YOR136W / IDH2

Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR136W / IDH2

Click on Significant Values for Screen Details ID:SGTC_38|Compound:0929-0051|FD-Score:3.50|P-value:8.52E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:8.11|P-value:1.62E-13 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.44|P-value:9.01E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:5.22|P-value:1.40E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:6.33|P-value:6.75E-9 ID:SGTC_253|Compound:dyclonine|FD-Score:7.78|P-value:1.34E-12 ID:SGTC_359|Compound:0081-0058|FD-Score:11.30|P-value:1.87E-24 ID:SGTC_379|Compound:3970-0972|FD-Score:4.01|P-value:1.65E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:3.54|P-value:7.55E-4 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:3.60|P-value:6.27E-4 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:4.53|P-value:2.44E-5 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.71|P-value:4.44E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:7.59|P-value:4.44E-12 ID:SGTC_997|Compound:3996-0078|FD-Score:6.48|P-value:3.04E-9 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.87|P-value:2.65E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.51|P-value:8.41E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:7.33|P-value:2.34E-11 ID:SGTC_1080|Compound:nisoldipine|FD-Score:5.18|P-value:1.65E-6 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:4.16|P-value:9.59E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:4.45|P-value:3.35E-5 ID:SGTC_1167|Compound:k781-0610|FD-Score:3.46|P-value:9.68E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:5.17|P-value:1.76E-6 ID:SGTC_1260|Compound:0700-1617|FD-Score:4.51|P-value:2.67E-5 ID:SGTC_1334|Compound:1486-0610|FD-Score:6.28|P-value:8.40E-9 ID:SGTC_1397|Compound:3532-0190|FD-Score:4.33|P-value:5.16E-5 ID:SGTC_1418|Compound:3966-3099|FD-Score:3.73|P-value:4.22E-4 ID:SGTC_1440|Compound:4100-1550|FD-Score:4.47|P-value:3.09E-5 ID:SGTC_1483|Compound:3453-0561|FD-Score:5.18|P-value:1.67E-6 ID:SGTC_1522|Compound:st012842|FD-Score:5.63|P-value:2.20E-7 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.51|P-value:8.43E-4 ID:SGTC_1716|Compound:st033144|FD-Score:-3.90|P-value:2.08E-4 ID:SGTC_1843|Compound:st055809|FD-Score:4.76|P-value:9.99E-6 ID:SGTC_1860|Compound:st056228|FD-Score:3.95|P-value:2.02E-4 ID:SGTC_1901|Compound:st060216|FD-Score:5.57|P-value:2.83E-7 ID:SGTC_1983|Compound:st071813|FD-Score:3.73|P-value:4.23E-4 ID:SGTC_2006|Compound:4011487|FD-Score:-3.52|P-value:7.14E-4 ID:SGTC_2053|Compound:5265679|FD-Score:3.55|P-value:7.45E-4 ID:SGTC_2060|Compound:5238662|FD-Score:3.60|P-value:6.24E-4 ID:SGTC_2297|Compound:7971671|FD-Score:3.86|P-value:2.70E-4 ID:SGTC_2348|Compound:9022543|FD-Score:5.42|P-value:5.62E-7 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:5.97|P-value:4.12E-8 ID:SGTC_2523|Compound:spermine|FD-Score:3.66|P-value:5.15E-4 ID:SGTC_2591|Compound:skatole|FD-Score:4.64|P-value:1.60E-5 ID:SGTC_2671|Compound:danthron|FD-Score:3.60|P-value:6.36E-4 ID:SGTC_3234|Compound:9132280|FD-Score:3.64|P-value:5.49E-4 ID:SGTC_3274|Compound:9138298|FD-Score:5.15|P-value:1.92E-6 ID:SGTC_3319|Compound:9138450|FD-Score:4.30|P-value:5.72E-5 ID:SGTC_38|Compound:0929-0051|FD-Score:3.50|P-value:8.52E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:8.11|P-value:1.62E-13 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.44|P-value:9.01E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:5.22|P-value:1.40E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:6.33|P-value:6.75E-9 ID:SGTC_253|Compound:dyclonine|FD-Score:7.78|P-value:1.34E-12 ID:SGTC_359|Compound:0081-0058|FD-Score:11.30|P-value:1.87E-24 ID:SGTC_379|Compound:3970-0972|FD-Score:4.01|P-value:1.65E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:3.54|P-value:7.55E-4 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:3.60|P-value:6.27E-4 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:4.53|P-value:2.44E-5 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.71|P-value:4.44E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:7.59|P-value:4.44E-12 ID:SGTC_997|Compound:3996-0078|FD-Score:6.48|P-value:3.04E-9 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.87|P-value:2.65E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.51|P-value:8.41E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:7.33|P-value:2.34E-11 ID:SGTC_1080|Compound:nisoldipine|FD-Score:5.18|P-value:1.65E-6 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:4.16|P-value:9.59E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:4.45|P-value:3.35E-5 ID:SGTC_1167|Compound:k781-0610|FD-Score:3.46|P-value:9.68E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:5.17|P-value:1.76E-6 ID:SGTC_1260|Compound:0700-1617|FD-Score:4.51|P-value:2.67E-5 ID:SGTC_1334|Compound:1486-0610|FD-Score:6.28|P-value:8.40E-9 ID:SGTC_1397|Compound:3532-0190|FD-Score:4.33|P-value:5.16E-5 ID:SGTC_1418|Compound:3966-3099|FD-Score:3.73|P-value:4.22E-4 ID:SGTC_1440|Compound:4100-1550|FD-Score:4.47|P-value:3.09E-5 ID:SGTC_1483|Compound:3453-0561|FD-Score:5.18|P-value:1.67E-6 ID:SGTC_1522|Compound:st012842|FD-Score:5.63|P-value:2.20E-7 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.51|P-value:8.43E-4 ID:SGTC_1716|Compound:st033144|FD-Score:-3.90|P-value:2.08E-4 ID:SGTC_1843|Compound:st055809|FD-Score:4.76|P-value:9.99E-6 ID:SGTC_1860|Compound:st056228|FD-Score:3.95|P-value:2.02E-4 ID:SGTC_1901|Compound:st060216|FD-Score:5.57|P-value:2.83E-7 ID:SGTC_1983|Compound:st071813|FD-Score:3.73|P-value:4.23E-4 ID:SGTC_2006|Compound:4011487|FD-Score:-3.52|P-value:7.14E-4 ID:SGTC_2053|Compound:5265679|FD-Score:3.55|P-value:7.45E-4 ID:SGTC_2060|Compound:5238662|FD-Score:3.60|P-value:6.24E-4 ID:SGTC_2297|Compound:7971671|FD-Score:3.86|P-value:2.70E-4 ID:SGTC_2348|Compound:9022543|FD-Score:5.42|P-value:5.62E-7 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:5.97|P-value:4.12E-8 ID:SGTC_2523|Compound:spermine|FD-Score:3.66|P-value:5.15E-4 ID:SGTC_2591|Compound:skatole|FD-Score:4.64|P-value:1.60E-5 ID:SGTC_2671|Compound:danthron|FD-Score:3.60|P-value:6.36E-4 ID:SGTC_3234|Compound:9132280|FD-Score:3.64|P-value:5.49E-4 ID:SGTC_3274|Compound:9138298|FD-Score:5.15|P-value:1.92E-6 ID:SGTC_3319|Compound:9138450|FD-Score:4.30|P-value:5.72E-5

Top fitness defect scores for YOR136W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_359 0081-0058 11.30 15.9 µM 1.87E-24 60S ribosome export 2 8.84E-30
2 SGTC_44 0929-0055 8.11 61.2 µM 1.62E-13 3 2.62E-16
3 SGTC_253 dyclonine 7.78 31.2 µM 1.34E-12 amide catabolism 6 3.51E-15
4 SGTC_496 calpeptin 7.59 138.0 µM 4.44E-12 2 1.54E-14
5 SGTC_1061 3448-9325 7.33 110.0 µM 2.34E-11 1 1.19E-13
6 SGTC_997 3996-0078 6.48 137.0 µM 3.04E-9 2 4.73E-11
7 SGTC_117 lucigenin 6.33 22.1 µM 6.75E-9 redox potentiating 36 1.26E-10
8 SGTC_1334 1486-0610 6.28 236.0 µM 8.40E-9 60S ribosome export 2 1.65E-10
9 SGTC_2509 methyl 7-deshydroxypyrogallin-4-carboxylate 5.97 54.6 µM 4.12E-8 RPP1 & pyrimidine depletion 8 1.17E-9
10 SGTC_1522 st012842 5.63 45.6 µM 2.20E-7 RNA pol III & RNase P/MRP 8 9.14E-9
11 SGTC_1901 st060216 5.57 58.6 µM 2.83E-7 6 1.25E-8
12 SGTC_2348 9022543 5.42 33.4 µM 5.62E-7 mitochondrial processes 10 2.90E-8
13 SGTC_66 0109-0187 5.22 42.4 µM 1.40E-6 19 8.90E-8
14 SGTC_1080 nisoldipine 5.18 24.4 µM 1.65E-6 TSC3-RPN4 8 1.09E-7
15 SGTC_1483 3453-0561 5.18 55.9 µM 1.67E-6 6 1.11E-7
16 SGTC_1204 4049-0315 5.17 18.3 µM 1.76E-6 5 1.18E-7
17 SGTC_3274 9138298 5.15 49.5 µM 1.92E-6 5 1.31E-7
18 SGTC_1843 st055809 4.76 45.5 µM 9.99E-6 7 9.91E-7
19 SGTC_2591 skatole 4.64 100.0 µM 1.60E-5 20 1.76E-6
20 SGTC_454 lyso-paf C-16 4.53 6.7 µM 2.44E-5 11 2.96E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.131 2.27E-14 YDL140C RPO21 het RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
0.120 3.68E-12 YOL129W VPS68 hom Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria
0.104 1.65E-9 YJL082W IML2 hom Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication
0.103 2.12E-9 YOR329C SCD5 het Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
0.100 5.99E-9 YOR291W YPK9 hom Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
0.098 1.37E-8 YJR017C ESS1 het Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3
0.091 1.10E-7 YDL163W_d YDL163W_d het Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase
0.090 1.57E-7 YHL032C GUT1 hom Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p
0.088 3.03E-7 YMR229C RRP5 het RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress
0.085 9.29E-7 YJR115W_p YJR115W_p hom Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication
0.082 2.00E-6 YML030W RCF1 hom Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast
0.080 3.31E-6 YKL078W DHR2 het Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
0.080 3.50E-6 YOR373W NUD1 het Component of the spindle pole body outer plaque; acts through the mitotic exit network to specify asymmetric spindle pole body inheritance
0.080 3.82E-6 YOR149C SMP3 het Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure
0.080 3.88E-6 YBR215W HPC2 hom Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes