YOR127W / RGA1

GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR127W / RGA1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:8.17|P-value:4.47E-17 ID:SGTC_89|Compound:0302-0250|FD-Score:3.13|P-value:7.97E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:6.05|P-value:3.84E-10 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:-3.17|P-value:5.18E-4 ID:SGTC_274|Compound:sirtinol|FD-Score:-3.10|P-value:6.69E-4 ID:SGTC_332|Compound:4112-0506|FD-Score:-3.57|P-value:1.13E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:3.12|P-value:8.14E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:-5.12|P-value:6.78E-8 ID:SGTC_366|Compound:0181-0206|FD-Score:4.06|P-value:1.99E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.15|P-value:5.56E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:3.77|P-value:6.66E-5 ID:SGTC_967|Compound:1306-0166|FD-Score:5.15|P-value:8.46E-8 ID:SGTC_1060|Compound:3448-7350|FD-Score:5.93|P-value:8.60E-10 ID:SGTC_1700|Compound:st030840|FD-Score:3.11|P-value:8.45E-4 ID:SGTC_1712|Compound:st032288|FD-Score:3.78|P-value:6.45E-5 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.18|P-value:6.48E-4 ID:SGTC_1979|Compound:st071809|FD-Score:4.50|P-value:2.50E-6 ID:SGTC_2017|Compound:5132927|FD-Score:3.58|P-value:1.46E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.56|P-value:1.61E-4 ID:SGTC_2257|Compound:7960413|FD-Score:3.28|P-value:4.62E-4 ID:SGTC_2351|Compound:9026858|FD-Score:3.56|P-value:1.60E-4 ID:SGTC_2369|Compound:9071472|FD-Score:3.08|P-value:9.43E-4 ID:SGTC_2402|Compound:5553605|FD-Score:3.12|P-value:8.21E-4 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:-3.26|P-value:3.72E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.84|P-value:5.05E-5 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.39|P-value:2.32E-4 ID:SGTC_2812|Compound:7970281|FD-Score:4.04|P-value:2.11E-5 ID:SGTC_2815|Compound:7968870|FD-Score:3.52|P-value:1.85E-4 ID:SGTC_2854|Compound:9024559|FD-Score:3.96|P-value:3.07E-5 ID:SGTC_2870|Compound:9038528|FD-Score:3.18|P-value:6.65E-4 ID:SGTC_2890|Compound:9046729|FD-Score:4.57|P-value:1.77E-6 ID:SGTC_2933|Compound:9026794|FD-Score:3.15|P-value:7.45E-4 ID:SGTC_3311|Compound:9131146|FD-Score:3.12|P-value:8.22E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:8.17|P-value:4.47E-17 ID:SGTC_89|Compound:0302-0250|FD-Score:3.13|P-value:7.97E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:6.05|P-value:3.84E-10 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:-3.17|P-value:5.18E-4 ID:SGTC_274|Compound:sirtinol|FD-Score:-3.10|P-value:6.69E-4 ID:SGTC_332|Compound:4112-0506|FD-Score:-3.57|P-value:1.13E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:3.12|P-value:8.14E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:-5.12|P-value:6.78E-8 ID:SGTC_366|Compound:0181-0206|FD-Score:4.06|P-value:1.99E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.15|P-value:5.56E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:3.77|P-value:6.66E-5 ID:SGTC_967|Compound:1306-0166|FD-Score:5.15|P-value:8.46E-8 ID:SGTC_1060|Compound:3448-7350|FD-Score:5.93|P-value:8.60E-10 ID:SGTC_1700|Compound:st030840|FD-Score:3.11|P-value:8.45E-4 ID:SGTC_1712|Compound:st032288|FD-Score:3.78|P-value:6.45E-5 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.18|P-value:6.48E-4 ID:SGTC_1979|Compound:st071809|FD-Score:4.50|P-value:2.50E-6 ID:SGTC_2017|Compound:5132927|FD-Score:3.58|P-value:1.46E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.56|P-value:1.61E-4 ID:SGTC_2257|Compound:7960413|FD-Score:3.28|P-value:4.62E-4 ID:SGTC_2351|Compound:9026858|FD-Score:3.56|P-value:1.60E-4 ID:SGTC_2369|Compound:9071472|FD-Score:3.08|P-value:9.43E-4 ID:SGTC_2402|Compound:5553605|FD-Score:3.12|P-value:8.21E-4 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:-3.26|P-value:3.72E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.84|P-value:5.05E-5 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.39|P-value:2.32E-4 ID:SGTC_2812|Compound:7970281|FD-Score:4.04|P-value:2.11E-5 ID:SGTC_2815|Compound:7968870|FD-Score:3.52|P-value:1.85E-4 ID:SGTC_2854|Compound:9024559|FD-Score:3.96|P-value:3.07E-5 ID:SGTC_2870|Compound:9038528|FD-Score:3.18|P-value:6.65E-4 ID:SGTC_2890|Compound:9046729|FD-Score:4.57|P-value:1.77E-6 ID:SGTC_2933|Compound:9026794|FD-Score:3.15|P-value:7.45E-4 ID:SGTC_3311|Compound:9131146|FD-Score:3.12|P-value:8.22E-4

Top fitness defect scores for YOR127W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 8.17 9.0 µM 4.47E-17 cell wall 115 1.49E-16
2 SGTC_138 0850-0259 6.05 40.9 µM 3.84E-10 cell wall 104 7.02E-10
3 SGTC_1060 3448-7350 5.93 82.0 µM 8.60E-10 Golgi 12 1.53E-9
4 SGTC_967 1306-0166 5.15 352.0 µM 8.46E-8 10 1.28E-7
5 SGTC_2890 9046729 4.57 58.4 µM 1.77E-6 Golgi 15 2.40E-6
6 SGTC_1979 st071809 4.50 45.3 µM 2.50E-6 RPP1 & pyrimidine depletion 5 3.35E-6
7 SGTC_366 0181-0206 4.06 4.3 µM 1.99E-5 Golgi 36 2.48E-5
8 SGTC_2812 7970281 4.04 58.4 µM 2.11E-5 27 2.64E-5
9 SGTC_2854 9024559 3.96 71.4 µM 3.07E-5 20 3.79E-5
10 SGTC_2584 androsterone 3.84 48.7 µM 5.05E-5 cell wall 140 6.13E-5
11 SGTC_1712 st032288 3.78 53.4 µM 6.45E-5 calcium & mitochondrial duress 200 7.76E-5
12 SGTC_863 0466-0191 3.77 129.0 µM 6.66E-5 cell wall 76 8.01E-5
13 SGTC_2017 5132927 3.58 60.6 µM 1.46E-4 39 1.71E-4
14 SGTC_2351 9026858 3.56 72.3 µM 1.60E-4 RNA processing & uracil transport 36 1.87E-4
15 SGTC_2021 4019055 3.56 173.0 µM 1.61E-4 endomembrane recycling 148 1.88E-4
16 SGTC_2815 7968870 3.52 71.4 µM 1.85E-4 39 2.15E-4
17 SGTC_2257 7960413 3.28 200.0 µM 4.62E-4 NEO1-PIK1 87 5.22E-4
18 SGTC_1873 rapamycin 3.18 2.0 nM 6.48E-4 109 7.25E-4
19 SGTC_2870 9038528 3.18 13.0 µM 6.65E-4 45 7.44E-4
20 SGTC_2933 9026794 3.15 32.5 µM 7.45E-4 64 8.30E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.341 3.03E-92 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.338 2.16E-90 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.317 5.88E-79 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.313 3.38E-77 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.306 1.61E-73 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.302 8.88E-72 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.297 2.49E-69 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.297 3.82E-69 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.289 2.22E-65 YJL080C SCP160 hom Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
0.283 4.49E-63 YPL115C BEM3 hom Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.282 3.70E-62 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.268 2.80E-56 YPL269W KAR9 hom Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
0.268 3.04E-56 YKL033W-A_p YKL033W-A_p hom Putative protein of unknown function; similar to uncharacterized proteins from other fungi
0.263 3.59E-54 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.260 6.45E-53 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication