YOR133W / EFT1

Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR133W / EFT1

Click on Significant Values for Screen Details ID:SGTC_3|Compound:0833-0587|FD-Score:3.55|P-value:2.60E-4 ID:SGTC_4|Compound:0960-0234|FD-Score:3.57|P-value:2.39E-4 ID:SGTC_9|Compound:0092-0002|FD-Score:4.57|P-value:3.94E-6 ID:SGTC_17|Compound:0960-0276|FD-Score:4.47|P-value:6.13E-6 ID:SGTC_49|Compound:0717-0965|FD-Score:3.18|P-value:9.24E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:5.36|P-value:7.55E-8 ID:SGTC_126|Compound:0417-1624|FD-Score:3.32|P-value:5.90E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:4.71|P-value:2.01E-6 ID:SGTC_145|Compound:3737-0113|FD-Score:6.64|P-value:3.94E-11 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.93|P-value:6.10E-5 ID:SGTC_257|Compound:daunorubicin|FD-Score:7.57|P-value:6.06E-14 ID:SGTC_264|Compound:promethazine|FD-Score:16.90|P-value:3.50E-62 ID:SGTC_317|Compound:4495-0002|FD-Score:3.43|P-value:3.92E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:5.11|P-value:2.82E-7 ID:SGTC_407|Compound:eburnamonine|FD-Score:3.35|P-value:5.33E-4 ID:SGTC_470|Compound:aa-861|FD-Score:3.82|P-value:9.26E-5 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:5.87|P-value:4.52E-9 ID:SGTC_660|Compound:4031-1742|FD-Score:3.70|P-value:1.48E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:4.27|P-value:1.47E-5 ID:SGTC_872|Compound:0588-0139|FD-Score:3.72|P-value:1.36E-4 ID:SGTC_880|Compound:0833-0487|FD-Score:3.28|P-value:6.78E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:3.52|P-value:2.86E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:4.18|P-value:2.15E-5 ID:SGTC_1015|Compound:4130-4525|FD-Score:4.42|P-value:7.45E-6 ID:SGTC_1020|Compound:4335-0961|FD-Score:3.28|P-value:6.80E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:4.58|P-value:3.69E-6 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:3.22|P-value:8.14E-4 ID:SGTC_1277|Compound:0873-0036|FD-Score:3.52|P-value:2.90E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:4.70|P-value:2.15E-6 ID:SGTC_1299|Compound:1079-1439|FD-Score:3.54|P-value:2.66E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:3.32|P-value:5.91E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:3.92|P-value:6.28E-5 ID:SGTC_1384|Compound:3089-0840|FD-Score:3.27|P-value:7.00E-4 ID:SGTC_1461|Compound:k072-0203|FD-Score:3.32|P-value:5.84E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:4.54|P-value:4.50E-6 ID:SGTC_1515|Compound:3460-0031|FD-Score:4.98|P-value:5.36E-7 ID:SGTC_1519|Compound:esterone|FD-Score:5.98|P-value:2.34E-9 ID:SGTC_1529|Compound:apigenin|FD-Score:4.35|P-value:1.02E-5 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:3.32|P-value:5.77E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:3.60|P-value:2.19E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:3.91|P-value:6.51E-5 ID:SGTC_1612|Compound:st002233|FD-Score:3.16|P-value:9.90E-4 ID:SGTC_1677|Compound:st019204|FD-Score:-4.71|P-value:1.86E-6 ID:SGTC_1678|Compound:st019265|FD-Score:-3.55|P-value:2.43E-4 ID:SGTC_1683|Compound:st010134|FD-Score:-5.14|P-value:2.22E-7 ID:SGTC_1701|Compound:st028638|FD-Score:4.83|P-value:1.11E-6 ID:SGTC_1711|Compound:st032263|FD-Score:3.35|P-value:5.31E-4 ID:SGTC_1914|Compound:st062367|FD-Score:3.19|P-value:9.03E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:4.12|P-value:2.74E-5 ID:SGTC_2031|Compound:5180455|FD-Score:3.21|P-value:8.42E-4 ID:SGTC_2069|Compound:5228469|FD-Score:3.83|P-value:8.98E-5 ID:SGTC_2320|Compound:7373003|FD-Score:-3.22|P-value:7.62E-4 ID:SGTC_2397|Compound:5161689|FD-Score:3.33|P-value:5.64E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.11|P-value:2.68E-5 ID:SGTC_2564|Compound:betulinic acid|FD-Score:4.80|P-value:1.34E-6 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:7.54|P-value:7.58E-14 ID:SGTC_2576|Compound:guaiol|FD-Score:3.85|P-value:8.22E-5 ID:SGTC_2589|Compound:lobaric acid|FD-Score:4.86|P-value:9.68E-7 ID:SGTC_2706|Compound:st078239|FD-Score:-4.10|P-value:2.77E-5 ID:SGTC_2812|Compound:7970281|FD-Score:3.38|P-value:4.78E-4 ID:SGTC_3040|Compound:9091894|FD-Score:6.49|P-value:1.02E-10 ID:SGTC_3131|Compound:9127911|FD-Score:-4.17|P-value:2.10E-5 ID:SGTC_3145|Compound:9085516|FD-Score:3.60|P-value:2.14E-4 ID:SGTC_3153|Compound:9100258|FD-Score:3.58|P-value:2.31E-4 ID:SGTC_3283|Compound:9109332|FD-Score:4.04|P-value:3.90E-5 ID:SGTC_3294|Compound:9122953|FD-Score:3.55|P-value:2.58E-4 ID:SGTC_3310|Compound:9130229|FD-Score:4.74|P-value:1.79E-6 ID:SGTC_3346|Compound:9158254|FD-Score:3.25|P-value:7.36E-4 ID:SGTC_3|Compound:0833-0587|FD-Score:3.55|P-value:2.60E-4 ID:SGTC_4|Compound:0960-0234|FD-Score:3.57|P-value:2.39E-4 ID:SGTC_9|Compound:0092-0002|FD-Score:4.57|P-value:3.94E-6 ID:SGTC_17|Compound:0960-0276|FD-Score:4.47|P-value:6.13E-6 ID:SGTC_49|Compound:0717-0965|FD-Score:3.18|P-value:9.24E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:5.36|P-value:7.55E-8 ID:SGTC_126|Compound:0417-1624|FD-Score:3.32|P-value:5.90E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:4.71|P-value:2.01E-6 ID:SGTC_145|Compound:3737-0113|FD-Score:6.64|P-value:3.94E-11 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.93|P-value:6.10E-5 ID:SGTC_257|Compound:daunorubicin|FD-Score:7.57|P-value:6.06E-14 ID:SGTC_264|Compound:promethazine|FD-Score:16.90|P-value:3.50E-62 ID:SGTC_317|Compound:4495-0002|FD-Score:3.43|P-value:3.92E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:5.11|P-value:2.82E-7 ID:SGTC_407|Compound:eburnamonine|FD-Score:3.35|P-value:5.33E-4 ID:SGTC_470|Compound:aa-861|FD-Score:3.82|P-value:9.26E-5 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:5.87|P-value:4.52E-9 ID:SGTC_660|Compound:4031-1742|FD-Score:3.70|P-value:1.48E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:4.27|P-value:1.47E-5 ID:SGTC_872|Compound:0588-0139|FD-Score:3.72|P-value:1.36E-4 ID:SGTC_880|Compound:0833-0487|FD-Score:3.28|P-value:6.78E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:3.52|P-value:2.86E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:4.18|P-value:2.15E-5 ID:SGTC_1015|Compound:4130-4525|FD-Score:4.42|P-value:7.45E-6 ID:SGTC_1020|Compound:4335-0961|FD-Score:3.28|P-value:6.80E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:4.58|P-value:3.69E-6 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:3.22|P-value:8.14E-4 ID:SGTC_1277|Compound:0873-0036|FD-Score:3.52|P-value:2.90E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:4.70|P-value:2.15E-6 ID:SGTC_1299|Compound:1079-1439|FD-Score:3.54|P-value:2.66E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:3.32|P-value:5.91E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:3.92|P-value:6.28E-5 ID:SGTC_1384|Compound:3089-0840|FD-Score:3.27|P-value:7.00E-4 ID:SGTC_1461|Compound:k072-0203|FD-Score:3.32|P-value:5.84E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:4.54|P-value:4.50E-6 ID:SGTC_1515|Compound:3460-0031|FD-Score:4.98|P-value:5.36E-7 ID:SGTC_1519|Compound:esterone|FD-Score:5.98|P-value:2.34E-9 ID:SGTC_1529|Compound:apigenin|FD-Score:4.35|P-value:1.02E-5 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:3.32|P-value:5.77E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:3.60|P-value:2.19E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:3.91|P-value:6.51E-5 ID:SGTC_1612|Compound:st002233|FD-Score:3.16|P-value:9.90E-4 ID:SGTC_1677|Compound:st019204|FD-Score:-4.71|P-value:1.86E-6 ID:SGTC_1678|Compound:st019265|FD-Score:-3.55|P-value:2.43E-4 ID:SGTC_1683|Compound:st010134|FD-Score:-5.14|P-value:2.22E-7 ID:SGTC_1701|Compound:st028638|FD-Score:4.83|P-value:1.11E-6 ID:SGTC_1711|Compound:st032263|FD-Score:3.35|P-value:5.31E-4 ID:SGTC_1914|Compound:st062367|FD-Score:3.19|P-value:9.03E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:4.12|P-value:2.74E-5 ID:SGTC_2031|Compound:5180455|FD-Score:3.21|P-value:8.42E-4 ID:SGTC_2069|Compound:5228469|FD-Score:3.83|P-value:8.98E-5 ID:SGTC_2320|Compound:7373003|FD-Score:-3.22|P-value:7.62E-4 ID:SGTC_2397|Compound:5161689|FD-Score:3.33|P-value:5.64E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.11|P-value:2.68E-5 ID:SGTC_2564|Compound:betulinic acid|FD-Score:4.80|P-value:1.34E-6 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:7.54|P-value:7.58E-14 ID:SGTC_2576|Compound:guaiol|FD-Score:3.85|P-value:8.22E-5 ID:SGTC_2589|Compound:lobaric acid|FD-Score:4.86|P-value:9.68E-7 ID:SGTC_2706|Compound:st078239|FD-Score:-4.10|P-value:2.77E-5 ID:SGTC_2812|Compound:7970281|FD-Score:3.38|P-value:4.78E-4 ID:SGTC_3040|Compound:9091894|FD-Score:6.49|P-value:1.02E-10 ID:SGTC_3131|Compound:9127911|FD-Score:-4.17|P-value:2.10E-5 ID:SGTC_3145|Compound:9085516|FD-Score:3.60|P-value:2.14E-4 ID:SGTC_3153|Compound:9100258|FD-Score:3.58|P-value:2.31E-4 ID:SGTC_3283|Compound:9109332|FD-Score:4.04|P-value:3.90E-5 ID:SGTC_3294|Compound:9122953|FD-Score:3.55|P-value:2.58E-4 ID:SGTC_3310|Compound:9130229|FD-Score:4.74|P-value:1.79E-6 ID:SGTC_3346|Compound:9158254|FD-Score:3.25|P-value:7.36E-4

Top fitness defect scores for YOR133W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_264 promethazine 16.90 12.5 mM 3.50E-62 DNA intercalators 5 1.26E-64
2 SGTC_257 daunorubicin 7.57 18.5 µM 6.06E-14 anthracycline transcription coupled DNA repair 8 1.86E-14
3 SGTC_2573 2',4'-dihydroxychalcone 7.54 15.0 µM 7.58E-14 8 2.35E-14
4 SGTC_145 3737-0113 6.64 38.4 µM 3.94E-11 DNA intercalators 18 1.57E-11
5 SGTC_3040 9091894 6.49 49.5 µM 1.02E-10 DNA intercalators 6 4.22E-11
6 SGTC_1519 esterone 5.98 74.0 µM 2.34E-9 DNA intercalators 5 1.10E-9
7 SGTC_471 1-acyl-paf 5.87 4.0 µM 4.52E-9 DNA intercalators 7 2.18E-9
8 SGTC_112 phenothiazine 5.36 287.2 µM 7.55E-8 unfolded protein response 6 4.07E-8
9 SGTC_370 0180-0345 5.11 38.7 µM 2.82E-7 DNA intercalators 4 1.60E-7
10 SGTC_1515 3460-0031 4.98 42.1 µM 5.36E-7 DNA intercalators 7 3.13E-7
11 SGTC_2589 lobaric acid 4.86 100.0 µM 9.68E-7 14 5.79E-7
12 SGTC_1701 st028638 4.83 40.4 µM 1.11E-6 3 6.68E-7
13 SGTC_2564 betulinic acid 4.80 100.0 µM 1.34E-6 15 8.13E-7
14 SGTC_3310 9130229 4.74 44.1 µM 1.79E-6 Golgi 10 1.09E-6
15 SGTC_144 4161-2749 4.71 112.4 µM 2.01E-6 DNA intercalators 14 1.24E-6
16 SGTC_1290 0986-0249 4.70 10.2 µM 2.15E-6 DNA intercalators 11 1.33E-6
17 SGTC_1060 3448-7350 4.58 82.0 µM 3.69E-6 Golgi 24 2.33E-6
18 SGTC_9 0092-0002 4.57 10.4 µM 3.94E-6 16 2.49E-6
19 SGTC_1468 k284-1086 4.54 134.0 µM 4.50E-6 DNA intercalators 19 2.86E-6
20 SGTC_17 0960-0276 4.47 49.8 µM 6.13E-6 17 3.94E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.509 1.43E-220 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.507 5.72E-219 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.506 3.34E-217 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.474 6.72E-188 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.468 6.88E-182 YDL223C HBT1 hom Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.440 2.43E-158 YJL025W RRN7 het Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
0.407 6.06E-134 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.366 9.02E-107 YGL200C EMP24 hom Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
0.366 1.06E-106 YGR065C VHT1 het High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
0.343 3.04E-93 YPL157W TGS1 hom Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
0.342 3.62E-92 YGL041C_d YGL041C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.336 1.96E-89 YEL032W MCM3 het Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
0.329 2.71E-85 YMR262W_p YMR262W_p hom Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene
0.267 9.24E-56 YLR133W CKI1 hom Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication
0.267 1.08E-55 YHR172W SPC97 het Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque