YOR224C / RPB8

RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOR224C / RPB8

Click on Significant Values for Screen Details ID:SGTC_84|Compound:0443-0269|FD-Score:-4.85|P-value:3.68E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:-5.83|P-value:2.54E-5 ID:SGTC_91|Compound:0417-1751|FD-Score:4.86|P-value:3.61E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:5.64|P-value:4.38E-5 ID:SGTC_101|Compound:0490-5701|FD-Score:-4.78|P-value:4.39E-4 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:6.82|P-value:1.07E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.87|P-value:2.24E-5 ID:SGTC_234|Compound:nsc-17383|FD-Score:4.84|P-value:3.82E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:-4.47|P-value:9.42E-4 ID:SGTC_284|Compound:0988-0104|FD-Score:-5.33|P-value:1.06E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:-5.23|P-value:1.38E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:-5.72|P-value:3.49E-5 ID:SGTC_466|Compound:geldanamycin|FD-Score:-7.88|P-value:2.16E-8 ID:SGTC_490|Compound:phenamil|FD-Score:-4.84|P-value:3.79E-4 ID:SGTC_500|Compound:h-89|FD-Score:4.47|P-value:9.39E-4 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:4.87|P-value:3.57E-4 ID:SGTC_639|Compound:0833-0042|FD-Score:-5.87|P-value:2.22E-5 ID:SGTC_648|Compound:0375-0473|FD-Score:5.27|P-value:1.24E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:-5.74|P-value:3.32E-5 ID:SGTC_799|Compound:1310-0557|FD-Score:-4.67|P-value:5.89E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:-5.74|P-value:3.28E-5 ID:SGTC_936|Compound:3005-4620|FD-Score:-6.30|P-value:5.93E-6 ID:SGTC_949|Compound:1165-0505|FD-Score:4.73|P-value:5.06E-4 ID:SGTC_1062|Compound:mesoridazine|FD-Score:5.68|P-value:3.98E-5 ID:SGTC_1105|Compound:epirubicin|FD-Score:4.45|P-value:9.93E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:-6.49|P-value:3.18E-6 ID:SGTC_1255|Compound:0566-0045|FD-Score:-4.77|P-value:4.59E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:4.77|P-value:4.62E-4 ID:SGTC_1643|Compound:st009866|FD-Score:-4.68|P-value:5.66E-4 ID:SGTC_1742|Compound:st038235|FD-Score:-5.07|P-value:2.14E-4 ID:SGTC_1784|Compound:st039334|FD-Score:-4.62|P-value:6.56E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-5.06|P-value:2.17E-4 ID:SGTC_1834|Compound:st055981|FD-Score:5.01|P-value:2.52E-4 ID:SGTC_1853|Compound:st056710|FD-Score:4.76|P-value:4.63E-4 ID:SGTC_2206|Compound:7254556|FD-Score:5.92|P-value:1.91E-5 ID:SGTC_2216|Compound:7189485|FD-Score:5.38|P-value:9.22E-5 ID:SGTC_2229|Compound:7354935|FD-Score:5.36|P-value:9.69E-5 ID:SGTC_2393|Compound:9031399|FD-Score:5.66|P-value:4.18E-5 ID:SGTC_2399|Compound:5884133|FD-Score:-5.99|P-value:1.53E-5 ID:SGTC_2402|Compound:5553605|FD-Score:5.66|P-value:4.11E-5 ID:SGTC_2467|Compound:5474540|FD-Score:5.61|P-value:4.79E-5 ID:SGTC_2525|Compound:harmine|FD-Score:5.57|P-value:5.44E-5 ID:SGTC_2526|Compound:patulin|FD-Score:-5.28|P-value:1.20E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:6.33|P-value:5.40E-6 ID:SGTC_2611|Compound:pyromycin|FD-Score:4.73|P-value:5.10E-4 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:4.79|P-value:4.29E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:-4.65|P-value:6.05E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:-6.54|P-value:2.74E-6 ID:SGTC_2671|Compound:danthron|FD-Score:6.86|P-value:9.19E-7 ID:SGTC_2689|Compound:tacrolimus|FD-Score:-5.45|P-value:7.45E-5 ID:SGTC_2699|Compound:st077704|FD-Score:5.15|P-value:1.72E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:-14.10|P-value:5.59E-23 ID:SGTC_2778|Compound:tioconazole|FD-Score:-12.60|P-value:1.18E-18 ID:SGTC_2779|Compound:methotrexate|FD-Score:-6.49|P-value:3.20E-6 ID:SGTC_2854|Compound:9024559|FD-Score:-6.29|P-value:6.15E-6 ID:SGTC_2856|Compound:9022925|FD-Score:-5.15|P-value:1.71E-4 ID:SGTC_2921|Compound:7974240|FD-Score:-5.69|P-value:3.81E-5 ID:SGTC_2966|Compound:9084041|FD-Score:-5.63|P-value:4.48E-5 ID:SGTC_3124|Compound:9127567|FD-Score:5.01|P-value:2.46E-4 ID:SGTC_3187|Compound:9109394|FD-Score:-5.00|P-value:2.55E-4 ID:SGTC_3200|Compound:9114385|FD-Score:-4.96|P-value:2.83E-4 ID:SGTC_3204|Compound:9114183|FD-Score:-5.11|P-value:1.89E-4 ID:SGTC_3329|Compound:9141233|FD-Score:-5.00|P-value:2.52E-4 ID:SGTC_84|Compound:0443-0269|FD-Score:-4.85|P-value:3.68E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:-5.83|P-value:2.54E-5 ID:SGTC_91|Compound:0417-1751|FD-Score:4.86|P-value:3.61E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:5.64|P-value:4.38E-5 ID:SGTC_101|Compound:0490-5701|FD-Score:-4.78|P-value:4.39E-4 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:6.82|P-value:1.07E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.87|P-value:2.24E-5 ID:SGTC_234|Compound:nsc-17383|FD-Score:4.84|P-value:3.82E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:-4.47|P-value:9.42E-4 ID:SGTC_284|Compound:0988-0104|FD-Score:-5.33|P-value:1.06E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:-5.23|P-value:1.38E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:-5.72|P-value:3.49E-5 ID:SGTC_466|Compound:geldanamycin|FD-Score:-7.88|P-value:2.16E-8 ID:SGTC_490|Compound:phenamil|FD-Score:-4.84|P-value:3.79E-4 ID:SGTC_500|Compound:h-89|FD-Score:4.47|P-value:9.39E-4 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:4.87|P-value:3.57E-4 ID:SGTC_639|Compound:0833-0042|FD-Score:-5.87|P-value:2.22E-5 ID:SGTC_648|Compound:0375-0473|FD-Score:5.27|P-value:1.24E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:-5.74|P-value:3.32E-5 ID:SGTC_799|Compound:1310-0557|FD-Score:-4.67|P-value:5.89E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:-5.74|P-value:3.28E-5 ID:SGTC_936|Compound:3005-4620|FD-Score:-6.30|P-value:5.93E-6 ID:SGTC_949|Compound:1165-0505|FD-Score:4.73|P-value:5.06E-4 ID:SGTC_1062|Compound:mesoridazine|FD-Score:5.68|P-value:3.98E-5 ID:SGTC_1105|Compound:epirubicin|FD-Score:4.45|P-value:9.93E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:-6.49|P-value:3.18E-6 ID:SGTC_1255|Compound:0566-0045|FD-Score:-4.77|P-value:4.59E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:4.77|P-value:4.62E-4 ID:SGTC_1643|Compound:st009866|FD-Score:-4.68|P-value:5.66E-4 ID:SGTC_1742|Compound:st038235|FD-Score:-5.07|P-value:2.14E-4 ID:SGTC_1784|Compound:st039334|FD-Score:-4.62|P-value:6.56E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-5.06|P-value:2.17E-4 ID:SGTC_1834|Compound:st055981|FD-Score:5.01|P-value:2.52E-4 ID:SGTC_1853|Compound:st056710|FD-Score:4.76|P-value:4.63E-4 ID:SGTC_2206|Compound:7254556|FD-Score:5.92|P-value:1.91E-5 ID:SGTC_2216|Compound:7189485|FD-Score:5.38|P-value:9.22E-5 ID:SGTC_2229|Compound:7354935|FD-Score:5.36|P-value:9.69E-5 ID:SGTC_2393|Compound:9031399|FD-Score:5.66|P-value:4.18E-5 ID:SGTC_2399|Compound:5884133|FD-Score:-5.99|P-value:1.53E-5 ID:SGTC_2402|Compound:5553605|FD-Score:5.66|P-value:4.11E-5 ID:SGTC_2467|Compound:5474540|FD-Score:5.61|P-value:4.79E-5 ID:SGTC_2525|Compound:harmine|FD-Score:5.57|P-value:5.44E-5 ID:SGTC_2526|Compound:patulin|FD-Score:-5.28|P-value:1.20E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:6.33|P-value:5.40E-6 ID:SGTC_2611|Compound:pyromycin|FD-Score:4.73|P-value:5.10E-4 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:4.79|P-value:4.29E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:-4.65|P-value:6.05E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:-6.54|P-value:2.74E-6 ID:SGTC_2671|Compound:danthron|FD-Score:6.86|P-value:9.19E-7 ID:SGTC_2689|Compound:tacrolimus|FD-Score:-5.45|P-value:7.45E-5 ID:SGTC_2699|Compound:st077704|FD-Score:5.15|P-value:1.72E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:-14.10|P-value:5.59E-23 ID:SGTC_2778|Compound:tioconazole|FD-Score:-12.60|P-value:1.18E-18 ID:SGTC_2779|Compound:methotrexate|FD-Score:-6.49|P-value:3.20E-6 ID:SGTC_2854|Compound:9024559|FD-Score:-6.29|P-value:6.15E-6 ID:SGTC_2856|Compound:9022925|FD-Score:-5.15|P-value:1.71E-4 ID:SGTC_2921|Compound:7974240|FD-Score:-5.69|P-value:3.81E-5 ID:SGTC_2966|Compound:9084041|FD-Score:-5.63|P-value:4.48E-5 ID:SGTC_3124|Compound:9127567|FD-Score:5.01|P-value:2.46E-4 ID:SGTC_3187|Compound:9109394|FD-Score:-5.00|P-value:2.55E-4 ID:SGTC_3200|Compound:9114385|FD-Score:-4.96|P-value:2.83E-4 ID:SGTC_3204|Compound:9114183|FD-Score:-5.11|P-value:1.89E-4 ID:SGTC_3329|Compound:9141233|FD-Score:-5.00|P-value:2.52E-4

Top fitness defect scores for YOR224C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2671 danthron 6.86 100.0 µM 9.19E-7 DNA damage response 5 3.39E-12 0.47
2 SGTC_102 gallobenzophenone 6.82 83.6 µM 1.07E-6 redox potentiating 2 4.59E-12 2.29
3 SGTC_2544 sphondin 6.33 68.5 µM 5.40E-6 RNA pol III & RNase P/MRP 16 1.22E-10 0.16
4 SGTC_2206 7254556 5.92 147.0 µM 1.91E-5 60S ribosome export 2 1.57E-9 1.02
5 SGTC_221 trifluoperazine 5.87 10.3 µM 2.24E-5 NEO1-PIK1 16 2.16E-9 0.10
6 SGTC_1062 mesoridazine 5.68 14.2 µM 3.98E-5 2 6.91E-9 1.55
7 SGTC_2402 5553605 5.66 200.0 µM 4.11E-5 1 7.36E-9 1.18
8 SGTC_2393 9031399 5.66 185.9 µM 4.18E-5 RNA processing & uracil transport 3 7.62E-9 0.32
9 SGTC_93 0109-0045 5.64 59.2 µM 4.38E-5 DNA damage response 3 8.35E-9 0.12
10 SGTC_2467 5474540 5.61 200.0 µM 4.79E-5 1 9.99E-9 0.90
11 SGTC_2525 harmine 5.57 47.9 µM 5.44E-5 ergosterol biosynthesis 2 1.30E-8 0.31
12 SGTC_2216 7189485 5.38 200.0 µM 9.22E-5 1 3.74E-8 0.99
13 SGTC_2229 7354935 5.36 190.0 µM 9.69E-5 RSC complex & mRNA processing 14 4.13E-8 0.37
14 SGTC_648 0375-0473 5.27 33.3 µM 1.24E-4 2 6.74E-8 0.63
15 SGTC_2699 st077704 5.15 54.6 µM 1.72E-4 endomembrane recycling 3 1.31E-7 0.66
16 SGTC_3124 9127567 5.01 49.5 µM 2.46E-4 DNA damage response 1 2.67E-7 0.50
17 SGTC_1834 st055981 5.01 52.8 µM 2.52E-4 RPP1 & pyrimidine depletion 4 2.79E-7 0.14
18 SGTC_503 tyrphostin 8 4.87 294.0 µM 3.57E-4 TSC3-RPN4 6 5.63E-7 0.50
19 SGTC_91 0417-1751 4.86 58.4 µM 3.61E-4 Golgi 8 5.76E-7 0.42
20 SGTC_234 nsc-17383 4.84 10.7 µM 3.82E-4 ERG2 2 6.43E-7 0.72

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.155 1.75E-19 YDL150W RPC53 het RNA polymerase III subunit C53
0.154 2.80E-19 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.138 9.42E-16 YHL015W RPS20 het Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
0.131 2.87E-14 YOR157C PUP1 het Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
0.124 6.40E-13 YPL237W SUI3 het Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
0.124 7.75E-13 YPL142C_d YPL142C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
0.122 1.46E-12 YOR319W HSH49 het U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
0.121 2.10E-12 YML031W NDC1 het Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
0.120 3.89E-12 YBR154C RPB5 het RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
0.117 1.31E-11 YOR340C RPA43 het RNA polymerase I subunit A43
0.115 2.78E-11 YOL040C RPS15 het Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
0.114 3.72E-11 YDR225W HTA1 hom Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
0.107 5.65E-10 YDR429C TIF35 het eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
0.106 6.70E-10 YPR136C_d YPR136C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
0.105 9.56E-10 YER025W GCD11 het Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met