YOR174W / MED4

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOR174W / MED4

Click on Significant Values for Screen Details ID:SGTC_103|Compound:0844-0104|FD-Score:4.10|P-value:2.82E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:3.94|P-value:4.58E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:-5.15|P-value:7.20E-6 ID:SGTC_156|Compound:3474-0145|FD-Score:3.91|P-value:4.97E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:6.08|P-value:1.57E-7 ID:SGTC_197|Compound:4544-0043|FD-Score:3.85|P-value:5.97E-4 ID:SGTC_217|Compound:ketoconazole|FD-Score:9.47|P-value:8.14E-16 ID:SGTC_218|Compound:alverine citrate|FD-Score:5.56|P-value:1.43E-6 ID:SGTC_227|Compound:fluconazole|FD-Score:32.30|P-value:1.43E-163 ID:SGTC_257|Compound:daunorubicin|FD-Score:4.41|P-value:1.05E-4 ID:SGTC_263|Compound:alizarin|FD-Score:5.72|P-value:7.52E-7 ID:SGTC_268|Compound:camptothecin|FD-Score:4.50|P-value:7.77E-5 ID:SGTC_298|Compound:1391-0729|FD-Score:4.26|P-value:1.69E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:4.21|P-value:2.02E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:4.72|P-value:3.62E-5 ID:SGTC_309|Compound:k408-0051|FD-Score:-4.09|P-value:2.83E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:3.69|P-value:9.60E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:3.83|P-value:6.37E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:4.41|P-value:1.05E-4 ID:SGTC_444|Compound:curcumin|FD-Score:4.01|P-value:3.74E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:7.10|P-value:1.17E-9 ID:SGTC_456|Compound:gingerol|FD-Score:4.22|P-value:1.91E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:5.44|P-value:2.31E-6 ID:SGTC_515|Compound:4092-1064|FD-Score:5.33|P-value:3.60E-6 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:7.75|P-value:3.53E-11 ID:SGTC_558|Compound:k064-0027|FD-Score:3.82|P-value:6.50E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:4.80|P-value:2.69E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.82|P-value:6.60E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:4.59|P-value:5.61E-5 ID:SGTC_829|Compound:1635-0120|FD-Score:6.80|P-value:5.13E-9 ID:SGTC_892|Compound:0929-0076|FD-Score:4.27|P-value:1.63E-4 ID:SGTC_895|Compound:0964-0006|FD-Score:5.05|P-value:1.07E-5 ID:SGTC_943|Compound:3253-1379|FD-Score:5.23|P-value:5.44E-6 ID:SGTC_946|Compound:3434-1334|FD-Score:-5.29|P-value:4.10E-6 ID:SGTC_952|Compound:1185-0122|FD-Score:-4.47|P-value:8.33E-5 ID:SGTC_1048|Compound:3161-0300|FD-Score:4.44|P-value:9.32E-5 ID:SGTC_1057|Compound:3448-5381|FD-Score:4.42|P-value:9.88E-5 ID:SGTC_1058|Compound:3448-5425|FD-Score:4.85|P-value:2.27E-5 ID:SGTC_1067|Compound:clotrimazole|FD-Score:6.07|P-value:1.65E-7 ID:SGTC_1084|Compound:voriconazole|FD-Score:3.90|P-value:5.11E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:4.18|P-value:2.19E-4 ID:SGTC_1168|Compound:k815-0004|FD-Score:3.96|P-value:4.33E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.69|P-value:9.43E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:4.32|P-value:1.37E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:4.53|P-value:6.84E-5 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:5.30|P-value:4.06E-6 ID:SGTC_1468|Compound:k284-1086|FD-Score:4.02|P-value:3.60E-4 ID:SGTC_1547|Compound:harmine|FD-Score:4.69|P-value:4.03E-5 ID:SGTC_1627|Compound:st003713|FD-Score:-3.96|P-value:4.22E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:4.01|P-value:3.74E-4 ID:SGTC_1707|Compound:st032323|FD-Score:8.89|P-value:3.64E-14 ID:SGTC_1715|Compound:st032160|FD-Score:3.75|P-value:8.10E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-5.39|P-value:2.82E-6 ID:SGTC_1745|Compound:st081495|FD-Score:-3.70|P-value:8.99E-4 ID:SGTC_1834|Compound:st055981|FD-Score:4.87|P-value:2.09E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:-5.23|P-value:5.29E-6 ID:SGTC_1921|Compound:5740136|FD-Score:5.47|P-value:2.05E-6 ID:SGTC_1950|Compound:st077106|FD-Score:-7.37|P-value:2.68E-10 ID:SGTC_1959|Compound:st077091|FD-Score:8.69|P-value:1.29E-13 ID:SGTC_1983|Compound:st071813|FD-Score:-4.02|P-value:3.57E-4 ID:SGTC_2041|Compound:5107442|FD-Score:3.75|P-value:8.11E-4 ID:SGTC_2043|Compound:5101342|FD-Score:5.62|P-value:1.14E-6 ID:SGTC_2068|Compound:5226020|FD-Score:3.75|P-value:8.11E-4 ID:SGTC_2075|Compound:5212009|FD-Score:3.76|P-value:7.79E-4 ID:SGTC_2082|Compound:5219693|FD-Score:-4.08|P-value:2.90E-4 ID:SGTC_2109|Compound:5483026|FD-Score:6.05|P-value:1.75E-7 ID:SGTC_2111|Compound:5486399|FD-Score:5.54|P-value:1.55E-6 ID:SGTC_2248|Compound:tofa|FD-Score:12.80|P-value:3.46E-27 ID:SGTC_2249|Compound:haloperidol|FD-Score:15.10|P-value:2.49E-37 ID:SGTC_2256|Compound:7954252|FD-Score:10.50|P-value:3.74E-19 ID:SGTC_2257|Compound:7960413|FD-Score:4.17|P-value:2.26E-4 ID:SGTC_2326|Compound:6164455|FD-Score:5.03|P-value:1.18E-5 ID:SGTC_2344|Compound:7990960|FD-Score:4.30|P-value:1.47E-4 ID:SGTC_2556|Compound:citropten|FD-Score:4.68|P-value:4.15E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:-4.56|P-value:6.12E-5 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:4.12|P-value:2.63E-4 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:4.66|P-value:4.45E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.34|P-value:1.33E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.41|P-value:2.69E-6 ID:SGTC_2671|Compound:danthron|FD-Score:5.22|P-value:5.52E-6 ID:SGTC_2679|Compound:rapamycin|FD-Score:-5.42|P-value:2.47E-6 ID:SGTC_2696|Compound:st077691|FD-Score:5.65|P-value:9.82E-7 ID:SGTC_2756|Compound:carbadox|FD-Score:-3.92|P-value:4.83E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.92|P-value:4.77E-4 ID:SGTC_2896|Compound:9052656|FD-Score:4.59|P-value:5.56E-5 ID:SGTC_2947|Compound:9072639|FD-Score:4.95|P-value:1.56E-5 ID:SGTC_3200|Compound:9114385|FD-Score:6.07|P-value:1.63E-7 ID:SGTC_3266|Compound:9136860|FD-Score:-4.20|P-value:2.01E-4 ID:SGTC_3318|Compound:9139014|FD-Score:3.73|P-value:8.49E-4 ID:SGTC_3327|Compound:9140648|FD-Score:6.84|P-value:4.21E-9 ID:SGTC_103|Compound:0844-0104|FD-Score:4.10|P-value:2.82E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:3.94|P-value:4.58E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:-5.15|P-value:7.20E-6 ID:SGTC_156|Compound:3474-0145|FD-Score:3.91|P-value:4.97E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:6.08|P-value:1.57E-7 ID:SGTC_197|Compound:4544-0043|FD-Score:3.85|P-value:5.97E-4 ID:SGTC_217|Compound:ketoconazole|FD-Score:9.47|P-value:8.14E-16 ID:SGTC_218|Compound:alverine citrate|FD-Score:5.56|P-value:1.43E-6 ID:SGTC_227|Compound:fluconazole|FD-Score:32.30|P-value:1.43E-163 ID:SGTC_257|Compound:daunorubicin|FD-Score:4.41|P-value:1.05E-4 ID:SGTC_263|Compound:alizarin|FD-Score:5.72|P-value:7.52E-7 ID:SGTC_268|Compound:camptothecin|FD-Score:4.50|P-value:7.77E-5 ID:SGTC_298|Compound:1391-0729|FD-Score:4.26|P-value:1.69E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:4.21|P-value:2.02E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:4.72|P-value:3.62E-5 ID:SGTC_309|Compound:k408-0051|FD-Score:-4.09|P-value:2.83E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:3.69|P-value:9.60E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:3.83|P-value:6.37E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:4.41|P-value:1.05E-4 ID:SGTC_444|Compound:curcumin|FD-Score:4.01|P-value:3.74E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:7.10|P-value:1.17E-9 ID:SGTC_456|Compound:gingerol|FD-Score:4.22|P-value:1.91E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:5.44|P-value:2.31E-6 ID:SGTC_515|Compound:4092-1064|FD-Score:5.33|P-value:3.60E-6 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:7.75|P-value:3.53E-11 ID:SGTC_558|Compound:k064-0027|FD-Score:3.82|P-value:6.50E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:4.80|P-value:2.69E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.82|P-value:6.60E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:4.59|P-value:5.61E-5 ID:SGTC_829|Compound:1635-0120|FD-Score:6.80|P-value:5.13E-9 ID:SGTC_892|Compound:0929-0076|FD-Score:4.27|P-value:1.63E-4 ID:SGTC_895|Compound:0964-0006|FD-Score:5.05|P-value:1.07E-5 ID:SGTC_943|Compound:3253-1379|FD-Score:5.23|P-value:5.44E-6 ID:SGTC_946|Compound:3434-1334|FD-Score:-5.29|P-value:4.10E-6 ID:SGTC_952|Compound:1185-0122|FD-Score:-4.47|P-value:8.33E-5 ID:SGTC_1048|Compound:3161-0300|FD-Score:4.44|P-value:9.32E-5 ID:SGTC_1057|Compound:3448-5381|FD-Score:4.42|P-value:9.88E-5 ID:SGTC_1058|Compound:3448-5425|FD-Score:4.85|P-value:2.27E-5 ID:SGTC_1067|Compound:clotrimazole|FD-Score:6.07|P-value:1.65E-7 ID:SGTC_1084|Compound:voriconazole|FD-Score:3.90|P-value:5.11E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:4.18|P-value:2.19E-4 ID:SGTC_1168|Compound:k815-0004|FD-Score:3.96|P-value:4.33E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.69|P-value:9.43E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:4.32|P-value:1.37E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:4.53|P-value:6.84E-5 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:5.30|P-value:4.06E-6 ID:SGTC_1468|Compound:k284-1086|FD-Score:4.02|P-value:3.60E-4 ID:SGTC_1547|Compound:harmine|FD-Score:4.69|P-value:4.03E-5 ID:SGTC_1627|Compound:st003713|FD-Score:-3.96|P-value:4.22E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:4.01|P-value:3.74E-4 ID:SGTC_1707|Compound:st032323|FD-Score:8.89|P-value:3.64E-14 ID:SGTC_1715|Compound:st032160|FD-Score:3.75|P-value:8.10E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-5.39|P-value:2.82E-6 ID:SGTC_1745|Compound:st081495|FD-Score:-3.70|P-value:8.99E-4 ID:SGTC_1834|Compound:st055981|FD-Score:4.87|P-value:2.09E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:-5.23|P-value:5.29E-6 ID:SGTC_1921|Compound:5740136|FD-Score:5.47|P-value:2.05E-6 ID:SGTC_1950|Compound:st077106|FD-Score:-7.37|P-value:2.68E-10 ID:SGTC_1959|Compound:st077091|FD-Score:8.69|P-value:1.29E-13 ID:SGTC_1983|Compound:st071813|FD-Score:-4.02|P-value:3.57E-4 ID:SGTC_2041|Compound:5107442|FD-Score:3.75|P-value:8.11E-4 ID:SGTC_2043|Compound:5101342|FD-Score:5.62|P-value:1.14E-6 ID:SGTC_2068|Compound:5226020|FD-Score:3.75|P-value:8.11E-4 ID:SGTC_2075|Compound:5212009|FD-Score:3.76|P-value:7.79E-4 ID:SGTC_2082|Compound:5219693|FD-Score:-4.08|P-value:2.90E-4 ID:SGTC_2109|Compound:5483026|FD-Score:6.05|P-value:1.75E-7 ID:SGTC_2111|Compound:5486399|FD-Score:5.54|P-value:1.55E-6 ID:SGTC_2248|Compound:tofa|FD-Score:12.80|P-value:3.46E-27 ID:SGTC_2249|Compound:haloperidol|FD-Score:15.10|P-value:2.49E-37 ID:SGTC_2256|Compound:7954252|FD-Score:10.50|P-value:3.74E-19 ID:SGTC_2257|Compound:7960413|FD-Score:4.17|P-value:2.26E-4 ID:SGTC_2326|Compound:6164455|FD-Score:5.03|P-value:1.18E-5 ID:SGTC_2344|Compound:7990960|FD-Score:4.30|P-value:1.47E-4 ID:SGTC_2556|Compound:citropten|FD-Score:4.68|P-value:4.15E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:-4.56|P-value:6.12E-5 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:4.12|P-value:2.63E-4 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:4.66|P-value:4.45E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.34|P-value:1.33E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.41|P-value:2.69E-6 ID:SGTC_2671|Compound:danthron|FD-Score:5.22|P-value:5.52E-6 ID:SGTC_2679|Compound:rapamycin|FD-Score:-5.42|P-value:2.47E-6 ID:SGTC_2696|Compound:st077691|FD-Score:5.65|P-value:9.82E-7 ID:SGTC_2756|Compound:carbadox|FD-Score:-3.92|P-value:4.83E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.92|P-value:4.77E-4 ID:SGTC_2896|Compound:9052656|FD-Score:4.59|P-value:5.56E-5 ID:SGTC_2947|Compound:9072639|FD-Score:4.95|P-value:1.56E-5 ID:SGTC_3200|Compound:9114385|FD-Score:6.07|P-value:1.63E-7 ID:SGTC_3266|Compound:9136860|FD-Score:-4.20|P-value:2.01E-4 ID:SGTC_3318|Compound:9139014|FD-Score:3.73|P-value:8.49E-4 ID:SGTC_3327|Compound:9140648|FD-Score:6.84|P-value:4.21E-9

Top fitness defect scores for YOR174W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_227 fluconazole 32.30 33.5 µM 1.43E-163 azole & statin 2 8.46E-230 10.50
2 SGTC_2249 haloperidol 15.10 116.4 µM 2.49E-37 ergosterol depletion effects on membrane 3 7.76E-52 0.06
3 SGTC_2248 tofa 12.80 1.1 µM 3.46E-27 sphingolipid biosynthesis & PDR1 4 1.48E-37 0.31
4 SGTC_2256 7954252 10.50 200.0 µM 3.74E-19 mitochondrial processes 1 2.98E-26 2.88
5 SGTC_217 ketoconazole 9.47 989.0 nM 8.14E-16 ergosterol depletion effects on membrane 1 1.46E-21 2.33
6 SGTC_1707 st032323 8.89 6.3 µM 3.64E-14 DNA damage response 9 3.05E-19 0.32
7 SGTC_1959 st077091 8.69 55.8 µM 1.29E-13 RPP1 & pyrimidine depletion 4 1.81E-18 0.39
8 SGTC_546 peri-Naphthalide 7.75 147.0 µM 3.53E-11 DNA damage response 2 4.74E-15 0.10
9 SGTC_453 enantio-paf c16 7.10 10.0 µM 1.17E-9 2 6.40E-13 0.69
10 SGTC_3327 9140648 6.84 26.9 µM 4.21E-9 2 3.85E-12 1.34
11 SGTC_829 1635-0120 6.80 117.0 µM 5.13E-9 DNA damage response 25 5.08E-12 0.59
12 SGTC_178 4092-0806 6.08 87.5 µM 1.57E-7 ergosterol depletion effects on membrane 10 6.06E-10 0.17
13 SGTC_3200 9114385 6.07 49.5 µM 1.63E-7 4 6.41E-10 1.03
14 SGTC_1067 clotrimazole 6.07 1.4 µM 1.65E-7 azole & statin 8 6.52E-10 0.72
15 SGTC_2109 5483026 6.05 200.0 µM 1.75E-7 RSC complex & mRNA processing 20 7.08E-10 0.41
16 SGTC_263 alizarin 5.72 107.9 µM 7.52E-7 iron homeostasis 4 5.42E-9 0.49
17 SGTC_2696 st077691 5.65 74.3 µM 9.82E-7 6 7.88E-9 0.12
18 SGTC_2043 5101342 5.62 149.0 µM 1.14E-6 SWF1 & branched chain AA biosynthesis 2 9.65E-9 0.71
19 SGTC_218 alverine citrate 5.56 93.8 µM 1.43E-6 fatty acid desaturase (OLE1) 6 1.33E-8 1.09
20 SGTC_2111 5486399 5.54 200.0 µM 1.55E-6 17 1.50E-8 0.41

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.296 1.11E-68 YJL195C_d YJL195C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
0.225 1.27E-39 YCR093W CDC39 het Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
0.221 2.51E-38 YDR443C SSN2 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation
0.217 4.44E-37 YJL054W TIM54 het Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
0.212 2.71E-35 YFR037C RSC8 het Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
0.203 1.20E-32 YML127W RSC9 het Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
0.201 6.03E-32 YOL135C MED7 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.195 5.18E-30 YGR122W YGR122W hom Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
0.191 5.32E-29 YIL126W STH1 het ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
0.190 9.62E-29 YDL196W_d YDL196W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
0.186 1.22E-27 YPR034W ARP7 het Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
0.183 1.31E-26 YHR036W BRL1 het Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
0.173 6.53E-24 YML075C HMG1 hom HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
0.167 2.11E-22 YOR236W DFR1 het Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
0.165 5.57E-22 YKL028W TFA1 het TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening