HIPHOP chemogenomics database

YOR174W / MED4

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation

Zygosity: Heterozygous strain

fixedexpanded
Profile for YOR174W / MED4

Click on Significant Values for Screen Details

Top fitness defect scores for YOR174W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_227	fluconazole	32.30	33.5 µM	1.43E-163	azole & statin	2	8.46E-230	10.50
2	SGTC_2249	haloperidol	15.10	116.4 µM	2.49E-37	ergosterol depletion effects on membrane	3	7.76E-52	0.06
3	SGTC_2248	tofa	12.80	1.1 µM	3.46E-27	sphingolipid biosynthesis & PDR1	4	1.48E-37	0.31
4	SGTC_2256	7954252	10.50	200.0 µM	3.74E-19	mitochondrial processes	1	2.98E-26	2.88
5	SGTC_217	ketoconazole	9.47	989.0 nM	8.14E-16	ergosterol depletion effects on membrane	1	1.46E-21	2.33
6	SGTC_1707	st032323	8.89	6.3 µM	3.64E-14	DNA damage response	9	3.05E-19	0.32
7	SGTC_1959	st077091	8.69	55.8 µM	1.29E-13	RPP1 & pyrimidine depletion	4	1.81E-18	0.39
8	SGTC_546	peri-Naphthalide	7.75	147.0 µM	3.53E-11	DNA damage response	2	4.74E-15	0.10
9	SGTC_453	enantio-paf c16	7.10	10.0 µM	1.17E-9		2	6.40E-13	0.69
10	SGTC_3327	9140648	6.84	26.9 µM	4.21E-9		2	3.85E-12	1.34
11	SGTC_829	1635-0120	6.80	117.0 µM	5.13E-9	DNA damage response	25	5.08E-12	0.59
12	SGTC_178	4092-0806	6.08	87.5 µM	1.57E-7	ergosterol depletion effects on membrane	10	6.06E-10	0.17
13	SGTC_3200	9114385	6.07	49.5 µM	1.63E-7		4	6.41E-10	1.03
14	SGTC_1067	clotrimazole	6.07	1.4 µM	1.65E-7	azole & statin	8	6.52E-10	0.72
15	SGTC_2109	5483026	6.05	200.0 µM	1.75E-7	RSC complex & mRNA processing	20	7.08E-10	0.41
16	SGTC_263	alizarin	5.72	107.9 µM	7.52E-7	iron homeostasis	4	5.42E-9	0.49
17	SGTC_2696	st077691	5.65	74.3 µM	9.82E-7		6	7.88E-9	0.12
18	SGTC_2043	5101342	5.62	149.0 µM	1.14E-6	SWF1 & branched chain AA biosynthesis	2	9.65E-9	0.71
19	SGTC_218	alverine citrate	5.56	93.8 µM	1.43E-6	fatty acid desaturase (OLE1)	6	1.33E-8	1.09
20	SGTC_2111	5486399	5.54	200.0 µM	1.55E-6		17	1.50E-8	0.41

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.296	1.11E-68	YJL195C_d	YJL195C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
0.225	1.27E-39	YCR093W	CDC39	het	Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
0.221	2.51E-38	YDR443C	SSN2	hom	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation
0.217	4.44E-37	YJL054W	TIM54	het	Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
0.212	2.71E-35	YFR037C	RSC8	het	Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
0.203	1.20E-32	YML127W	RSC9	het	Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
0.201	6.03E-32	YOL135C	MED7	het	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.195	5.18E-30	YGR122W	YGR122W	hom	Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
0.191	5.32E-29	YIL126W	STH1	het	ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
0.190	9.62E-29	YDL196W_d	YDL196W_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
0.186	1.22E-27	YPR034W	ARP7	het	Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
0.183	1.31E-26	YHR036W	BRL1	het	Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
0.173	6.53E-24	YML075C	HMG1	hom	HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
0.167	2.11E-22	YOR236W	DFR1	het	Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
0.165	5.57E-22	YKL028W	TFA1	het	TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening