YOR148C / SPP2

Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOR148C / SPP2

Click on Significant Values for Screen Details ID:SGTC_136|Compound:0097-0015|FD-Score:-3.27|P-value:3.67E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:3.03|P-value:7.10E-4 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.28|P-value:2.82E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-3.02|P-value:9.14E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-5.26|P-value:2.51E-8 ID:SGTC_903|Compound:1134-0042|FD-Score:3.65|P-value:6.41E-5 ID:SGTC_1007|Compound:4101-0031|FD-Score:3.11|P-value:5.46E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:3.17|P-value:4.29E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:3.09|P-value:5.87E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:2.95|P-value:9.65E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.02|P-value:7.50E-4 ID:SGTC_1437|Compound:4092-1038|FD-Score:3.19|P-value:4.03E-4 ID:SGTC_1646|Compound:st011709|FD-Score:3.84|P-value:2.78E-5 ID:SGTC_1683|Compound:st010134|FD-Score:2.99|P-value:8.41E-4 ID:SGTC_1744|Compound:st037937|FD-Score:-3.50|P-value:1.47E-4 ID:SGTC_1780|Compound:st038448|FD-Score:3.48|P-value:1.27E-4 ID:SGTC_1939|Compound:st074455|FD-Score:3.11|P-value:5.42E-4 ID:SGTC_1963|Compound:st076565|FD-Score:3.30|P-value:2.60E-4 ID:SGTC_1984|Compound:st072354|FD-Score:-3.35|P-value:2.74E-4 ID:SGTC_1989|Compound:st071868|FD-Score:3.92|P-value:1.97E-5 ID:SGTC_2000|Compound:st075254|FD-Score:-3.14|P-value:5.99E-4 ID:SGTC_2036|Compound:5160441|FD-Score:4.17|P-value:6.14E-6 ID:SGTC_2147|Compound:5545932|FD-Score:3.96|P-value:1.65E-5 ID:SGTC_2218|Compound:6965428|FD-Score:4.87|P-value:1.66E-7 ID:SGTC_2399|Compound:5884133|FD-Score:3.24|P-value:3.25E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:5.11|P-value:4.24E-8 ID:SGTC_2448|Compound:5185112|FD-Score:5.83|P-value:5.32E-10 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:3.58|P-value:8.52E-5 ID:SGTC_2720|Compound:fluspirilene|FD-Score:3.27|P-value:2.94E-4 ID:SGTC_2726|Compound:cimetidine|FD-Score:-3.54|P-value:1.26E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:-3.60|P-value:9.76E-5 ID:SGTC_2835|Compound:9006588|FD-Score:3.82|P-value:3.05E-5 ID:SGTC_2850|Compound:9015429|FD-Score:3.07|P-value:6.11E-4 ID:SGTC_2892|Compound:9047358|FD-Score:4.24|P-value:4.32E-6 ID:SGTC_2894|Compound:9048625|FD-Score:-4.97|P-value:1.31E-7 ID:SGTC_2930|Compound:9013230|FD-Score:3.15|P-value:4.65E-4 ID:SGTC_2937|Compound:9044753|FD-Score:3.98|P-value:1.50E-5 ID:SGTC_2946|Compound:9065502|FD-Score:-3.12|P-value:6.38E-4 ID:SGTC_2950|Compound:9072895|FD-Score:3.21|P-value:3.63E-4 ID:SGTC_3114|Compound:9122766|FD-Score:-3.28|P-value:3.54E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-3.69|P-value:6.83E-5 ID:SGTC_3158|Compound:9100007|FD-Score:4.49|P-value:1.24E-6 ID:SGTC_3159|Compound:9100096|FD-Score:3.23|P-value:3.44E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:-3.27|P-value:3.67E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:3.03|P-value:7.10E-4 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.28|P-value:2.82E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-3.02|P-value:9.14E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-5.26|P-value:2.51E-8 ID:SGTC_903|Compound:1134-0042|FD-Score:3.65|P-value:6.41E-5 ID:SGTC_1007|Compound:4101-0031|FD-Score:3.11|P-value:5.46E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:3.17|P-value:4.29E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:3.09|P-value:5.87E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:2.95|P-value:9.65E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.02|P-value:7.50E-4 ID:SGTC_1437|Compound:4092-1038|FD-Score:3.19|P-value:4.03E-4 ID:SGTC_1646|Compound:st011709|FD-Score:3.84|P-value:2.78E-5 ID:SGTC_1683|Compound:st010134|FD-Score:2.99|P-value:8.41E-4 ID:SGTC_1744|Compound:st037937|FD-Score:-3.50|P-value:1.47E-4 ID:SGTC_1780|Compound:st038448|FD-Score:3.48|P-value:1.27E-4 ID:SGTC_1939|Compound:st074455|FD-Score:3.11|P-value:5.42E-4 ID:SGTC_1963|Compound:st076565|FD-Score:3.30|P-value:2.60E-4 ID:SGTC_1984|Compound:st072354|FD-Score:-3.35|P-value:2.74E-4 ID:SGTC_1989|Compound:st071868|FD-Score:3.92|P-value:1.97E-5 ID:SGTC_2000|Compound:st075254|FD-Score:-3.14|P-value:5.99E-4 ID:SGTC_2036|Compound:5160441|FD-Score:4.17|P-value:6.14E-6 ID:SGTC_2147|Compound:5545932|FD-Score:3.96|P-value:1.65E-5 ID:SGTC_2218|Compound:6965428|FD-Score:4.87|P-value:1.66E-7 ID:SGTC_2399|Compound:5884133|FD-Score:3.24|P-value:3.25E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:5.11|P-value:4.24E-8 ID:SGTC_2448|Compound:5185112|FD-Score:5.83|P-value:5.32E-10 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:3.58|P-value:8.52E-5 ID:SGTC_2720|Compound:fluspirilene|FD-Score:3.27|P-value:2.94E-4 ID:SGTC_2726|Compound:cimetidine|FD-Score:-3.54|P-value:1.26E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:-3.60|P-value:9.76E-5 ID:SGTC_2835|Compound:9006588|FD-Score:3.82|P-value:3.05E-5 ID:SGTC_2850|Compound:9015429|FD-Score:3.07|P-value:6.11E-4 ID:SGTC_2892|Compound:9047358|FD-Score:4.24|P-value:4.32E-6 ID:SGTC_2894|Compound:9048625|FD-Score:-4.97|P-value:1.31E-7 ID:SGTC_2930|Compound:9013230|FD-Score:3.15|P-value:4.65E-4 ID:SGTC_2937|Compound:9044753|FD-Score:3.98|P-value:1.50E-5 ID:SGTC_2946|Compound:9065502|FD-Score:-3.12|P-value:6.38E-4 ID:SGTC_2950|Compound:9072895|FD-Score:3.21|P-value:3.63E-4 ID:SGTC_3114|Compound:9122766|FD-Score:-3.28|P-value:3.54E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-3.69|P-value:6.83E-5 ID:SGTC_3158|Compound:9100007|FD-Score:4.49|P-value:1.24E-6 ID:SGTC_3159|Compound:9100096|FD-Score:3.23|P-value:3.44E-4

Top fitness defect scores for YOR148C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2448 5185112 5.83 27.2 µM 5.32E-10 3 2.85E-9 0.72
2 SGTC_2406 ethylparaben 5.11 405.7 µM 4.24E-8 3 1.60E-7 0.49
3 SGTC_2218 6965428 4.87 193.0 µM 1.66E-7 TSC3-RPN4 1 5.59E-7 1.34
4 SGTC_3158 9100007 4.49 49.5 µM 1.24E-6 3 3.55E-6 0.31
5 SGTC_2892 9047358 4.24 58.4 µM 4.32E-6 1 1.11E-5 0.39
6 SGTC_2036 5160441 4.17 105.0 µM 6.14E-6 NEO1 23 1.54E-5 0.52
7 SGTC_2937 9044753 3.98 11.3 µM 1.50E-5 60S ribosome export 7 3.49E-5 0.00
8 SGTC_2147 5545932 3.96 89.7 µM 1.65E-5 ergosterol biosynthesis 13 3.82E-5 0.24
9 SGTC_1989 st071868 3.92 50.2 µM 1.97E-5 6 4.49E-5 0.38
10 SGTC_1646 st011709 3.84 75.6 µM 2.78E-5 6 6.16E-5 0.00
11 SGTC_2835 9006588 3.82 71.4 µM 3.05E-5 9 6.72E-5 0.07
12 SGTC_903 1134-0042 3.65 114.0 µM 6.41E-5 8 1.33E-4 0.09
13 SGTC_2614 arthonioic acid 3.58 13.4 µM 8.52E-5 5 1.72E-4 0.35
14 SGTC_1780 st038448 3.48 24.5 µM 1.27E-4 iron homeostasis 11 2.49E-4 0.12
15 SGTC_1963 st076565 3.30 50.3 µM 2.60E-4 9 4.82E-4 0.04
16 SGTC_234 nsc-17383 3.28 10.7 µM 2.82E-4 ERG2 11 5.18E-4 0.03
17 SGTC_2720 fluspirilene 3.27 36.9 µM 2.94E-4 ERG2 7 5.39E-4 0.10
18 SGTC_2399 5884133 3.24 200.0 µM 3.25E-4 cell wall signaling 26 5.90E-4 0.09
19 SGTC_3159 9100096 3.23 49.5 µM 3.44E-4 13 6.22E-4 0.03
20 SGTC_2950 9072895 3.21 21.5 µM 3.63E-4 17 6.53E-4 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.125 3.99E-13 YPL146C NOP53 het Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired
0.096 2.89E-8 YDL209C CWC2 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
0.093 6.66E-8 YJR147W HMS2 hom Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication
0.092 8.51E-8 YDR415C_p YDR415C_p hom Putative aminopeptidase
0.092 9.27E-8 YOL130W ALR1 het Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions
0.089 2.17E-7 YCR054C CTR86 het Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress
0.084 9.73E-7 YDL053C PBP4 hom Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress
0.084 1.26E-6 YNL132W KRE33 het Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
0.082 1.77E-6 YPL021W ECM23 hom Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p
0.082 2.10E-6 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.079 4.67E-6 YGR040W KSS1 hom Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains
0.077 7.43E-6 YER136W GDI1 het GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
0.077 8.36E-6 YKR012C_d YKR012C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRY2
0.076 9.56E-6 YAL053W FLC2 hom Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
0.075 1.35E-5 YEL002C WBP1 het Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum