HIPHOP chemogenomics database

YOR224C / RPB8

RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III

Zygosity: Heterozygous strain

fixedexpanded
Profile for YOR224C / RPB8

Click on Significant Values for Screen Details

Top fitness defect scores for YOR224C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_2671	danthron	6.86	100.0 µM	9.19E-7	DNA damage response	5	3.39E-12	0.47
2	SGTC_102	gallobenzophenone	6.82	83.6 µM	1.07E-6	redox potentiating	2	4.59E-12	2.29
3	SGTC_2544	sphondin	6.33	68.5 µM	5.40E-6	RNA pol III & RNase P/MRP	16	1.22E-10	0.16
4	SGTC_2206	7254556	5.92	147.0 µM	1.91E-5	60S ribosome export	2	1.57E-9	1.02
5	SGTC_221	trifluoperazine	5.87	10.3 µM	2.24E-5	NEO1-PIK1	16	2.16E-9	0.10
6	SGTC_1062	mesoridazine	5.68	14.2 µM	3.98E-5		2	6.91E-9	1.55
7	SGTC_2402	5553605	5.66	200.0 µM	4.11E-5		1	7.36E-9	1.18
8	SGTC_2393	9031399	5.66	185.9 µM	4.18E-5	RNA processing & uracil transport	3	7.62E-9	0.32
9	SGTC_93	0109-0045	5.64	59.2 µM	4.38E-5	DNA damage response	3	8.35E-9	0.12
10	SGTC_2467	5474540	5.61	200.0 µM	4.79E-5		1	9.99E-9	0.90
11	SGTC_2525	harmine	5.57	47.9 µM	5.44E-5	ergosterol biosynthesis	2	1.30E-8	0.31
12	SGTC_2216	7189485	5.38	200.0 µM	9.22E-5		1	3.74E-8	0.99
13	SGTC_2229	7354935	5.36	190.0 µM	9.69E-5	RSC complex & mRNA processing	14	4.13E-8	0.37
14	SGTC_648	0375-0473	5.27	33.3 µM	1.24E-4		2	6.74E-8	0.63
15	SGTC_2699	st077704	5.15	54.6 µM	1.72E-4	endomembrane recycling	3	1.31E-7	0.66
16	SGTC_3124	9127567	5.01	49.5 µM	2.46E-4	DNA damage response	1	2.67E-7	0.50
17	SGTC_1834	st055981	5.01	52.8 µM	2.52E-4	RPP1 & pyrimidine depletion	4	2.79E-7	0.14
18	SGTC_503	tyrphostin 8	4.87	294.0 µM	3.57E-4	TSC3-RPN4	6	5.63E-7	0.50
19	SGTC_91	0417-1751	4.86	58.4 µM	3.61E-4	Golgi	8	5.76E-7	0.42
20	SGTC_234	nsc-17383	4.84	10.7 µM	3.82E-4	ERG2	2	6.43E-7	0.72

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.155	1.75E-19	YDL150W	RPC53	het	RNA polymerase III subunit C53
0.154	2.80E-19	YOR116C	RPO31	het	RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.138	9.42E-16	YHL015W	RPS20	het	Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
0.131	2.87E-14	YOR157C	PUP1	het	Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
0.124	6.40E-13	YPL237W	SUI3	het	Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
0.124	7.75E-13	YPL142C_d	YPL142C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
0.122	1.46E-12	YOR319W	HSH49	het	U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
0.121	2.10E-12	YML031W	NDC1	het	Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
0.120	3.89E-12	YBR154C	RPB5	het	RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
0.117	1.31E-11	YOR340C	RPA43	het	RNA polymerase I subunit A43
0.115	2.78E-11	YOL040C	RPS15	het	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
0.114	3.72E-11	YDR225W	HTA1	hom	Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
0.107	5.65E-10	YDR429C	TIF35	het	eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
0.106	6.70E-10	YPR136C_d	YPR136C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
0.105	9.56E-10	YER025W	GCD11	het	Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met