YOR108W / LEU9

Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR108W / LEU9

Click on Significant Values for Screen Details ID:SGTC_35|Compound:0133-0054|FD-Score:-3.10|P-value:9.96E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:3.70|P-value:7.96E-5 ID:SGTC_92|Compound:0063-0024|FD-Score:4.45|P-value:2.89E-6 ID:SGTC_174|Compound:3553-0389|FD-Score:4.25|P-value:7.58E-6 ID:SGTC_950|Compound:1165-0508|FD-Score:4.11|P-value:1.42E-5 ID:SGTC_978|Compound:1416-0512|FD-Score:4.43|P-value:3.30E-6 ID:SGTC_979|Compound:1426-2828|FD-Score:4.95|P-value:2.43E-7 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.70|P-value:8.24E-5 ID:SGTC_1286|Compound:0929-0070|FD-Score:4.66|P-value:1.04E-6 ID:SGTC_1325|Compound:1431-0015|FD-Score:3.92|P-value:3.17E-5 ID:SGTC_1436|Compound:4092-1007|FD-Score:3.04|P-value:9.41E-4 ID:SGTC_1547|Compound:harmine|FD-Score:4.19|P-value:1.01E-5 ID:SGTC_1833|Compound:st055984|FD-Score:3.69|P-value:8.48E-5 ID:SGTC_2002|Compound:4023503|FD-Score:-4.38|P-value:5.90E-6 ID:SGTC_2242|Compound:6630449|FD-Score:3.61|P-value:1.15E-4 ID:SGTC_2254|Compound:7938975|FD-Score:3.94|P-value:2.96E-5 ID:SGTC_2261|Compound:9010538|FD-Score:4.19|P-value:9.94E-6 ID:SGTC_2319|Compound:6033012|FD-Score:3.77|P-value:5.97E-5 ID:SGTC_2326|Compound:6164455|FD-Score:3.66|P-value:9.66E-5 ID:SGTC_2329|Compound:6138490|FD-Score:-3.36|P-value:4.01E-4 ID:SGTC_2363|Compound:9053361|FD-Score:-3.68|P-value:1.20E-4 ID:SGTC_2366|Compound:9070441|FD-Score:3.43|P-value:2.36E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:3.46|P-value:2.11E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:4.84|P-value:4.18E-7 ID:SGTC_2552|Compound:juarezic acid|FD-Score:3.48|P-value:1.92E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:3.41|P-value:2.54E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:-3.19|P-value:7.28E-4 ID:SGTC_2918|Compound:7977171|FD-Score:-3.62|P-value:1.52E-4 ID:SGTC_2936|Compound:9038235|FD-Score:3.04|P-value:9.34E-4 ID:SGTC_2990|Compound:9074105|FD-Score:3.27|P-value:4.18E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-3.69|P-value:1.16E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:-3.10|P-value:9.96E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:3.70|P-value:7.96E-5 ID:SGTC_92|Compound:0063-0024|FD-Score:4.45|P-value:2.89E-6 ID:SGTC_174|Compound:3553-0389|FD-Score:4.25|P-value:7.58E-6 ID:SGTC_950|Compound:1165-0508|FD-Score:4.11|P-value:1.42E-5 ID:SGTC_978|Compound:1416-0512|FD-Score:4.43|P-value:3.30E-6 ID:SGTC_979|Compound:1426-2828|FD-Score:4.95|P-value:2.43E-7 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.70|P-value:8.24E-5 ID:SGTC_1286|Compound:0929-0070|FD-Score:4.66|P-value:1.04E-6 ID:SGTC_1325|Compound:1431-0015|FD-Score:3.92|P-value:3.17E-5 ID:SGTC_1436|Compound:4092-1007|FD-Score:3.04|P-value:9.41E-4 ID:SGTC_1547|Compound:harmine|FD-Score:4.19|P-value:1.01E-5 ID:SGTC_1833|Compound:st055984|FD-Score:3.69|P-value:8.48E-5 ID:SGTC_2002|Compound:4023503|FD-Score:-4.38|P-value:5.90E-6 ID:SGTC_2242|Compound:6630449|FD-Score:3.61|P-value:1.15E-4 ID:SGTC_2254|Compound:7938975|FD-Score:3.94|P-value:2.96E-5 ID:SGTC_2261|Compound:9010538|FD-Score:4.19|P-value:9.94E-6 ID:SGTC_2319|Compound:6033012|FD-Score:3.77|P-value:5.97E-5 ID:SGTC_2326|Compound:6164455|FD-Score:3.66|P-value:9.66E-5 ID:SGTC_2329|Compound:6138490|FD-Score:-3.36|P-value:4.01E-4 ID:SGTC_2363|Compound:9053361|FD-Score:-3.68|P-value:1.20E-4 ID:SGTC_2366|Compound:9070441|FD-Score:3.43|P-value:2.36E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:3.46|P-value:2.11E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:4.84|P-value:4.18E-7 ID:SGTC_2552|Compound:juarezic acid|FD-Score:3.48|P-value:1.92E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:3.41|P-value:2.54E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:-3.19|P-value:7.28E-4 ID:SGTC_2918|Compound:7977171|FD-Score:-3.62|P-value:1.52E-4 ID:SGTC_2936|Compound:9038235|FD-Score:3.04|P-value:9.34E-4 ID:SGTC_2990|Compound:9074105|FD-Score:3.27|P-value:4.18E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-3.69|P-value:1.16E-4

Top fitness defect scores for YOR108W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_979 1426-2828 4.95 121.0 µM 2.43E-7 8 3.76E-7
2 SGTC_2500 5-alpha-androstan-3,17-dione 4.84 61.4 µM 4.18E-7 25 6.37E-7
3 SGTC_1286 0929-0070 4.66 131.0 µM 1.04E-6 12 1.55E-6
4 SGTC_92 0063-0024 4.45 660.9 µM 2.89E-6 4 4.20E-6
5 SGTC_978 1416-0512 4.43 87.4 µM 3.30E-6 redox potentiating 22 4.78E-6
6 SGTC_174 3553-0389 4.25 74.3 µM 7.58E-6 17 1.08E-5
7 SGTC_2261 9010538 4.19 184.7 µM 9.94E-6 14 1.40E-5
8 SGTC_1547 harmine 4.19 94.2 µM 1.01E-5 ergosterol biosynthesis 35 1.42E-5
9 SGTC_950 1165-0508 4.11 94.1 µM 1.42E-5 27 1.99E-5
10 SGTC_2254 7938975 3.94 95.2 µM 2.96E-5 56 4.05E-5
11 SGTC_1325 1431-0015 3.92 227.0 µM 3.17E-5 Golgi 20 4.34E-5
12 SGTC_2319 6033012 3.77 152.6 µM 5.97E-5 tubulin folding & SWR complex 36 8.03E-5
13 SGTC_40 0327-0103 3.70 19.8 µM 7.96E-5 39 1.06E-4
14 SGTC_1076 itraconazole 3.70 331.0 nM 8.24E-5 38 1.10E-4
15 SGTC_1833 st055984 3.69 71.0 µM 8.48E-5 22 1.13E-4
16 SGTC_2326 6164455 3.66 177.6 µM 9.66E-5 mitochondrial processes 33 1.28E-4
17 SGTC_2242 6630449 3.61 61.3 µM 1.15E-4 Golgi 53 1.52E-4
18 SGTC_2552 juarezic acid 3.48 87.0 µM 1.92E-4 TSC3-RPN4 56 2.51E-4
19 SGTC_2495 cafestol 3.46 54.5 µM 2.11E-4 Golgi 40 2.74E-4
20 SGTC_2366 9070441 3.43 60.6 µM 2.36E-4 53 3.06E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.114 4.13E-11 YPR054W SMK1 hom Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p
0.106 7.84E-10 YDR113C PDS1 het Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation
0.095 3.01E-8 YKL156W RPS27A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.090 1.75E-7 YDR170C SEC7 het Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles
0.083 1.61E-6 YMR221C_p YMR221C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy
0.082 2.01E-6 YKL198C PTK1 hom Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein
0.076 1.16E-5 YJL037W IRC18 hom Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci
0.076 1.18E-5 YNL096C RPS7B hom Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.074 1.88E-5 YLR329W REC102 hom Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination
0.073 2.31E-5 YGR051C_d YGR051C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene
0.072 2.62E-5 YDL041W_d YDL041W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C
0.072 2.79E-5 YNL039W BDP1 het Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs
0.070 4.71E-5 YPL190C NAB3 het RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing
0.070 4.78E-5 YPL223C GRE1 hom Hydrophilin essential in dessication-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway
0.070 5.73E-5 YLR260W LCB5 hom Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules