HIPHOP chemogenomics database

YOR205C / GEP3

Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1)

Zygosity: Homozygous strain

fixedexpanded
Profile for YOR205C / GEP3

Click on Significant Values for Screen Details

Top fitness defect scores for YOR205C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1622	st003707	9.48	19.4 µM	1.22E-20	calcium & mitochondrial duress	5	1.22E-21
2	SGTC_236	1509-0011	8.20	5.3 µM	6.37E-16		244	1.19E-16
3	SGTC_173	1273-0061	7.00	2.7 µM	4.23E-12	mitochondrial stress	86	1.30E-12
4	SGTC_10	0279-0212	6.85	42.6 µM	1.11E-11	TRP & mitochondrial translation	15	3.58E-12
5	SGTC_1627	st003713	6.37	25.6 µM	2.42E-10	calcium & mitochondrial duress	25	9.29E-11
6	SGTC_385	1494-0393	5.86	12.4 µM	5.20E-9		1	2.35E-9
7	SGTC_1624	st003710	5.80	6.2 µM	7.37E-9	calcium & mitochondrial duress	16	3.40E-9
8	SGTC_1051	3253-3903	5.73	78.0 µM	1.09E-8		3	5.15E-9
9	SGTC_831	k061-1017	5.61	217.0 µM	2.06E-8	translation	14	1.01E-8
10	SGTC_1052	3253-3998	5.45	198.0 µM	4.82E-8		3	2.46E-8
11	SGTC_63	0099-0242	5.36	26.7 µM	7.97E-8	mitochondrial stress	44	4.18E-8
12	SGTC_3157	9099274	5.31	49.5 µM	1.03E-7		4	5.48E-8
13	SGTC_314	1671-0008	5.08	2.7 µM	3.40E-7		19	1.92E-7
14	SGTC_832	4083-0019	4.66	164.0 µM	2.48E-6	translation	54	1.56E-6
15	SGTC_310	1273-0058	4.59	1.1 µM	3.51E-6	mitochondrial stress	203	2.25E-6
16	SGTC_1329	1447-1697	4.56	50.6 µM	3.99E-6		9	2.57E-6
17	SGTC_727	3032-0951	4.50	202.0 µM	5.13E-6		20	3.34E-6
18	SGTC_2232	chlorophetanol	4.50	41.5 µM	5.26E-6	TRP & mitochondrial translation	99	3.43E-6
19	SGTC_1921	5740136	4.44	50.0 µM	6.66E-6	TRP & mitochondrial translation	42	4.40E-6
20	SGTC_2622	2-methoxyxanthone	4.43	59.9 µM	7.24E-6		9	4.80E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.387	2.00E-120	YOL095C	HMI1	hom	Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD
0.373	1.25E-110	YMR287C	DSS1	hom	3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.372	1.71E-110	YMR228W	MTF1	hom	Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.369	6.64E-109	YPL040C	ISM1	hom	Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
0.366	1.22E-106	YPR099C_d	YPR099C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W
0.364	8.02E-106	YNL184C_p	YNL184C_p	hom	Protein of unknown function; expressed at both mRNA and protein levels
0.364	1.73E-105	YPR166C	MRP2	hom	Mitochondrial ribosomal protein of the small subunit
0.362	1.14E-104	YMR193W	MRPL24	hom	Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
0.359	1.12E-102	YPL029W	SUV3	hom	ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron
0.355	1.93E-100	YDL056W	MBP1	hom	Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes
0.355	2.17E-100	YEL050C	RML2	hom	Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor
0.353	2.94E-99	YDR296W	MHR1	hom	Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress
0.347	1.54E-95	YGR219W_d	YGR219W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C
0.345	1.15E-94	YDR194C	MSS116	hom	DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing
0.338	1.97E-90	YDR462W	MRPL28	hom	Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress