YOR176W / HEM15

Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOR176W / HEM15

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.63|P-value:1.95E-4 ID:SGTC_13|Compound:0199-0087|FD-Score:3.22|P-value:8.38E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:-4.86|P-value:6.63E-7 ID:SGTC_57|Compound:miconazole|FD-Score:-4.65|P-value:1.89E-6 ID:SGTC_61|Compound:0978-8327|FD-Score:-3.29|P-value:5.10E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:3.61|P-value:2.16E-4 ID:SGTC_288|Compound:1315-0019|FD-Score:5.25|P-value:1.38E-7 ID:SGTC_311|Compound:1315-0136|FD-Score:3.45|P-value:3.84E-4 ID:SGTC_348|Compound:k064-0012|FD-Score:4.42|P-value:7.70E-6 ID:SGTC_350|Compound:k064-0020|FD-Score:-6.98|P-value:2.11E-12 ID:SGTC_399|Compound:oxolinic acid|FD-Score:3.33|P-value:5.90E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:6.33|P-value:2.71E-10 ID:SGTC_771|Compound:0250-0039|FD-Score:3.77|P-value:1.15E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-5.29|P-value:7.26E-8 ID:SGTC_902|Compound:1120-0002|FD-Score:3.74|P-value:1.33E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:3.43|P-value:4.16E-4 ID:SGTC_1027|Compound:k015-0025|FD-Score:-3.99|P-value:3.50E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-4.30|P-value:9.46E-6 ID:SGTC_1044|Compound:1222-0021|FD-Score:4.11|P-value:2.92E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:9.69|P-value:8.34E-22 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.92|P-value:6.44E-5 ID:SGTC_1555|Compound:citronellol|FD-Score:3.62|P-value:2.06E-4 ID:SGTC_1608|Compound:st001473|FD-Score:4.34|P-value:1.09E-5 ID:SGTC_1626|Compound:st003712|FD-Score:5.98|P-value:2.23E-9 ID:SGTC_1905|Compound:st060207|FD-Score:3.73|P-value:1.35E-4 ID:SGTC_1913|Compound:st060213|FD-Score:-3.46|P-value:2.75E-4 ID:SGTC_2018|Compound:5133068|FD-Score:6.21|P-value:5.62E-10 ID:SGTC_2023|Compound:5144124|FD-Score:3.59|P-value:2.30E-4 ID:SGTC_2025|Compound:5147754|FD-Score:-3.47|P-value:2.69E-4 ID:SGTC_2039|Compound:Aminomethylbenzodioxan|FD-Score:3.37|P-value:5.11E-4 ID:SGTC_2269|Compound:7935389|FD-Score:-3.10|P-value:9.80E-4 ID:SGTC_2294|Compound:7971431|FD-Score:3.20|P-value:9.08E-4 ID:SGTC_2439|Compound:5492030|FD-Score:-3.74|P-value:9.70E-5 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-3.17|P-value:7.69E-4 ID:SGTC_2898|Compound:9056270|FD-Score:-3.32|P-value:4.55E-4 ID:SGTC_3005|Compound:9076496|FD-Score:3.76|P-value:1.23E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.63|P-value:1.95E-4 ID:SGTC_13|Compound:0199-0087|FD-Score:3.22|P-value:8.38E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:-4.86|P-value:6.63E-7 ID:SGTC_57|Compound:miconazole|FD-Score:-4.65|P-value:1.89E-6 ID:SGTC_61|Compound:0978-8327|FD-Score:-3.29|P-value:5.10E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:3.61|P-value:2.16E-4 ID:SGTC_288|Compound:1315-0019|FD-Score:5.25|P-value:1.38E-7 ID:SGTC_311|Compound:1315-0136|FD-Score:3.45|P-value:3.84E-4 ID:SGTC_348|Compound:k064-0012|FD-Score:4.42|P-value:7.70E-6 ID:SGTC_350|Compound:k064-0020|FD-Score:-6.98|P-value:2.11E-12 ID:SGTC_399|Compound:oxolinic acid|FD-Score:3.33|P-value:5.90E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:6.33|P-value:2.71E-10 ID:SGTC_771|Compound:0250-0039|FD-Score:3.77|P-value:1.15E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-5.29|P-value:7.26E-8 ID:SGTC_902|Compound:1120-0002|FD-Score:3.74|P-value:1.33E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:3.43|P-value:4.16E-4 ID:SGTC_1027|Compound:k015-0025|FD-Score:-3.99|P-value:3.50E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-4.30|P-value:9.46E-6 ID:SGTC_1044|Compound:1222-0021|FD-Score:4.11|P-value:2.92E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:9.69|P-value:8.34E-22 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.92|P-value:6.44E-5 ID:SGTC_1555|Compound:citronellol|FD-Score:3.62|P-value:2.06E-4 ID:SGTC_1608|Compound:st001473|FD-Score:4.34|P-value:1.09E-5 ID:SGTC_1626|Compound:st003712|FD-Score:5.98|P-value:2.23E-9 ID:SGTC_1905|Compound:st060207|FD-Score:3.73|P-value:1.35E-4 ID:SGTC_1913|Compound:st060213|FD-Score:-3.46|P-value:2.75E-4 ID:SGTC_2018|Compound:5133068|FD-Score:6.21|P-value:5.62E-10 ID:SGTC_2023|Compound:5144124|FD-Score:3.59|P-value:2.30E-4 ID:SGTC_2025|Compound:5147754|FD-Score:-3.47|P-value:2.69E-4 ID:SGTC_2039|Compound:Aminomethylbenzodioxan|FD-Score:3.37|P-value:5.11E-4 ID:SGTC_2269|Compound:7935389|FD-Score:-3.10|P-value:9.80E-4 ID:SGTC_2294|Compound:7971431|FD-Score:3.20|P-value:9.08E-4 ID:SGTC_2439|Compound:5492030|FD-Score:-3.74|P-value:9.70E-5 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-3.17|P-value:7.69E-4 ID:SGTC_2898|Compound:9056270|FD-Score:-3.32|P-value:4.55E-4 ID:SGTC_3005|Compound:9076496|FD-Score:3.76|P-value:1.23E-4

Top fitness defect scores for YOR176W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1310 1188-0297 9.69 994.0 nM 8.34E-22 azole & statin 2 1.63E-22 2.63
2 SGTC_547 1120-0019 6.33 610.0 nM 2.71E-10 azole & statin 2 1.23E-10 1.01
3 SGTC_2018 5133068 6.21 143.0 µM 5.62E-10 3 2.60E-10 0.34
4 SGTC_1626 st003712 5.98 1.8 µM 2.23E-9 4 1.08E-9 1.46
5 SGTC_288 1315-0019 5.25 35.1 µM 1.38E-7 RSC & ERG11 10 7.68E-8 0.09
6 SGTC_348 k064-0012 4.42 10.7 µM 7.70E-6 60S ribosome export 1 4.91E-6 0.24
7 SGTC_1608 st001473 4.34 40.5 µM 1.09E-5 3 7.02E-6 0.32
8 SGTC_1044 1222-0021 4.11 1.8 µM 2.92E-5 heme biosynthesis & mitochondrial translocase 33 1.95E-5 0.07
9 SGTC_1473 k844-0725 3.92 134.0 µM 6.44E-5 5 4.42E-5 0.22
10 SGTC_771 0250-0039 3.77 27.3 µM 1.15E-4 3 8.05E-5 0.17
11 SGTC_3005 9076496 3.76 71.4 µM 1.23E-4 1 8.64E-5 0.36
12 SGTC_902 1120-0002 3.74 2.1 µM 1.33E-4 azole & statin 21 9.38E-5 0.20
13 SGTC_1905 st060207 3.73 84.5 µM 1.35E-4 4 9.53E-5 0.38
14 SGTC_6 0844-0013 3.63 19.8 µM 1.95E-4 12 1.39E-4 0.03
15 SGTC_1555 citronellol 3.62 128.0 µM 2.06E-4 3 1.47E-4 0.16
16 SGTC_157 1348-1695 3.61 65.8 µM 2.16E-4 iron homeostasis 39 1.55E-4 0.04
17 SGTC_2023 5144124 3.59 124.0 µM 2.30E-4 9 1.65E-4 0.04
18 SGTC_311 1315-0136 3.45 58.0 µM 3.84E-4 9 2.81E-4 0.03
19 SGTC_995 3931-2021 3.43 34.6 µM 4.16E-4 RPP1 & pyrimidine depletion 11 3.06E-4 0.26
20 SGTC_2039 Aminomethylbenzodioxan 3.37 200.0 µM 5.11E-4 4 3.78E-4 0.12

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.166 3.57E-22 YDL123W SNA4 hom Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated
0.127 1.57E-13 YDR223W CRF1 hom Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain
0.125 3.13E-13 YPL228W CET1 het Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
0.118 6.45E-12 YDL205C HEM3 het Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
0.112 6.63E-11 YHR021W-A_p ECM12_p hom Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.109 2.33E-10 YBL103C RTG3 hom Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways
0.107 6.09E-10 YGR270W YTA7 hom Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p
0.103 2.02E-9 YOL067C RTG1 hom Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus
0.103 2.38E-9 YPR082C DIB1 het 17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein
0.098 1.40E-8 YGR199W PMT6 hom Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases
0.097 1.81E-8 YMR043W MCM1 het Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes
0.096 2.27E-8 YBR035C PDX3 hom Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism
0.095 3.05E-8 YOR155C ISN1 hom Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.092 8.24E-8 YLR103C CDC45 het DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes
0.091 1.32E-7 YKR071C DRE2 het Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress