5.87|P-value:1.44E-7 ID:SGTC_2557|Compound:derrustone|FD-Score:3.87|P-value:3.66E-4 ID:SGTC_2560|Compound:caryophyllene|FD-Score:5.42|P-value:1.06E-6 ID:SGTC_2654|Compound:protoveratrine a|FD-Score:5.06|P-value:4.80E-6 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.13|P-value:1.55E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:4.01|P-value:2.34E-4 ID:SGTC_2782|Compound:7736369|FD-Score:12.60|P-value:1.18E-28 ID:SGTC_2812|Compound:7970281|FD-Score:3.71|P-value:6.00E-4 ID:SGTC_2890|Compound:9046729|FD-Score:4.60|P-value:2.96E-5 ID:SGTC_3049|Compound:9092223|FD-Score:5.52|P-value:6.98E-7 ID:SGTC_3080|Compound:9117754|FD-Score:5.04|P-value:5.38E-6 ID:SGTC_3148|Compound:9097893|FD-Score:5.73|P-value:2.70E-7 ID:SGTC_3151|Compound:9099821|FD-Score:4.14|P-value:1.52E-4 ID:SGTC_3155|Compound:9099265|FD-Score:8.13|P-value:5.52E-13 ID:SGTC_3191|Compound:9110352|FD-Score:3.57|P-value:9.17E-4 ID:SGTC_3197|Compound:9113331|FD-Score:3.65|P-value:7.28E-4 ID:SGTC_3203|Compound:9113543|FD-Score:-4.08|P-value:1.57E-4 ID:SGTC_3274|Compound:9138298|FD-Score:5.32|P-value:1.62E-6

Top fitness defect scores for YOR106W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1446 4239-0190 13.70 62.0 µM 1.21E-33 1 3.43E-43
2 SGTC_2782 7736369 12.60 71.4 µM 1.18E-28 1 1.02E-36
3 SGTC_3155 9099265 8.13 49.5 µM 5.52E-13 1 2.16E-16
4 SGTC_1404 3909-7820 7.69 48.3 µM 8.24E-12 60S ribosome export 2 7.16E-15
5 SGTC_187 1488-2104 7.25 32.7 µM 1.10E-10 calcium & mitochondrial duress 10 2.05E-13
6 SGTC_841 0251-0215 7.04 91.2 µM 3.62E-10 28 9.62E-13
7 SGTC_1687 st020778 6.86 55.4 µM 9.62E-10 90 3.40E-12
8 SGTC_2106 5357045 6.56 167.3 µM 4.80E-9 3 2.72E-11
9 SGTC_301 4119-0100 6.42 24.5 µM 9.84E-9 NEO1 34 6.90E-11
10 SGTC_146 3448-8650 6.41 53.1 µM 1.03E-8 NEO1-PIK1 46 7.30E-11
11 SGTC_1495 4469-0029 6.12 237.0 µM 4.22E-8 50 4.54E-10
12 SGTC_1286 0929-0070 5.97 131.0 µM 8.96E-8 5 1.20E-9
13 SGTC_2514 salinomycin 5.87 42.7 µM 1.44E-7 15 2.22E-9
14 SGTC_1846 st056244 5.86 14.8 µM 1.49E-7 5 2.33E-9
15 SGTC_391 gliotoxin 5.78 8.3 µM 2.18E-7 24 3.79E-9
16 SGTC_1665 st014639 5.76 11.4 µM 2.33E-7 NEO1 7 4.14E-9
17 SGTC_3148 9097893 5.73 49.5 µM 2.70E-7 15 4.99E-9
18 SGTC_232 clozapine 5.70 119.0 µM 3.10E-7 NEO1 36 5.98E-9
19 SGTC_2291 7917025 5.64 104.3 µM 4.07E-7 iron homeostasis 22 8.49E-9
20 SGTC_1027 k015-0025 5.58 8.7 µM 5.40E-7 3 1.23E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.273 1.77E-58 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.251 2.32E-49 YDL077C VAM6 hom Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
0.236 7.40E-44 YDR080W VPS41 hom Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
0.200 1.32E-31 YML001W YPT7 hom Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
0.174 2.54E-24 YJL024C APS3 hom Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
0.170 3.92E-23 YBR288C APM3 hom Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
0.165 7.88E-22 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.159 2.50E-20 YGR261C APL6 hom Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
0.158 3.55E-20 YDR372C VPS74 hom Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
0.150 2.96E-18 YPL195W APL5 hom Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function
0.145 2.69E-17 YDR455C_d YDR455C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
0.144 4.57E-17 YAL021C CCR4 hom Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening
0.138 1.03E-15 YGL124C MON1 hom Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate
0.137 1.35E-15 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.131 3.08E-14 YFL025C BST1 hom GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules